Methods

Methods 636 records found  previous11 - 20nextend  jump to record: Search took 0.00 seconds. 
Resynthesis of oilseed rape from Brassica rapa and Brassica oleracea
Klíma, Miroslav ; Hilgert-Delgado, Alois ; Ulvrová, Tereza ; Bryxová, Pavla ; Vítámvás, Pavel ; Prášil, Ilja Tom ; Kosová, Klára
In order to extend the variability of the genofond, so-called resynthesis can be used, when new genotypes are created by crossing selected Brassica rapa and B. oleracea, by early isolation of immature embryos from young eggs and their in vitro fertilization (Hilgert-Delgado et al., 2015). It has been shown that resyntetized genotypes are distinct from rape materials grown at present (Sosnowska et al., 2010), so that they can serve as a source of new variability in breeding programs focusing on cytoplasmic and pollen sterility, resistance to pathogens and tolerance to stresses (Warwick et al., 2009). Representatives of the initial components are characterized by wide variability in terms of morphology and agronomic characteristics, and are a prospective source of heterosis and other traits (Wu et al. The use of resyntetized genotypes for the direct production of rapeseed varieties is not possible due to the poor quality of the seed of these materials. It is mainly the high content of glucosinolates and erucic acid, typical of most of the initial components (Jesske et al., 2013). However, the required properties can be transferred by crossing to conventional breeding materials (Hilgert-Delgado et al., 2017).
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Determination of the frost tolerance of rapeseed varieties in early stages of plant development
Prášil, Ilja Tom ; Klíma, Miroslav ; Musilová, Jana ; Kosová, Klára ; Vítámvás, Pavel
The methodology is focused on the determination of frost tolerance of varieties, breeding materials and gene sources of rapeseed in the early stages of plant development using a direct frost test. Rapeseed plants grow in pots located outdoors under natural conditions. The sowing of germinated rapeseed seeds is carried out during October so that until the end of November the plants reach the first leaf stage. The plants are exposed to a frost test in pots from December to February according to the described procedures.
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Methodology for long-term evaluation of transgenic plum cv. ´HoneySweet´ resistance against Plum pox virus: Certified methodology applied
Polák, Jaroslav ; Komínek, Petr ; Krška, Boris ; Jarošová, Jana
Methodology describes procedure of establishing the field trial with Biotech plum cv. ´HoneySweet´, growing the plants in the experimental orchard and evaluation of plum resistance against Plum pox virus infection, as well as against co-infection with other two viruses, Apple chlorotic leafspot virus and Prune dwarf virus.
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Farming practices for efficient use of nitrogen and reduction of its losses: Certified methodology for practice
Klír, Jan ; Haberle, Jan ; Růžek, Pavel ; Šimon, Tomáš ; Svoboda, Pavel
The methodology describes the main risks of nitrogen losses and recommended practices for their reduction. Practically applicable methods for the evaluation of the nitrate leaching risk depending on the soil, crop, root range, croping system, irrigation and weather conditions are described in detail. Consequently, suitable soil treatments and fertilization are proposed to improve the utilization of the nitrogen and uniformity of the water soaking.
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Methodology for detection identification of fungi responsible for the cereal contamination with ochratoxin A and citrinin using PCR: Methodology for practice
Salava, Jaroslav ; Novotný, David
The aim of this methodology was to describe a protocol for the rapid and reliable detection of microscopic fungi from the genusPenicillium and Aspergillus capable of producing mycotoxins ochratoxin A and citrinin in cereals, preferably prior to their production and accumulation, using polymerase chain reaction (PCR) and their use, e.g. for the evaluation of health and hygienic safety of plant products.
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Short-term rainfall for hydrological modeling and design of small water management structures in the landscape
Kavka, P. ; Müller, Miloslav ; Strouhal, L. ; Kašpar, Marek ; Bližňák, Vojtěch ; Landa, M. ; Weyskrabová, L. ; Pavel, M. ; Dostál, T.
The aim of this methodology is to reduce the current knowledge deficit in the field of short-term rainfall variability and its application in hydrological modeling and design of small water structures and conservation measures within the catchment. The methodology defines six generalized short-term rainfall scenarios with specific temporal distribution of rainfall intensity. Next their variability related to their occurence frequency and spatial distribution accross Czech Republic is presented. These scenarios were derived from the 10-years time series of radar rainfall measurements adjusted with the daily totals from ground gauging network.
Your guide to the Digital Defenders
Kirsten Fiedler, EDRi ; Theresia Reinhold, EDRi
Brožura s praktickými radami na online ochranu soukromí pro děti.
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GDPR pro společenství vlastníků jednotek a bytová družstva
Iuridicum Remedium
Příručka se vzory dokumentů pro SVJ a bytová družstva, která řeší implementaci Obecného nařízení o ochraně osobních údajů (tzv. GDPR).
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Spatial heterogeneity of selected soil properties assessed from airborne imaging spectroscopy and satellite imagery data
Zemek, František ; Pikl, Miroslav ; Lukas, Vojtěch ; Ždímal, V. ; Kraus, M. ; Širůček, P.
The methodology aims at presenting new and economically efficient approaches to assessment of spatial heterogeneity of selected properties of bare soils. There are three key features the authors paid attention: 1/the simplicity in usage with keeping sufficient accuracy, 2/data availability, 3/economic aspects allowing application to many users. The methodology covers two complementary parts. The first one gives an overview of laboratory and field soil spectroscopy. It is followed by a detail description of practical application of airborne hyperspectral and satellite multispectral data in assessment of spatial heterogeneity of bare soils. All applied methods have been developed and tested on scale of medium size farm.
Management plan of small water supply reservoirs of Landštejn type
Halačka, Karel ; Adámek, Zdeněk ; Jurajda, Pavel ; Mareš, J. ; Kopp, R. ; Grmela, J.
The aim of the methodology is to provide managers and users of small water reservoirs Landštejn type with methodological tools for optimization of the water quality monitoring based on the current level of knowledge and research. Furthermore, provides a design of a methodology for fish community monitoring and optimization of treatments to reduce unwanted fish species and strengthen the status of suitable fish species with the aim of achieving the topdown effect and maintaining or improving water quality in these water reservoirs. The presented methodology summarizes the findings obtained during the monitoring and evaluation of selected parameters of water quality, planktonic and fish communities in water supply reservoirs Landštejn in recent years. It also contains an evaluation of applied treatments, focused mainly on fish stock. Unwanted fish species were caught and the predatory fish were stocked with the aim of optimizing the fish community composition and positively influencing the quality of the water in the reservoirs. The methodology contains recommendations for monitoring of the water quality in the reservoir, including monitoring of planktonic communities, monitoring of fish and recommended manipulation with fish stock. The methodology provides guidance and information for optimization of monitoring and treatments for small water supply reservoirs of the Landštejn type.

Methods : 636 records found   previous11 - 20nextend  jump to record:
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