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Regulation of integrative and complementary medicine
Tesařová, Eva ; Pokladníková, Jitka (advisor) ; Marešová, Helena (referee)
Regulation of integrative and complementary medicine Student: Eva Škulková Tutor: PharmDr. Jitka Pokladníková, Ph.D. Dept. of Social and Clinical Pharmacy, Faculty of Pharmacy in Hradec Králové, Charles University, the Czech Republic Background: The prevalence of complementary and alternative medicine (CAM) is high 76% and it is rising. In the Czech Republic, there are no legislation or general standards that would ensure uniform requirements for providing CAM. At present, there is a lack of comprehensive understanding of how CAM methods are provided. Aim: To determine the level of regulation of the most commonly used CAM methods in the Czech Republic. Methods: The level of regulation has been evaluated according to regulatory criteria based on requirements for healthcare professionals. The list of the researched methods is based on foreign studies and at the same time specific CAM methods for the Czech Republic were added. Specific methods were selected by the expert group. The data was obtained by searching the Internet using The Google. The obtained data was processed in MS Excel using descriptive statistics. Results: Altogether, 98 CAM methods were searched. Of these, 28 methods were excluded from the evaluation, 70 methods were evaluated according to the criteria, and 2 methods were double...
Chromatograhic characterization of chiral and achiral separation systems
Kučerová, Gabriela ; Tesařová, Eva (advisor) ; Čabala, Radomír (referee) ; Ševčík, Juraj (referee)
Dissertation thesis is a 5-publications' collection concerning characterization and application potential of cyclodextrins, polysaccharides and macrocyclic antibiotics based chiral stationary phases. The effects of stationary phase and mobile phase are studied. This approach ensures the complex insight into separation systems studied. Systems with different nature of chiral selector were studied by HPLC. Namely, macrocyclic antibiotics and derivatized polysaccharides were used for experiments. Former ones provided better results for enantioseparation of non-coded amino acids than latter ones. Dynamic coating procedure was used for preparation of a new chiral stationary phase. Characterization of new cationic cyclodextrin based chiral stationary phase was performed. Linear free energy relationship method was used for characterization of two different separation systems, i.e. newly prepared stationary phase and commercially available stationary phase. Based on results obtained, newly prepared stationary phase showed better results for separation of different achiral groups of analysts. New stationary phase prepared by dynamic coating was compared with chromatographic system, in which the chiral selector was used as a mobile phase additive. The chiral selector used for the two different approaches was...
Separation and characterizasion of stereoisomers of phosphinic pseudopeptides and serine by capillary electrophoresis
Koval, Dušan ; Jelínek, Ivan (advisor) ; Feltl, Ladislav (referee) ; Tesařová, Eva (referee) ; Stránský, Zdeněk (referee)
CoNcLUstoNs This work demonstrates that capillary electrophoresis is a suitable method for analysis and physico-chemical characterization of biomolecules. lt was exemplified by px" determination of a series of nove| phosphinic pseudopeptides and by separation oÍ stereoisomers of the phosphinic pseudopeptides and serine' A self-consistent procedure for pK" determination by CZE was carried out' ln particular' various background electro|ýes Were tested in the acidic region at pH < 2. A set of background eIectro|ýes Was eVa|uated for reIiab|e determination of eIectrophoretic mobiIities in the 1.S - 12pH interval. The temperature of the solution inside the separation capillary was measured by a simplified approach, thus enabling precise determination of the electrophoretic mobilities' Additionally, a throughput of the CZE method for the pK" determination was substantially improved, while high separation efficiency and precision of the electrophoretic mobilities was retained. The method benefits from adjustable velocity of e|ectroosmotic flow, achieved by modiÍication of the capi||ary inner surface, and efficient determination of electroosmotic mobility. Further, a study was performed which exp|ored the validity of onsager mode| for theoÍetica| description of concentration dependence of the e|ectÍophoretic...

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