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Testing of food authenticity using DNA analysis
Kocianová, Vanda ; Rittich, Bohuslav (referee) ; Španová, Alena (advisor)
Authenticity verification of food products is an important step before their release to the market. This testing ensures that the product contains what the label states and also confirms the medical harmlessness. One of methods possible to use for authenticity verification is polymerase chain reaction (PCR). The experimental part was focused on the DNA isolation using magnetic particles on PCR – ready quality from food product – ketchup. DNA isolated from seeds and stalk of tomato were used as a controls. Amplificalibity of DNA isolated with magnetic particles using magnetic separator and needle was compared. The method requires further optimization.
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Study of the authenticity of chocolate with different content of cocoa solids
Vanduchová, Petra ; Dvořák, Miloš (referee) ; Diviš, Pavel (advisor)
This master's thesis deals with authenticity of chocolate with different content of cocoa solids. The theoretical part describes the production of chocolate products and the issue of food authenticity. Experimental part of this thesis is focused on the determination of total cocoa solids in the selected samples. Different samples of chocolate containing 50–90 % of cocoa were grated to fine particles, that were subjected to extraction without previous chemical treatment. The methylxanthines theobromine and caffeine were detected by HPLC to determine the content of fat–free cocoa solids. Fat–free cocoa solids content were found in the range 19,7–43,1 g/100 g of dry matter and the content of total fat was determined by the gravimetric method and was found in the range 30,9–55,4 g/100 g. The results of this thesis prove that, the producers meet the legislative requirements and the declared values are corectly declared on the wrappers of the product.
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Validation of HPLC method for determination of organic acids in beverages
Hanychová, Silvie ; Divišová, Radka (referee) ; Diviš, Pavel (advisor)
This diploma thesis focuses on validation of RP–HPLC method for determination of organic acids in fruit juices. The aim of this thesis was to determine suitability of the method for verification of the fruit juices authenticity based on analysis of organic acids. The theoretical part describes chemical composition of fruit and fruit juices and focuses on organic acids. It provides description of various methods of organic acids analysis with more detailed description of High Performance Liquid Chromatography and its validation. The experimental part focuses on optimalization and validation of the method. Samples of malic, citric, succinic and ascorbic acid were analysed. The optimalized method was validated and following performance parameters were determined: repeatability, linearity, limit of detection (LOD) limit of quantitation (LOQ). RP–HPLC method was applied to real samples of 100% fruit juices (apple, orange and grapefruit) and the authenticity parameters were verified.
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Authenticity and Digital Information
Cubr, Ladislav ; Ivánek, Jiří (advisor) ; Sklenák, Vilém (referee) ; Hutař, Jan (referee)
The dissertation focuses on the authenticity of digitized books in the context of their life cycle (production, preservation, access). First the OIAS high-level conceptual framework for lifecycle management of digital documents maintained by organizations is introduced. Then the current situation of digitized books lifecycle management is described. This part is followed by an introducing to relevant conceptualizations of the authenticity of digital documents and these conceptualizations are analyzed and reviewed. Then framework for analysis of authenticity is established based on previous findings. This framework is then used to identify authenticity requirements for digitized books and to develop a domain-specific conceptualization of the authenticity of digitized books. This conceptualization deploys detailed analysis of risks threatening authenticity during lifecycle management of digitized books. The selected topics of this conceptualization are then the source for the next step, which is to develop a recommended practice for maintaining authenticity of digitized books. This practice is further specified for one partial solution to the problem of maintaining the authenticity of digital documents throughout their life cycle, which is a persistent identification system.
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"Folklorní mejdlo": Contemporary Folklorism in Prague
Hrbáčková, Anežka ; Jurková, Zuzana (advisor) ; Seidlová, Veronika (referee)
Prague as space, inside of which music of various genres is being created and performed, linked to different values, being created on foundations of distinct concepts and serving as a medium of various strategies, is characterized by fluid and difficult to grasp soundscape (Shellemay 2006). This environment allows the creation and negotiation of traditions (Glassie 1995), moving into different directions. One of them, which have a recursive character and serves its actors as the construction of meaning and carrying out sociocultural change for its actors, is connected with the concept of folklore. The presented fieldwork examines this part of contemporary Prague soundscape in the field, framed by one of the music and dance events. The regular event, named by the organizers "Folklorní mejdlo", defines itself as a connection of the world of modernity, which is currently offered in the metropolis, and the world of traditions, authenticity and folklore. The fieldwork aims its focus on the way the concepts of authenticity and legitimacy are negotiated, its assigning to individual actors and objects and generally how the actors of the event undertake the construction of folklore and how the final product looks like. This work, with its approach to music as a social act (Turino 2008) endorses the...
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Falsification of paintings and its impact on the Czech art market
Bergerová, Anna ; Janištinová, Anna (advisor) ; Prokůpek, Marek (referee)
The aim of this thesis is to analyze the impacts of the falsification of paintings on the art market and on individuals. Another aim of the thesis is to identify the risk factors of buying art works on the Czech market. The partial aim is to bring to readers a comprehensive view of the issue of the falsification of paintings on the domestic art market. The theoretical part is based on the literature that has not been published so far and maps the basic mechanisms of falsification of paintings. The practical part is based on interviews with experts, criminologists and collectors who have a personal experience of buying a forger. Based on the results of the interviews, the issues of falsification of painting works are answered, the most frequently falsified Czech artists are identified and the risk factors that lead to the purchase of counterfeit. Three artists demonstrate an analysis of expertise and forensic technique in determining the authenticity of the art work.
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The boundaries of ecotourism and their crossing on the community in Chambok
Kořánová, Anna ; Kuřík, Bohuslav (advisor) ; Tenzin, Eva (referee)
My thesis focuses on the problems of ecotourism, or more precisely its borders from an anthropological perspective, using example of a typical Cambodian community named Chambok. Based on five months of research, together with ethnographic methods of research I found out that boundaries of ecotourism are complex, multi-layered, problematic and negotiated and also that there are so called "grey zones" encompassing them. It is the conceptualization of these zones and boundaries, which is my contribution to current anthropological debate about ecotourism, which has been largely left out until now.
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Thriving (glourishingg) of supra-national corporation manager
Rohlíková, Petra ; Rymeš, Milan (advisor) ; Bedrnová, Eva (referee) ; Štětovská, Iva (referee)
A managerial role place great challenges on the individual from the perspective of self- management, primarily considering the challenges of that role and individual needs and values. This dissertation discusses the potential for maintaining well-being when performing in a managerial role in a supra-national corporation. The focus is primarily on the idea of flourishing (thriving) defined by the American psychologist Martin Seligman. Flourish fundamentally includes subjective, hedonic elements (experiences of joy), as well as, eudaemonic quality built on the individual but for the wider community. Flourishing is closely linked to the self-concept of the individual; its content is subjective in character. Employment in a supra-national environment includes characteristics which may reduce the quality of this for an individual. This, mainly, involves constant adapting to change, working in a turbulent environment, collaborations encountering cultural differences (national and organisational), working in a matrix structure and/or frequent emphases on hedonism or consumerism. The research is predominantly qualitative in character; mapping pivotal topics which, in the future, will require a more empirical approach. This research focuses on a deeper understanding of functional areas and strategies which...
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