Mikrobiologický ústav

Nejnovější přírůstky:
2018-01-11
18:41
Development and optimization of the production of recombinant epimerases for pharmaceutical industry. Scale-up of a submerged cultivation technology and down-stream processing
Prell, Aleš ; Kujan, Petr ; Sobotka, Miroslav ; Šafář, Hynek ; Vavruška, Tomáš
Development and optimization of the production of recombinant epimerases for pharmaceutical industry. Scale-up of a submerged cultivation technology and down-stream processing.

Úplný záznam
2017-12-20
13:37
Development of the cultivation of the pathogenic bacteria.
Prell, Aleš ; Kujan, Petr ; Sobotka, Miroslav ; Šafář, Hynek ; Vavruška, Tomáš
Development of the cultivation of pathogenic bacteria with a specific lipopolysaccharide content in the cell structures. Optimization of media and yields.\n\n

Úplný záznam
2017-12-20
13:37
Optimalizace výtěžnosti kultivačního a izolačního procesu přípravy kvasinek
Prell, Aleš ; Kujan, Petr ; Sobotka, Miroslav ; Šafář, Hynek ; Vavruška, Tomáš
Optimalizace růstových podmínek pro různé kmeny vinných kvasinek s ohledem na výtěžnost a vývoj DSP procesů s maximalizací vitality preparátů.\n\n\n\n

Úplný záznam
2017-12-20
13:37
Optimalizace kultivace hlenky Schizochytrium spp. v bioreaktoru.
Prell, Aleš ; Kujan, Petr ; Sobotka, Miroslav ; Šafář, Hynek ; Vavruška, Tomáš
Optimalizace média pro kultivace hlenky produkující DHA. Vývoj procesu, hledání optimálních kultivačních podmínek.

Úplný záznam
2017-12-07
15:36
Optimalizace a příprava výroby podpůrných přípravků pro ošetření slunečnice
Kujan, Petr ; Sobotka, Miroslav ; Šafář, Hynek ; Prell, Aleš ; Vavruška, Tomáš
Příprava výroby a optimalizace výroby zemědělských pomocných přípravků pro podporu pěstování slunečnice

Úplný záznam
2017-08-02
12:52
Scale-up and production of various recombinant oxidoreductases
Prell, Aleš ; Kujan, Petr ; Sobotka, Miroslav ; Šafář, Hynek
Development and optimization of pilot cultivations and DSP of recombinant intracellular oxidoreductases in microbial productions for a food industrial use

Úplný záznam
2017-08-02
12:52
Inaktivovaná bakteriální biomasa pro přípravu lyzátů
Prell, Aleš ; Kujan, Petr ; Sobotka, Miroslav ; Šafář, Hynek ; Vavruška, Tomáš
Inaktivované bakteriální biomasy pro přípravu potravinových doplňků - optimalizace kultivací zdrojových kmenů.\n\n

Úplný záznam
2017-08-02
12:52
Development of a fed-batch technology of the yeast P. pastoris recombinant protein production in a small pilot scale. Optimization of the process and preparation of a scale-up
Prell, Aleš ; Kujan, Petr ; Sobotka, Miroslav ; Šafář, Hynek
Development of a fed-batch technology of the yeast P. pastoris recombinant protein production in a small pilot scale. Optimization of the process and preparation of a scale-up.

Úplný záznam
2017-06-12
14:36
UNICELLULAR ALGA CHLORELLA AS A FEED SUPPLEMENT, CURRENT EXPERIENCE AND FUTURE PERSPECTIVES
Kotrbáček, V. ; Doucha, Jiří ; Kuchařová, V. ; Doubek, J.
We studied the effect of Chlorella biomass as a feed supplement. In laying hens we found that diet supplemented with 1 % (i.e. 10 g/kg) of dry disintegrated heterotrophically grown Chlorella biomass significantly increased content of carotenoids in egg yolks of 8 experimental animals already after the first week of supplementation (P<0,01). Compared to 8 control hens these differences remained significantly higher until the end of the eight-week experiment. The average carotenoid concentration was 17,3 mu g per g of yolk in control group and 25,3 mu g per g of yolk in experimental animals (P<0,01). Autotrophically grown Chlorella was supplemented to the 30 gilts one month prior the expected first heat in a dose of 1,5 g per animal per day. These sows entered heat earlier and the number of pregnancy was higher in comparison to the 30 control animals. Other studies observed digestibility of selenium organically bound in Se-enriched Chlorella. The level of selenium in blood, colostrum and also tissues of new born piglets was determined. Group of 8 sows was supplemented with Se-enriched alga during the pregnancy and 3 days after parturition at a dose of 0,3 mg of selenium per 1 kg of feed. The same amount of selenium in the form of sodium selenite was administrated to the control group of 8 individuals. Organic form of selenium significantly increased concentration of Se in blood of sows and colostrum (P<0,05). Piglets born to supplemented sows had also significantly higher level of Se in heart, muscles and liver (P<0.01). The use of fresh Chlorella biomass produced outdoors on a dairy farm was experimentally tested. The liquid fresh biomass applied to dairy cows during the pregnancy at a dose of 15 g dry mass per cow increased the dry matter content in colostrum from 17,5 g per 100 ml of colostrum in control group to 20,5 g per 100 ml of colostrum in supplemented cows (P<0,05).

Úplný záznam
2017-03-17
18:38
BIOGENIC AND SYNTHETIC AMORPHOUS SILICA - COMPARE AND INTERACTION WITH BACTERIAL SYSTEMS
Fijalkowski, M. ; Adach, K. ; Marešová, Helena ; Kroisová, D.
The aim of the work is to compare the biogenic silica nanoparticles, which have been isolated from rice husks with synthetically produced silica brand Cab-O-Sil LM-150. The comparison is based on an evaluation of the two systems from the point of view of chemical composition, particle size and structure, ability to form clusters of particles and interactions with selected bacterial systems. Methods used for comparison were: SEM, EDX, TEM, FTIR, ICPOES and bacteriological tests. Husks are standardly contaminated by accompanying ions that are important for plant growth, but undesirable for obtaining a quality product. Rice husk used for the silica isolation were purified by boiling in 10% HCl for 2 hours. Rice husks were then washed with distilled water until pH 7. The dried and purified husks were burned in an oven at 650 degrees C of ramping temperature 10 degrees C/min during 2 hours. The obtained product has a chemical composition analogous to the synthetic product. Biogenic particles reach a size of about 20 nm, about 10 nm synthetic. Both materials have an amorphous structure. Interaction with bacterial systems was performed with Gram-negative bacteria Escherichia coli W3110 strain and Gram-positive bacteria Bacillus cereus. In both cases analogous behaviour was observed depending on the concentration of nanoparticles and reducing the growth rate of cultures of 8% for E. coli and 5% for B. cereus resulted nanoparticles at the concentration of 150 mg/litter of medium.

Úplný záznam