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Family House with an establishment
Flousek, David ; Pospíšil, Tomáš (referee) ; Čuprová, Danuše (advisor)
The theme of bachelor´s thesis is new-building family house with establishment in Machov. The building is set in a slighty sloping terrain. It has two overground floors and basement. The building is designed of system Porotherm P+D and ceiling construction of system Porotherm ceiling. The roof strukture is a flat roof.
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Controllers design for plants with small time constants
Rozmahel, Ivan ; Rojka, Aleš (referee) ; Davidová, Olga (advisor)
The main reason of this project is to set up and to propose continous controllers for process with small time responses. The regulated circuits with different type of regulators and its different settings were proposed in this project. These parameters were set up with method synthesis. These calculation were compared and evaculated as regards to control accurancy and quality.
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Transport of selected metals from the soil to the plants
Šilerová, Iva ; Sommer, Lumír (referee) ; Dočekalová, Hana (advisor)
This bachelor thesis focuses on the soil, contamination of selected heavy metals and transport of heavy metals from soil to plants. The entry of heavy metals, their reactions in the soil and their transport into the plant material are described here. Lead, cadmium and copper have been selected as the studied toxic metals. There are also described methods of soil sampling, sample preparation for analysis and determination of heavy metals in the sample.
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Renewable energy sources: are they always contributive to the environment?
Pelz, Václav ; Jašková, Jana (referee) ; Baumgartnerová, Alena (advisor)
Semestrální práce se zabývá obnovitelnými zdroji energie a jejich využitím. Snaží se přiblížit i laickému čtenáři základní principy fungování obnovitelných i neobnovitelných zdrojů a výrobu elektřiny s tím spojenou. Snaží se zároveň poukázat na mnoho otázek a problémů s obnovitelnými zdroji se kterými se v dnešní době potkáváme, jelikož poptávka po elektřině stále roste, a přírodní neobnovitelné zdroje jako je ropa či uhlí pomalu ale jistě mizí. Nastiňuje základní problémy s obnovitelnými zdroji a uvádí klady a zápory jejich využití i do budoucna.
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The role of small RNAs in transgenerational plant stress memory
Jaklová, Veronika ; Marková, Hana (advisor) ; Lipavská, Helena (referee)
Plants are constantly affected by various abiotic and biotic stresses, which cause a whole range of reactions.The resultcanbe increasedplantresistance tovariousstressfactorssuchasherbivoryattack or lack of water. Additionally, this resistance can also be passed on to subsequent generations through epigeneticmechanisms.Small RNAsservingas signaling moleculesof the plant's rapid response tostress can play a large part in the formationof intergenerationalandmultigenerationalstressmemory.MiRNAs are mainly regulators of gene expression,throughtheirinhibitory and degradative activitiesthey control the transcription of genes and the translation of a large number of proteins. SiRNAs could participate in the transfer of transcriptional memory through the mechanism of RNA-directed DNA methylation (RdDM). DNA methylation and histone modifications together act as chromatin marks that can be epigeneticallytransferredtosubsequentgenerations.Basedonthis,plants derivedfromstressedparent plants show large changes in gene expression compared to plants with non-stressed parents. These changesthenpersistfor varyinglengthsof time,dependingon whethergene expressionisagain induced bythe stressfactoror not.Interestinunderstandingthe mechanismsof transgenerationalstressmemory has recently grown considerably, and this...
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USE OF COMPLEX EXTRACTS FROM PLANT AND MICROORGANISMS IN FOODS AND COSMETICS
Uhlířová, Renata ; Kovalčík, Adriána (referee) ; Vorlová, Lenka (referee) ; Márová, Ivana (advisor)
The presented doctoral thesis is focused on optimalization of preparation of extracts of various origin (plant and microbial). The influence of extraction agent, disintegration of microbial cells as well as special techniques for enhancement of extraction process effectiveness (ultrasound and microwaves) were monitored. As part of the characterization, considering the source material, the composition of the prepared extracts was analyzed both spectrophotometrically and using liquid and gas chromatography. The most suitable extraction procedure varies depending on the type of material. For herbal samples, the highest content of active substances was achieved when the extraction facilitated by microwaves was used. Contrarily, the optimal method for cyanobacteria is the traditional extraction method, which is carried out for 24 hours at laboratory temperature. The most suitable method for extracting the active substances from yeast was a combination of DMSO, ethanol and hexane. Further, the effect of extracts on microbial cultures of bacteria (E. coli, S. epidermidis, C. acnes) and yeast (C. glabrata) was observed. For cytotoxicity evaluation MTT and LDH assays were performed on HaCaT and Caco2 human cell lines. The antimicrobial tests have demonstrated the antimicrobial, antibacterial and antifungal effect of a numerous extracts, especially the essential oils of Lavandula angustifolia and Mentha piperita. On the other hand, the cytotoxicity tests showed that the most of the extracts were safe for the use in the food industry and cosmetics within a range of up to 10% by volume. To improve stability, the prepared extracts were enclosed into liposomal particles enriched with PHB polymer (0–100%). Using a ZeteSizer NanoZS device, the particles' size, polydispersity index, and colloidal stability were assessed. The cryoTEM was used to examine the particle´s morphology. Liposomes and liposomes with the addition of 20 % PHB (PHB20) were evaluated as the most suitable particles for encapsulation of the prepared extracts. Excellent stability and high encapsulation efficiency were displayed by the produced particles. Genotoxicity testing with SOS chromotest on the model organism E. coli did not reveal any genotoxic effects. As an alternative method to achieve stabilization of active substances, nanofibers from PHB were prepared by three different techniques and gelatin nanofibers by electrospinning. The fibers were monitored for their diameter, uniformity, distribution on the collector using SEM and the gradual release of active substances from the fiber structure. The evaluations revealed that the forcespinning and electrospinning techniques were the most appropriate for further investigation. A continuous release of active ingredients was achieved by incorporating extracts into nanofibers. Several applications of prepared materials in the food industry and cosmetics were designed and developed as part of this work. The base emulsion's stability and general qualities were improved when green coffee and carotenogenic yeast extracts were added. The usage of emulsions containing coffee and yeast extracts would be suitable as sunscreens, and after the addition of Spirulina extract, as day or night anti-wrinkle creams. Energy drinks of the new type based on coffee with the addition of encapsulated plant extracts enable gradual release of caffeine and antioxidants during digestion.
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Coalescence
Lubovská, Karolína ; Příhoda, Jiří (referee) ; Ambrůz, Jan (advisor)
The theme for my bachelor's thesis is the landscape surrounding a south Moravian village called Bavory. To put this environment into context, I was looking into its historical and geographical background. All of my efforts were focused on one section of my family's property. The property consists of vineyards and a dried-up spring. In one part of my work, I observe the everyday activity around this spring, and in the second part of my work, I focused on making an installation out of dead grapevines.
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Generative growth
Zelený, Tomáš ; Vaněk, Vojtěch (referee) ; Gabriel, Michal (advisor)
The aim of this thesis is to emphasize the aesthetics of the support and also their functionality when placing objects into grown structures. Those objects seem fragile thanks to their chromaticity, but at the same time they need to be strong enough to hold the mass of diverse objects, such as piece of wood or stone. The aim of this thesis is so the scaffolding has its use in a real world. Scaffolding, that is now existing in the digital world, will pervade into the real world.
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