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Case Study of Responsible Together Campaign
Nováková, Lucie ; Pavlík, Petr (advisor) ; Moree, Dana (referee)
The aim of this thesis is to analyze a media campaign called Responsible Together, which is focused on decrease of violence against women. Campaign Responsible Together is created by non-profit organizations that are part of civil society. This thesis brings a description of the creative process of the campaign, it's target group, and communication mix. One part of this thesis also introduces the specifics of marketing in the non-profit field. A case study was selected as a research design because it allows combining more than one method of collecting data. These methods were document analysis, analysis of graphics, and participatory observation.
A study of separation conditions and stability of selected phenolic compounds
Jandorová, Zuzana ; Nováková, Lucie (advisor) ; Kočová Vlčková, Hana (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Zuzana Jandorová Supervisor: Prof. PharmDr. Lucie Nováková, Ph.D. Title of the diploma thesis: A study of separation conditions and stability of selected phenolic compounds The goal of this diploma thesis was to determine the most suitable separation conditions for the analysis of ten phenolic compounds by the ultra-high performance liquid chromatography with PDA detection. The thesis is focused mainly on the separation of critical pair tamarixetin and isorhamnetin. Another goal was to determine the short-term stability of all tested compounds for 24 hours. Analyses were performed using the ACQUITY UPLC chromatographic system with PDA detector at a wavelength of 275 nm. The first step in the study of separation conditions was the screening of 17 columns from several different manufacturers. Tested columns included ACE Excel C18, BEH C18, CSH C18, Arion Plus C18, Ascentis Express C18, Atlantis Premier BEH C18 AX, BEH Shield RP18, Cortecs Shield RP18, Ascentis Express RP-Amide, BEH Phenyl, CSH Phenyl-Hexyl, Kinetex Biphenyl, ACE Excel C18-AR, ACE Excel C18-PFP, CSH Fluoro-Phenyl, Ascentis Express F5, and Kinetex PFP. During the screening, 3 organic modifiers (acetonitrile, methanol and acetonitrile...
Optimization of conditions for qualitative and quantitative analysis of farnesol and tyrosol using UPLC-MS/MS.
Greguš, Petr ; Nováková, Lucie (advisor) ; Šatínský, Dalibor (referee)
Direct activation of C-H bond in tetrazoles Richard Lubojacký, 5th grade Supervisor: Prof. RNDr. Milan Pour, Ph.D. Department of Inorganic and Organic Chemistry, Faculty of Pharmacy in Hradec Králové Within the framework of this diploma thesis, direct arylation and alkenylation of 1- substituted tetrazoles at C5 was developed and optimized. An economical preparation of a range of 1,5-disubstituted tetrazoles was thus made possible. In these reactions, Pd-catalysis in the presence of CuI and Cs2CO3 was found as optimal. Contrary to similar reactions of imidazoles and purines, it was necessary to incorporate phosphine ligands in order to stabilize Pd - intermediates and prevent them from a premature decomposition into cyanamides. A library of 1,5-disubstituted tetrazoles with very promising yields has been prepared using this method.
Evaluation of properties of new HILIC stationary phases for pteridins using UHPLC-FD method
Kaufmannová, Iva ; Nováková, Lucie (advisor) ; Šatínský, Dalibor (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of analytical chemistry Candidate: Iva Kaufmannová Supervisor: PharmDr. Lucie Nováková, Ph.D. Title of Doctoral Thesis: Evaluation of properties of new HILIC stationary phases for pteridins using UHPLC-FD method The subject of this thesis was to evaluate the characteristics of three new types of HILIC stationary phases for the identification and quantification of biologically active substances biopterin, neopterin, dihydrobiopterin and dihydroneopterin by UHPLC with fluorescence detection. Neopterin is used as a marker of immune system activation and inflammatory diseases. Its early detection in urine or plasma may indicate a pathological immune activity. Elevated concentration of neopterin is described in viral or bacterial diseases, in autoimmune diseases in HIV infection or in malignant tumors. 7,8 - dihydroneopterin is able to prevent proteins and lipoproteins from oxidative damage. Hydrophilic Interaction Liquid Chromatography (HILIC) is a chromatographic method that can be used to improve retention of very polar compounds. It uses a polar stationary phase and mobile phase containing a certain amount of water and polar solvents. UHPLC is the most advanced separation technology to develop a pressure of about 100 MPa....
Morphological variation of selected European Soricinae shrews
Nováková, Lucie
Soricinae shrews are one of the most common and abundant mammalian group in Holarctic Region. The uniform phenotype and hidden way of life make the research on this group challenging. Basic rules that drive their phenotype and morphological variation still remain unclear. The aim of this dissertation is to clarify the selected aspects of morphological variation in three European Soricinae species. The first part of this thesis is aimed on age and sex skull variation in a single population of the common shrew (Sorex araneus). We found significant sexual dimorphism and differences between age groups. Based on this study we selected cranial variables suitable for following studies. The second part of the dissertation project was aimed on geographic variation of the common shrew in the area between the Baltic and the Adriatic sea. We found correlation between size of shrews and latitude, longitude as well as altitude. Size was also correlated with selected climatic variables. The third part of the project was focused on a description of Dehnel's phenomenon and its geographic pattern. We found that decrease and regrowth of the braincase is separately correlated with different climatic variables. Our results suggest that different evolutionary processes and pressures are involved with two phases of...
Development of method for the determination of entecavir in biological materials using UHPLC-MS/MS
Gottvald, Tomáš ; Nováková, Lucie (advisor) ; Šatínský, Dalibor (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Tomáš Gottvald Supervisor: Doc. PharmDr. Lucie Nováková, Ph.D. Title of thesis: Development of method for the determination of entecavir in biological materials using UHPLC-MS/MS Entecavir is a synthetic guanosine nucleoside that plays an important role in treatment of chronical hepatitis B virus infection. This work was focused on development of highly sensitive method to determine entecavir concentrations in rat urine by ultra-high performance liquid chromatography-tandem mass spectrometry. Entecavir C2 13 N15 , stable isotopically labeled internal standard, was used for quantitation. Hydrophilic interaction chromatography seems to be a suitable technique for retention and separation of polar compounds present in analyzed sample. One of the main goals was to confirm this fact by comparison with commonly used reverse phase chromatography mode (RP-UHPLC) on BEH C18 stationary phase. Conditions for RP-UHPLC were optimized as follows: mobile phase composed of acetonitrile/0.01 % formic acid (4:96). The HILIC conditions on BEH Amide stationary phase were optimized using isocratic elution with mobile phase composed of acetonitrile/5 mM ammonium acetate pH 4.0 (75:25). HILIC method provided much...
The development of HILIC method for UHPLC-MS/MS determination of pteridins, a comparison of selectivity of various stationary phases.
Jánská, Radka ; Nováková, Lucie (advisor) ; Chocholouš, Petr (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Radka Jánská Supervisor: PharmDr. Lucie Nováková, Ph.D. Title of Doctoral Thesis: The development of HILIC method for UHPLC-MS/MS determination of pteridins, a comparison of selectivity of various stationary phases This graduation thesis was dealing with the development of HILIC method for the identification and quantification of biologically active substances neopterin, biopterin, 7,8- dihydroneopterin and 7,8-dihydrobiopterin by ultra-high performance liquid chromatography coupled to the mass spectrometry detector of triple quadrupole type. Three chromatographic columns (BEH Glycan, BEH Amide and BEH HILIC) were tested. Several mobile phases and their influence on the separation of target analytes were tested. Mobile phase consisted of aqueous component (acetic and formic acid, ammonium formate and acetate and ammonim hydroxide of low concentration) and acetonitrile. On the chromatographic column BEH Glycan following best mobile phases were evaluated: 1mM ammonium acetate pH= 3.8 with acetonitrile in the ratio 30:70 and 1mM ammonium acetate pH= 6.8 with acetonitrile in the ratio 28:72. Two mobile phases composed of 1mM ammonium acetate pH= 4.8 with acetonitrile in the ratio 23:77 and...
Brittle tectonics in the NE Bohemian Massif as related to recent tectonic movements indicated by GPS measurement
Nováková, Lucie
North-eastern part of the Bohemian Massif is characterised by many NW-SE striking faults. The Sudetic Marginal Fault Zone (SMFZ) and Hronov - Poříčí Fault Zone (HPFZ) represent the major seismoactive dislocations in this area. Field structural investigations, including fault-slip data collection were carried out on a number of natural outcrops and quarries with the aim of establishing a robust and field-constrained model for the local brittle structural evolution of the studied areas. Almost 5000 faults and fractures have been measured and studied in 116 localities. Two principle sets of faults within the SMFZ are oriented in the N-S and W-E directions. The faults are mainly dipping under 80-90ř. The lineations found on the fault planes are mainly trending to the SW and W. The kinematic frequent analysis was performed due to the distribution of the fault types in the orientations. The faults were divided into the different tectonic phases based on their origin or reactivation and their relative age using the calculation of paleostress analysis. The paleostress analysis of the fault-slip data within the SMFZ resulted identification of six tectonic phases from the youngest to the oldest: strike-slip regime with maximum compression σ1 in the NNW-SSE direction, compressional regime with σ1 in the...
Development of dispersive liquid-liquid microextraction coupled with capillary liquid chromatography for determination of fipronil and its two metabolites
Jirková, Tereza ; Nováková, Lucie (advisor) ; Horstkotte, Burkhard (referee)
1 ABSTRACT Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Tereza Jirková Supervisors: Dr. Francisco J. Lara prof. PharmDr. Lucie Nováková, Ph.D Title: Development of dispersive liquid-liquid microextraction coupled with capillary liquid chromatography for determination of fipronil and its two metabolites Fipronil is a phenylpyrazolic insecticide that is prohibited for use in food-producing animals. This diploma thesis proposes a green analytical methodology for the study of residual fipronil and its two degradation products, fipronil sulfone and fipronil sulfide. For their determination, a method of capillary liquid chromatography with UV detection was developed and validated. Correlation coefficients above 0.998 confirm good linearity and the study of repeatability with values not exceeding 2.9% RSD shows high precision of this method. For the dispersive liquid-liquid microextraction method used for sample preparation, the dispersing and extracting solvents were optimized. The results show that the highest recoveries 82% - 92% were achieved with dibromomethane as an extractant and acetonitrile as a dispersant. All the experiments were carried out with standard solutions and in further studies this methodology should be transferred to egg or other...
Development of UHPLC-MS/MS method for analysis of selected antivirotics in HILIC and RP mode
Fical, Luboš ; Nováková, Lucie (advisor) ; Lhotská, Ivona (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Luboš Fical Supervisor: Prof. PharmDr. Lucie Nováková, Ph.D. Title of the diploma thesis: Development of UHPLC-MS/MS method for analysis of selected antivirotics in HILIC and RP mode The goal of this diploma thesis is development and optimization of methods for analysis of selected antivirals and comparison of suitability of RP and HILIC modes for this application. Targeted antivirals are used in treatment of HIV and hepatitis B and/or C. LC analyses were carried out on the ACQUITY UPLC I-Class PLUS system with triple quadrupole MS detector. Initially MS/MS method was optimized. In RP mode total of 6 stationary phases (BEH Shield RP18, BEH Phenyl, BEH C18, CSH Fluoro-phenyl, CSH Phenyl-hexyl, and CSH C18) and 4 mobile phases (ACN in gradient elution with aqueous component: formic acid, acetic acid, ammonium formate pH 3, ammonium acetate pH 9) were tested. Separation conditions were as follow: temperature 40řC, flow rate 0.3 mL/min, gradient elution from 5 % ACN to 98 % ACN within 5 minutes with isocratic step at the end of analysis. In HILIC mode 10 stationary phases (BEH Amide, BEH HILIC, Cortecs HILIC, Ascentis Express HILIC, Ascentis Express OH5, Syncronis HILIC, Kinetex HILIC, Luna HILIC,...

National Repository of Grey Literature : 155 records found   1 - 10nextend  jump to record:
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31 NOVÁKOVÁ, Lenka
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