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Design of mountain bike frame
Haman, Martin ; Lehocký, Peter (referee) ; Zvonek, Miroslav (advisor)
The aim of master thesis is the design of full suspension mountain bike frame for racing and competition use. The base of the design solution is selection optimal conception of suspension which influence external design of the frame. General benefit of this concept is utilizing specific properties of carbon composite for design of funkcional frame parts and their technical and visual integration. Important part of this work is design of frame details: rear ends, rear shox's link and duct of the bowdens, wires and brake hydraulic housing.
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TIB AV-Portal: A Reliable Infrastructure for Scientific Videos
Plank, Margret
The German National Library of Science and Technology provides a web-based platform for quality-tested scientific videos including computer visualizations, experiments, interviews as well as recordings of lectures and conferences. Key feature of the portal is the use of automated video and semantic analyses, which enables pinpoint and cross lingual search on a segment level. All videos are assigned by Digital Object Identifi ers (DOI). As a result videos can be published in a scientifically sound way and be linked via DOIs to other research work like the corresponding article.
Fulltext: idr-1033_4 -  PDF
Slides: idr-1033_2 - PDF; idr-1033_3 - PDF
Video: idr-1033_1 -  MP4
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The roof structure of a multipurpose sport hall in Brno
Grussmann, Jan ; Pilgr,, Milan (referee) ; Šmak, Milan (advisor)
Bachelor´s thesis describes the design and static assessment of the main roof structure supporting elements of the multipurpose sport hall in Brno. It is the emphasis on the optimization of the proposal in terms of material consumption. Curved girders made of glued laminated timber and on the edge connected rafters forms together the cross-link structure. Basis of OSB sheathing carrying the roof deck is secured by the orthogonal grid system purlins and rafters. The static calculation also includes fire resistance reports of single elements.
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Fanconi anemia and pancreatic cancer
Hucl, Tomáš ; Špičák, Julius (advisor) ; Nečas, Emanuel (referee) ; Vodička, Pavel (referee)
Inactivation of the Fanconi anemia (FA) pathway occurs in diverse human tumors including pancreatic cancer and renders those tumors hypersensitive to DNA interstrand-cross-linking agents (ICL). How to treat specificly pancreatic and other cancers harboring FA mutations has recently raised great interest, yet preclinical studies have been hampered by the lack of well-controlled human cancer models. We endogenously disrupted FANCC and FANCG in an adenokarcinoma cell line and observed a typical phenotype of FA pathway deficiency (abrogation of FANCD2 monoubiquitination; chromosomal instability, G2M arrest and decreased proliferation upon treatement with ICL, spontaneous chromosomal breakage). Homozygous deletion was achieved for FANCC and FANCG but not for FANCD2 and BRCA2/FANCD1 in RKO cells, suggesting a detrimental phenotype. It provided direct evidence for the paradoxical assumption that their inactivation could be predominantly selected against in cancer cells. Using high-throughput screening, we assessed the growth of our isogenic FANCC and FANCG cells upon treatment with 880 active drugs and 40 000 diverse compounds. The compound having the stronges effect, named 80136342, had a distinct mechanism of action from that of ICL agents. When applied in combination with ICL agents, 80136342 had at least...
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The Sport Hall in Prostějov
Houška, Daniel ; Štrba, Michal (referee) ; Šmak, Milan (advisor)
The goal of the submitted thesis is a design and assessment of a steel load-bearing structure of a multipurpose sports hall situated in Prostějov. The floor plan measurements of the object are 38x54 m with the maximum ceiling height of 9,5 m. Final design variant was chosen on the basis of two preliminary drafts, both processed according to standard ČSN EN. The structure is designed with respect to ultimate and serviceability limit states. The construction consists of 10 cross-links spaced by 6,0 m. The spatial rigidity of the structure’s main load-bearing system is provided by spatial elliptic trussed girders, longitudinal and sway bracings. Roof cladding is carried by purlins placed on trusses. Steel columns are designed as a part of gable walls. The project was carried out in Scia Engineer 2014 software. Some particular elements were subsequently assessed by means of manual calculation. The thesis also includes assessment of joints, construction details and drawing documentation.
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The estimation of oxidative DNA damage using the single-cell gel electrophoresis (commet assay).
Škubalová, Věra ; Hochmann, Jiří (advisor) ; Slyšková, Jana (referee)
The oxidative cell damage represents one of the most common type of damage, which concerns all cell components. It produces the oxidative modifications of lipid structure, proteins and DNA. We concentrated on the last one in this work. The oxidative changes result in modification of replication cell cycle, which are represented for example by carcinogenesis, mutagenesis or by cell aging. Cells are provided by repairing mechanism. Owing to this mechanism the cells are able to repair this damage and stay viable. The oxidative damage is induced by various factors, for example free oxygen radicals or UV radiation, which we used for producing oxidative damage of DNA in this work. It is possible to measure the oxidative damage by using different methods, the most common way is using the comet assay. It represents an important method used for detection of single and doublestrand breaks, for detection of alcali-labile sites, searching for DNA- DNA/DNAprotein cross-linking, oxidative and alkylate damage of DNA. Only a little number of cells are needed for the measurement, which is the most important advantage of this method. We also used the modification of comet assay. It means the application of the reparative enzymes, which are able to recognize the specific oxidative damage of DNA. We used three...
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The repair of DNA damage induced with suphur mustard and its relationship to the cytotoxicity
Jílková, Martina ; Vopršalová, Marie (advisor) ; Herink, Josef (referee)
The aim of this study was to investigate the induction and repair mechanisms of DNA damage caused by sulphur mustard in specific cell lines and their relationship to cytotoxicity of sulphur mustard. Sulphur mustard is a chemical warfare agent of the blistering agent category, which can be misused in local conflicts, terrorist attacks and during liquidation of its storage. Sulphur mustard is an alkylating agent, which interacts with a wide range of cellular macromolecules including DNA, RNA and proteins. Sulphur mustard forms single - strand breaks, monofunctional guanine and adenine adducts, as well as interstrand cross-links involving the two guanines in interaction with DNA. This study is aimed at cross-links. We used the Single Cell Gel Electrophoresis (SCGE, comet assay) for their detection. It is a method of evaluation of single cells ambeded in agarose. It is used for detection of DNA single strand breaks in a single cell. In our modification of the method we determined cross-links, which make the alkaline DNA unwinding impossible. That is why we had to induce a standard number of single strand breaks in DNA by styreneoxide (at a certain concentration and exposure time) before the comet assay. We studied DNA repair mechanisms of specific DNA lesions using specific cell lines with clearly...
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Multipurpose hall in Holice
Rückerová, Eva ; Hron, Lukáš (referee) ; Šmak, Milan (advisor)
Master´s thesis deals with the design and assessment of the roof construction of the multi-purpose hall in Holice. The other two dimensions of the hall are 28 x 45 m. Height of the hall is 11,28 m. Roofing is done in three variants. The final solution is processed for one presentation. The main supporting element is formed by cross-linking arc beam made of laminated wood. Girders are spaced 5 m and connected purlins. Stability of the construction by three field bracings. The cladding is a rafter, shuttering of OSB particle that carry the roof deck.
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Hereditary and non-hereditary factors affecting starch content in potato tubers
Malá, Lucie ; Sedláková, Vladimíra (advisor) ; Dvořák, Petr (referee)
This literature review relates to the starch which is presented in potato tubers, and hereditary and non-hereditary factors that influence content of the starch. At the beginning of the work, the attention is paid to the potatoes itself, especially their general characteristics. It should also mention the content of substances in tubers and their impact on human health. Further, the work is focused on the main thema, especialy starch present in potato tubers. Starch has considerable importance for the entire national economy, currently used not only in the starch industry, but it is more frequently used in other industries, for example paper or textile industry. Starch is composed from two important polysaccharides, amylose and amylopectin which are described in details. There are factors, that may influence starch content in potato tubers, like genetic and environmental factors. The simplest genetic factor that can influence the content of starch in potato tubers is crossing. The biosynthesis of starch is very important, which depends on several enzymes that initiate the metabolic pathway. It is pyrophosphorylase, starch synthase (SS), enzymes branched starch (SBEs) and enzymes unbranched starch (DBES). Starch synthase plays an important role here, in particular her isoform GBSS (granule bound starch synthase), which synthesizes amylose. Amylopectin is synthesized by a complex of enzymes SSI, SSII, SSIII, SSIV. The manipulation of single sugar genes metabolism can affect the formation and composition of starch in any variety of potatoes. This manipulation is called transgenesis and her goal is the creation of new potato genotypes. We can produce potato starch with high amylose by inhibiting enzymes SBE A and SBE B. A starch with a high amount of amylose is highy asked from the industry, because starch has unique functional properties. It should be mentioned DNA markers that has connection with the cultivation of potatoes and offers new opportunities for selecting potatoes genotypes. With DNA markers is linked MAS analysis and the associated method PCR polymerase chain reaction method. Another important method is QTL method. The environmental factors which influence the starch content of potatoes, which are mentioned in this work, are drought, light, cold. It should also be noted that even postharvest stress has a negative effect on starch content in potato tubers. Environmental stress affects and changes the yield of the starch.
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