National Repository of Grey Literature 1 records found  Search took 0.00 seconds. 
Identifikace pšenice seté a špaldy DNA markery
Smetana, Daniel
Spelt has recently become more popular and desirable due to the better nutritional composition of foods containing spelt, compared to traditional foods from common wheat. Both of these plants are some of the basic cereals, they are grown all over the world and are mainly used in the food and feed industries. Due to the frequent adulteration of spelt products through the use of common wheat admixtures, there is a growing interest in their differentiation. Molecular biology methods are one of the possible tools for identifying them. This work entitled “Identification of common wheat and spelt by DNA markers” was focused in this direction as well. DNA markers associated with variability in the γ-gliadin gene through PCR reaction and restriction cleavage (RFLP method) were tested and verified. Nine genotypes of the wheat genus were tested and the results were verified on polyacrylamide electrophoresis and by means of bioinformatics tools. The result shows that the polymorphism in the γ-gliadin gene can be used as an identifier for distinguishing spelt and common wheat.

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3 SMETANA, David
3 Smetana, David
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