|
| |
|
|
Development of effective procedures for transplantation of insulin-producing tissue
Hladíková, Zuzana ; Saudek, František (advisor) ; Kvapil, Milan (referee) ; Průhová, Štěpánka (referee)
Our research has been focused on a new method of transplantation of islets of Langerhans into the greater omentum. Transplantation of pancreatic islets is an established treatment option for selected patients with unstable diabetes. The liver is almost exclusively used as a site for islet transplantation in the clinical setting. Unfortunately, immediately after graft implantation into the portal circulation, a substantial portion of the islets is lost and graft function in the liver may further deteriorate over time. The fate and location of islets implanted in the liver can only be followed with difficulty by radiological methods or biopsies. All these limitations have led to the search for alternative sites for transplantation. The greater omentum is easily surgically accessible, meets the demands of a high blood supply and can accommodate large volumes of transplanted tissue. However, the metabolic function of the islet graft itself in the omentum without fixation never achieved satisfactory long-term results in the experiment. Tissue engineering techniques have been used to improve graft attachment and maintain long-term function in the omentum. In our project, we transplanted islets into the rat omentum using a biocompatible gel consisting of recipient plasma and human thrombin....
|
|
|
Comparison of penile blood flow changes in performance road cyclists obtained during a ride on a customized 3D printed and conventional saddle
Šňupárek, Václav ; Vomáčková, Helena (advisor) ; Martínek, Milan (referee)
Comparison of penile blood flow changes in performance road cyclists obtained during a ride on a customized 3D printed and conventional saddle Author: Bc. Václav Šňupárek Supervisor: PhDr. Helena Vomáčková, Ph.D. Aim: To update findings on penile blood flow in performance cyclists by measuring transcutaneous partial pressure of O2 (tcpO2) and CO2 (tcpCO2) on current style of racing bicycle saddles Subjects and methods: tcpO2 and tcpCO2 were measured non-invasively on the glans penis in healthy highly trained male cyclists (�ළ = 30) with an average annual mileage of 14240 km on three different road cycling saddles - Posedla Joyseat, Specialized Power and Fizik Tundra M5. It has previously been shown that tcpO2 is highly correlated with arterial pO2. TcpO2 and tcpCO2 were measured continuously in each participant for ten minutes on each saddle with a random order of saddles while riding stationary bike trainer in a race fit position. Simultaneously, various data like saddle surface pressure via Velometrik Smart Cover BT, rider position via Retül Fit, power output, heart rate and cadence via Wahoo KICKR Bike were recorded, and glans penis biothesiometry data were collected with Bio Medical Instruments. Data analysis was done on statistical level of significance �ළ < 0,05. Statistical methods used to...
|
|
|
Studies to discover new representatives of heme sensor proteins
Pašek, Dominik ; Martínková, Markéta (advisor) ; Dračínská, Helena (referee)
Hemoproteins are diverse and very important group of proteins involved in a wide range of biochemical processes. Their typical roles in living organisms include oxygen storage and distribution, enzymatic catalysis of redox reactions and electron transport in the respiratory chain. In relatively recent times, a fourth group of hemoproteins, the so-called heme sensor proteins, has been discovered. This group includes, among others, proteins that have the ability to reversibly bind heme, which in such cases acts as a signaling molecule. This reversible interaction is essential for cells in certain signalling pathways. As it was found, one of the heme sensor proteins is the p53 protein, which is an important transcription factor The first phase of this diploma thesis was focused to the preparation of p53 family proteins including some of their deletion mutants. Subsequently, the task for selected proteins was to study the influence of heme on their oligomeric state. The essential part of the thesis consists of spectrophotometric studies, which aimed to reveal potential interactions of the studied proteins with heme and to characterize the interactions. Keywords: heme, heme sensor proteins, p53 protein family, transcription factors, signal transduction
|
|
|
Transition of a young person with an intellectual disability to adulthood from the perspective of caring parents
Kubišta, Ivan ; Šiška, Jan (advisor) ; Šumníková, Pavlína (referee)
This bachelor thesis deals with the topic of the transition of people with an intellectual disability into adulthood from the perspective of their caring parents. It addresses the question: How do caring parents proceed in the process of transition of their son/daughter with an intellectual disability into adulthood and how does this process work from their perspective? The aim was to explore the process of transition of persons with an intellectual disability into adulthood from the perspective of caring parents and to contribute to a better understanding of this issue based on the results of the research conducted. The literary research was used to develop an informative research preparation that summarizes the theoretical background and the current state of knowledge of the chosen topic. Three parents of people with an intellectual disability from three different families, who have current experience with their transition into adulthood, participated in the qualitative research. The data were obtained through semi-structured interviews conducted in the informants' home environment. The research included answering six research sub-questions that specify the area of transition of people with an intellectual disability into adulthood from the perspective of their caring parents. The transition of a...
|
|
|
Contaminant transport in karst environment and tracer tests in karst system near Turnov
Malechová, Kateřina ; Slavík, Martin (advisor) ; Weiss, Tomáš (referee)
Karst areas are an important source of drinking water but they are at significant risk of contamination. The theoretical part of the thesis focuses on different types of contaminants, their sources and their ways of transport in karst areas. Due to the high velocity of water flow in karst areas, most contaminants are transported rapidly and spread over long distances. Therefore, it is necessary to actively address the issue of contamination in karst environments, focusing mainly on contamination prevention and water flow studies. In the practical part of the thesis, a quantitative tracer test was carried out in the karst system near Turnov using NaCl as a tracer. The measurements confirmed a high water flow velocity of several kilometers per day and the dominance of advection as the main transport process. These results are consistent with previous research. The connection between the sinking stream Šálení smyslů and the Slapy and Bezednice springs was also reconfirmed, with a total recovery of around 50%. This implies that water from the Šálení smyslů most likely flows to other, yet unknown, locations. No communication with the Bartošova pec cave or with the springs used as a source for the local brewery has been demonstrated, so the question remains where all the water from the sinking stream...
|
|
|
Push: Translation and Stylistic Analysis of Selected Passages From the Novel
Hašková, Nikol ; Ženíšek, Jakub (advisor) ; Lancová, Klára (referee)
This bachelor's thesis aims to explore the unconventional register used in Sapphire's novel PUSH. The practical section of the thesis is dedicated to translating the first chapter and the final two paragraphs of the novel from the English language into the Czech language. The theoretical section of the thesis focuses on stylistic analysis of the translation, examining various textual elements such as register, vulgarisms, idiomatic expressions, and place names. The practical section also considers and explains the decisions made during the translation process. The thesis also includes chapters dealing with Sapphire's biography and a brief introduction to translation theory, discussing the theories of Jiří Levý, Dagmar Knittlová, Theodore Savory and Eugene Nida.
|
|
|
Photochemical generation of volatile species of rhodium as an efficient sample introduction technique for inductively coupled plasma mass spectrometry
Hašlová, Karolína ; Musil, Stanislav (advisor) ; Nováková, Eliška (referee)
This master's thesis is focused on development of a new analytical method based on photochemical generation of volatile species (PCVG) of rhodium as an efficient sample introduction technique for inductively coupled plasma mass spectrometry. At first, the conditions affecting the PCVG efficiency were optimized, namely carrier gas flow rate, irradiation time, composition of the reaction medium as well as the impact of transition metal ions as potential sensitizers was examined. The combination of Cu2+ and Co2+ was found optimal, enhancing the PCVG efficiency more than 60-fold. Additionally, a serious effect of the residence time of volatile species in the reaction medium, after exiting the irradiated part of the photoreactor, was found. The PCVG efficiency was doubled by modifying the output part of the photoreactor and another two-fold increase in the PCVG efficiency was achieved using the addition of nitrate anions to the reaction medium. Under chosen optimal conditions, the limit of detection and quantification were 13 pg dm-3 (3σ, n = 11) and 42 pg dm-3 (10σ, n = 11), respectively, and the PCVG efficiency was determined to be 13.5 ± 0.1%. The repeatability (n = 10) at 20 ng dm-3 was 2.9%. These experiments were followed by a study of interferences commonly occurring in the PCVG methods (chloride...
|
|
|
Translation Automata Applied in Visual Arts
Khrystsiuk, Dziyana ; Klembara, Radovan (referee) ; Meduna, Alexandr (advisor)
Cílem této práce je vytvořit nástroj založený na překladovém automatu, který podporuje umělce poskytnutím strukturovaného přístupu k výtvarnému umění. Výsledkem této práce je webová aplikace dostupná online, která byla vyvinuta s využitím frameworku React. Nástroj implementuje překladové pravidla pomocí celulárního automatu a poskytuje umělcům různé kreativní transformace, které jim umožňují představit si potenciální změny jejich stávajících děl a objevit nové možnosti postprodukčních úprav obrazů.
|
|
|
Developing Modular Information System with Domain-Driven Design
Škrášek, Michael ; Rychlý, Marek (referee) ; Burget, Radek (advisor)
Tato diplomová práce se zabývá monolitickými architekturami a doménově řízeným návrhem (angl. Domain-Driven Design) a jejich kombinací při vývoji modulárních informačních systémů. Poskytuje ucelený přehled principů doménově řízeného návrhu a různých monolitických architektur, včetně čisté architektury a modulární monolitické architektury. Poté demonstruje použití těchto vzorů a architektur na ukázkové aplikaci. Práce nabízí pohled na vývoj informačních systémů s využitím populárních přístupů, jako jsou CQRS, čistá architecktura, doménově řízený přístup, modulární monolit a další.
|
guest ::
