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Sintering of Char from Biomass
Hanus, Vlastimil ; Baláš, Marek (referee) ; Lisý, Martin (advisor)
: Hanus Vlastimil: Melting of ashes from biomass. Diploma Thesis Masters 5th year, academic year 2009/2010, the study group 5o / 2 FSI VUT Brno, Department of Energy, Department of Energy Engineering, May 2010th The project developed under the Energy Master field engineering design work by Melting of ash from biomass. Job description is to undertake research of methods for determining the characteristic temperatures of ash. Described are the properties of biofuels and their composition. Experimentally verify the individual methods and the influence of basic parameters on the temperature.
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Binders on the base alumina cements and their properties
Morávková, Jitka ; Cupák, Petr (referee) ; Burianová, Klára (advisor)
The Bachelor Thesis describes chemical and mineralogical structure, as well as alumina cements manufacture and some of their physical and techno-logical properties. It takes accelerators or plasticizers induced changes by search method. An illustrative laboratory measurement of mixture with accel-erator and plasticizer is complementary to theoretical knowledge in fluidity, air permeability and strength of mixture with aluminium silicate cement binder.
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Stability of vesicular systems using fluorescence spectroscopy techniques
Máčala, Jakub ; Venerová, Tereza (referee) ; Mravec, Filip (advisor)
This thesis is focused on possibility of studying stability and fusion of catanionic vesicles with Förster resonance energy transfer. The mainly used technique was Time-Correlated single photon counting. Firstly, excitation and emission spectra of chosen probes were measured and donor-acceptor pairs were suggested: 5-hexadecanoylaminofluorescein with Octadecyl Rhodamine B, Bodipy 493/503 with rhodamine or DiI, perylene with fluorescein, DiO with DiI. Then, time-resolved measurements of suggested pairs from environment of catanionic vesicles with different content of cholesterol were made in order to track the FRET associated with fusion of vesicles. It was found out, that it is not possible to use DiO as a donor because of it’s inefficient solubilisation into vesicles. It is also not possible for Bodipy to be used as a donor, because of it‘s excimer formation. In case of using fluorescein as a donor, it was found, that there is ongoing homo-fret between fluorescein molecules. Thanks to this, fusion was tracked by addition of unstained vesicles. It was also possible to track fusion in longer period of time. Also perylene-fluorescein pair was found to be capable of tracking the fusion, but with the exception of vesicles with content of cholesterol of 43 mol. %, tracking of fusion was possible only in short period of time.
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The optimization of melting as a decomposition process for material analysis.
Ptáček, Martin ; Koplík, Jan (referee) ; Šiler, Pavel (advisor)
Fusion is the process in which the sample particles are dissolved in the flux. This is used for the decomposition of substances which under normal conditions do not decompose in acid. For example, silicon, which is a substantial part of cement, is not degraded under standard conditions, so it is fused. Fusion may be in solution or in a pearl. Solution fusing is used for ICP-OES rather than XRF pearls. In addition to the pearl, XRF can also be measured in powder, but it is less accurate. This bachelor thesis deals with decomposition of standards and comparison of measured values with certified values. In the course of the work, samples of the standards were decomposed using several types of fluxes, measured at ICP-OES. The same standards were measured using XRF in the form of pearls. In addition to these two methods, elemental analysis of sulfur content was also performed. These methods were finally compared. From the measured data we can say that the best results are achieved with the ICP-OES method.
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Binocular vision
Němcová, Andrea ; Šanda, Jaroslav (referee) ; Fedra, Petr (advisor)
This bachelor thesis deals with the physiology of binocular vision for obtaining three-dimensional perception from two-dimensional images when using special glasses. It focuses on anatomy and physiology of the human eye and vision as inseparable parts of binocular vision, and on binocular vision as such. The work also mentions terms related to vision and describes the evolution of binocular vision during the human life. The important part of this thesis is the description of the emergence of three-dimensional perception from two two-dimensional pictures (stereogram). The thesis explains the principles of 3D projection methods, especially those which use active or passive glasses, and briefly describes the possibilities of 3D projection without glasses. The practical part includes a plan of capturing dynamic scenes where important parameters which affect the video capturing are described. The plan provides a description of a dynamic scene design with respect to the possibility of verification of human physiological parameters. Described scenes were captured by two identical cameras and edited in appropriate software. These videos were shown to a group of viewers whose task was to evaluate the videos from both subjective and objective points of view.
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Acoustic Scene Classification from Speech
Dobrotka, Matúš ; Glembek, Ondřej (referee) ; Matějka, Pavel (advisor)
The topic of this thesis is an audio recording classification with 15 different acoustic scene classes that represent common scenes and places where people are situated on a regular basis. The thesis describes 2 approaches based on GMM and i-vectors and a fusion of the both approaches. The score of the best GMM system which was evaluated on the evaluation dataset of the DCASE Challenge is 60.4%. The best i-vector system's score is 68.4%. The fusion of the GMM system and the best i-vector system achieves score of 69.3%, which would lead to the 20th place in the all systems ranking of the DCASE 2017 Challenge (among 98 submitted systems from all over the world).
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Emotion Detection from Speech
Popková, Anna ; Fér, Radek (referee) ; Matějka, Pavel (advisor)
This Bachelor Thesis deals with research in the field of emotion recognition mainly from speech and marginally from other modalities (video and physiological data). It closely describes the topology of the systems built specifically for the subject of this work. Moreover, it describes experiments leading to optimized pre-processing, regressor training and post-processing. Data used for these research origins from evaluation AV+EC 2015. Results of fusion systems producing the most precise prediction were sent to this evaluation. The Bottle-Neck features are newly tested and combined favorably with commonly used eGeMAPS features for the recognition of arousal. For valence, two kinds of video features are used. Muli-task system (recognizing both valence and arousal) using Bottle-Neck features produces competitive results and is only 13 % relatively behind the mentioned fusion system. This is especially appealing for applications where only audio is available.
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Studying the evolution of chromosome rearrangements in pipid frogs using in situ hybridization
Bergelová, Barbora ; Knytl, Martin (advisor) ; Johnson Pokorná, Martina (referee)
In African pipid frogs we can find at least eight polyploidization events in which a lot of levels of ploidy arose. This group includes diploid, tetraploid, octoploid, and/or dodecaploid species. Due to this great variability in ploidy levels, representatives of the genus Xenopus are excellent model organisms for studying the evolution of whole-genome duplications and chromosomal rearrangements. Currently, little information is known about chromosomal rearrangements within the Xenopus species, specifically, there are only two known cases of large-scale rearrangements. The first is a fusion of chromosomes 9 and 10 in the subgenus Xenopus and the other rearrangement is a non-reciprocal translocation occurring between chromosomes 2 and 9 in the subgenus Silurana. In this diploma thesis, we attempted to expand the knowledge about chromosomal rearrangements using the fluorescence in situ hybridization method. We confirmed the fusion of chromosomes 9 and 10 in other species of the Xenopus genus, such as X. pygmaeus, and proposed a hypothesis about the possibility that this fusion occurred in the common diploid ancestors of this subgenus. We also gained further insights into the origin of non-reciprocal translocation within the Silurana subgenus. This translocation was not detected in the species X....
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In vitro models for studying Syncytin-1-induced fusion of trophoblast cells
Jech, Lukáš ; Trejbalová, Kateřina (advisor) ; Zíková, Martina (referee)
Trophoblast cell fusion is essential for human placenta development. Apart from initiating blastocyst implantation, syncytialization is critical for optimal nutrient and gas exchange between mother and fo- etus. Multicellular syncytia called syncytiotrophoblast covers the surface of the branched structure of chorionic villi, which is in direct contact with maternal blood. Impairment of the syncytialization process leads to insufficient fetal nutrition and severe pregnancy complications. Syncytia formation is induced by the interaction of the surface glycoprotein of retrovital origin, Syncytin-1, with its receptor. Despite the significance of these processes, the details of cell fusion and trophoblast differentiation remain unk- nown. Furthermore, because of its uniqueness, the human placenta cannot be covered by animal models. As a result, research into human placental development, especially Syncytin-1-induced trophoblast cell fusion, is limited to in vitro trophoblast models. These models include primary trophoblast cell cultures and trophoblast cell lines, which can be obtained by immortalizing cell cultures or extracted from trophoblast tumours. Dedifferentiated trophoblast stem cell cultures were also established. The most recent approach, however, involves the direct reprogramming of dermal...
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