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Možný vliv zemětřesení na nízkofrekvenční elektromagnetické vlny: pozorování na družici s nízkou orbitou
Němec, František ; Santolík, Ondřej ; Parrot, M.
We present results of a statistical study of VLF (up to 10 kHz) wave intensity measured by a low-altitude spacecraft in the vicinity of earthquakes. The data from the French DEMETER spacecraft (altitude about 700 km, nearly Sun-synchronous orbit) that was specially developed for such kind of studies are used. The recently developed two-step data processing method is briefly introduced and the main obtained results are reviewed. Finally, a simple independent test of the obtained results is done in order to give an additional evidence for the existence of possible seismic-related effect. Our results indicate that there is a decrease of power spectral density of electric field fluctuations shortly (0-4 hours) before the time of the main shock. This decrease was observed close to surface earthquakes (depth less than 40 km) with magnitude larger than or equal to 4.8 and only during the night. No similar effect was observed for deep earthquakes nor during the day.
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Optimization of Manufacturing and Assembling Technology of the Valve Bolt
Hrouzková, Andrea ; Zapletal, Josef (referee) ; Doležal, Pavel (advisor)
The thesis aimed to study the impact of manufacturing and assembly technology, the valve bolt fitted to diesel high pressure pump CP3 in the Bosch, which has been found to leak. As experimental material was chosen heat treated bolts M16x1,5 made of material 12 050th. Groups of samples were produced in two ranges hardness. Experimental determination of the optimum range of hardness was assessed on the basis of the results of the process of the screw and set the value of plastic deformation of the sealing surfaces of the parts. Experimental section also deals with the analysis of bearing surfaces on the pump and the components, after the screw process. To some extent, is observed as surface treatment
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: Investigation of lymphocyte populations in the porcine small intestine
Kárová, Kristýna ; Šinkora, Marek (advisor) ; Javorková, Eliška (referee)
8 ABSTRACT Historically pig is allocated to a group of animals which use certain parts of their small intestine to acquire a fully developed primary B cell reperoire. Development of such primary repertoire is independent on the antigen presence and resembles the primary lymphopoietic activity of avian bursa of Fabricius. However, some findings concernig the pig's alignment in the above mentioned group suggest otherwise. This graduation thesis is focused on the investigtion of lymphocyte populations and subpopulations in the small intestine of germ-free and conventional piglets. The aim is to determine whether the percentage amounts of lymphocyte populations is dependent on the intestinal colonization. Using Flow Cytometry the significant differences between individual samples were assesed allowing us to conclude which parts of the small intestine could possibly be used for the development of B cell repertoire. Moreover, the status of isotype switching of B lymphocytes isolated from different intestinal parts was determined by the means of PCR analysis. Our data suggest that the small intestine colonization has a crucial role in development of all the main lymphocyte populations as well as some of their subpopulations. The greatest influence of colonization was observed concerning B lymphocytes and their...
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To the communication of the towns in the period before the Battle of the White Mount (The correspondence among New Town of Prague and some king's towns in the central part of Polabí)
Vojtíšková, Jana ; Hlaváček, Ivan (advisor) ; Ryantová, Marie (referee) ; Ledvinka, Václav (referee)
The submitted thesis aims to scan the written communication among the city councils of royal towns in the period before the Battle of the White Mount, particularly the correspondence among the New Town of Prague and Royal Towns in the Central Part of Polabí, explicitly Český Brod, Kolín, Kouřim, Kutná Hora and Nymburk. The research was based mainly on 20 town registers of the period and on the extant scripts which enabled me to set up both the communication process and particular agents. Thus we were offered the insight into the townhall, its offices, circumstances of the origin of missivs, we observed individual characters and institutions involved in the negotiation procedures. We came across the issue of messenger service mediating the delivery and last but not least we were concerned about receiving correspondence, its interpretation at the time and the content of discussed matters. Another issue we dealt with was the terminology ie. definition of written communication, diplomacy terminology, especially register issue defined as supplemental office book in publishers'offices containing the office scripts. The written communication of the perion represents just one component of wide range of ties occuring in the corporate society. The most significant role was represented by the city council the...
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Study of physiology and searching of proteomic tools useful in suppression and detection of Varroa destructor
Holenková, Martina ; Erban, Tomáš (advisor) ; Titěra, Dalibor (referee)
This work is focused on the study of physiology and proteome of the the mite Varroa destructor and on comparison with the honeybee Apis mellifera. Varroa is currently a major problem for beekeeping, because infects most of the colonies. The control of the mite can not be done without residues both in the hive and for example in the honey or other resources used by the man. Another problem can also be the simultaneously discussed issue of the connection with the Colony Collapse Disorder. The internal anatomy of V. destructor was studied by using paraffin histology. On histological sections stained using hematoxylin and PAS was possible to observe the mite digestive system, but also egg, ovaries or brain. The primary aim of this study was to identify the proteins of mite V. destructor and bee A. mellifera as a host of this parasite. For the electrophoretic separation was used two- dimensional gel electrophoresis, where the second dimension was carried out using 12% and 15% SDS-PAGE. The most abundant spots were selected for analysis using MALDI TOF/TOF mass spectrometry. The most abundant protein identified in samples of V. destructor was hexamerin, arginine kinase or hemelipoglycoprotein precursor. Hexamerins were also identified as the major proteins in the pupae samples. On the contrary the main...
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Mitochondrial uncoupling and mitochondrial morphology in relation to type-2 diabetes
Špaček, Tomáš ; Ježek, Petr (advisor) ; Kalous, Martin (referee) ; Vařecha, Miroslav (referee)
- 2 - 1 SUMMARY Increased ATP/ADP ratio resulting from enhanced glycolysis and oxidative phosphorylation represents a plausible mechanism controlling the glucose-stimulated insulin secretion (GSIS) in pancreatic -cells. Using high resolution respirometry and parallel mitochondrial membrane potential ( m) monitoring we have quantified bioenergetics in rat insulinoma INS-1E cells representing a suitable model for studies of insulin secretion in vitro. Upon glucose addition to glucose-depleted cells, we have demonstrated a simultaneous increase in respiration and m during GSIS and shown that the endogenous state 3/state 4 respiratory ratio hyperbolically increases with glucose, approaching the maximum oxidative phosphorylation rate at maximum GSIS. Attempting to assess the basis of the "toxic" effect of fatty acids on insulin secretion, GSIS has been studied after linoleic acid addition. The linoleic acid addition diminished the observed respiration increase, m jump, and magnitude of insulin release, and reduced state 3/state 4 dependencies on glucose, which was caused by mitochondrial uncoupling. Energetic status of mitochondria, the rate of oxidative phosphorylation, is expressed not only by bioenergetic parameters measurable by classical biophysical methods (e.g. above mentioned respiration and...
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Visual preferences for wind turbines
Běťáková, Vendula ; Sklenička, Petr (advisor) ; Janečková, Kristina (referee)
This dissertation is a selection of three papers focused on visual preference of wind turbines. Visual impact presents the connectivity of the articles, whereas each one deals with the topic from different perspective. First paper "Wind turbines location: How many and how far?" analyses in detail the characteristics of wind turbines themselves, i.e. their number and distance from observer or vantage point in relation to landscape quality. Second paper "How education orientation affects attitudes toward wind energy and wind farms: implications for the planning process" concentrates on respondents´ characteristics, in particular educational orientation, general attitude, occurrence of WTs in respondents´ vicinity and willingness to live close to WTs. Whereas first two papers use same assessment method to analyse differences of WTs perception from different point of view, the third paper "Futuristic Wind Power Systems Suitable as Artistic Sculptures" presents review of WTs appearance in general and proposals of possible future look as an alternative to traditional wind turbines.
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Problems of Czech morphology in selected handbooks
Prohorenco, Dmitrii ; Hrdlička, Milan (advisor) ; Holub, Jan (referee)
Czech language is inflective, that is one ending/suffix cumulates many categories. Many words (especially nouns, verbs and adjectives) have many forms. There are seven cases, fourteen basic types for nouns, five verb conjugation classes, ten parts of speech in czech. Verbs have categories of voice, aspect, person and tense. Nouns have gramatic categories of number, case and gender. There is a variety of irregularities, dual forms etc. in czech. Czech orthography is complicated when compared to croatian or finish, but is relatively simple when compared to french or english. One can say that czech orthography is phonological, but it takes into consideration also morphology and etymology. Czech is spoken primarily in Czech republic. There are some czech minorities in Australia, Argentina, Romania and USA. Czech is quite popular for foreigners to study, also it is spoken only by aproximately 12 mln. of people. The czech word order is different from the word order of english or french, that is relatively restrictive. The czech word order is on the other hand relatively free. Often all the permutations of words in a clause are possible. The concept of czech word order introduced Vilem Mathesius - the co-founder of Prague Linguistic Circle. Usually there exists the basic word order. Observing discourse patterns,...
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