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Emergent properties of the G1/S network
Dražková, Jana ; Tomášek, Petr (referee) ; Palumbo,, Pasquale (advisor)
Tato práce se zabývá buněčným cyklem kvasinky Saccgaromyces cerevisiae. Oblastí našeho zájmu je přechod mezi G1 a S fází, kde je naším cílem identifikovat velikosti buňky v době počátku DNA replikace. Nejprve se věnujeme nedávno publikovanému matematickému modelu, který popisuje mechanismy vedoucí k S fázi. Práce poskytuje detailní popis tohoto modelu, stejně jako časový průběh některých důležitých proteinů či jejich sloučenin. Dále se zabýváme pravděpodobnostním modelem aktivace replikačních počátků DNA. Nově uvažujeme vliv šíření DNA replikace mezi sousedícími počátky a analyzujeme jeho důsledky. Poskytujeme také senzitivní analýzu kritické velikosti buňky vzhledem ke konstantám popisujícím dynamiku reakcí v modelu G1/S přechodu.
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The system of treatment of juvenile offenders in Czech Republic
Sváčková, Stanislava ; Koťa, Jaroslav (referee) ; Lorenzová, Jitka (advisor)
Criminality is very compelling phenomenon, which every advanced society struggle with. The adolescent period is less resistant of negative influence society and that is the reason find systém of suitable actions from juvenilie delinquency. This essay handle how is in Czech Republic solving system of treatment of jevenile offenders and what contains. Outline the problematic of adolescent period and system of criminality prevention. Connection with new principle about juvenile justice enlarge criminal process and available deportment solving of criminal behavior of juvenile offender. We can also look through of violation of law and possibility of intervention social curator. Then it will take us through the system of institutions for court-ordered institutional and protective care and system of treatment of juvenile in execution of a punishment. Own space has Probation and Mediation Service because without it will not be able to get one of most important goals, which is maximum to use legal way how to avoid court. The essay ends with sense that system of treatment of juvenile offenders is set up well, but in work experience we can still find low sites. So the space is still open for deeper analysis each of system elements. The analysis sould help to increase efectivity of system like complex.
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Romanesque galleried churches in Bohemia and their founders
Gája, Robert ; Doležalová, Eva (referee) ; Žemlička, Josef (advisor)
In the preceding chapters we examined the relationship between the church and its founder from several perspectives. Various forms of the relationship changed over the 13th and 14th centuries, however their content persisted. Patrons' claims expressed themselves in various domains. A feudal claim on the church property also resulted from the ownership of land itself. As a matter of fact, a whole set of founder's rights is concerned. The public function of churches was not impaired thereby. It did not consist in the realm of property, but only resulted from the parochial function, which the founders did not intend to impair by any means. Ecclesiastical supervision over priests' prebends and the church property did not constitute a transition of this property to ecclesiastical ownership in today's connotation, since the Church as a private legal subject did not exist in the Middle Ages. Nor the founders were the owners to all intents and purposes. The property belonging to the church or prebend was a foundation. Its purpose was to ensure the continuous operation of the church and parish administration, which mattered to both sides, the establishment and the Church. The status of the prebend and the church was to stay the same over the time regardless of the change of priests or landowners. The patron claimed...
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Analysis of horse riding tourism and its infrastructure in the Central Bohemia region
Zímová, Barbora ; Jarolímková, Liběna (advisor) ; Herget, Jan (referee)
The aim of this bachelor thesis is to analyze equestrian trails of Central Bohemia Region with its whole infrastructure - from riding stables, where is possible to accommodate with the horse, over tethering sites, restaurants adapted to the stay with horses or other places to have a rest, up to direction signs and information boards. The thesis also contains theoretical information about the horse riding tourism like a form of a rural tourism. There are mentioned improvement proposals of equestrian trails and the the bachelor thesis should first of all help persons interested in the horse riding tourism in the Central Bohemia region.
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Voronoi diagramy koulí v E3
Maňák, Martin ; Zemek, Josef (referee) ; Kolingerová, Ivana (advisor)
Voronoi diagrams (VD) describe spatial relationships among a given set of input sites. The family of VD for a set of points is a well-explored domain and e ective algorithms for their construction exist. Although the family of VD for a set of spheres has been known for many years, properties of these diagrams and algorithms for their construction are a relatively new thing. Their importance grows with the development in the area of molecular biology. The goal of this work is to survey the theory behind VD of spheres, implement one of the existing algorithms for their construction as a library and use the library on a real data, such as proteins.
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Distribution of CD46 and β1 integrin molecules with respect to different membrane structures of the sperm head
Šebková, Nataša ; Frolíková, Michaela ; Děd, Lukáš ; Dvořáková-Hortová, Kateřina
CD46 protein plays an important role during fertilization and its role is associated with acrosome stability. CD46 is probably involved in signalling pathways triggering the acrosome reaction (AR). It also associates through membrane integrins with specific MAP kinases involved in the AR. Our aim was to monitor the dynamics of relocation of CD46 and β1 integrin during sperm maturation and its preparation for the fertilization. The dependence of this localization changes on the dynamic of actin cytoskeleton was studied. Our results show the changes in the localization of these proteins associated with the AR and their co-localization was observed using proximity ligation assay. After the AR CD46 and β1 integrin spreads across the sperm head, entering the post-acrosomal compartment, and permeates the borders of different domains. It was shown previously that actin dynamics is necessary for acrosome reaction-associated translocation of Izumo1 protein that is required for sperm-egg fusion. Therefore Latrunculin A was used during sperm incubation. The co-incubation of capacitated sperm with Latrunculin A leads to a decrease of the percentages of sperm, which express relocation pattern after induced AR. 3D models and visualizations of potential membrane processes responsible for the relocation of proteins from the acrosomal area to the other compartments of the sperm head were prepared. Our results deliver new information that proteins CD46 and β1 integrin undergo dynamic relocation towards the sites of sperm-egg fusion during the AR in vitro. The inhibitor of actin dynamics abrogates significantly the AR-associated changes in proteins localization. We speculate that this relocation is of importance for the successful sperm-egg interaction, adhesion and subsequent gamete fusion.
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Cultural uses of former cinema venues
Švecová, Aneta ; Čuříková, Tamara (advisor) ; Pittermann, Jiří (referee)
Diploma thesis is dealing with transformation of former classic cinemas in Prague for another kind of cultural use. It is paying attention to the problems of decline of venues which for a long time used to present principal scenes of Prague cultural life. Thesis became an unique material of its kind, dealing with the particular cinemas development til the present day. Cinemas transformation into present theatres, music clubs, dance studios etc maintains the tradition of these venues as places of interest for people who are searching for cultural experience, education and entertaining. One part of thesis consists of examples of former cinema venues which are still waiting to its new use. Well arranged and useful overview of the cinema network development and present situation of Czech cinemas could be found in the introduction.
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Analysis of religious tourism in Italy, focusing particularly on Assisi
Doleželová, Dana ; Netková, Jarmila (advisor) ; Valentová, Jana (referee)
This thesis analyzes the current religious tourism in Italy. It charts the world and the Italian pilgrimage sites and their religious significance. It describes tourism in Italy with a focus on cultural tourism, UNESCO, financing cultural tourism and Italian bureaucracy. The first part describes the different forms of tourism in Italy, methods of promotion of tourism and the benefit of UNESCO. The following chapter is about the phenomenon of pilgrimage, especially its history and different types of motivation. The third chapter describes the care of monuments in Italy. The last part examines tourism in a significant pilgrimage Italian city of Assisi, focusing on some of the economic indicators.
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Residential building Hostivar - construction and technological preparation of construction
Hanáček, Patrik ; Řihák, Pavel (referee) ; Šlanhof, Jiří (advisor)
The theme of the diploma thesis is elaboration of selected parts of the construction technology project in building block of flats Hostivař. Content of this project is technological solution for realization of this project. It means working on time planning of construction, right using of human, material and machinery sources and controlling system for selected part of the building. In attachment of the diploma thesis, there are also drawings of building site equipment for different construction phases. The goal of the diploma thesis is design right and economical procedure for realization of the building, comply with the safety, quality and ecology conditions.
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