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The semi-operation of preservation the content of historical audio documents: The processes of identification, itemization, conservation, cataloguing, protective reformatting of audio and picture content and long-term archiving using the example of standard gramophone records
Šír, Filip ; Mejzr, Martin
The semi-operation system focuses on preservation of historical sound recordings recorded on 78rpm gramophone records. The system represents a comprehensive set of procedures and tools that correspond to the pillars of the workflow of the Sound Lab of the National Museum, ensuring the physical protection of gramophone records, the creation of descriptive metadata, the digital reformatting of their content and form, making them available through a digital library and storing digitized data for the purpose of long-term preservation. The chain of activities, methods and instruments within the semi-operation system aims to effectively and comprehensively identify, protect, digitize and make available the given content of historical audio carriers. The semi-operation system was tested as part of processing the collections of the National Museum and the Municipal Library in Prague.
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Evaluation of root system architecture and osmotic adaptation in barley
Klem, Karel ; Findurová, Hana ; Urban, Otmar ; Holub, Petr
Drought tolerance based on improving root system architecture, in particular by increasing rooting depth, increasing root density at deeper layers, or osmotic adjustment, is one of the most effective ways of adapting to drought, especially in conditions where sufficient water remains in the deeper soil layers during the dry season. Moreover, osmotic adjustment is a trait that allows plants to take up water even when water availability is limited, including the deeper soil layers. On the other hand, however, these are traits that are very difficult to evaluate, given their hidden nature or the need to laboriously excavate roots growing in the soil. This is also the reason why, despite their considerable importance, these features have long been overlooked. Breeding for these traits is done only indirectly by evaluating yield response to drought or indirect physiological or morphological parameters. \nIn this methodology, three methods for evaluating root system architecture or osmotic adjustment are presented which allow selection for specific root system architecture traits or osmotic adjustment, to be carried out on a large number of genotypes, using a relatively simple method based on the cultivation of roots on black filter paper, either hydroponically or aeroponically. This method allows a detailed analysis of root system architecture parameters that are important for drought tolerance and also a relatively easy test of osmotic adjustment by inducing osmotic stress with polyethylene glycol (PEG) solution. Both methods have been successfully validated, in the first case in a model experiment with nutrient deficiency, in which the effect on root system architecture is confounded, and in the second case in a set of barley genetic resources and varieties in which previously obtained information on drought tolerance of some genotypes was confirmed. \nAnother of the methods presented is the cultivation of roots in rhizoboxes filled with sand with a grid of spikes that hold the roots in the position in which they grew when washed. This method again allows the root system architecture to be assessed, but in this case under conditions that more closely match real soil conditions. The method was validated in three barley genotypes with contrasting root system vigor. The method used not only demonstrated the expected differences in rooting depth and root density distribution but also showed a relationship to the physiological response to reduced water availability in terms of photosynthetic CO2 assimilation rate. \nTherefore, all the methods presented here are suitable tools to help breeders in the selection of drought-tolerant barley genotypes.
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Phenotyping spring barley for drought tolerance
Klem, Karel ; Findurová, Hana ; Panzarová, K. ; Pytela, J. ; Trtílek, M. ; Holub, Petr
Drought tolerance is a complex of traits that vary depending on the type, severity, and timing of drought. Therefore, a relatively comprehensive assessment of the morphological and physiological phenotypic response to drought is necessary. This methodology focuses on the development of procedures and evaluation of the complex phenotypic response of spring barley genotypes to drought using non-invasively measured parameters based on red-green-blue (RGB) imaging, thermal infrared imaging, and chlorophyll fluorescence imaging measured within an automated phenotyping platform. The development of the methodology was based on an experiment in which the dynamic changes in the response of six barley genotypes, representing a wide range of drought tolerance (from a very sensitive to a very tolerant genotype), to continuous drying until the point of wilting and subsequent re-watering were monitored. Three critical time points during drought and re-watering were identified, key for detecting drought tolerance: i) reaching 50% of available soil water, ii) reaching wilting point, iii) full plant recovery after re-watering. However, the different imaging-based parameters monitored showed potential for assessing drought sensitivity at different time points from the onset of drying or recovery. The correlation with relative yield response increased progressively for the side projected leaf area (SPA) and reached a maximum at the point of complete recovery after re-watering. The actual quantum yield of photosystem II (ΦPSII) showed the highest correlation with relative grain yield around the wilting point. In contrast, the relative difference of leaf temperature to ambient air temperature (Tdiff) showed a high correlation with yield response to drought earlier, as early as at the point of reaching 50% of the level of soil water available to plants. The highest correlations with relative yield response were obtained for RGB colour analysis at the wilting point and after recovery, especially for the relative proportion of khaki, beige, dark green, and olive-green hues. Multiple regression with parameters providing a Pearson correlation coefficient R > 0.5 slightly improved the estimation of the relative yield response to drought, but mainly provided a significant improvement in the estimation of absolute grain yield under drought stress, with an estimation accuracy of around 90% in both cases. This methodology shows that the use of a combination of phenotyping methods in characterising different morphological and physiological traits not only allows the assessment of drought tolerance (based on relative yield response to drought), which is crucial for the selection of genetic resources for subsequent breeding, but also allows the testing of the yield performance of new genotypes under drought stress.
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The methodology for evaluation of environmental education programs
Činčera, Jan
The methodology may be used by public administration officials who evaluate both formal and informal environmental education programs for various reasons (incl. making decisions on the distribution of funds to support formal and informal environmental education programs)., The results can than be used by those who implemented the program to improve it or by its external evaluators such as the donor to asses its efficiency. The aim of the presented methodology is to define the basic terminology for evaluating environmental education programs, describe the basic stages of the evaluation cycle, characterize the basic alternative procedures for evaluating programs, and finally, to identify the basic requirements for evaluation research. Development of the methodology is based on the assumption that the evaluation research in the medium term will become an integral part of environmental education programs. To achieve this objective requires that officials are familiar with the theme and gain a basic understanding of the opportunities and needs of the assessment procedures.
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Methods for the detection of Paenibacillus larvae and Melissococcus plutonius using real-time PCR
Hrabák, Jaroslav ; Vlková, Kateřina ; Erban, Tomáš
The methodology is intended for diagnostic laboratories focused on bee infections. Detection of the causative agents of American foulbrood (Paenibacillus larvae) and European foulbrood (Melissococcus plutonius) is essential to prevent the spread of those highly contagious infections of a honey bee (Apis mellifera) diseases. In case of the suspicion, an examination of bee colonies in protective zones of the disease is indicated. The second possibility of use is the confirmation of the infection based on the detected clinical symptoms in the bee colony. The main target user in the Czech Republic are laboratories investigating animal diseases for the State Veterinary Administration (SVS) (accredited laboratories) in case of suspected disease and investigations in protective zones. Other target users are laboratories investigating bee colonies for reasons of preventive surveillance based on the request of beekeepers. The PCR method enables fast and reliable detection of P. larvae and M. plutonius from different samples taken from the bee colony. Compared to classic microbiological techniques, it allows to provide a result within a few hours of receiving the sample in the laboratory. In this way, it is possible to ensure, above all during the period of intense flight activity of bees, early intervention in bee colonies suspected of the occurrence of the mentioned diseases and to prevent the spread of the disease to nearby habitats of bee colonies.
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Development of a procedure for the analysis of the T-cell response against SARS-CoV-2 virus
Šmahel, Michal ; Pavliš, Oto ; Beran, Ondřej ; Bohoněk, Miloš ; Tachezy, Ruth ; Holub, Michal ; Poláková, Ingrid ; Saláková, Martina ; Schreiberová, Lucie
In 2020, the SARS-CoV-2 coronavirus caused a pandemic of covid-19 disease. To detect the cellular response induced by infection with this virus, a highly sensitive ELISPOT assay has been developed that detects specific interferon gamma-producing T lymphocytes after stimulation with synthetic peptides derived from viral antigens. This assay allows the detection of memory T cells directed against the viral spike (S) protein used for vaccination as well as against other viral proteins. This method can be applied to the analysis of the immune response in individuals after covid-19 and SARS-CoV-2 vaccination and may thus be used to determine the immune preparedness of emergency workers.
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Methodology for increasing the capacity of local actors for sustainable development of the region
Dlouhá, Jana ; Pospíšilová, Marie ; Petiška, Eduard ; Dlouhý, Jiří ; Macháčková Henderson, Laura
An analysis of the needs of rural and small communities in terms of sustainability shows that the preconditions for initiating change are, among others, in the social sphere: these include social capital (trust, developed relationships between actors, joint activities) and education appropriate to the conditions of the region (shaping local identity). The Methodology proposes how relationships and cooperation in a given place can be shaped and subsequently used to improve the quality of life in all its dimensions - social, environmental and economic. It shows practices for shaping local culture and lifestyle, presents the experience of the Movement for Change, recapitulates the principles of social entrepreneurship, and refers to existing forms of cooperation in community projects. It shows that opportunities for education, especially based on locally embedded learning, are lacking so that a shared understanding of sustainability principles in the regions to ensure a general awareness of local UR resources and opportunities is missing.
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Methodology of development of case studies for Higher Education and as Open Educational Resources (OER)
Dlouhá, Jana ; Dlouhý, Jiří ; Kapitulčinová, Dana
The methodology of development of regional case studies of Sustainable Development for Higher Education is aimed at the promotion of the use of practical cases in teaching; it should lead to the development of research tools relevant to local environmental issues and issues of sustainable development of the region, and thus emphasizes the importance of the regional context and specifics. The publication of the cases in the form of open educational resources (OER) enables access to these materials for regionally oriented education at earlier stages of the education system, and can be used for the presentation of regional specifics, and may also serve to promote dialogue concerning the development of the territory between the different social actors. The methodology outlines the procedure for creating educational case studies and shows how they are used in university teaching. Because the methodology is based on qualitative research methods working with direct experience, discussion also addresses the ways and means of creating a knowledge base as an open educational resources (OER), to facilitate the accumulation of this experience. The methodology takes into account the requirement for continuity of learning at different educational levels and gives a brief overview of similar approaches applied at lower levels of the education system; it also shows how the results can be applied in practice. The methods of case study development and use are based on the authors' own teaching experience, and on its theoretical reflection. The methodology clearly summarizes the assumptions for teaching sustainable development issues from a regional perspective; it offers the possibility of linking university teaching and research with practice, which should lead to an increased competence in addressing local issues of sustainable development in cooperation with universities.
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Methodology of text creation in open an internet space for higher education through e-learning
Dlouhá, Jana ; Braniš, Martin ; Dlouhý, Jiří ; Jančaříková, Kateřina ; Jančařík, Antonín
The methodology is intended to provide a framework for the use of open web space as a tool for teaching students at the university level, in e-learning or blended learning courses, and in various disciplines with an active and problem-oriented learning component which is desirable from the perspective of education for sustainable development. The virtual space (Wiki), the so-called "social web", provides an interactive environment and supports social skills such as cooperation, self-expression, and communication. This particular methodology is based on the concept of "academic literacy" where students develop their skills along with the writing process. MediaWiki software was used to stimulate meta-cognitive skills, the organization of the writing process, and critical thinking (with regard to resources); a model for an academic environment was presented there. Instructions available in the wiki support pre-writing, a resource search, the writing of a literature review & annotation, posing a research question, developing of text, and peer review. The method proved to be useful in practice; it provided evidence of student work and the results are available for future generations of students.
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