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The Magnitude of Governance in Addressing HIV/AIDS Problems in Ethiopia
Balcha, Daniel Messele ; Potůček, Martin (advisor) ; Frič, Pavol (referee) ; Kumsa, Alemayehu (referee)
This case study seeks to understand the scope of governance in addressing HIV/AIDS issues in Ethiopia by making use of HIV/AIDS partnership forums that exist in the country. All individuals representing the government sector, the NGO sector, the for profit sector and HIV/AIDS Prevention and Control Office (HAPCO) both at the national level and in the three regions namely Oromia Region, Southern Nations, Nationalities, and Peoples' Region (SNNPR) and Addis Ababa have been interviewed. Additional data were gathered using a PARTNER tool survey and also relevant documents have been analyzed. The social capital and governance theories create a framework where actors enjoy partnerships and use the benefits in both policy making and implementation. With the help of a problem tree and thematic analysis the study describes the causal effect relationship of the factors affecting partnerships and their ultimate consequence in maximizing social capital and governance. Accordingly, the thesis identifies the specific effects of the 2009 law on partnership endeavors in particular and HIV/AIDS policy implementation in general. Moreover, the study points out the existence of insufficient partnership in both decision making and policy implementation.
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Selected Bacterial and Viral Infections Transmitted through Sexual Contact
Tesařová, Kateřina ; Pavlasová, Lenka (advisor) ; Ehler, Edvard (referee)
Selected bacterial and viral infections transmitted through sexual contact The abstract This thesis deals with selected bacterial and viral venereal disease. It is an STD (Sexually Transmitted Diseases) transmitted mainly through sexual contact. The elaborated retrieval work deals with the occurrence of these selected infections - chlamydia, gonorrhea, chancroid, syphilis, hepatitis B, AIDS, genital herpes, genital warts and cervical cancer. Its task is a summary of the issue of sexually transmitted diseases, a detailed introduction to current information and results of scientific research. The attention is paid to the individual diseases, the general characteristics of viruses or bacteria, symptoms and mode of transmission. Further the study points to ways of diagnosis and treatment, epidemiological incidence of infection. Last but not least the emphasis is on prevention. Keywords: sexually transmitted disease, chlamydia, gonorrhea, chancroid, syphilis, hepatitis B, HIV / AIDS, genital herpes, genital wals, cervical cancer
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Analysis of the retinal nerve fiber layer in hiv positive patients in era of combination antiretroviral treatment
Kožner, Pavel ; Filouš, Aleš (advisor) ; Kalvodová, Bohdana (referee) ; Sedláček, Dalibor (referee)
The aim of the study was to evaluate the effect of human immunodeficiency virus infection (HIV) and antiretroviral treatment on the retinal nerve fibre layer (RNFL). The RNFL hickness defined by standard parameters(TSNIT average, Superior average and Inferior average) was assessed in 48 HIV positive patients using scanning laser polarimeter, GDx VCC device. Results were compared to normal values and tested against factors suspected to affect the RNFL thickness. The mean values of the RNFL standard parameters were for TSNIT average, Superior average and Inferior average, 57,65 ± 6,18 m, 69,38 ± 8,34 m, 68,89 ± 9,50 m respectively, in our cohort. The RNFL thinning was not confirmed in our HIV positive group compared to values on healthy population. No significant correlation between the RNFL thickness and the immune profile or antiretroviral therapy was detected. However, a significant negative correlation between the RNFL thickness with increasing duration of HIV infection was foundin our study that is hypothesized to be possibly on an immune pathological basis. Powered by TCPDF (www.tcpdf.org)
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Optimizing of the regime of marker's examination of clinically important infections in blood donors
Dušková, Daniela ; Tesař, Vladimír (advisor) ; Borovanský, Jan (referee) ; Průša, Richard (referee)
Project title: Optimalization of the regime of marker's examination of clinically important infections in blood donors Project author: Daniela Dušková, M.D. Project supervisor: prof. Vladimír Tesař, M.D., Dr.Sc., MBA, FASN The aim of this project is to contribute to the discussion about introducing the methods of molecular biology into the routine blood donor testing in the transfusions departments in the Czech Republic. The theoretical part includes a brief history and some turning points in transfusion medicine. The next part within the theoretical section is dedicated to the problems of infectious diseases concerning transfusion and the general examination processes used during the selection of blood donors. The end of the theoretical part concentrates on existing possibilities of markers' examination of clinically important infections in blood donors, including the list of processes performed in the Czech Republic, the European Union and other countries. The practical part describes this study, ie. the routine screening test of blood donors using the CMIA method (a routine method) and using RT-Real Time PCR method (a molecular biology method) for detecting infectious markers (HCV, HBV, HIV). Within this part, the principle of both methods and the process of actual examinations are described in...
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Retrocyclins
Soukup, Jakub ; Španielová, Hana (advisor) ; Beranová, Jana (referee)
Defensins are peptides with antimicrobial, antifungal and antiviral activity. Defensins are likely to occur in all vertebrate species. There are three subfamilies of defensins - α, β and θ-defensins. The α-defensins exists only in mammals and θ-defensins were described only in macaques and baboons but their genes occur in genomes of all old world monkeys except Homininae subfamily. In humans no θ-defensins are produces due to premature stop codon in their coding sequence. However, they were produced synthetically in vitro and named retrocyclins. Retrocyclins not only maintain antimicrobial activity - but also exhibit unusual antiviral activity. Retrocyclins successfully protect cells from HIV, HSV, influenza and dengue fever virus infections with no detectable cytotoxicity to host cells. Retrocyclin RC- 101 is close to the clinical testing as microbicide to prevent heterosexual transmission of HIV-1.
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Study of the cleavage kinetics of Gag polyprotein from HIV-1 virus by the viral proteinase
Krištofičová, Ivica ; Ingr, Marek (advisor) ; Martínek, Václav (referee)
Gag polyprotein is the precursor of HIV-1 structural proteins, required for correct assembly, budding and maturation of viral particle within HIV-1 life cycle. The process of maturation into an infectious virion is dependent on Gag and GagPol cleavage at nine predefined sites by HIV-1 proteinase. Its disruption is one of the main targets of HIV treatment. HIV-1, however, develops resistance to the proteinase inhibitors by creating mutations in both the proteinase and the substrate. The Gag processing by HIV-1 proteinase is a highly sequential process, that happens in specific order and rate. Previous biochemical studies determined the kinetic data of these processes using oligopeptides representing naturally occuring cleavage sites. This thesis describes the cleavage of the Gag polyprotein itself, which is the natural substrate of HIV-1 proteinase. For this purpose, the full-length Gag polyprotein was recombinantly prepared in bacterial expression system. The cleavage was carried out and its products were analyzed via SDS-PAGE and Western blotting. The substrate specificity of the wild-type and mutant HIV-1 proteinase with respect to the full-length wild-type Gag polyprotein was compared. Substantial differences were observed between the rates of individual steps of cleavage by the wild-type and mutant...
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MHC and KIR genotyping of macaques in HIV infection research
Matula, Jan ; Maděránková, Denisa (referee) ; Sedlář, Karel (advisor)
Modern research of viral diseases relies on genomic data processing. Not only is the sequence of a virus important, genomic sequence of specific receptors in affected organisms also plays an important role. In this paper, a novel package for processing of next generation sequencing data in infectious disease written using R/Bioconductor language is proposed. Functionality of the package, including implementation of advanced SSAHA algorithm for fast database searches, is demonstrated using genotyping of genes for MHC and KIR receptors of HIV positive macaques.
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Development of mortality in Sub-Saharan Africa during the period 1990-2009
Kvapil, Ondřej ; Hulíková Tesárková, Klára (advisor) ; Burcin, Boris (referee)
The aim of this study was to compare the development of mortality level within individual phases of human life during the period 1990-2009 in selected countries of Sub-Saharan Africa. Two groups of countries were compared. The first group included countries only marginally afflicted with HIV/AIDS disease. The second group contained populations with higher proportion of people with HIV infection. Differences between the two groups were revealed in areas of age-specific mortality rates and various indicators of probability of dying within individual phases of human life. Differences were apparent in all examined areas (child, adult and overall mortality). Attention was also paid to causes of death. In this part of analysis, a different mortality structure by cause was observed between the representatives of both groups of countries.
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