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Development of Methods for High-Throughput Enrichment of Glycoproteins and Glycopeptides Employing Multiple Lectin Affinity Chromatography/Tandem Mass Spectrometry
Maděra, Milan ; Pacáková, Věra (advisor) ; Feltl, Ladislav (referee) ; Foret, František (referee) ; Kučerová, Zdenka (referee)
5 CONCLUSIONS This thesis surrrnrarizes the development of a multimethodological analýical approach employing microcolumn lectin affi1t1,9fuom1tography coupled to highir".otu,to; separation and dete9tion techniques, in order to facilitate the enrichmení of glyco-proteins aná giycopeptioes originating from small quantities of real samples. This is u"toauy a sipificant-ítep towara satis$'ing the demands of contemporary glycoproteomics, which rely orirurt *a small scale analyses, allowing the identification of low abundant protein components' The overď conclusions and contribution to the contemporaÍy scierrce are drawn in tbree followine chaoters. 5.1 combining Lectin Microcolumns with High-Resolution separation Techniques for Enrichment of Glycoproteins and Glycopeptides This section describes coupling small-scďe lectin aÍfinity chromatograpby on-line to high- resolution separation and detection techniques, with the utiliý of a microcolumn loaded with a lectin immobilized onto macroporous silica. Experimental results, involving optimization of coupling procedure, fabrication of lectin microcolumns, verification of tteir Uin&ng efficiency and their possibility of interfacing on-line with nano LC-MS/]víS, me summarizžd into thé following conclusions; o optimized coupling procedure involved resuspending only 125...
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High-performance chromatographic methods for determination of proteins
Vařilová, Tereza ; Pacáková, Věra (advisor) ; Feltl, Ladislav (referee) ; Coufal, Pavel (referee) ; Kučerová, Zdenka (referee)
Conclusions This Thesis gives an overview of high-performance riquid chromatography in various separation modes for a characterization of selected proteins. various affinity stationary phases were prepared for analysis of pepsin, seminal plasma proteins and selected glycoproteins' choice of the affinity system suitable for a particular purpose is always a comprex probrem in which number of aspects must be considered. properties of the proteins to be separated and ligands used had to be taken into an account. The separation system should not influence the biorogicai activity ofproteins separated. From this point of view it is clear that for any each individuar system is necessary to find specific conditions for I) immobilizationof ligand, and II) separation conditions. Stationary phases with immob'ized 3,5-diiodo-L-tyrosine exhibited sufÍicient selectivity, proved to be useful for analysis ofporcine pepsin A and are appricable to practical sampres of human pepsin' Reversed-phase crs corumn is suitable for anarysis ofpepsin fragments obtained after a-chymotrypsin digestion. A study oť interaction of various forms of pepsin and pepsinogen are essentiar because of their high importance as crinicar diagnostic markers and as stomach enzyme. "lA IŤ Aťfinity chromatography with heparin immobilized to Toyopearl...
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How to reduce overheating in Czech towns and village
Hermanová, Veronika ; Kučerová, Zdeňka (referee) ; Pavlovský, Tomáš (advisor)
The aim of this bachelor thesis is to point out raising temperature and climate changes in towns and villages in the Czech Republic. It focuses on the impact of climate changes on health of population as well as impact on public places. It analyses and proposes all possible conceptual solutions to support sustainable development and reduction of the climate impact. The target is to indicate measures of help to nature that will navigate to positive response on the climate changes on streets and public places to achieve comfortable living in the build-up area. Part of the bachelor thesis is personal proposal for the modification of the given public space in the town of Valasske Mezirici, to which individual measures are specifically projected.
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Zdravotní gramotnost populace v prevenci alimentárních nákaz
KUČEROVÁ, Zuzana
The bachelor thesis called "Health literacy of the population in the prevention of foodborne diseases" deals with diseases caused by food contaminated with microorganisms and their prevention. The theoretical part includes characteristics, distribution of foodborne diseases, definitions and detailed description of selected diseases. The next section describes the causes of foodborne infections and their classification into three groups: poor personal hygiene, multiplication of micro-organisms and contamination. Food safety and the importance of hygiene measures, guidelines and legal standards that need to be known in order to prevent these diseases are also described. The last part of the bachelor thesis defines the duties of food service workers. The subject of research of the practical part is the health literacy of workers in closed and open mass catering establishments who are involved in the preparation of food and therefore work in activities of epidemiological importance according to the Public Health Protection Act. The survey maps their knowledge of good hygiene practices in food preparation, distribution and storage. The second part of the practical part consists of the analysis of menus from selected establishments and their analysis in the NutriServis application. The results showed that health literacy in preventing foodborne infections is good. They are aware of epidemiologically relevant activities, terms and laws related to them. Again, these respondents have a good understanding of personal and operational hygiene, compliance with food preparation rules, and institutions dealing with, for example, emerging epidemics of alimentary diseases. The second part of the bachelor thesis focused on the evaluation of the weekly menu and the analysis of potential risks of foodborne illnesses. It was concluded that undercooked meat, meat products, eggs and unwashed fruit and vegetables could be the greatest risks.
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Indicatours of sustainable development social pillar at the local level
Kučerová, Zita ; Kyselka, Mojmír (referee) ; Doc.PhDr.Jana Šafránková,CSc. (referee) ; Staňková, Eva (referee) ; Koutný, Jan (advisor)
The aim of the thesis is to find the answer, whether it is plausible to monitor social cohesion of the municipality and how to measure this cohesion, which is one of the essential pillars of sustainable development. In order to find out, analysis of approaches and measurements of the sustainable development social pillar in international and Czech environment was carried out. Social pillar was identified and described also at the local level. Within the framework of the sustainable development social pillar at the municipal level three main thematic blocks are proposed: • Social environment quality of the municipality • Social-demographic population structure of the municipality • Contentment and participation in the public affairs These themes should be monitored by the set of indicators proposed in this thesis. It aimed to cover all dimensions of social cohesion which are significant and well-founded in the context of the territorial planning process. These indicators can be followed in the direct relationship with the population size and regional importance of the observed territorial unit at the local level and they are not separated from the concept of indicators monitoring at other – territorially higher – levels. Outcomes, analysis and interpretation of attained values could become the basis for the “territorial sustainable development analysis” (part of territorial planning instruments according to contemporary legal regulations), representing social pillar. All phenomenon and processes must be evaluated in the context with other sustainable development pillars and afterwards they should become another suitable instrument for measurement of the fulfilling the territorial planning aims.
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Study of substance transport via membrane transporters
Kučerová, Zuzana ; Smutná, Lucie (advisor) ; Maixnerová, Jana (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Student: Zuzana Halamová Supervisor: PharmDr. Lucie Smutná, PhD. Title of diploma thesis: Study of substance transport via membrane transporters Wide range of specialized transport proteins expressed mainly in the kidney proximal tubule ensures the excretion of endogenous and exogenous substances. OCT2 (organic cation transporter 2) is localized on the basolateral membrane of the kidney proximal tubule, the transporter transports broad spectrum of structurally different substances. Drug-drug interactions may occur at the level of this transporter due to its broad substrate specificity. Within this diploma thesis the accumulation of the radiolabeled antiviral agent [3 H]lamivudine and the model hOCT2 substrate [3 H]MPP+ was evaluated using accumulation and transport studies. The canine kidney cells MDCKII or human cell line HeLa were used as model cell lines. Mentioned cell lines expressed the human OCT2 transporter stably or transiently. The antibacterial chemotherapy agent trimethoprim was used as competitive inhibitor of OCT2 transporter. The results indicate the lamivudine accumulation in the cells with OCT2 is low compared to accumulation of MPP+ . Accumulation studies showed that lamivudine had a...
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Selected Works of Pavel Čech in Leisure Education for Reading
KUČEROVÁ, Zuzana
The bachelor thesis aims to introduce the work of Pavel Čech (an illustrator, a comics creator, and a book writer) and the use of selected work in leisure education indicated for reading. The dissertation is divided into two parts. The theoretical part introduces a bio, author's work, awards received, preferred literature genre, and selected methods chosen for particular work with text. The practical part focuses on the project O čertovi that develops the reading in kinder garden.
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The role of the program The leader in me in developing competencies
Kučerová, Zuzana ; Smetáčková, Irena (advisor) ; Frombergerová, Anna (referee)
The subject of this work is to describe and present the program The Leader in me as one of the possible starting points of the current educational effect on students of all levels of the educational system in terms of their equipment with competencies. The theoretical part defines the basic concepts related to the framework educational program, key competencies in education and the school educational program. Furthermore, the principle of 7 habits of effective behavior is described here and it is stated how they can be applied in the school environment with the help The practical part of the work focuses on the methods leading to the acquisition of key competencies within the classes of Personal - Social Education and on the possibilities and impact of introducing of the program The Leader in me into practice at the Primary School Bratří Čapků in Ústí nad Orlicí. The information was collected through interviews and follow-up questionnaires. This research showed that the location of Personal and Social Education as a separate subject, its content and outputs, are in accordance with the recommendations of The Framework educational program for basic education and the thinking and intentions of class teachers. Furthermore, it turned out that despite the fact that, according to teachers, the principles...
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Legal aspects of the circular economy
Kučerová, Zuzana ; Sobotka, Michal (advisor) ; Falteisková, Sabina (referee)
Legal aspects of the circular economy Abstract The diploma thesis deals with the issue of legal aspects of the circular economy. This concept is based on resource efficiency with the intention to minimize waste generation. This approach is considered to be the future of economies not only in the European Union, but also in the rest of the world. The thesis aims to provide a comprehensive overview of the circular economy and the legal background of this concept in the EU and the Czech Republic. Given that the legislation of the circular economy has so far been reflected at the EU and national level predominantly in the field of waste management, it forms a significant part of this thesis. The projection of the principles of the circular economy into the legal order as well as the future development of this concept is evaluated. Recognized advantages and disadvantages in the current circular economy legislation are formulated at the end of the thesis. Subsequently, the thesis sets recommendations for the future direction of the circular economy and for its efficiency enhancement.
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Transformation of the values of the individual by Friedrich Wilhelm Nietsche and Søren Aabye Kierkegaard.
Kučerová, Zuzana ; Blažková, Miloslava (advisor) ; Hauser, Michael (referee)
Goal of this work is to show that the values in European and a traditional context could be transformed. I chose two philosophers F.Nietzsche and S.Kierkegaard where I can document this change. Just the two of them proved to be very important for the 20th century .In the main part of my work, I am describing and comparing the evolution of the axiological views of both philosophers. My study on existential philosophers includes topics such as freedom, sense of life, base of christianity, perception of human being. Nietzsche and Kierkegaard are philosophers who have a lot in common, but they would not get on well with each other. The things that they share are the same time what they deal with in a difááé=ferent way. They treat topics such as existence, futility, strength of faith. However Kierkegaard studies in depth existentialism, immersion in irrationality, desolation. In Nietzsche we can find the will of power, the nihilist opinions and the criticism of christianity.
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