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Firm culture and its influence on motivation of firm's workers.
Zika, Jiří ; Kuchař, Pavel (advisor) ; Tuček, Milan (referee)
Bc. Jiří Zika Abstract The Diploma Thesis deals with the subject of firm culture and its influence on the motivation. It is focused especially on the analysis of firm culture and on the influence of firm culture on motivation. In this time is firm culture relatively well established field, and so this work is trying to point out the major approaches of firm culture. It analyses the known theories of motivation and then it deals with the links of firm culture and motivation. The influence of firm culture on motivation of workers working on business license is examined in the company, which deals with selling of real estates. This influence is being described especially by the influence of the elements of firm culture on the complete motivation and on different aspects of motivation as well. This work in its final form brings the reader an integrated view on firm culture, motivation and their interrelationship. It is focused especially on the influence of firm culture on motivation. The results of this work bring the information, that even under the conditions which are not favourable for firm culture it is possible to work relatively well on firm culture and its enforcement across the firm. These results go out from the combination of quantitative and qualitative methods. The research was based on the...
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Effect of climbing ability on energy expenditure of climbing
Kodejška, Jan ; Baláš, Jiří (advisor) ; Malý, Tomáš (referee)
The aim of the study was to evaluate the relation between climbing performance and energy demands while climbing through a specific submaximal test. The sample consisted of twenty-six climbers (age 26,8 ± 3,3 years, weight 70,6 ± 6,2 kg, height 177,6 ± 6,5 cm). Climbers were deliberately chosen so that their performance covers as much scale difficulty of UIAA (Union Internationale des Associations d'Alpinisme) as possible. The performance was given by the climbers currently the highest ascent in the style of RP (Red Point). Performance of these climbers ranged between 4 to 10 degrees UIAA. Climbers were subjected to submaximal climbing test, which lasted three minutes in one slope. They moved at a constant speed of 25 movements. min-1 on climbing route, already known in advance, the slope after 3 minutes changed from 90 ř to 105 ř. VO2 (oxygen consumption), SF (heart rate) and VE (minute ventilation) significantly correlated with climbing performance of RP (90ř, VO2, r = - 0,82; SF, r = -0,66, VE, r = - 0,77; 105ř, VO2, r = - 0,84; SF, r = -0,78; VE, r = - 0,80 ). Respiratory rate during submaximal climb was about 25 breaths. min-1, which refers to a link with a climbing speed. VO2 during submaximal climbing can be used to evaluate the economy of movement. Keywords sport climbing, submaximal test,...
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Contamination of powdered infant formula by Enterobacter sakazakii and other pathogens
Selmer, Arne ; Schneidrová, Dagmar (advisor)
Contamination of powdered infant formula by Enterobacter sakazakii and other pathogens will be considered in the following text with a description of the bacteria and their route of contamination as well as their pathological sequels together with preventive measures. E.sakazakii is a gram negative rod within the family Enterobacteriaceae. E. sakazakii in powdered infant formula has been implicated in outbreaks with sepsis, and infections of the central nervous system causing meningitis, cysts, brain abscess and necrotizing enterocolitis. Mortality has been reported to be as high as 50% but has decreased to less than 20% in the recent years. For those who survive subsequent developmental delay and hydrocephalus is a well recognized sequels. Enterobacter species are biochemically similar to Klebsiella, but unlike Klebsiella, it has has been found to be more resistant to osmotic and dry stress. Although exact virulence mechanisms are unknown, it is known that a small percentage of E.sakazakii cells can survive for extended periods in dehydrated powdered infant formula. While the organism has been detected in different types of food, only powdered infant formula has been linked to outbreaks of diseases. Disease caused by E. sakazakii in infants has been associated with the consumption of commercially prepared...
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Comparison of the rain rate measurements at different localities from the microwave attenuation viewpoint
Fišer, Ondřej
The rain rate distribution is the input data used in models predicting the rain attenuation distribution on microwave links. Some methods partially mitigating the rain attenuation are used in practice. The well known "site diversity" method is described in this contribution. The attenuation is obtained from the rain rates which are converted into the attenuation using the Assis-Einloft model. The rain rates are measured on 4 sites in the Czech Republic at distances from 8 to 150 km. It is shown that the site diversity gain is big (about 10 dB at 0.01% exceedance level at 12 GHz). The gain is increasing with the increasing distance (as expected) and is also a function of the azimuth of 2 sites. The north-south sites position has lower gain in comparison with the west-east position: it is due to the prevailing frontal motion from west to east in our country.
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Modulation of nociceptive synaptic transmission in the spinal cord dorsal horn
Špicarová, Diana ; Paleček, Jiří (advisor) ; Vlachová, Viktorie (referee) ; Rokyta, Richard (referee)
7 ABSTRACT Pathological pain states linked to several diseases or tissue damage are often associated with increased sensitivity to stimuli. The main underlying mechanisms of this hypersensitivity are peripheral sensitization of nociceptors and central sensitization in the spinal cord. One of the crucial processes of central sensitization is the modulation of synaptic transmission at the dorsal horn of the spinal cord. Studies included in my doctoral thesis investigate the possibilities of regulation of synaptic strength by cytokine TNFα, insulin and TRPV1 receptors agonist N-oleoyldopamine (OLDA). These three compounds are synthesized in the CNS, while TNFα is produced in the spinal cord notably during neuropathy. TNFα and insulin have a potential to modulate synaptic transmission. Endogenous TRPV1 receptors agonist OLDA can activate spinal TRPV1 receptors, which are highly expressed on central endings of nociceptive dorsal root ganglion (DRG) neurons. TRPV1 receptors are known as integrators of nociceptive stimuli particularly from the studies of peripheral receptors on nociceptors, which could be sensitized by inflammatory mediators and activated by temperature increase or decrease pH that is unlike in the spinal cord. In our experiments miniature excitatory postsynaptic currents (mEPSCs) or evoked EPSCs...
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The people of High Atlas (analysis on socio-cultural situation)
Konopíková, Michaela ; Soukup, Martin (referee) ; Soukup, Václav (advisor)
The core subject of this thesis deals with a theme of sociocultural change among the Ait Mizane, a small Berber community in the Moroccan High Atlas mountains. Living in a remote region with typical harsh climate they were throughout history known as free people who were never subordinated to any kind of central rule irrespective of them being Romans, Spaniards or Arabs. This long period of considerable isolation slowly ended with the advent of French rule, who took over the country proclaiming it as their colony and fitting the local reality within the frame of colonial admistration. But more dramatic shifts were yet to come. Silk-like fibres of globalization are nowadays entering this area forming a firm web that spreads all around. Ait Mizane people, Berber speaking farmers and herders occupy an environment they have shaped for centuries; There is a strong sense of identity which is very much linked to the cultural environment mastered by generations of their predecessors. The relationships they maintained with other mountain tribes as well as with city dwellers surpassed the test of time and fit the context of Muslim cultural milieu. Ancient strategies and traditions are still dominant ones and still determine the peoples' live with great power, but at the same time innovation occurs with an intensity...
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Stability studies of monoclonal antibody nimotuzumab modified with chelator DOTA radiolabelled with lutetium-177 ([177Lu]Lu-hR3(p-SCN-Bn)DOTA)
Petřík, Michal ; Lázníčková, Alice (advisor) ; Kubíček, Vladimír (referee)
The higher expressivity of the gene for the receptor of epidermal growth factor (EGF) and consequently higher number of receptors (EGFR) on the surface of cells is closely linked with the tumour proliferation of these cells and with poor prognosis for patients. For such reason the EGFR became one of the main objectives in the therapy of tumours. Nimotuzumab (also known as h-R3) is humanized monoclonal antibody which blocks receptor for epidermal growth factor. The object of this thesis was to determinate radiochemical purity of this antibody conjugated with bifunctional chelator DOTA and marked by luthecium, possibly purification of this antibody and consequently determination of its stability in different environments. In acetate buffer pH = 7, in an environment of the surplus of competitive ligand EDTA and in plasma of rats. Adequate analytic methods were used for these tasks: high-efficiency liquid chromatography, gel permeation chromatography and chromatography on thin layer. The results of the thesis demonstrated that this way of modification for further radiolabelling is not appropriate for large protein structures such as Nimotuzumab
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Physical interactions of the splicing factor Prp45
Kratochvílová, Eliška ; Folk, Petr (advisor) ; Doubravská, Lenka (referee)
It is well known that chromatin posttranslational state, transcription and splicing influence each other. Nevertheless, the details of this coupling are not fully understood. In S. cerevisiae, it is possible to induce conditions, in which splicing is uncoupled from transcription. Such situation occurred in cells expressing a mutated splicing factor Prp45, whose human homolog has been proved to participate in transcription regulation and also in splicing reactions. Based on previously indicated interactions in high throughput two-hybrid screens, we have been looking for physical links between Prp45 and proteins involved in chromatin posttranslational modifications. Finding of such a link would provide insight into the relationships of gene expression processes. Using coimmunoprecipitation and affinity purification, we were unable to detect physical interactions between Prp45 and our candidate chromatin regulators. Alternative approaches are discussed. Using the precipitation techniques, we mapped the interaction of Prp46 with truncated variants of Prp45. This observation contributes to our knowledge of protein-protein interactions within the spliceosome.
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Role fibroblastového aktivačního proteinu v růstu a invazivitě gliomových buněk
Fejfarová, Edita ; Bušek, Petr (advisor) ; Szabo, Pavol (referee)
High grade astrocytomas are very progressive brain tumors. Glioblastoma multiforme is the most frequent and the most malignant type with very infiltrative phenotype of the tumor cells. Fibroblast activation protein FAP is a predominantly membrane bound prolyl peptidase bearing exo- and endopeptidase hydrolytic activities. FAP is known to play a role in wound healing, cell migration and invasion and its expression is linked to the pathogenesis of several malignancies. mRNA expression of FAP is upregulated in 48% of glioblastomas according to The Cancer Genome Atlas microarray data. The involvement of FAP in the pathogenesis of astrocytic tumors is largely unknown. The aims of this work are to analyse the expression of FAP in primary cell cultures derived from high grade gliomas and to analyse the influence of FAP on the growth, migration and invasion of glioma cells. Our ELISA and western blot results showed heterogenous expression of FAP in the studied glioma primary cell cultures and cell lines. Both enzymatic activities characteristic of FAP were detected in the primary glioma cell culture P11 with high expression of FAP. In these cells, FAP was present not only in the typical plasma membrane localization, but also in the cytoplasm as demonstrated by immunofluorescence staining. The P11 cells...
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Molecular genetic and biochemical studies of selected inherited metabolic disorders, development and applications of new methods
Mušálková, Dita ; Hřebíček, Martin (advisor) ; Adam, Tomáš (referee) ; Macek, Milan (referee)
Inherited metabolic disorders (IMD) form a diverse group of several hundred different diseases with a relatively high cumulative incidence (stated up to 1:600). They are associated with accumulation of the substrates and lack of the products in specific metabolic pathways, which is caused by deficiency of the enzyme or its activator, or dysfunction of the transport protein. However, the underlying cause is at the DNA level. The grounds for different phenotype manifestation in patients with the same genotype are often not known. During my work at the Institute of Inherited Metabolic Disorders, I designed several new methods for the research of IMD and applied them in the patients and their families. I created procedures for the isolation of lysosomal membranes that are used for the research of lysosomal storage disorders and general properties of lysosomes. Next, I introduced several novel assays for determination of the X-inactivation ratio, which led to a significant increase of informative women. Nowadays, we use these methods in heterozygous women with X-linked diseases in order to study the influence of X-inactivation on the manifestation of the diseases. The cases of a girl with mucopolysaccharidosis type II, a girl with OTC deficiency and a family with the mutation in HPRT1 gene are described...
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