National Repository of Grey Literature 32 records found  1 - 10nextend  jump to record: Search took 0.01 seconds. 
Difficulty of urinary neopterin and creatinine determination using high-performance liquid chromatography with focus on clinical practice
Kepka, Zdeněk ; Kujovská Krčmová, Lenka (advisor) ; Matysová, Ludmila (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Bc. Zdeněk Kepka Supervisor: Assoc. Prof. RNDr. Lenka Kujovská Krčmová, Ph.D. Title of the diploma thesis: Difficulty of urinary neopterin and creatinine determination using high-performance liquid chromatography with focus on clinical practice This diploma thesis deals with the determination of neopterin and creatinine in urine samples by high performance liquid chromatography. The theoretical part summarizes information about the substances and their analysis. It also includes a review of methods published in the literature. The method of high-performance liquid chromatography including types of stationary phases and chromatographic principles used in the experimental part of this work is briefly introduced. The separation properties of six chromatographic columns for the determination of neopterin and creatinine in urine were verified in the experimental part of the thesis. Columns were compared by the following parameters: repeatability, resolution, peak symmetry, and column efficiency. The composition of the mobile phase was optimized for selected columns. The mobile phase was a phosphate buffer with a concentration of 15 mmol/l and pH 6.5 in most cases. Creatinine detection was performed by...
Development of new types of biocompatible hemodialysis membranes for separation of biomolecules
Kohlová, Michaela ; Solich, Petr (advisor) ; Kujovská Krčmová, Lenka (referee) ; Pena, Angelina (referee)
Charles University, Faculty of Pharmacy in Hradec Králové / University of Porto, Faculty of Pharmacy Department of Analytical Chemistry / Department of Chemical Sciences Candidate: Mgr. Michaela Kohlová Supervisor: prof. RNDr. Petr Solich, CSc. Consultants: prof. Maria de Conceição Branco Montenegro assoc. prof. Alice Santos-Silva Title of dissertation thesis: Development of new types of biocompatible haemodialysis membranes for separation of biomolecules This dissertation thesis was focused on the development, preparation, and optimization of flat sheet polysulfone membranes, which would meet the characteristics required for membranes used for haemodialysis treatment. The thin porous membranes were prepared by spin coating method, followed by phase inversion via immersion precipitation. The composition, as well as the preparation process of the membranes, were optimized, to obtain a membrane with good mechanical performance in flow conditions, adequate selective separation ability, and biocompatibility. The prepared membranes with the appropriate characteristics were modified with bioactive compounds to minimize oxidative stress and/or inflammation, which are common complications in haemodialysis treated patients. Three different modification approaches were used to prepare the bioactive...
Analysis of drugs and potential drugs in biological material using liquid chromatography
Reimerová, Petra ; Štěrbová, Petra (advisor) ; Kujovská Krčmová, Lenka (referee) ; Žďárová Karasová, Jana (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Candidate: Mgr. Petra Reimerová Supervisor: doc. PharmDr. Petra Štěrbová, Ph.D. Title: Analysis of drugs and potential drugs in biological material using liquid chromatography Bioanalytical methods play important role during development of new drugs. This is because determining the concentration of the drug of interest in biological matrices can help to reveal its mechanism of action, determine the values of basic pharmacokinetic parameters or study the fate of a potential drug during in vitro experiments. Considering the complexity of biological samples and low concentrations of studied compounds, the sample preparation prior to analysis is really important. Miniaturized techniques derived from standard extraction methods have significant presence in modern bioanalysis, mainly because they decrease both required sample volume and consumption of laboratory material to a minimum, and they enable automatization. One of the main areas of drug discovery is currently the treatment of cancer. Unfortunately, development of highly efficacious drugs and prolongation of patient survival have resulted in the risk of developing late complications associated with anticancer treatment...
Analysis of drugs and potential drugs in biological material using liquid chromatography
Reimerová, Petra ; Štěrbová, Petra (advisor) ; Kujovská Krčmová, Lenka (referee) ; Žďárová Karasová, Jana (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Candidate: Mgr. Petra Reimerová Supervisor: doc. PharmDr. Petra Štěrbová, Ph.D. Title: Analysis of drugs and potential drugs in biological material using liquid chromatography Bioanalytical methods play important role during development of new drugs. This is because determining the concentration of the drug of interest in biological matrices can help to reveal its mechanism of action, determine the values of basic pharmacokinetic parameters or study the fate of a potential drug during in vitro experiments. Considering the complexity of biological samples and low concentrations of studied compounds, the sample preparation prior to analysis is really important. Miniaturized techniques derived from standard extraction methods have significant presence in modern bioanalysis, mainly because they decrease both required sample volume and consumption of laboratory material to a minimum, and they enable automatization. One of the main areas of drug discovery is currently the treatment of cancer. Unfortunately, development of highly efficacious drugs and prolongation of patient survival have resulted in the risk of developing late complications associated with anticancer treatment...
Development of new types of biocompatible hemodialysis membranes for separation of biomolecules
Kohlová, Michaela ; Solich, Petr (advisor) ; Kujovská Krčmová, Lenka (referee) ; Pena, Angelina (referee)
Charles University, Faculty of Pharmacy in Hradec Králové / University of Porto, Faculty of Pharmacy Department of Analytical Chemistry / Department of Chemical Sciences Candidate: Mgr. Michaela Kohlová Supervisor: prof. RNDr. Petr Solich, CSc. Consultants: prof. Maria de Conceição Branco Montenegro assoc. prof. Alice Santos-Silva Title of dissertation thesis: Development of new types of biocompatible haemodialysis membranes for separation of biomolecules This dissertation thesis was focused on the development, preparation, and optimization of flat sheet polysulfone membranes, which would meet the characteristics required for membranes used for haemodialysis treatment. The thin porous membranes were prepared by spin coating method, followed by phase inversion via immersion precipitation. The composition, as well as the preparation process of the membranes, were optimized, to obtain a membrane with good mechanical performance in flow conditions, adequate selective separation ability, and biocompatibility. The prepared membranes with the appropriate characteristics were modified with bioactive compounds to minimize oxidative stress and/or inflammation, which are common complications in haemodialysis treated patients. Three different modification approaches were used to prepare the bioactive...
Difficulty of urinary neopterin and creatinine determination using high-performance liquid chromatography with focus on clinical practice
Kepka, Zdeněk ; Kujovská Krčmová, Lenka (advisor) ; Matysová, Ludmila (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Bc. Zdeněk Kepka Supervisor: Assoc. Prof. RNDr. Lenka Kujovská Krčmová, Ph.D. Title of the diploma thesis: Difficulty of urinary neopterin and creatinine determination using high-performance liquid chromatography with focus on clinical practice This diploma thesis deals with the determination of neopterin and creatinine in urine samples by high performance liquid chromatography. The theoretical part summarizes information about the substances and their analysis. It also includes a review of methods published in the literature. The method of high-performance liquid chromatography including types of stationary phases and chromatographic principles used in the experimental part of this work is briefly introduced. The separation properties of six chromatographic columns for the determination of neopterin and creatinine in urine were verified in the experimental part of the thesis. Columns were compared by the following parameters: repeatability, resolution, peak symmetry, and column efficiency. The composition of the mobile phase was optimized for selected columns. The mobile phase was a phosphate buffer with a concentration of 15 mmol/l and pH 6.5 in most cases. Creatinine detection was performed by...
Development and validation of method for determination of of Cyclosporine in biorelevant mediums
Tichoň, Matej ; Matysová, Ludmila (advisor) ; Kujovská Krčmová, Lenka (referee)
Charles university Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Matej Tichoň Supervisor: assoc. prof. PharmDr. Ludmila Matysová, Ph.D. Title of diploma thesis: Development and validation of method for determination of cyclosporine A in biorelevant mediums For the determination of cyclosporine A in biorelevant mediums, a new analytical method was developed by using of ultra-high performance liquid chromatography. The analysis was performed on a Kinetex™ 1.7 µm, C18 column, 50 x 2.1 mm (Phenomenex, USA). The mobile phase consisted of ultrapure water acidified with phosphoric acid to pH = 2.40 and mixture of methanol : acetonitrile (90 : 10) in a total ratio of 30 : 70 aqueous to organic phases. The analysis was carried out at 60 řC for 5 minutes with a constant flow rate of 0.5 ml/min. Gradient elution was used in the analysis, starting at 70 % organic phase at 0 minutes and increasing to 100 % at 5 minutes. Cyclosporine A was detected at 210 nm. The retention time of the substance was 3.08 min. Having the optimized conditions in hands, the method was fully validated. Keywords: cyclosporine A, UHPLC, biorelevant mediums
Development and validation of UHPLC method for determination of miconazole, econazole and its impurities in solid dispersions
Arnoltová, Kristýna ; Matysová, Ludmila (advisor) ; Kujovská Krčmová, Lenka (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Kristýna Arnoltová Supervisor: doc. PharmDr. Ludmila Matysová, Ph.D. Title of the diploma thesis: Development and validation of UHPLC method for determination of miconazole, econazole and its impurities in solid dispersions The aim of the submitted thesis was to create a new analytical method for the determination of miconazole, econazole and its three impurities. Ultra-high performance liquid chromatography (UHPLC) was used. A KinetexTM XB C18 column, 1.7 μm particle size, 50 x 2.1 mm was used for the separation. The mobile phase was composed of the acetate buffer pH 7.8 in a mixture with methanol (80:20) and of acetonitrile with methanol (60:40), in a total ratio of 60:40. A gradient elution was used. Flow rate was set to 0.6 ml / min. Butylparaben was chosen as the internal standard. All substances were detected at a wavelength of 225nm. Total analysis time was 9 minutes. After optimization of the separation conditions, the method was partially validated and used for practical measurements to determine the content of miconazole in solid dispersions.
Separation of tocopherols using HPLC technique
Hutníková, Michaela ; Kujovská Krčmová, Lenka (advisor) ; Matysová, Ludmila (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Michaela Hutníková Supervisor: assoc. prof. RNDr. Lenka Kujovská Krčmová Ph.D. Title of diploma thesis: Separation of tocopherols using HPLC technique Vitamin E represents eight related compounds α, β, γ, δ-tocopherols (saturated phytyl side chain) and α, β, γ, δ-tocotrienols (unsaturated phytyl side chain). α-tocopherol was the most studied isomer in the past and its anti-inflammation and proliferative effect was described. Therefore most of cancer prevention trials have been focused on α-tocopherol. Present research studies have described the important role of γ and δ-tocopherols in cell proliferation, anti-inflammation and tumor burden. Also it has been demonstrated that γ-tocopherol inhibits colon, prostate, mammary and lung tumor genesis in animal models, suggesting to have a high potential in the prevention of human cancer. In this study the novel sensitive method for analysis of α, β, γ, δ-tocopherols was developed using the High Performance Liquid Chromatography (HPLC) technique. All the measurements were carried out on the chromatographic system HPLC Prominence LC 20, Shimadzu (Kyoto, Japan). During the method development core-shell columns-Kinetex (Torrance, USA) with different...
Analytical and bioanalytical evaluation of novel potential drugs from the group of iron chelators
Bureš, Jan ; Štěrbová, Petra (advisor) ; Kujovská Krčmová, Lenka (referee) ; Hroch, Miloš (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Candidate: Mgr. Jan Bureš Supervisor: Doc. PharmDr. Petra Štěrbová, Ph.D. Title of Thesis: Analytical and bioanalytical evaluation of novel potential drugs from the group of iron chelators High performance liquid chromatography (HPLC) gained its unprecedented position among bioanalytical techniques due to its effectivity and versatility. If tuned properly, it can separate complex mixtures and can be used for qualitative and quantitative analysis simultaneously, usually within a short time. Reliability of the HPLC methods is proven by their validation performed according to accepted guidelines. Iron chelation is considered to be an effective concept of treatment of various pathologies - from iron overload disease to cancer or neurodegenerative disorders. To develop new chelators for these indications it is important to investigate their structure- activity relationship, the fate of the drug in organism and its relationship to biological effects and toxicity etc. Modern analytical methods are essential tools for these studies. The first part of this work presents validated LC-UV and LC-MS methods for assay of aroylhydrazone iron chelators and related pro-drugs in biological...

National Repository of Grey Literature : 32 records found   1 - 10nextend  jump to record:
Interested in being notified about new results for this query?
Subscribe to the RSS feed.