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Testing of food authenticity using DNA analysis
Kocianová, Vanda ; Rittich, Bohuslav (referee) ; Španová, Alena (advisor)
Authenticity verification of food products is an important step before their release to the market. This testing ensures that the product contains what the label states and also confirms the medical harmlessness. One of methods possible to use for authenticity verification is polymerase chain reaction (PCR). The experimental part was focused on the DNA isolation using magnetic particles on PCR – ready quality from food product – ketchup. DNA isolated from seeds and stalk of tomato were used as a controls. Amplificalibity of DNA isolated with magnetic particles using magnetic separator and needle was compared. The method requires further optimization.
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Use of analytical methods and chemometry to verify the geographical authenticity of honey
Marková, Lucie ; Křikala, Jakub (referee) ; Diviš, Pavel (advisor)
The teoretical part of this bachelor thesis is focused on the process of producing the honey by bees, its composition and means of its adulteration or ways to detect substandard products. The experimental part describes methodes, which were used to observe the samples. Sacharides, organic acids, mineral content, the amount of dry matter, titration acidity and conductivity were meassured. Obtained data were used for differentiation the Czech and foreign honey. The result of the statistical analysis shows the calcium, copper and glucose content are suitable paramteres to differentiate the Czech honey from that externals. Theese parameters could be used to distinguish the two different geographical origin of honey. Nevertheless, the total evaluation seems to be insufficient for differentiation these two groups. The efficiency could be icreased by the including more samples for analysis, or meassuring more parameters. Finally, some of the parameters were used for quality control of honey.
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Application of analytical techniques and chemometry for authentification of wine
Pořízka, Jaromír ; Karovičová, Jolana (referee) ; Jarošová, Alžběta (referee) ; Diviš, Pavel (advisor)
This PhD. thesis is focused on application of different analytical techniques for determination of elemental composition of Moravian wine combined with analysis of selected polyphenol compounds. Two spectrometric techniques were used for elemental analysis of wine – Mass spectrometry and Optical emission spectrometry with inductively coupled plasma (ICP-MS and ICP-OES). Analysis of organic constituents of wine was evaluated by High performance liquid chromatograph with diode array detector (HPLC-DAD). Data from analysis of wine were used for construction of mathematical models for determining geographical origin of wine and for determining type of varietal wine. Data handling was performed by usage of multivariate statistical methods (cluster analysis, principal component analysis and canonical discriminant analysis). For the discrimination of wine by the geographical origin, two separate models for white and red wine were constructed. Effectiveness of discrimination of Moravian wine (with knowledge about origin) into 4 wine regions was 100 % successful for white wine and 96,43 % for red wine samples. Those models were used for cross validation test of unknown samples with result 95 % successfully classified samples of white wine and 82,15% of red wine. Besides that, mathematical model for discrimination and classification of varietal wine was made. Müller Thurgau, Riesling and Gruner Veltliner were discriminated by the usage of 8 elemental parameters. Discrimination success rate of known samples was evaluated by canonical discriminant analysis with 95,83% of correctly classified known samples and 70,83% of correctly classified unknown varietal wine (cross validation test). In addition to these part of research, impact of different grape production systems on elemental composition of wine and Vitis Vinifera was studied. The core of the study was to find the connections between the type of agriculture and chemical properties of wine and Vitis Vinifera. Two agriculture systems were assessed – Organic agriculture and Integrated pest management. . Results of this study showed that quantity and manner of pesticide application is very important factor affecting the final elemental composition of the grapevine and wine, especially the content of copper, zinc and manganese. That knowledge can be used in the future for creation of classification model of wine according to grape production system. For comparison of radical scavenging activity, electron paramagnetic resonance spectroscopy (EPR) with DPPH method was used. In the case of this study, the influence of vine growing under different farming systems on the total antioxidant activity of wine was found to be insignificant.
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Sakura, manga, kimono: Representation of Japan at Japanese themed events
Nezveda, Tomáš ; Halbich, Marek (advisor) ; Knotková - Čapková, Blanka (referee)
Sakura, manga, kimono: Representation of Japan at Japanese themed events Bc. Tomáš Nezveda Abstract This thesis studies the issue of a representation of Japan at Japanese themed events in the Czech Republic. The conclusions of this thesis are based on empirical research in three essential terrains - the Japan Information and Culture Centre of the Japanese Embassy in the Czech Republic, the Czech-Japanese Association, and the Czech anime community. Each terrain's activities are analyzed to identify images of Japan they produce for their visitors. This contextual basis is built on for a further analysis of three significant issues that arise from the research - the difference and prevalence of Japanese culture in the Czech Republic, its authenticity, and the tendency of Czech fans to idealize Japan.
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Authenticity in the perception of users of the BeReal network
Tabačíková, Kristýna ; Soukup, Martin (advisor) ; Vochocová, Lenka (referee)
The bachelor thesis "Authenticity from the Perspective of BeReal Social Media Users" examines the concept and perception of authenticity among users of the social media platform BeReal. It also focuses on the expression of identity and self-presentation within the platform's environment and investigates the motivations that drive participants in the research to use BeReal. The theoretical part of the thesis delves into the concepts of authenticity, identity, self-presentation, and sharing everyday experiences, providing the contextual framework for the study. The theoretical foundations draw from George E. Newman's categorization of authenticity, Erving Goffman's dramaturgical analysis, the exhibitionist approach to self-presentation, and social photography. The research aimed to explore how BeReal users interpret the concept of authenticity, how it manifests for them, and what it means to them. For this purpose, semi-structured in-depth interviews were conducted, and the data were subsequently analyzed using thematic analysis. The results revealed that authenticity does not hold significant importance for BeReal users, who primarily use the platform to maintain social contacts. Participants associated authenticity with aspects of ordinariness and non-aesthetics, or as a means of expressing their...
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Development of methods for determination of authenticity of cosmetic products with a plant component
Langová, Denisa ; Brázda, Václav (referee) ; Vorlová, Lenka (referee) ; Márová, Ivana (advisor)
In the final thesis, methods were developed for determining the authenticity of cosmetic products and food products with a plant component. The presence of natural ingredients in a series of model and commercial cosmetic products was verified and their authenticity was verified, too. The focus of the work was the development of a molecular-biological method for the detection of plant components (herbs, fruits, essential oils) and probiotics present in cosmetic products. Commercially available isolation kits were used for the isolation of DNA from cosmetic products and the method was optimized for the isolation of plant DNA present in cosmetic products. The presence of a plant matrix in cosmetic products was detected by the polymerase chain reaction (PCR) method. Instrumental methods were used to detect the presence of fragrance allergens in cosmetic products. A method of quantifying these allergens using liquid chromatography was introduced and the measurement results were compared with the results of gas chromatography with mass detection. The proposed method of isolation and subsequent amplification of plant DNA is suitable for determining the authenticity of herbal ingredients in cosmetic products. The proposed HPLC method is a simple and inexpensive method suitable for determining the allergens present in the amount necessary to comply with EU analyte legislation and in the required sensitivity (except for hydroxycitronellal). Both methods are suitable to be used for the analysis of food products, too.
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Linguistic Analysis of podcast Zkouškový
Tesařová, Tereza ; Klabíková Rábová, Tereza (advisor) ; Šimková, Karolína (referee)
The bachelor's thesis "Linguistic Analysis of podcast Zkouškový" deals with a relatively young format of audio content that has recently become a popular form of shared content. Practically everyone has a podcast nowadays. The analysis will deal with a podcast series about life of young people. Just like the format in which the show was produced, the creators of Zkouškový are young people bringing new topics that are downplayed by society. The aim of this bachelor thesis is to present a comprehensive case study. To evaluate whether the topics presented are in line with the motivation behind the show. To evaluate whether the used words are appropriate to decribe the life of young people. The theoretical part of the thesis will anchor the thesis by using definitions, portraying the current podcast scene and introducing the programme under analysis. The analysis will be based on the principles of conversation analysis, focusing on how the characters are and what their behaviour is. It will analyze the podcast series linguistically by using a description of typical linguistic devices and excerpts from the podcast series. It will evaluate how the dominant themes are presented. In the conclusion of the bachelor thesis, we summarize the findings already established. We evaluate whether this type of audio...
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Authenticity on BeReal, the new social media app
Ouhrabková, Eliška ; Oukropec, Jindřich (advisor) ; Švecová, Martina (referee)
The topic of this bachelor thesis is the examination of authentic performance on the new social media site BeReal. The ambition of this thesis is to use qualitative methods to find out how selected respondents present themselves on this social network. The theoretical part of the thesis focuses on communication, social networks, self-presentation, authenticity and the introduction of the BeReal app. In the practical part, the research methodology is introduced to familiarize the reader with the research sample and research questions. It also presents the justification for the chosen qualitative methods, which in this thesis are semi-structured interviews and the digital tracing method attached to them. The methodology section is followed by the research section of the thesis. This is divided into subsections according to the research questions, which aim to help answer the main research question concerning the extent of user authenticity. The last chapter of the research is then a discussion of the results, which summarises the interview findings and links them to the theoretical knowledge from the first part of the thesis. The final section of the paper provides a brief summary of the results and suggestions for further investigation of the phenomenon.
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The Scar
Sláma, Matěj ; Nytra, Martin (referee) ; Mazanec, Martin (advisor)
Diploma thesis Scar is a short film about the fictional history of the found „monument“. In the animated film, I examine the context and meaning of the „monument“ in a virtual environment that I created based on visual documentation and mapping of specific places that I explored during my expeditions through the urban landscape of Brno and The Hague. These are mainly „lost“ spaces (also referred to as vague terrain or no man's land), which inspired me to create my own visual map of my urban explorations. The starting point of the work is personal lived experience, which I reflect by documenting places, creating 3D objects and manipulating 3D scans of „lost“ spaces with elements of computer games. In the resulting film, the viewer follows a guide–walk through a virtual environment where objective reality is intentionally blended with a fictional world built on the author's imagination.
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I Am The One Who's Gonna Steal Your Job
Ilič, Risto ; Houser, Milan (referee) ; Mazanec, Martin (advisor)
The diploma thesis I Am The One Who's Gonna Steal Your Job deals with the transitional period in the life of a young artist who dreams of career realization in his field. He thinks about how to achieve this when he realizes that even a less established painters can often sell their paintings for thousands of czech crowns, because paintings can be easily commodified and, as a result, placed in the interior. He thus decides to leave his area of practice and tries to get into the market with traditional artistic media with the help of artificial intelligence and marketing. He doesn't go far for visual inspiration, as he tries to recycle and distort photos (so-called visual smog) of a corner store on the other side of the street. The thesis deals with the issue of the emergence of artificial intelligence in the field of visual arts and with the eternal question of the ethics of the authorship of a work of art and its authenticity. It also deals with the question of the employment of art school graduates. Therefore, the work does not only provide a subversive analysis of the art market but also a reflection of the position of the artist participating in this market.
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