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Parallel single-cell analysis of active caspase-3/7 in apoptotic and non-apoptotic cells
Ledvina, Vojtěch ; Klepárník, Karel
Caspases are proteases that play key role in the process of apoptosis, the programmed\ncell death. Among them, caspase-3 and -7 are main executioner caspases that cleave\nmany vital proteins during apoptosis and after their widespread activation, the process\ncannot be reversed. To analyze caspase-3/7 activation within single cells, a miniaturized\ndevice for parallel analysis of eight samples was developed. The assay is based on the\nmodified luciferin-firefly luciferase bioluminescence (BL) system. Individual\nsuspended cells were collected and transferred into detection microvials using a\nmicromanipulator. The bioluminescence was detected using a photon counting head\nwith cooled photcathode. The LOD suitable for detection of active caspase-3/7 in both\napoptotic and non-apoptotic cells was reached.
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Philosophy of sport
Pilátová, Lucie ; Hogenová, Anna (advisor) ; Blažková, Miloslava (referee)
The thesis focuses on the philosophy of sport and historical analysis concerning the idea of the Olympic Games. Furthermore, the thesis aims to make the reader acknowledged with the historical beginnings of sport and evaluates various attitudes and changes concerning the initial sport philosophy and the perception of "Olympism" nowadays. More specifically we are trying to find the answer for the question how is sport in Antique different from its modern perception. In addition, the matter whether the change enables to imply the differences between the role of sport in the period of Antiquity and contemporary society, is being further discussed. The thesis is based on historical events and philosophical ideas of the origin of Antique Olympic tradition together with facts which led to the downfall and attenuation of sport in the medieval era. Moreover, it describes the birth of modern sport in its cradle in England and its revival in 19th and 20th century.
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The Finnish Landscape Photography and Its Role in Helsinki School
Bureš, Mojmír ; SILVERIO, Robert (advisor) ; Ledvina, Josef (referee)
In my bachelor thesis I am focused on the images of the Finnish landscape photography and relationship to nature due to the harsh geographical conditions. I will describe the political and cultural influences that affected the aesthetic thinking about perception of scanned landscapes. Furthermore, I would like to deal with the influence by art that have shaped and inspired the Finnish authors and compare it with the perception of the country abroad. For example Dieseldorf photography school (Bernd and Hilla Becher, Andreas Gursky), Japanese photographers (Hiroshi Sugimoto, Rinko Kawauchi) Czech photographers (Josef Sudek, Josef Koudelka) and the others .
List of the authors:
Into Konrad Inha, Caj Breme, Ismo HÖLTTÖ, Arno Rafael Minkkinen, Ilkka Halso, Maija Savolainen, Eeva Karhu, Kalle Kataila, Jyrki Parantainen, Elina Brotherus, Maanantai Collective, Mikko Rikala, Sandra Kantanen, Tiina Itkonen, Jorma Puranen, Susanna Majuri, Petri Juntunen, Eija-Liisa Ahtila, Nanna Hänninen, Pentti Sammallahti, Karl Emil Stahlberg, Riitta Päiväläinen, Marja Pirilä, Ritva Kovalainen, Sanni Seppo, Juha Suonpää
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Cultural support in Prague's districts
Mokrá, Jolana ; KAŠPAR, David (advisor) ; PROKOP, Petr (referee)
Diploma thesis ”Cultural support in Prague's districts” focuses on a current situation in municipal strategical management and support of culture in all Prague districts. The introduction emphasises the importance of each level of government in the management and support of culture through comparing specific cultural policies. The research is divided into two parts. First one depicts how culture is being understood on the municipal level and presents an overview of existing instruments for support and planning. The second part is focused on a few municipal districts that handle their culture policy with a particular strategy and consider it to be an irreplaceable part of a local development. The second part also examines what tactics and tools these municipal districts use for a realization of their strategic goals. In conclusion, this thesis proves why the role of the local official government is so important in the developement and implementation of culture.
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What makes realism real?
Skala, Adam ; NEBESKÝ, Jan (advisor) ; SCHEJBAL, Milan (referee)
The work consists of two parts. The first one focuses on the phenomenon of realism and realist tendencies during the process of creating a stage production. The work explores the evolution of the propensity for realist representation in the course of history. It focuses especially on the modern realism of the German director Thomas Ostermeier. It also tries to examine realism in its social context and define its role in society‘s development. The second part focuses on author‘s personal experience during creation of stage productions mainly, but not entirely, in work done for the university.
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Evaluation of the effectiveness of teaching vocational subjects depending on physical activity before schooling.
Janošíková, Martina ; Hanušová, Marie (advisor)
The period of transition to secondary school brings substantial changes in the requirements for study obligations, autonomy, communication, expression, or separated from their families in the form of staying at the dormitory. Students enrolled in sport centers, in addition to these changes, still struggling with the responsibilities associated with sport. Because they are students first through fourth grades of secondary schools and secondary vocational schools, an age when image self-I am, what I believe in, arises in interactions with the outside world and their own experience, plays a very important role to influence the environment, educators, teachers and trainers, with which students spend most of their time in practice. This study looks at students comprehensively in terms of personal development as a multifactorial biopsychosocial unit, in terms of cognitive and motivational processes, states and properties from the perspective of social psychology dealing with the interactions, relationships and communication, psychology of individuals within the social group and its importance for the development of the individual . It monitors conditions and educational outcomes in terms of educational psychology, dealing with forming properties, opinions, attitudes and value orientation from the perspective of educational psychology.
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The occurrence of domestic and wild forms of the camels in the old world and their economic use
Burgerová, Michaela ; Gardiánová, Ivana (advisor) ; Jan, Jan (referee)
Genus camel (Camelus) includes two species, the Dromedary camel (Camelus dromedarius) and Bactrian camel (Camelus bactrianus). Camels have a wide range of utilization. People in Arabia countries but also in India and Egypt are using camels as a milk and meat animal, or even as a transport and companion animals and riding animals too. The aim of this thesis was to find sources about camel keeping and using and his role of farm animal in different cultures and countries. And about conditions and requirements in captivity. The first part of thesis contains information about phylogeny and origin of the camel family, taxonomy of camels, short information about the history of domestication and using of camels. There are also described the two species in terms of species biology etc. plus a brief mention of camel hybrids and camel breeds. The second part focuses on the use of camels. The preffered and most common use is in the production of milk, other dairy products from camel milk, fermentation of milk. Another use is for meat performance, meat composition, describes products. His utilization i also useful for other parts such as hair wool and mane, they come mainly from bactrian camel (Camelus bactrianus). The last is working utilization of camels, carrying baggage. Then hobby use. The thesis describes the conditions of keeping camels in captivity, keeping camels in Somalia, requirements for the supply of water and food, housing conditions in the Czech Republic, it is particularly widespread keeping camels in zoos. In Czech Republic they are small private camel breeders. For comparison they are conditions for keeping camels in captivity in Australia.
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Development of electronisation of local administration of Zábřežsko micro region in relation with e-government
Filipová, Lucie ; Ulman, Miloš (advisor) ; Martin, Martin (referee)
Thesis "Development of an electronisation of local administration of Zábřežsko micro region in relation with eGovernment"examines the issues of the development of electronisation of autonomy in relation to legislation and eGovernment. The topic of the public administration has been increasingly discussed in recent years not only in the context of increasing demands for processing speed and information, but also to facilitate transparency and sub-processes in public administration. Theoretical part describes the role of eGovernment in the public administration, the process of electronisation of public administration and individual services of eGovernment (Czech POINT, data boxes, basic registers, communication infrastructure of public administration), which are being in place with municipalities. The practical part evaluates the situation of software and hardware equipment, legislatively bound on a contract software and service of eGovernment on a sample of villages Zábřežsko. On the basis of the results of the questionnaire researches are determinated recommendations for sample of 20 micro region Zábřežsko.
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