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Sensory evaluation of drinks enriched with extracts of herbs
Scholzová, Kristýna ; Pořízka, Jaromír (referee) ; Vítová, Eva (advisor)
This diploma thesis deals with sensory evaluation of herbal non-alcoholic beverages, being a part of a new product development. The theoretical part provides information concerning chemical composition, health effects, food applications and aroma compounds of the herbs of interest (peppermint (Mentha piperita), sage (Salvia officinalis), St. John’s wort (Hypericum perforatum) and lemon balm (Melissa officinalis). Another topic is a description of sensory methods, requirements and arrangement of the analysis, statistical methods, and also technological processes leading to herbal syrups and beverages production. The practical part comprises sensory analysis of a few, newly designed, formulas of one-component and two-component alcohol free herbal beverages. The sensory panel consisted of 40 students of master's and also doctoral study programme at Faculty of chemistry, University of Technology Brno. Four subsequent experiments are introduced, each of them consisting of a few sensory tests and including corresponding sensory forms attached. The sensory tests arrangement was based on currently valid Czech Technical Standards, using a ranking, descriptive, paired comparison, sensory profile testing and scaling methods. For the purpose of statistical evaluation, Friedman, Kruskal-Wallis, PCA and Faktor analysis were used. Level of abilities and sensory experience of panelists were found comparable to consumer testing. The results include sensory characteristics of all of the beverages tested. In general, the one-component beverages were preferred to the two-component beverages and the additive colouring with aronia concentrate wasn´t found any benefitial, from the sensory point of view. The consumer questionnaire proved all of the samples to be very promising, but based on the sensory results, we would consider the mint and the sage-mint beverages being the most potentialy applicable samples.
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Preparation and characterization of alcohol-free beers with different flavour
Benešová, Pavla ; Vávrová, Milada (referee) ; Márová, Ivana (advisor)
This diploma thesis deals with preparation and characterization of alcohol-free beers with different flavours. Five flavoured and four pure, commercially available beers were tested. Three alcohol-free beers were then selected and used for preparation of flavoured beers. Ingredients used for production of flavour were honey, raspberry, ginger and cranberry. Analysis was focused mainly on substances of phenolic nature. Flavoured beers were also tested in sensory analysis. Ingredients used for flavouring of beers and production of both alcoholic and alcohol-free beers are described in theoretical part of the thesis. Analysis of polyphenols, flavonoids, technological characteristics and anthocyanins was performed using spectrophotometric method. Phenolic substances were identified and quantified by HPLC/PDA. Ascorbic acid was analysed by HPLC-NH2/UV. Results proved an increase of polyphenols, ascorbic acid and anthocyanins during flavouring of alcohol-free beers in dependence of used ingredients. Sensory analysis showed that as the best rated was the Staropramen beer with raspberry flavour. On the contrary, flavoured Bernard beers were poorly rated.
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Complex processing of technical hemp and application of isolated fractions
Kohútová, Darina ; Němcová, Andrea (referee) ; Márová, Ivana (advisor)
Hemp is a plant containing many active substances and it is widely used in industry. The most common applications are the food, pharmaceutical, textile and cosmetics industries. This diploma thesis dealt with the characterization of hemp fractions, which are created during hemp processing and their potential applications. The theoretical part was focused on the description of the plant, its chemical composition and the research of the active substances in hemp. Also, its industrial use, the technique of hemp processing and the current applications of individual fractions were summarized. In the experimental part, hemp fractions were analysed in terms of nutrient content. Hemp extracts were prepared using three solvents - hexane, 70% ethanol and distilled water, which were compared based on the extraction yield of polyphenols, flavonoids, antioxidants, chlorophylls and cannabinoids. Antimicrobial effects were analysed in hexane and ethanol extracts. By-products of hemp processing were used in some screening application in biotechnology, cosmetics and nutrition. The results show that the extract with 70% ethanol had the highest content of active substances. From the hemp fractions, hemp trichomes and Fedora flowers had the highest content of active substances. Cannabis by-products have the potential to become valuable substrates for yeast cultivation. Face scrub containing hemp flour as an abrasive component had favourable results and was acceptable to consumers, as was the prepared hemp flour raw bar, which was evaluated to be comparable to commercially available hemp protein raw bars.
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Sensory evaluation of different types of meat products
Lanžhotská, Aneta ; Vespalcová, Milena (referee) ; Diviš, Pavel (advisor)
The diploma thesis deals with sensory evaluation of selected types of meat products, specifically sausages. Different types of sausages, which contained added chemicals and spices, were compared. The theoretical part describes the properties of meat and meat products and the basic technological procedures used for their processing and production. Furthermore, added substances and spices, which are often considered as flavorings or preservatives, were characterized. The principles of correct performance of sensory analysis and sensory perception of food were also presented, which include a description of the sensory workplace, sample preparation, its implementation, procedure and various evaluation methods used. The experimental part describes the specific work tools used and the conditions under which the evaluation took place. A total of 12 types of sausages were evaluated, 7 of which came from meat production and 5 were prepared in the laboratory of food chemistry at Brno University of Technology. The differences between the samples of sausages, which differed in content and type of added chemicals and spices, were clearly shown using ray graphs. Then the Grubbs test was used, which excluded outliers from the final evaluation. These values were further excluded from further evaluation. The Kruskall-Wallis test was used to distribute the resulting mean values and to determine whether there was a statistically significant difference in sensory evaluation between the results. An appendix to the Kruskall-Wallis test Dunn's test was used to divide the resulting values into given groups according to statistically significant difference and similarity. The analysis of the main components of the so-called PCA was used to find the differences and similarities of the samples included in the groups.
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Influence of aroma compounds on sensory quality of sweets
Motúzová, Eliška ; Zemanová, Jana (referee) ; Vítová, Eva (advisor)
This master´s thesis is focused on determination of aroma active compounds in non-chocolate sweets and their influence on sensory quality/flavour. The theoretical part describes production of non-chocolate sweets, characterizes aroma active compounds including allergenic ones, method for their determination by solid-phase microextraction and gas chromatography with mass detection. For experimental part were used as model samples gummy candies purchrased in regular market. Four flavors were used, specifically yellow (lemon), green (apple), red (strawberry) and orange (orange). The HS-SPME-GC-MS method was used to identify volatile aroma active compounds. To evalutate the sensory quality were used scales, a profile test, and a time course evaluation. Owerall 120 volatille compounds were identified including 8 allergens.
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Production and characterization of non-chocolate sweets with the addition of plant extracts
Knapovská, Pavlína ; Nábělek, Jakub (referee) ; Vítová, Eva (advisor)
This bachelor thesis deals with the production and characterization of plant extracts of green tea (Camellia sinensis) and sea buckthorn (Hippophae rhamnoides) and non-chocolate sweets – hard candies with the addition of plant extracts. These plants are characterized by a high content of bioactive substances, upon which the prepared extracts and candies were characterised. Determination of volatile substances by HSSPME-GC-MS, antioxidant activity by method with ABTS, determination of total phenols by Folin-Ciocalteu method and sensory analysis were performed. The produced sweets were then compared with similar commercial products. A total of 6 volatile substances were identified in the sample of hard candy with the addition of green tea extract, the antioxidant activity of the sample was determined to be 253,3 ± 0,9 µgml–1 and the total phenolic content was determined to be 0,49 ± 0,02 mgGAEg–1 per 1 g of candy. A total of 8 volatile substances were identified in the hard candy with the addition of sea buckthorn extract, the antioxidant activity of the sample was determined to be 206 ± 3 µgml–1, the total phenolic content was determined to be 0,51 ± 0,04 mgGAEg–1 per 1 g of candy. The obtained results show that the addition of extracts increases the nutritional value (higher antioxidant activity, higher content of phenolic compounds) of produced candy; from a sensory point of view, however, only samples with green tea were evaluated positively, the addition of sea buckthorn extract was not very attractive for consumers, they considered sweets with sea buckthorn to be too bitter.
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Sensory quality of chosen types of meat products
Javorská, Kateřina ; Sůkalová, Kateřina (referee) ; Vítová, Eva (advisor)
This Bachelor’s thesis deals with the evaluation of sensory quality of meat products. The 5 samples of pork ham of different quality and from different producers were selected for evaluation. The theoretical part focuses on the composition and properties of meat and meat products, and their production technology. The selected methods for sensory evaluation of quality are also mentioned. Ordinal and graphical scales were used for sensory evaluation of samples. The assessors evaluated these basic characteristics of the ham samples - color, appearance in the cut, preparation, consistency, smell, taste, saltiness and tenderness. The results were written down in the prepared questionnaires which were subsequently statistically and graphically evaluated. Sensory analysis was performed by group of students from the Faculty of Chemistry BUT.
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The effect of the quantity and type of nuts used in the production of pâté on the product sensory quality
Mejzlíková, Blanka ; Kovalčík, Adriána (referee) ; Mikulíková, Renata (advisor)
This bachelor thesis deals with the sensory evaluation of pâté with nuts. The 9 samples of pâtés which contained different quantity of nuts and different types of nuts were produced for sensory evaluation. In the theoretical part pâtés are characterised, their history, composition and manufacturing are described. The part of literature search is description and chemical composition of nuts which were used for production of pâtés. Another topic is a description of conditionals and methods of sensory analysis and sensory evaluation of meat products. The practical part comprises production of test samples, samples for sensory analysis, evaluating the heat treatment and sensory analysis. Percentage amount of lipids and composition of fatty acids were determined by gas chromatography. Processing of results of sensory and chemical analysis of samples of pâtés was determined that the best samples were pâtés with cashew nuts and pistachios.
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Alcohol-free beers with herbal extracts
Vyskočilová, Terezie ; Lichnová, Andrea (referee) ; Márová, Ivana (advisor)
This bachelor thesis deals with alcohol-free beers and herbal extracts, which are the beers flavoured with. In the theoretical part the methods of non-alcoholic beer production and the representation of the active ingredients in herbs are described. A total of 7 herbal extracts were prepared and added into 3 non-alcoholic Czech beers. Each variant was examined in order to detect changes of the content of active substances before and after the addition of the herbal extract. The content of polyphenols, flavonoids, bitter substances and iso-bitter acids as well as antioxidant activity were analyzed spectrophotometrically. Using HPLC/UV-VIS analysis a content of catechins was assayed. The two selected flavours were encapsulated into liposome; the particles were tested of size and stability. Sensory analysis of all herbal extracts including the encapsulated ones was performed to determine the response of consumers and flavour preferences.
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