|
|
Zastoupení mastných kyselin ve vybraných cereálních potravinách
Kučerová, Kateřina
The diploma thesis consists of theoretical and practical part. In the theoretical part it deals with lipids and their role in human nutrition. I also characterized fatty acids and evaluated their health effects. Subsequently, I studied the theoretical basis of chromatographic methods focusing on gas chromatography, its instrumentation and evaluation. In the practical part I analysed the selected assortment of müsli bars by several producers. I focused on the quality of some müsli bars available on the Czech market, focusing mainly on fat content and fatty acid composition. As far as preparation of samples are concerned, it was necessary to lyophilize them first and then Soxhlet extraction followed. The actual determination of fatty acids was carried out using the method of gas chromatography and the results were statistically evaluated.
|
|
|
Preparation and evaluation of lipid based nanoparticles as drug carriers
Kučerová, Kateřina ; Holas, Ondřej (advisor) ; Vraníková, Barbora (referee)
Charles University in Prague, Faculty of Pharmacy in Hradci Králové Department of: Pharmaceutical Technology Supervisor: PharmDr. Ondřej Holas, Ph.D. Consultant: Mgr. Jana Kubačková Student: Kateřina Kučerová Title of thesis: Preparation and evaluation of lipid based nanoparticles as drug carriers Solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC) are promising drug delivery systems. Their capability to encapsulate both hydrophilic and lipophilic molecules, biocompatibility and biodegradability of lipids make them a suitable alternative for well-established drug carries. The aim of this thesis was to determine suitable ratios of composition of nanoparticles with acceptable properties (especially reduced size and polydispersity, high zeta potential absolute values), to investigate status and thermodynamic behaviour of the nanoparticles and lipids used and to examine drug encapsulation efficiency. Nanoprecipitation method was used to prepare nanoparticles from stearic acid as a solid lipid and in the case of NLC preparation isopropyl myristate as a liquid lipid was used. Kolliphor® P 188 as a surfactant and Span® 20 as a co-surfactant were the best choice to meet intended characteristics. It was shown that usually lower the concentration of surfactant and co-surfactant was the...
|
|
| |
|
|
Chemická konzervace potravin a její vliv na lidský organismus
Kučerová, Kateřina
This thesis deals with the problems of chemical preservation, while the first section provides an overview of all conservation methods. The second section summarizes the findings of the general conditions of use and approval of preservatives. Below are the reports permitted or prohibited chemicals that are added to foods during their production. Reports include the characteristics, uses and effects of these substances on the human body. At the same time focus on Czech and European legislation, which gives the values of maximum allowable amount that may be added to food.
|
|
|
A child in an armed conflict in the light of Art. 38 of the Convention on the Rights of the Child
Kučerová, Kateřina ; Balaš, Vladimír (advisor) ; Ondřej, Jan (referee)
A Child in an Armed Conflict in the Light of Article 38 of the Convention on the Rights of the Child The thesis aims to transparently elaborate the topic of protection of children in armed conflicts pursuant to the Article 38 of the Convention on the Rights of the Child as well as identification of the main problems associated with the topic and efforts to suggest their solutions. The thesis also focuses on monitoring and enforcement mechanism of individual international instruments. The body of the thesis is divided into four parts, each of several chapters. The first part of the thesis deals with the development of protection of children in armed conflicts until the adoption of the Convention on the Rights of the Child, and relevant international law documents on the protection of children in armed conflicts. The first part also includes a chapter with the identification of the main problems associated with the protection of children. Second part of the thesis concentrates on the analysis of various instruments of international law regarding the protection of children, that is, of course, the Convention on the Rights of the Child and its Optional Protocols, and following documents regarding protection of children based on this Convention, i.e. The Convention of the International Labour Organization...
|
|
|
Development of HPLC method for determination of vancomycine in clinical research
Kučerová, Kateřina ; Kujovská Krčmová, Lenka (advisor) ; Matysová, Ludmila (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Kateřina Kučerová Supervisor: RNDr. Lenka Kujovská Krčmová, Ph.D. Title of diploma thesis: Development of HPLC method for determination of vancomycine in clinical research The aim of this diploma thesis was to develop optimal conditions for the chromatographic determination of vancomycin for clinical routine practice. The chromatographic separation was carried out by KinetexTM C18 column, 2.6 μm particle size, 50 × 4.6 mm (Phenomenex, USA) in combination with potassium phosphate buffer (pH 4.5) and acetonitrile (90 : 10, v/v) as the mobile phase. As internal standard was chosen cefuroxime. For analytes determination UV detection at 220 nm wavelength was applied. After optimization of separation conditions, the method was applied to biological material. Samples were easily modified using the protein precipitation. The method was partially validated. Keywords: vancomycin, HPLC-UV, plasma samples
|
|
|
Social Rehabilitation as a Tool to Increase the Independence of Persons with Visual Disabilities
KUČEROVÁ, Kateřina
This thesis looks at social rehabilitation courses designed for visually impaired people. Through social rehabilitation, people learn to do various activities which help them achieve independence, self-sufficiency and independence. The study aims to map out the experience of those who have completed a social rehabilitation course and whether it has helped them achieve a more independent life. It will also ascertain the reasons which led them to undertake the course and compare the opinions of clients with the opinions of those who run the courses. To begin with, the theoretical section of the study will describe visual perception, visual impairments and their sensory compensation. It further defines social rehabilitation and describes specific social rehabilitation courses. Trained instructors lead these courses and are in intensive contact with their clients. As such, a related subchapter describes the relations between instructors and clients. Also looked at is communication with people with visual impairments, which is highly specific in many cases. The practical section utilises a qualitative survey based on semi-structured interviews. The research survey comprises two parts, and I focus on the opinions of those who have completed the course in the first part, having spoken to 6 such people, while the second part of the investigation was undertaken amongst the course instructors (teachers). The interviews were undertaken at two organisations providing services to visually impaired people. The research results suggest that completing social rehabilitation courses has helped people increase their independence and thus improve their quality of life. The courses met the expectations of clients. The opinions of clients and instructors concurred to a large extent, with everyone seeing the courses as of great benefit. The deciding factors for beginning the courses were two fundamental needs the need for independent movement and the need to acquire new information, thus to learn something new. The research results can serve anyone who wishes to find out more about the life of people with visual impairments. They will also find out about special courses with which people with visual impairments can look after themselves and their family relatively independently.
|
|
|
History, development and present breeder trends in aquaristics
Kučerová, Kateřina ; Hanel, Lubomír (advisor) ; Andreska, Jan (referee)
History, development, and present breeding trends in aquaristics The presented diploma thesis is based on empirical research, and deals with the history, development, and current trends in aquaristics. The theoretical part is focused on qualitative content analysis of relevant specialized literature. This thesis defines history and development of aquaristics in the world, as well as in our country, and compares current and past aquarium equipment and methods. In this work are presented and described current trends in modern aquaristics. The text is complemented by contemporary drawings and layouts of historical and recent equipment and technology. The research part is focused on data obtained from quantitative content analysis of aquarium publications, where is observed frequency of mentions in the text of particular species of aquarium fish. At the same time the results are compared with the content analysis of statistical data of aquaristics portals aimed at the real preferences of breeders. From the conclusion results that in their articles the authors preferred some of the selected families in each of the examined periods. Most preferred family was Cichlidae, Characidae and Cyprinidae. However, often presented species of aquarium fish do not correlate with the real preferences of modern...
|
|
|
A Comparative Analysis of Foreign News in the Main News Sessions of TV Stations ČT 1, Nova and Prima in 2010
Kučerová, Kateřina ; Trampota, Tomáš (advisor) ; Nečas, Vlastimil (referee)
Diploma thesis "A Comparative Analysis of Foreign News in the Main News Sessions of TV Stations ČT1, TV Nova and Prima TV in 2010" studies the way of producing foreign news by Czech TV stations in the year 2010. Also, it focuses on the question which countries were mentioned. The introductory part discusses the Czech and foreign literature and offers the overview of TV news production, international flow of communication and factors which influences the production of foreign news. It also introduces the main news sessions Události, Televizní noviny and Zprávy TV Prima. The analysis of foreign news service is divided into two parts, first inquires into differences in used types of reports, their representation in the main news sessions, the topical structure and other characteristics connected with the production of news. Then, the second part concentrates on the states which were mentioned in the foreign news and focuses on the impacts influencing the production of foreign news by the Czech TV stations. For both parts of the analysis methods of quantitative research were used. The thesis also studies the differences between the main news sessions of private TV channels TV Nova and Prima TV and ČT1 which offer public service broadcasting. The final part summarizes the results of the quantitative...
|
|
| |
guest ::
RSS feed.