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Determination of athenticity of plant foods by molecular techniques
Plášková, Anna ; Mikulíková, Renata (referee) ; Němcová, Andrea (advisor)
The aim of presented diploma thesis was to determination of authenticity of fruit baby foods for early infant feeding using molecular methods. In the experimental part, isolation kit was used for isolation of plant DNA from fruits (strawberry, apricot, raspberry, apple) and from six commercial fruit products for children. Isolated DNA was characterized and verified using PCR methods with primers specific for plant rDNA (ITS2). Specific primer pairs were designed to amplify DNA for the detection of one fruit species. Primer specificity was assessed with four fruit species. A mixture of fruit puree from the two fruits was used to determine the sensitivity of the multiplex PCR assay. Six commercial fruit products were evaluated to verify the applicability of the multiplex PCR assay. The methodology of molecular detection of fruit DNA by qPCR and multiplex qPCR (duplex) includes approaches, which enable to detect two fruits (strawberry-raspberry, apricot-apple) in one reaction and thus reduces time and money requirements.
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Smart Home System
Hájek, Jaroslav
This work deals with connecting remoted devices and controling logics between them using Blockly. In the work is used lots of technologies for examples: MQTT, Websockets GSM, Lightweight Mesh and others. The Work is based on microservice which named is Flask. This service is an application interface for http services of Python programming language.
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System for controlling optical fiber amplifier modules
Matoušek, Jan ; Horváth, Tomáš (referee) ; Münster, Petr (advisor)
This diploma thesis deals with the design of the telecommunication device for optical amplifiers and the implementation of the universal software for thecommunication with an optical amplifier. An universal control interface has been created to control modules remotely via the SSH remote access or via the LCD display and buttons on the device. The Linux security is listed in this thesis. The device was designed in the 1U case, where the front and rear panels and the arrangement of elements inside were designed. Subsequently, the functionality of the device and the communication with the LCD display and the optical module were tested.
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Business Plan for Raspberry Paradise
Dudová, Nikola ; Zich, Robert (referee) ; Šimberová, Iveta (advisor)
This diploma thesis is worked out as a proposal for a business plan for establishing a small company in the area of agriculture primary production. The worked out business plan is based on analysis of the environment for the new company and from primary research. Theoretical part of the thesis deals with basic concepts which are related to business activities in the current condition. The proposition part consists of a proposal itself for the new business plan and the perspectives of implementing the plan and also risk that come with it.
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Flight Characteristics Control System for Autonomous Drone
Červenka, Ondřej ; Strnadel, Josef (referee) ; Šimek, Václav (advisor)
The goal of the bachelor thesis is to build a drone and implementation of safety measures in case of RC signal loss and also stabilizing the drone in the air using GPS and other available sensors. The thesis describes on history and development of the drones, their construction, components and parts of which it consists. Practical aspect focuses on building the drone, implementation of code for autonomous behaviour and testing of the final product.
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Use of Raspberry Pi in the home
Müller, Jan ; Luc, Ladislav (advisor) ; Veber, Jaromír (referee)
This work is devoted to description of Raspberry Pi computers, their commissioning and verification of their functionality by the author. The first part deals with the basic introduction of Raspberry Pi single-board computers, the Raspberry Pi Foundation and a short history of computer development. Three competing products of the present time are also described. In the practical part, the author describes how to get the Raspberry Pi computer to work for common computer users using the Raspbian operating system and memory card and everything is validated by the author. In the last phase of the work, the use of Raspberry Pi is described for household monitoring and for monitoring of various variables. The author of this work verifies that by connecting a Raspberry Pi camera module or various sensors such as temperature sensor or motion detection sensor. There will be briefly described other possibilities of using Raspberry Pi computers at the end of the thesis.
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Image processing on Raspberry Pi platform for mobile robotics
Kapitančik, Maroš ; Richter, Miloslav (referee) ; Horák, Karel (advisor)
This thesis deals with developing of image processing algorithm for robots controlled by informations taken from visual system. Core of the used system constitutes low-budget platform Raspberry Pi. Before the development of algorithm there is a series of test for image processing which discovers possibilities of used platform. Problem solution is divided to several parts. Limited performance frequently leads to individual problem solving. Afterall is shown sensitivity and performance analysis of developed solution.
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Smart garden
Dvořák, Jakub ; Kaczmarczyk, Václav (referee) ; Štohl, Radek (advisor)
The main objective of this work is created using Raspberry Pi intelligent garden. The first part is devoted to the concept of intelligent building. The second part describes the used hardware components. Another section is devoted SW Raspberry microcomputer and microcontroller Arduino. The last chapter describes the implemented model of a intelligent garden.
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Changes in selected local fruits during freezing.
Matějková, Markéta ; Melounová, Jitka (referee) ; Márová, Ivana (advisor)
This diploma thesis was focused on study of low-molecular antioxidants present in various kinds of fruits, namely berries. In theoretical part detailed characterization of antioxidants studied in presented work and their biological effects are introduced. Further, principals of processing and storage of specified species of fruits are discussed. Changes observed during storage of specific fruits are described too. In practical part changes in content of selected antioxidants in berries were followed. As studied material strawberries, raspberries, blueberries, red and black currants were used. All kind of these fruits was stored in fridge for six months using several different kind of processing. Berries were stored as whole fruits, whole osmotically processed fruits by sucrose, raw pulp and sweetened pulp. At first, starting values of selected antioxidant parameters were measured in fresh fruits (ascorbate, total antioxidant activity, total phenolics, catechins and individual flavonoids). After freezing the same parameters were measured regularly in two month periods. As the most suitable method of fruit processing, with regard to antioxidant content, storage of the whole fruits or pulp can be recommended. Content of ascorbate decreased in all forms and species of the processed fruits during all the time of storage. Oppositely, the content of other studied antioxidants exhibited fluctuations or increasing trend. Sensory analysis was proved for the whole fruits and also for whole sweetened fruits.
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