National Repository of Grey Literature 105 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
Treatment of biological samples before HPLC analysis - determination of alfa-tocoferol in erythrocyte membrane
Pospíchalová, Naďa ; Solich, Petr (advisor) ; Matysová, Ludmila (referee)
α-Tocopherol is the principal membrane antioxidant in mammalian cells, although antioxidant is not the only mechanism of this vitamin. Many studies consider erytrocyte α-tocopherol levels to be more suitable to assess the tocopherol status of organism than its plasma levels. This thesis presents erytrocyte membranes α-tocopherol HPLC-UV analysis with diferential ultracentrigation and solid phase extraction sample pretreatment. Red cell samples were ultracentrifuged (288 000 × g, 3 minutes, 4 řC) in the presence of butylated hydroxytoluene (BHT), D-mannitol, HEPES and CaCl2. Then α-tocopherol was extracted from erytrocyte membranes by solid phase extraction with n-hexane in the presence of ascorbic acid and tocopherol acetate internal standard. The extract was dissolved in methanol and separated on the monolithic column Chromolith Performance RP-18e (100  4.6 mm) using a 100% methanol as the mobile phase. The absorbance of α-tocopherol was measured at 295 nm wavelength.
HPLC method development of active substances in nutraceuticals
Jägerová, Kateřina ; Šatínský, Dalibor (advisor) ; Matysová, Ludmila (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Bc. Kateřina Jägerová Supervisor: Doc. RNDr. Dalibor Šatínský, Ph.D. Title of Diploma Thesis: HPLC method development of active substances determination in nutraceuticals A high performance liquid chromatography (HPLC) method with diode array detection (DAD) was used and validated for the simultaneous determination rutin, troxerutin, diosmin and hesperidin. The method was used for direct determination of this flavonoids in pharmaceutical preparations and nutraceuticals, Cilkanol (Zentiva), Detralex (Les Laboratoires), Hemodin Prebio Forte (Valosun a. s.), MobiVen Micro (Vulm cz a.s.). Using Ascentis Express RP - Amide (10 cm x 3.0 mm, 2.7 μm) Supelco Analytical column, the following conditions were chosen as optimal: mobile phase acetonitril/water 30/70 containing acetic acid for pH adjustment to value pH 3. Method development was performed within a temperature of 50řC and pressure of 18.6 MPa. The flavonoids were detected by diode array detector (DAD) at the wavelength of 283 nm and 255 nm. Keywords: high performance liquid chromatography (HPLC), flavonoids, rutin, troxerutin, diosmin, hesperidin, nutraceuticals
Difficulty of urinary neopterin and creatinine determination using high-performance liquid chromatography with focus on clinical practice
Kepka, Zdeněk ; Kujovská Krčmová, Lenka (advisor) ; Matysová, Ludmila (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Bc. Zdeněk Kepka Supervisor: Assoc. Prof. RNDr. Lenka Kujovská Krčmová, Ph.D. Title of the diploma thesis: Difficulty of urinary neopterin and creatinine determination using high-performance liquid chromatography with focus on clinical practice This diploma thesis deals with the determination of neopterin and creatinine in urine samples by high performance liquid chromatography. The theoretical part summarizes information about the substances and their analysis. It also includes a review of methods published in the literature. The method of high-performance liquid chromatography including types of stationary phases and chromatographic principles used in the experimental part of this work is briefly introduced. The separation properties of six chromatographic columns for the determination of neopterin and creatinine in urine were verified in the experimental part of the thesis. Columns were compared by the following parameters: repeatability, resolution, peak symmetry, and column efficiency. The composition of the mobile phase was optimized for selected columns. The mobile phase was a phosphate buffer with a concentration of 15 mmol/l and pH 6.5 in most cases. Creatinine detection was performed by...
Usage of HPLC and UHPLC-MS/MS techniques for clinical research
Matoušová, Kateřina ; Matysová, Ludmila (advisor) ; Doležal, Rafael (referee) ; Kučera, Radim (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Mgr. Kateřina Kučerová Supervisor: assoc. prof. PharmDr. Ludmila Matysová, Ph.D. Consultant: assoc. prof. RNDr. Lenka Kujovská Krčmová, Ph.D. Title of Dissertation Thesis: Usage of HPLC and UHPLC - MS/MS techniques for clinical research The dissertation thesis is dealing with the extraction techniques and with development, optimization and validation of chromatographic methods for the determination of omeprazol which is drug from the group of proton pump inhibitors, and retinol as a new potential biomarker of kidney damage. The theoretical part of the thesis is focused on detailed description of the individual analytes, their clinical significance, options of determination and the matrix in which the target analytes were determined. Furthermore, the preparation of the sample before the analysis is discussed and validation recommendations related to chromatographic methods used in both pharmaceutical analysis and bioanalysis are also mentioned. In the experimental part the development, optimization and validation of UHPLC methods and their subsequent use are described in detail. The pharmaceutical-analytical part of the work describes the determination of omeprazole in new suspension preparations,...
Usage of HPLC and UHPLC-MS/MS techniques for clinical research
Kučerová, Kateřina ; Matysová, Ludmila (advisor) ; Doležal, Rafael (referee) ; Kučera, Radim (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Mgr. Kateřina Kučerová Supervisor: assoc. prof. PharmDr. Ludmila Matysová, Ph.D. Consultant: assoc. prof. RNDr. Lenka Kujovská Krčmová, Ph.D. Title of Dissertation Thesis: Usage of HPLC and UHPLC - MS/MS techniques for clinical research The dissertation thesis is dealing with the extraction techniques and with development, optimization and validation of chromatographic methods for the determination of omeprazol which is drug from the group of proton pump inhibitors, and retinol as a new potential biomarker of kidney damage. The theoretical part of the thesis is focused on detailed description of the individual analytes, their clinical significance, options of determination and the matrix in which the target analytes were determined. Furthermore, the preparation of the sample before the analysis is discussed and validation recommendations related to chromatographic methods used in both pharmaceutical analysis and bioanalysis are also mentioned. In the experimental part the development, optimization and validation of UHPLC methods and their subsequent use are described in detail. The pharmaceutical-analytical part of the work describes the determination of omeprazole in new suspension preparations,...
Comparison of methods for determination of amphetamine derivates in meconium
Vrbová, Romana ; Matysová, Ludmila (advisor) ; Šatínský, Dalibor (referee)
Department: Department of Analytical Chemistry Candidate: Romana Vrbová Supervisor: doc. PharmDr. Ludmila Matysová, Ph.D. Title of the diploma thesis: Comparison of methods for determination of amphetamine derivates in meconium The aim of the bachelor thesis was to optimize a new method for the determination of amphetamine derivatives in meconium, which will be faster and simpler than the existing routine method in the toxicological laboratory of Masaryk's Hospital in Ústí nad Labem. The theoretical part of the bachelor thesis describes the main representatives of amphetamine derivatives, their effects and metabolism. Their determination in meconium is important for the diagnosis of Neonatal abstinence syndrome, which occurs in newborns of mothers using addictive substances during pregnancy. Meconium, the first stool of a newborn, is a suitable material demonstrating exposure to drugs and medication during the second and third trimesters of pregnancy. Amphetamine derivatives were determined in meconium by gas chromatography with mass detection, the principles and instrumentalization of which are another part of the bachelor's thesis. The experimental part describes the optimization of a new, more advantageous method using pentafluorobenzoyl chloride as a derivatizing agent and its comparison with...
Stability testing of benzalconium chloride stock solution by HPLC
Křesťanová, Petra ; Matysová, Ludmila (advisor) ; Sklenářová, Hana (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Petra Křesťanová Supervisor: doc. PharmDr. Ludmila Matysová, Ph.D. Title of the diploma thesis: Monitoring the stability of benzalkonium chloride stock solution by HPLC method This diploma thesis evaluates the stability of benzalkonium chloride in aqueous stock solution. The purpose of this work was to choose the most suitable conditions for storing and determine the shelf-life. This stability study was conducted by validated High Performance Liquid Chromatography method. Tested samples were prepared with pH 6.8 and 5 and stored in glass and plastic containers at room and lowered temperature in the absence of light. The stability was analysed in intervals of 0, 7, 14, 28, 60 and 90 days. All samples showed a good stability during the whole time of testing, without any significant differences. The recommended conditions for storing benzalkonium chloride solutions are room temperature and pH 6.8 in dark glass containers. These solutions can be used in the period of three months.
Difficulty of urinary neopterin and creatinine determination using high-performance liquid chromatography with focus on clinical practice
Kepka, Zdeněk ; Kujovská Krčmová, Lenka (advisor) ; Matysová, Ludmila (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Bc. Zdeněk Kepka Supervisor: Assoc. Prof. RNDr. Lenka Kujovská Krčmová, Ph.D. Title of the diploma thesis: Difficulty of urinary neopterin and creatinine determination using high-performance liquid chromatography with focus on clinical practice This diploma thesis deals with the determination of neopterin and creatinine in urine samples by high performance liquid chromatography. The theoretical part summarizes information about the substances and their analysis. It also includes a review of methods published in the literature. The method of high-performance liquid chromatography including types of stationary phases and chromatographic principles used in the experimental part of this work is briefly introduced. The separation properties of six chromatographic columns for the determination of neopterin and creatinine in urine were verified in the experimental part of the thesis. Columns were compared by the following parameters: repeatability, resolution, peak symmetry, and column efficiency. The composition of the mobile phase was optimized for selected columns. The mobile phase was a phosphate buffer with a concentration of 15 mmol/l and pH 6.5 in most cases. Creatinine detection was performed by...
Development and validation of method for determination of of Cyclosporine in biorelevant mediums
Tichoň, Matej ; Matysová, Ludmila (advisor) ; Kujovská Krčmová, Lenka (referee)
Charles university Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Matej Tichoň Supervisor: assoc. prof. PharmDr. Ludmila Matysová, Ph.D. Title of diploma thesis: Development and validation of method for determination of cyclosporine A in biorelevant mediums For the determination of cyclosporine A in biorelevant mediums, a new analytical method was developed by using of ultra-high performance liquid chromatography. The analysis was performed on a Kinetex™ 1.7 µm, C18 column, 50 x 2.1 mm (Phenomenex, USA). The mobile phase consisted of ultrapure water acidified with phosphoric acid to pH = 2.40 and mixture of methanol : acetonitrile (90 : 10) in a total ratio of 30 : 70 aqueous to organic phases. The analysis was carried out at 60 řC for 5 minutes with a constant flow rate of 0.5 ml/min. Gradient elution was used in the analysis, starting at 70 % organic phase at 0 minutes and increasing to 100 % at 5 minutes. Cyclosporine A was detected at 210 nm. The retention time of the substance was 3.08 min. Having the optimized conditions in hands, the method was fully validated. Keywords: cyclosporine A, UHPLC, biorelevant mediums

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