Original title:
Study of DNA-protein interactions by cross-link formation using aqueous Michael addition
Authors:
Daďová, Jitka ; Orság, Petr ; Pohl, Radek ; Brázdová, Marie ; Fojta, Miroslav ; Hocek, Michal Document type: Papers Conference/Event: Symposium on Chemistry of Nucleic Acid Components /16./, Český Krumlov (CZ), 2014-06-08 / 2014-06-13
Year:
2014
Language:
eng Abstract:
Acrylamide and vinylsulfonamide were designed as reactive DNA modifications for covalent crosslinking with cysteine containing peptides and proteins. The corresponding functionalized cytidine triphosphates were prepared and enzymatically incorporated into DNA. These modified oligonucleotides were then used for conjugation with DNA binding domain of protein p53.
Keywords:
DNA; Michael addition; nucleic acids Project no.: GA203/09/0317 (CEP), GAP301/11/2076 (CEP) Funding provider: GA ČR, GA ČR Host item entry: Chemistry of Nucleic Acid Components. 16th Symposium, ISBN 978-80-86241-50-0
Institution: Institute of Organic Chemistry and Biochemistry AS ČR
(web)
Document availability information: Fulltext is available at the institute of the Academy of Sciences. Original record: http://hdl.handle.net/11104/0234284