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Project Teaching in Teaching of Human Rights in Civics
Nováková, Lucie ; Zicha, Zbyněk (advisor) ; Dvořáková, Michaela (referee)
This diploma thesis deals in the theoretical part with comparing the Charter of Fundamental Rights and Freedoms of the Czech Republic and the Slovak Constitution with the Declaration of Human Rights in the sense of historical acceptance in the two countries under consideration. It includes a proposal for a educational project in this area (e.g. the creation of supporting worksheets). It deals with the topic of Human Rights in FEP for Elementary Education and specific SEPs of selected schools. The diploma thesis focuses on the preparation of a new Human Rights education project using selected effective methods. The proposal of this project will be based on the comparison of already existing educational projects.
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The institute of the citizenship of the Republic of Austria
Nováková, Lucie ; Jirásková, Věra (advisor) ; Mlsna, Petr (referee)
Thesis title: The institute of the citizenship of the Republic of Austria Citizenship is often defined as a status of a person, who is recognised under the law as being a legal member of a sovereign state. This status is usually connected with certain rights and privileges, along with corresponding duties, which belong to the citizens only. This master thesis aims to give a detailed analyse of the valid legislation of Austrian citizenship and to evaluate it critically. The main goal of the thesis is to answer the questions, whether the nowadays valid regulation is still actual and whether it would not be better to regulate certain issues in a different or more suitable way. In the introduction of this thesis the term citizenship and the related key concepts are explained. Mentioned are also the leading principles of the regulation of the Austrian citizenship, structured by their importance according to the Austrian legal literature. Introduced are also the sources of Austrian law - the significant international treaties and the regulation in the European Union and in the Austrian constitution. However, the main emphasis is put on the valid Austrian Citizenship Act, which contains large number of information related to this thesis. Described are also the ways of obtaining and losing the Austrian...
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Matrix effects in LC-MS analysis: occurrence, evaluation, and their elimination
Svoboda, Pavel ; Nováková, Lucie (advisor) ; Švec, František (referee) ; Havliš, Jan (referee)
Abstract Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Mgr. Pavel Svoboda Supervisor: Assoc. Prof. PharmDr. Lucie Nováková, Ph.D. Advisor: RNDr. Hana Vlčková, Ph.D. Title of Doctoral Thesis: Matrix effects in LC-MS analysis: occurrence, evaluation, and their elimination The dissertation thesis deals with the issue of matrix effects in analysis using liquid chromatography coupled to mass spectrometry (LC-MS). LC-MS represents a well-established, widely used, and powerful tool in the field of qualitative and quantitative analysis of a wide range of different compounds. Despite all the advantages offered by LC-MS such as sensitivity, selectivity, and robustness, it suffers from a major drawback represented by matrix effects. This undesirable phenomenon affects important parameters of LC-MS method such as accuracy, precision, linearity, limit of detection, and limit of quantification. Therefore, an evaluation of these effects should be involved in validation of every single LC-MS method and their source should be eliminated. The assessment is generally performed by qualitative and quantitative approaches such as post-extraction addition, post-column infusion, and comparison of slopes of calibration curves. The matrix effects can be reduced or...
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Evaluation of separation potential of stationary phases based on porous graphitic carbon
Guláš, Ondrej ; Nováková, Lucie (advisor) ; Šatínský, Dalibor (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Ondrej Guláš Supervisor: Assoc. Prof. PharmDr. Lucie Nováková, Ph.D. Title of Diploma Thesis: Evaluation of separation potential of stationary phases based on porous graphitic carbon. Stationary phases based on porous graphitic carbon (PGC) exhibit many interesting features. For example high mechanical resistance, stability across the entire pH range and excellent high temperature resistance. Moreover, they are significant due to their unique mechanism of retention. Despite all of these positive features and long existence on the market, their broader use in pharmaceutical analysis is still lacking. In our paper we have analyzed 11 groups of drugs, including active pharmaceutical ingredient and its potential impurities. Overall, 67 substances have been analyzed. Each separation on PGC has been optimized in order to achieve the best results. Changes in conditions of separation included testing of several mobile phases, gradient and isocratic elution and testing the effect of higher temperature. The use of gradient elution, with mobile phase consisting of acetonitrile and isopropanol in proportion 1:1 as organic modifier and 0,1% trifluoracetic acid as aqueous part has appeared as the most...
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Development of UHPSFC-PDA method for impurity profiling in active pharmaceutical ingredient atomoxetine
Roubíčková, Lucie ; Nováková, Lucie (advisor) ; Jáč, Pavel (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Bc. Lucie Roubíčková Supervisor: Assoc. Prof. PharmDr. Lucie Nováková, Ph.D. The title of Diploma Thesis: Development of UHPSFC-PDA method for impurity profiling in active pharmaceutical ingredient atomoxetine This diploma thesis deals with an optimization of UHPSFC method for determination of atomoxetine and its impurities mandelic acid, o-cresol, phenol, phenoxyatomoxetine, benzylatomoxetine and atomoxetine carbamate. Atomoxetine is used as centrally acting sympathomimetic agent for the treatment of hyperkinetic disorders such as Attention Deficit Hyperactivity Disorder (ADHD). Measurements were carried out on the UHPSFC system Aquity UPC2 with PDA detector and Torus Diol 1.7 µm (3.0 x 100 mm) column. ABPR pressure was optimized at 2000 psi and the column temperature at 40řC. Flow rate of mobile phase was 1.5 ml/min. Additional optimization parameters were the mobile phase composition, gradient elution conditions (initial composition of mobile phase, gradient slope, gradient time, gradient curves) and the effect of analysis time on the resolution of critical peak pairs. PDA detector parameters were examined, including comparison of data acquisition in 3 D and 2 D mode, selection of detection...
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Characterization of separation systems for determination of enantiomers
Geryk, Radim ; Tesařová, Eva (advisor) ; Ševčík, Juraj (referee) ; Nováková, Lucie (referee)
(EN) The dissertation thesis is focused on the research and characterization of retention and enantiorecognition mechanisms of chiral stationary phases based on derivatized polysaccharides. The separation systems with a variety of modern stationary phases (both achiral and chiral) were characterized in detail to provide a comprehensive view of the interactions participating in the separation process. The study of the retention/separation behavior significantly facilitates the development and the optimization of new enantioselective methods for a wide variety of compounds. The work deals with the comparison of enantioselective performance of polysaccharide-based chiral stationary phases. The objectives are to show the differences of separation behavior among these chiral stationary phases, as they differ by the nature of the polysaccharide backbone (amylose versus cellulose), by binding of chiral polymer to silica support (coated versus immobilized stationary phase) and by the phenyl moiety in the reversed and normal phase HPLC. In both separation modes amylose-based chiral stationary phases exhibited higher enantioselectivity, especially for acidic and bifunctional analytes. Chiral stationary phases based on derivatized cellulose showed higher enantiodiscrimination potential for basic analytes....
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Optimization of SFC method for chiral screening
Jakubec, Pavel ; Nováková, Lucie (advisor) ; Chocholouš, Petr (referee)
3 ABSTRACT Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Pavel Jakubec Supervisor: Assoc. Prof. PharmDr. Lucie Nováková, Ph.D. Title of Diploma Thesis: Optimization of SFC method for chiral screening Method for effective chiral screening was developed for 37 chiral pairs of compounds of different structures and acid-base properties. Measurement was performed on SFC system Acquity UPC2 with PDA detector, using chiral polysacharides columns with particles 2.5 μm or 3 μm. Mobile phase flow was set to 2.0 ml/min due to the maximum pressure limits of most of the tested columns. Gradient elution with column temperature set to 40řC and ABPR pressure of 12 MPa was used. For primary screening 10 mobile phases were chosen, from which 2 best were used for all of the tested columns. An equivalence of columns with the same chiral selectors phase from different manufacturers was not confirmed. Considering the complexity of chiral recognition mechanisms general rules for the choice of the columns or mobile phase could not been clearly defined. Chiralcel OD-3 with IPA + 0.1% TFA,DEA was column with the most separated enantiomers. Chiralcel OD-3 with MeOH + 0.1% TFA,DEA was the best column considering overall percentual scale including the resolution and...
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Two-faced Garden: Different approaches to The Cement Garden
Nováková, Lucie ; Kupcová, Helena (advisor) ; Sládek, Miloš (referee)
The aim of my bachelor thesis is to analyze the first work of a contemporary British author Ian McEwan, The Cement Garden and to present its wide influence on art. To achieve my intention, I have focused on analyzing the play performed by Švandovo divadlo. The analysis is based on crucial events, such as the death of the father, the perish of the mother and her burial and final incestuous scene. Each moment has been closely examined so that one may understand the process of adaptation. The explanation of an adaptation process has been provided to support my claims. An opening chapter delves on the life and work of Ian McEwan, mentioning The Cement Garden which historical context is provided and on the remark regarding an evolutional process of literary work which is concerned with child protagonists. Author's inspirations and motives are also mentioned. Second chapter not only closely analyzes the novel and the crucial events, but also focuses on formal requirements, such as the narrator or the setting. Meanwhile the next chapter considers the theater adaptation and its accomplishments and downfalls. Brief explanation of possible adjustments is provided as is the difference between the prosaic text and the script. Final part is dedicated to the movie adaptation by Andrew Birkin. Unlike the previous...
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Optimalization and validation of SPE method for determination of quercetin and its 9 metabolites in biological material
Chrenková, Lucia ; Nováková, Lucie (advisor) ; Vlčková, Hana (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of analytical chemistry Candidate: Lucia Chrenková Supervisor: doc. PharmDr. Lucie Nováková, Ph.D. Title of Graduation Thesis: Optimalization and validation of SPE method for the determination of quercetin and its 9 metabolites in biological material. The aim of this study was to develop and validate a new extraction method for the preparation of biological samples for the determination of quercetin and its 9 metabolites: phloroglucinol, 3,4-dihydroxyphenylacetic acid, homovanilic acid, 3-hydroxyphenylacetic acid, 3-(3-hydroxyphenyl)propionic acid, rutin, quercetin-3-glucuronide, tamarixetin and isorhamnetin. Due to the best retention and subsequent elution of all tested analytes the mixed mode (polymeric ion exchanging) cartridge MAX was chosen. The mixture of methanol and 0.5 % trifluoracetic acid in water (95/5) was chosen as optimal solvent for elution. The combination of 0.01M ammonium formate buffer pH 5.0 and 1% methanol in buffer pH 5.0 were chosen as washing solvent. The determination of quercetin and its 9 metabolites was performed using UHPLC- MS/MS. The best selectivity between the critical pair of analytes with the same molecular weight (tamarixetin and isorhamnetin) was achieved using BEH Shield RP18 column and...
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