National Repository of Grey Literature 12 records found  1 - 10next  jump to record: Search took 0.01 seconds. 
Automated object tracking using robotic manipulator
Zelený, Miroslav ; Ligocki, Adam (referee) ; Chromý, Adam (advisor)
This diploma thesis deals with the tracking of objects using a robotic manipulator Epson C3 and a color camera. The work describes the basic qualities of the device to be used. The OpenCV library and its wrapper EmguCV are used as software tools for computer vision. It discusses the basic issues and principles of tracking objects in the image and introduces some methods of tracking. These methods have been tested and therefore their strengths and weaknesses, which appeared during testing, are listed here. Furthermore, there is a procedure for calculating the new coordinates of the camera and the manipulator effector using homogeneous transformations. The work contains the results of testing the algorithms and their evaluation. The output of the work is a test application for the Epson C3 robot.
Pathophysiology of colorectal cancer. Colorectal cancer screening effect and the role of microRNA in pathophysiology of colorectal cancer.
Král, Jan ; Špičák, Julius (advisor) ; Keil, Radan (referee) ; Mohelníková Duchoňová, Beatrice (referee)
Colorectal cancer is a serious malignant disease with an incidence of over 1.8 million new cases per year worldwide. There are about 8 000 patients diagnosed with CRC in the Czech Republic each year, and about half of them present with an advanced disease. Screening program identifies patients in the early stages of CRC resulting in overall better prognosis and survival. There is also a lack of biomarkers of early CRC detection and of response to treatment. The first aim of our project was to conduct a national multicentre prospective observational study to evaluate the impact of CRC screening within the framework of a Czech population screening programme. Between March 2013 and September 2015, a total of 265 patients were enrolled in 12 centres across the Czech Republic. Patients were divided into screening and control groups and compared for pathology status and clinical characteristics. Screening was defined as a primary screening colonoscopy or a colonoscopy after a positive FOBT in an average-risk population. The distribution of CRC stages was significantly favourable in the screening group compared with the control group (stages 0, I and II, 63% versus 43.3%; p <0.001). The presence of distant (M1) and local metastases (N1 and N2) was significantly less prevalent in the screening group (0%,...
Identifikace pšenice seté a špaldy DNA markery
Smetana, Daniel
Spelt has recently become more popular and desirable due to the better nutritional composition of foods containing spelt, compared to traditional foods from common wheat. Both of these plants are some of the basic cereals, they are grown all over the world and are mainly used in the food and feed industries. Due to the frequent adulteration of spelt products through the use of common wheat admixtures, there is a growing interest in their differentiation. Molecular biology methods are one of the possible tools for identifying them. This work entitled “Identification of common wheat and spelt by DNA markers” was focused in this direction as well. DNA markers associated with variability in the γ-gliadin gene through PCR reaction and restriction cleavage (RFLP method) were tested and verified. Nine genotypes of the wheat genus were tested and the results were verified on polyacrylamide electrophoresis and by means of bioinformatics tools. The result shows that the polymorphism in the γ-gliadin gene can be used as an identifier for distinguishing spelt and common wheat.
A simple method for detection of Czech bolting garlics (Allium sativum L.) using microsatellite analysis: methodology for practice
Ovesná, Jaroslava ; Mitrová, Katarína ; Kučera, Ladislav
The aim of the methodology was to verify and compile a set of microsatellite markers for the purpose of verification to be used for the undisputed identification of selected types of Czech bolting garlics. The object of submitted methodology is to simplify procedure that allow to determine Czech bolting garlic using microsatellite analysis (SSR- Single Sequence Repeats), which is applicable in testing laboratories. The described method allows to identify the variety and breeding material of bolting garlic of Czech origin using DNA markers (microsatellite alleles) that are present only for this group of varieties and breeding lines. By the amplification of the genome containing the microsatellite locus by polymerase chain reaction (PCR) using specific, fluorescently labeled primers, and subsequent analysis of the lengths of PCR products. Methodology newly brings a unique set of diagnostic SSR markers, describe the evaluation and interpretation of the results.
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Methodology for detecting the presence of cranberries and lingonberries in processed products using molecular SSR markers: methodology for practice
Kučera, Ladislav ; Ovesná, Jaroslava
The subject of the methodology is a procedure for diagnosing the presence of lingonberries (Vaccinium vitis-idaea L.) and cranberries (Vaccinum macrocarpon L.) in processed products such as tea or fruit ingredients of many food products (jams, jelly, purree, tea). The methodology exploit DNA sequences that are typical for each plant species. Thus, molecular DNA markers that characterize the plant species by SSRs (single sequence repeats) are used. The methodology describes the extraction of DNA from the assumed matrix, the use of selected SSR markers and the analytical data evaluation procedure. The method can be used in any solid equipped molecular-genetic laboratory. The method is applicable for the characterization of DNA isolated from any part of the plant, fruit, and derived food or food supplements. Method describes the procedure and necessary equipment to perform the analysis. Protocol recommends how to evaluate the results.
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Chosen problems of genetic diversity of dog
The bachelor thesis is worked out in the form of the research on the theme "Selected questions from the genetic diversity of dogs". The aim of this work was processing of the current situation of scientific knowledge in the field of genetic diversity of dogs. Firstly, the origin is described in the thesis, domestication of dogs, an organisation dealing with dogs breeding and selected breeds of dogs. From the genetic site point of view, the basic terms in genetics are described genetic markers, DNA profile of dog and the methods of evaluation of genetic diversity. In the end of my bachelor thesis I outlined possible recommendations for dog breeders.
Genetic diversity of cattle in Czech Republic
The thesis deals with genetic diversity in the Czech Republic. Firstly domestication of livestock in general and then domestication of cattle is decribed. History of cattle in the Czech Republic, the importance of cattle breeding and last but not least the condition of cattle in the Czech Republic is also introduced. The thesis is also focused on biodiversity, threats to biodiversity and its conservation and the levels of diversity are also specified. Genetic markers,microsatellites, minisatellites, SNP, polymorphic structural genes and also mutations are described. The cattle breeds farmed in the Czech Republic are specified next. The National Programme, genetic resources and genetic resources in the Czech Republic are evaluated. Finally genetic resources of " česká červinka" and "český strakatý skot" are described.
Genetic diversity of chosen horse breeds
The objective of the thesis is to clarify the findings about genetical biodiversity of horses, evaluation of the diversity and description of current state of genetic resources preservation. Firstly, the history is described, domestication of horses, breeds and biodiversity. From the genetic point of view, the thesis contains description of the genetical diversity, microsatellites, markers and mitochondria. There are described genetic sources and their preservation. Current quantities and thein progress are also mentioned.
Methodology of utilization of molecular-genetic markers of gene sequences and genetic elements in breeding and management of hop (Humulus lupulus)
Patzak, Josef ; Matoušek, Jaroslav
Tato metodika je první komplexní metodika využití molekulárně-genetických markerů pro charakterizaci genových zdrojů chmele, spolehlivou identifikaci jednotlivých genotypů chmele a systém selekce hospodářských znaků podle molekulárních markerů (MAS) ve šlechtitelském procesu chmele. Efektivní markerovací systém, kvalitativně vyšší úrovně, je založen na základě sekvencí genů a genetických elementů, získaných skríningem genomových a expresních knihoven, pro šlechtění a management chmele (Humulus lupulus).
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