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Detection of signal electrons at higher pressure in the specimen chamber
Jirák, Josef ; Autrata, Rudolf ; Špinka, Jiří
The advantages of the scanning electron microscopy working at higher pressures in the specimen chamber are connected with the possibility of observation of specimens structures, which are difficultly observable without previous preparation for microscopes working with pressures in the specimen chamber under 10.sup.-2./sup. Pa. The pressure in the specimen chamber up to approx. 2000 Pa brings the possibility of observation of specimens, which release gases, specimens containing liquid phase, including wet biological preparations, reactions on the phase interfaces, etc. At higher pressures in the specimen chamber it is also not necessary - due to neutralisation of the surface negative charge by gas ions - to coat electrically non-conductive specimens by a conductive layer.
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Systém odměňování v malém IT podniku
Novotný, Lukáš ; Dvořáková, Zuzana (advisor) ; Cetkovský, Pavel (referee)
The topic of this thesis is reward management and its application in a small-sized IT enterprise. Its goals are to properly document and describe current reward system in the company, evaluate the system and recommend improvements for the system. The first two goals are reached via thorough research of the company through semi-structured interviews, employee satisfaction survey, participant observation and document examination and the last one by applying principles from the current literature and education to the results of the two previous goals. The findings are that the reward system, despite growing organically instead of designed, is relatively effective in some areas, but needs improvement in other areas. The main improvement areas are motivation by contingent pay, communication management and performance management. Relatively well working rewards are especially non-financial reward connected to the work environment and work itself.
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Analysis of the russian investment environment focused on foreign direct investment in the selected countries of BRICS
Tuktamyšev, Eduard ; Gullová, Soňa (advisor) ; Klosová, Anna (referee)
The aim of this Master thesis is to analyze investment environment of the Russian Federation by focusing on foreign direct investment in the selected countries of BRICS cluster. First chapter is dedicated to the theoretical definition of the investment climate, observation methods such as PESTEL and SWOT analysis, indexes of the multinational organisation (Global Competitivness Index, Ease of Doing Business a Index of Economic Freedom). This chapter also defines investment incentives and foreign direct investment together with typology of this two terms. Second chapter focuses on the Russian investment policy especially on legislative and administrative investment regulation. Last part of this chapter focuses on development of special economic zones, which parcipate in alocation of the foreign direct investment in Russia the most. At the end of this thesis there are strenghts and weaknesses of the russian investment environment together with opportunities and threats described.
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Clustering and regression analysis of micro panel data
Sobíšek, Lukáš ; Pecáková, Iva (advisor) ; Komárek, Arnošt (referee) ; Brabec, Marek (referee)
The main purpose of panel studies is to analyze changes in values of studied variables over time. In micro panel research, a large number of elements are periodically observed within the relatively short time period of just a few years. Moreover, the number of repeated measurements is small. This dissertation deals with contemporary approaches to the regression and the clustering analysis of micro panel data. One of the approaches to the micro panel analysis is to use multivariate statistical models originally designed for crosssectional data and modify them in order to take into account the within-subject correlation. The thesis summarizes available tools for the regression analysis of micro panel data. The known and currently used linear mixed effects models for a normally distributed dependent variable are recapitulated. Besides that, new approaches for analysis of a response variable with other than normal distribution are presented. These approaches include the generalized marginal linear model, the generalized linear mixed effects model and the Bayesian modelling approach. In addition to describing the aforementioned models, the paper also includes a brief overview of their implementation in the R software. The difficulty with the regression models adjusted for micro panel data is the ambiguity of their parameters estimation. This thesis proposes a way to improve the estimations through the cluster analysis. For this reason, the thesis also contains a description of methods of the cluster analysis of micro panel data. Because supply of the methods is limited, the main goal of this paper is to devise its own two-step approach for clustering micro panel data. In the first step, the panel data are transformed into a static form using a set of proposed characteristics of dynamics. These characteristics represent different features of time course of the observed variables. In the second step, the elements are clustered by conventional spatial clustering techniques (agglomerative clustering and the C-means partitioning). The clustering is based on a dissimilarity matrix of the values of clustering variables calculated in the first step. Another goal of this paper is to find out whether the suggested procedure leads to an improvement in quality of the regression models for this type of data. By means of a simulation study, the procedure drafted herein is compared to the procedure applied in the kml package of the R software, as well as to the clustering characteristics proposed by Urso (2004). The simulation study demonstrated better results of the proposed combination of clustering variables as compared to the other combinations currently used. A corresponding script written in the R-language represents another benefit of this paper. It is available on the attached CD and it can be used for analyses of readers own micro panel data.
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CRM System Optimization
Fučík, Ivan ; Mildeová, Stanislava (advisor)
This thesis is focused on CRM solutions in small and medium-sized organizations with respect to the quality of their customer relationship. The main goal of this work is to design an optimal CRM solution in the environment of real organization. To achieve this goal it is necessary to understand the theoretical basis of several topics, such as organizations and their relationship with customers, CRM systems, their features and trends. On the basis of these theoretical topics it is possible to analyze current CRM solution in the organization. From the data obtained by the methods of observation and controlled interview it was possible to prepare a design for optimal CRM solution. This optimal solution comes also from user and organization requirements. The optimal CRM solution saves time and costs associated with current CRM solution and replaces existing and ineffective CRM tools.
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Forms of advertising on social networks and consumer protection
Žďárský, Jiří ; Vavrečka, Jan (advisor) ; Štěpánek, Petr (referee)
The theme of this thesis are forms of advertising on social networks and consumer protection. The main informational source is above all research of specialized literature, articles, online or other sources in connection with author's own observations and analysis of the respective legislation and ultimately the comparison with author's gained professional experience. The result of this work is a comprehensive overview and introduction to the law of social networks and advertising, demonstration of the importance and significance of this topic and finally evaluation of the effectiveness of legislation that protects consumers in the environment of social networks.
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Marketing mix of the fitness centre
Choutková, Aneta ; Khelerová, Vladimíra (advisor) ; Vojtíšek, Zdeněk (referee)
The aim of the thesis is to define and evaluate the marketing mix of the fitness centre World Gym Fitness Group Inc. and to recommend changes that would increase the efficiency of the marketing mix. The theoretical part is divided into two chapters and focuses on marketing mix of services and marketing environment. Within the crucial practical part there has been run a research to determine the mission of the company and clients´ satisfaction. The research is based on direct observation. The practical part contains at first general characteristics of the World Gym company. The next chapters are focused on the individual parts of the marketing mix of the fitness centre. The marketing mix includes services, price, promotion, distribution, physical environment and people. At the end all findings are summarized in the SWOT analysis and recommendations are designed to increase the efficiency of the marketing mix of the fitness centre.
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Study of physiology and searching of proteomic tools useful in suppression and detection of Varroa destructor
Holenková, Martina ; Erban, Tomáš (advisor) ; Titěra, Dalibor (referee)
This work is focused on the study of physiology and proteome of the the mite Varroa destructor and on comparison with the honeybee Apis mellifera. Varroa is currently a major problem for beekeeping, because infects most of the colonies. The control of the mite can not be done without residues both in the hive and for example in the honey or other resources used by the man. Another problem can also be the simultaneously discussed issue of the connection with the Colony Collapse Disorder. The internal anatomy of V. destructor was studied by using paraffin histology. On histological sections stained using hematoxylin and PAS was possible to observe the mite digestive system, but also egg, ovaries or brain. The primary aim of this study was to identify the proteins of mite V. destructor and bee A. mellifera as a host of this parasite. For the electrophoretic separation was used two- dimensional gel electrophoresis, where the second dimension was carried out using 12% and 15% SDS-PAGE. The most abundant spots were selected for analysis using MALDI TOF/TOF mass spectrometry. The most abundant protein identified in samples of V. destructor was hexamerin, arginine kinase or hemelipoglycoprotein precursor. Hexamerins were also identified as the major proteins in the pupae samples. On the contrary the main...
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Sorption and Stabilization of Metals/Mettalloids by Innovative Synthesized Sorbent Amochar.
Ouředníček, P. ; Trakal, L. ; Komárek, M. ; Pohořelý, Michael
Remediation of contaminated soil which is based on stabilization and immobilization of potential\nhazardous substance by sorption materials has been studied intensively nowadays. Biochar – activated organic carbon belongs to this group of stabilizing agents which can adsorb wide range of contaminants, including metals/metalloids. Surface area of the biochars is quite large in general and functional groups (e.g. COO–) can form chelates or alkaline elements on the surface, which is represented by cation exchange capacity. Altogether with the high pH values (7.00 – 10.00), biochars are quite effective sorbents and can adsorb metals/metalloids from the solution (ground water), especially in acidic soils (in the environments affected by intensive mining activities). Sorption\neffectiveness can be increased (especially for As (V) or Cr (VI) sorption) by modification of biochar by various types of secondary oxides. Innovative sorbent AMOchar (AMO + biochar) has been synthesized currently. The product was prepared by adding of biochar to the reaction solution during amorphous manganese oxide (AMO) synthesis. The AMOchar was formed mainly by Mn-oxalates which had coated surface of the pristine biochar. AMOchar composite was able to remove significantly higher amounts of various metal(loid)s from the solution despite the rather high pH of the material. Sorption effectiveness was high not only in case of Pb (II) sorption (almost 99%), and Cd(II) (51.2%), but also a very high amount of As(V), 91.4%. Additionally, both AMOchar composite was able to reduce Mn leaching. This can avoid potential post-contamination caused by the dissolution of less stable Mn-oxalates as observed in the pure AMO.
Fulltext: content.csg -  PDF
Plný tet: SKMBT_22316111113040 - PDF
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Information content in vocalization of guinea pigs (Cavia porcellus): individual distinction and recognition of predators
Baklová, Aneta ; Baranyiová, Eva (advisor) ; František, František (referee)
Guinea pigs represent domesticated precocious rodents which became common pets. From the first day after birth they are fully vocal. This thesis was devoted
to the vocalization of pups. The aim of the thesis was to 1) determine the age when the vocal individuality in whistle sound is demonstrable; 2) test possible ultrasonic signals emitted by young guinea pigs; 3) study the antipredator reactions and alarm calls to aerial (bird of prey) and terrestrial (dog) predators and human (control test).
A total of 16 guinea pig pups were tested for vocal individuality, 28 pups
for ultrasonic vocalization and 27 adolescents for predator recognition. When testing vocal ontogeny during the first 9 days after birth, I observed changes in temporal, frequency and parameter of intensity . When I test vocal individuality by cross-validated discriminant function analysis (DFA) based on ten acoustic parameters, calls were classified to correct animals with following success: day 1 = 71.9%, day 3 = 58.8%, day 5 = 53.10%, day 7 = 50.60% and day 9 = 63.10%. The highest frequency in whistle was 30.03 kHz. In predator discrimination reactions as freezing, fleeing and vigilance were observed. In the presence of dog, guinea pigs reacted for the longest time and most frequently by freezing. When confronted with a bird of prey, I observed for a longest time and most often fleeing and then freezing. When exposed to a human, guinea pigs showed mostly vigilance. Almost no vocalization was observed except for two events
of alarm calls - drrr as in the presence of dog and chirrup as reaction to bird of prey.
The following conclusions can be drawn from the presented results: 1) vocal individuality of guinea pigs is demonstrable immediately after birth and the rate
of individually different vocal parameters changes with age; 2) guinea pigs are able
to produce sound up to 30 kHz, i.e. within the ultrasound range, but signals of high frequencies are not crucial for their communication; 3) guinea pigs discriminate between terrestrial and aerial predators, but they emit alarm calls rather rarely.
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