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Bioflavouring of low alcoholic beer by dry hopping
Dušánek, Václav ; Mikulíková, Renata (referee) ; Pořízka, Jaromír (advisor)
This bachelor thesis deals with the influence of cold hopping of low-alcohol beers on analytical properties and sensory profile of beer. The effect of cold hopping was observed using the varieties Kazbek, Jazz, Mimosa and Uran. In the experimental part, the production process of low-alcohol beer using immobilized yeast is described. After the main fermentation, the low-alcohol beer was kegged into five cuvettes and after 20 days cold-hopped at a dose of 3 g/l with individual varieties. The effect of cold hopping with individual varieties on the organoleptic properties of the beer was observed. Bitterness was determined, with cold-hopped beers showing an increase between 17-24 % in bitterness. Furthermore, the ethanol content was determined by high-performance liquid chromatography at 2,14 % vol. A significant increase in all volatiles, in particular -myrcene and linalool, was found in the cold-hopped beers by gas chromatography with mass spectrometry. The sensory analysis showed that the cold hopping had a positive effect on the overall impression and masked the taste defects of the reference low-alcohol beer. The best hop evaluated for the use of the cold hopping method was the Kazbek variety. The Jazz and Mimosa varieties were also perceived relatively positively by the evaluators. On the other hand, the Uran variety achieved a worse overall impression than the reference beer.
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Production of pates with the addition of mushrooms
Tóthová, Martina ; Holub, Jiří (referee) ; Mikulíková, Renata (advisor)
This bachelor thesis deals with the sensory evaluation of pâté with mushrooms. First the 3 samples of pâtés were made to select the right concentration of mushrooms for sensory evaluation. Then the 7 samples of pâtés which contained different texture and types of mushrooms were produced. In the theoretical part pâtés are characterised, their manufacturing and types of pâtés are described. The part of literature search is description of mushrooms in general, their composition and description of mushrooms which were used for production of pâtés. Another topic is description of conditionals and methods of sensory analysis. The experimental part is focused on the production of pâtés for two sensory analyses, evaluating the heat treatment and sensory analysis. The content of vitamin D in the samples of pâtés was analysed by liquid chromatography. Processing of results of sensory analysis of pâtés was determined that the best samples were pâtés with milled champignons and champignons which were add in blender.
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Technology for the production of wine sausage with the addition of cheese
Kejmarová, Anna ; Nábělek, Jakub (referee) ; Mikulíková, Renata (advisor)
The aim of the bachelor thesis was to investigate the effect of soft cheese on the organoleptic properties of wine sausage. Samples of wine sausages were taken before and after baking and the percentage of lipids in the sample and the content of individual fatty acids were determined using the analytical method of gas chromatography. The sensory analysis was divided into two parts. In the first part, the evaluators assessed the appearance, aroma, consistency, texture, taste, overall evaluation and balance of the cheeses. The second part focused on the overall evaluation and selection of the most flavourful samples. The data obtained from the sensory analysis were processed and evaluated using Excel. The most unpalatable wine sausage, from the results of the sensory analysis, was the wine sausage with ermine.
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Possibilities of using spices in the production of ham salami
Vershinina, Alina ; Szotkowski, Martin (referee) ; Mikulíková, Renata (advisor)
This bachelor's thesis focuses on the possibilities of using different spices, specifically cumin, nutmeg, allspice and marjoram, in the production of ham cuts. The aim of the work is to investigate the effect of these spices on the taste, aroma and other sensory properties of the ham cut and to assess their contribution to improving the overall quality of the product. The methodology includes the collection of samples of ham cut with various additions of spices, the performance of sensory analysis and chemical analysis for the identification and quantification of phenolic substances present in individual spices. Subsequently, the results will be evaluated and their significance for the production of ham cuts will be discussed. The results of this work could contribute to a better understanding of the use of spices in the production of meat products and provide data for the optimization of recipes and processes in the food industry.
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Study of the release of active substances from PHA-based particles
Čech, Jan ; Mikulíková, Renata (referee) ; Kovalčík, Adriána (advisor)
This bachelor thesis deals with the study of the release kinetics of caffeic acid and acetylsalicylic acid from polylactic acid, poly(3-hydroxybutyrate-co-4-hydroxybutyrate) and poly(3-hydroxybutyrate)-based microparticles. In the theoretical part, the mechanical and thermal properties of polyhydroxyalkanoates, their biological synthesis by microorganisms, their industrial extraction from biomass and applications are described. Furthermore, the properties of PHA microparticles, their use as carriers, the most common microparticle preparations and the release method of the loaded drug are described. In the experimental part, PLA, P(3HB) and P(3HB-co-4HB) based microparticles prepared by the solvent evaporation emulsion method were characterized by dynamic light scattering. Subsequently, caffeic acid or acetylsalicylic acid was incorporated into these microparticles, and the release kinetics of the active ingredient was observed spectrophotometrically for one week. Once measurement of the releasing kinetics ended, microparticles were again measured by DLS and compared with the characterized microparticles before releasing kinetics. Finally, the encapsulation efficiency and microparticle recovery were calculated.
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Use of antioxidants in the production of white sausages
Richterová, Nikola ; Pořízka, Jaromír (referee) ; Mikulíková, Renata (advisor)
This thesis focuses on the use of antioxidants in the production of white sausage. Two herbs were chosen as representatives of natural antioxidants (Salvia officinalis and Rosmarinus officinalis), whose effects were compared with the synthetic antioxidant butylhydroxytoluene. Furthermore, this thesis discusses the possibilities of using the FoodScan 2 device for the determination of basic nutritional characteristics in the produced sausages. The impact of antioxidant addition was evaluated, especially on the sensory quality and consumer acceptability of the manufactured sausage. Herbs were added to the sausages in dried, ground form and in the form of prepared ethanol extracts. The impact on the oxidative damage of the sausages during storage in cold (4 °C) for five days was also examined. The protein, fat, water, and ash content in the sausage samples was determined using the FoodScan 2 device, the results were compared with those acquired using simple analytical methods. On the day of production and then five days of storage in cold, the lipid content and fatty acid profile in the sausage samples were examined by gas chromatography, and the content of substances reactive with thiobarbituric acid in the sausage samples was examined by spectrophotometric analysis. The antioxidant activity of the samples was also measured. Furthermore, the manufactured sausage samples were subjected to microbial control. The prepared ethanol extracts and butylated hydroxytoluene were analysed for antimicrobial activity against Escherichia coli and Micrococcus luteus. For most evaluators, the herbs and their extracts that were added to the meat product appeared tasty and consumer friendly. Statistically significant differences were found between the samples using the Kruskal-Wallis test. In produced samples, herbs exhibit comparable efficiency to synthetic butylhydroxytoluene and could be a suitable alternative to synthetic food additives.
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Optimization of the biotechnological processing of lipidic food wastes by carotenogenic yeasts on a laboratory and pilot scale.
Plhalová, Žaneta ; Mikulíková, Renata (referee) ; Szotkowski, Martin (advisor)
This thesis is aimed at monitoring the production characteristics of carotenogenic yeasts when cultured using waste substrates as sources of organic carbon and energy. The waste substrates tested included waste fryer oil, chicken rendering waste fat, coffee oil and coffee hydrolysate derived from coffee grounds and waste glycerol. The producers investigated were yeasts Cystophilobasidium infirmominiatum (CCY 17-18-4), Cystophilobasidium macerans (CCY 10-1-2), and Rhodotorula kratochvilovae (CCY 20-2-26), Rhodotorula mucilaginosa (CCY 19-4-6), Rhodotorula toruloides (CCY 62-2-4), Sporidiobolus pararoseus (CCY 19-9-6), Sporidiobolus metaroseus (CCY 19-6-20) and Sporidiobolus salmonicolor (CCY 19-6-4). Among the metabolites studied were carotenoids, ergosterol, ubiquinone, tocopherol, lipids, fatty acid composition and beta-glucans. Lipase activity was also monitored. The thesis has two main parts: theoretical and experimental. The theoretical part describes the different genera, the metabolites studied and their functions, and the waste substrates used. In the experimental part, the methods used for the preparation and treatment of waste substrates, media preparation, yeast cultivation, biomass acquisition and processing, sample preparation for individual analyses, and the metabolite analysis itself were described. From the screening cultures, it was found that the most suitable strains for cultivation using waste media were Sporidiobolus pararoseus, Rhodotorula mucilaginosa, Rhodotorula toruloides and Cystophilobasidium macerans. The strain Sporidiobolus pararoseus achieved the highest biomass production and significant enrichment of biomass with the metabolites studied. Based on screening cultures, several large-scale cultures were performed in a laboratory bioreactor, where the best increase was achieved by the strain Sporidiobolus pararoseus (37.5) g/l.
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Use of the addition of aromatic herbs in the production of pates
Mejzlíková, Blanka ; Hoová, Julie (referee) ; Mikulíková, Renata (advisor)
This diploma thesis deals with the production, characterization and senzory evaluation of pâtés with aromatic herbs. Specifically levander (Lavandula angustifolia), coriander (Coriandrum sativum), savory (Satureja hortensis), sage (Salvia rosmarinus) and hyssop (Hyssopus officinalis). The theoretical part is focused on the description of meat and meat products, pâtés, their composition and production technology. Part of the theoretical part is also a description of the aromatic herbs and the possibilities of their use in meat products, it also deals with methods and conditions of senzory analysis. In the experimental part, the production of pâté with aromatic herbs was optimized, specifically the amount of liver and the amount of aromatic herbs were optimized. The experimental part also deals with the production of pâtés for senzory analysis, the senzory analysis and the characterization of pâtés. The produced pâtés were characterized in terms of selected nutritional characteristics (content of fats, fatty acids, carbohydrates and proteins), it also were characterized in terms of water content, antioxidant activity, total phenolic substances and volatile substances. Processing the results of the senzory analysis, it was found that in terms of acceptability, all pâtés with aromatic herbs were rated worse than the standard (pâté without herbs). The addition of herbs had a positive effect on the antioxidant activity of the produced pâtés and the associated nutrional value of the pâtés.
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Processing of sauerkraut juice to enhance its stability and consumer acceptance
Kohoutová, Lenka ; Mikulíková, Renata (referee) ; Hoová, Julie (advisor)
This diploma thesis focuses on the development possibilities of fruit and vegetable juices containing sauerkraut juice, the characterization of their nutritional and sensory qualities and their stability, as well as the potential for enriching selected juices with probiotic culture of Lactobacillus plantarum. The best combinations of various juice mixtures were selected based on sensory analysis and were stored for 30 days at 8 °C. Changes in selected parameters were monitored during the storage. Antioxidant activity, total flavonoids and polyphenols content and content of total water-soluble and reducing saccharides were determined by spectrophotometric methods. HPLC was used for lactic and L-ascorbic acid determination, flow cytometry was used for probiotic cell content determination, culture test was used to observe viability of cells, pH was monitored. Contents of antioxidant activity and of total polyphenols content did not drastically change during the storage. Major changes were observed in total and reducing sugar content and total flavonoids content. It was observed that probiotic viability was preserved for 14 days, decrease occurred afterwards. In order to achieve as high content of probiotics as possible, it would be advisable to store probiotically enriched juices for maximum of 14–19 days at temperature of 8 °C. The highest viability was observed in JHM sample (containing apple, pear, carrot and sauerkraut juice in the same ratio). Even through viability of probiotic cells was determined as low, these presented functional beverages remain nutritionally, visually and taste-wise interesting products, which can help popularize the consumption of non-traditional local foods.
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Development of a recipe for white sausage with a share of vegetable protein and fibre
Blahutová, Petra ; Svoboda, Zdeněk (referee) ; Mikulíková, Renata (advisor)
The thesis is focused on the development of a recipe for white sausage with a substitute of vegetable origin. Red lentils and root vegetables represented by carrots, parsley and celery were chosen as substitutes. Three sets of sausage samples with four different concentrations were prepared according to the optimized recipe. The samples prepared in this way were subjected to a sensory analysis in order to determine the optimal concentration of the substitute in the sausages.The content of selected nutritional characteristics, specifically proteins, fats and fatty acids, carbohydrates and water, and their trends were determined for the prepared samples. With the exception of a few, gradually increasing or decreasing trends in the contents of nutritional parameters were detected depending on the abnormal concentration of the substitute. Data from sensory and nutritional testing were subjected to statistical evaluation. Regarding the sensory parameters, it was shown that the samples with low and high content of substitutes are significantly different from each other across the sensory parameters. The statistical correlation of the sensory and nutritional evaluation showed that the sausage samples with higher fat content and low substitute content were evaluated as tasty, which means that there is a direct correlation between the fat content and the overall rating of the sausage. The sample with 36 % of the substitute remaining from a 1:1 mixture of red lentils and root vegetables was chosen as the best sample. It was the most sensory-pleasing to consumers and at the same time enriched with a plant component.
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