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Characterization of bacterial strains obtained in evolutionary engineering
Hrabalová, Vendula ; Sedláček, Petr (referee) ; Obruča, Stanislav (advisor)
This diploma thesis deals with application of evolutionary engineering on PHA producing bacterial strains. Two bacterial strains, Cupriavidus necator H16 and Halomonas halophila, were chosen for the evolutionary experiments. Copper cations (Cu2+) and sodium chloride (NaCl) were chosen as the selective pressure for C. necator H16; acetic acid (AA) and levulinic acid (LA) for Halomonas halophila. The adapted strains were during long-time evolutionary experiments characterized by GC-FID and SEC-MALS. The growth of the adapted strains was studied by the mean of optical density measurement. The amount of viable cells was determined by spectral FC after their expositon to selected stress factors. Specific enzyme activities of enzymes involved in citrate and glyoxalate cycle, enzymes generating NADPH, LA metabolism enzyme and PHA biosynthesis enzymes were determined. The adapted strains were compared with the wild-type of strains. The successfull adaptation of C. necator H16 adapted to Cu2+ was detected. Biomass and PHA production of both wild and adapted H. halophila strains cultivated in lignocellulosis waste were determined. It was found out that H. halophila adapted to the LA is capable of producing more PHA than the wild strain of this bacteria.
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Adaptible Joystick Interpret for RDS
Erben, Vojtěch ; Burian, František (referee) ; Honzík, Petr (advisor)
The main subjects of this bachelor thesis are proposal and implementation of control adaptation on concrete user. Adaptation is achieved by proposed adaptable interpreter, which changes joystick behavior using conversion function. Parameters of the conversion function are set by evolution algorithm based on recent drive through given path. The development environment Microsoft Robotics Developer Studio (MRDS) is described in the introduction including instructions for creating a simple project. Furthermore, there is analyzed way of work with joystick and adjustments to his behavior. Follows description of way of programming in MRDS, concerning getting robot object from simulation and its automatic driving. Next part of this work describes proposal and implementation of adaptable joystick interpreter. There is proposed equation of the conversion function used in this interpreter and described the way of setting its parameters. Last part describes methods of testing and test results.
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Employee Adaptation in the Selected Company
Romanová, Eva ; Čuta, Jaromír (referee) ; Konečný, Štěpán (advisor)
This master’s thesis deals with the adaptation process of employees in the selected company. The theoretical part is focused on explaining the issues of basic concepts related to this topic. I analyse adaptation process of Company XY a.s. in practical part and then I present proposals in this area that could help improve the company’s adaptation process.
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Unsupervised Adaptation of Speech Recognizer
Švec, Ján ; Karafiát, Martin (referee) ; Schwarz, Petr (advisor)
The goal of this thesis is to design and test techniques for unsupervised adaptation of speech recognizers on some audio data without any textual transcripts. A training set is prepared at first, and a baseline speech recognition system is trained. This sistem is used to transcribe some unseen data. We will experiment with an adaptation data selection process based on some speech transcript quality measurement. The system is re-trained on this new set than, and the accuracy is evaluated. Then we experiment with the amount of adaptation data.
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Revitalization of the castle Uherčice
Křídová, Nikola ; Šindlar, Jiljí (referee) ; Hrabec, Josef (advisor)
This diploma project deals with architectural study and functional use of the Uherčice castle grounds with regard to its character, location in the countryside and in the village, preservation of monuments and requirements of the inserted functions, as well as the creation of new opportunities for visitors and the reconnection to life of the inhabitants of the Uherčice village. And as a place of rest, education, employment and as a re-center of the area. A proposal of modification of the selected part of the castle and the park with the restoration of the French garden in its original place is a part of the project. The reconstruction of the former orangery and the new greenhouse building are proposed. Re-build greenhouse is attached with an apartment for the gardener. The second greenhouse complements one of the castle's axes and is designed for the world's largest water lily, Victoria Amazonica. In the selected part of the chateau, I mainly dealt with its use for cultural and social events that could take place here and the facilities that would be needed, including an apartment for short-term accommodation of visitors to these events. In addition, the entrance area and the operational facilities of the castle with a castellan apartment and accommodation for tourist guides are also solved.
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Strategy for Improvement of Training System of Company
Gazurová, Anna ; Kufa, Jan (referee) ; Mallya, Thaddeus (advisor)
This thesis focuses on problems related with new graduates at the „Department of New Employees Training“ of TŘINECKÉ ŽELEZÁRNY joint stock Company. The work evaluates to what extent the participants are satisfied with the training period, work of each mentor assigned to them and how they are involved in solving concreate tasks. Later suggestions are made on what measures should be taken to improve the program, for future participants to be satisfied.
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Speaker Recognition in the VoIP Environment
Remeš, Jan ; Pešán, Jan (referee) ; Plchot, Oldřich (advisor)
Tato práce popisuje použití systémů pro rozpoznávání mluvčího v~prostředí VoIP, úspěšnost systému a přístupy k jejímu zlepšení. Popisuje architekturu těchto systémů, metriky pro vyhodnocení jejich úspěšnosti a klíčové komponenty VoIP z hlediska rozpoznávání mluvčího. Je zde popsáno vytvoření simulace VoIP prostředí, úspěšnost systému je vyhodnocena na datech pocházejících z různých druhů VoIP prostředí a výsledky jsou demostrovány. Adaptace a kalibrace systému je provedena a jejich přínosy zhodnoceny.
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Evolutionary engineering of polyhydroxyalkanoates producing bacteria
Nováčková, Ivana ; Kovalčík, Adriána (referee) ; Obruča, Stanislav (advisor)
This diploma thesis deals with the application of evolutionary engineering to PHA producing bacterial strains. The aim of the thesis is to prepare strains adapted to levulinic acid, a selected stress factor, by methods of evolutionary engineering, and then to characterize these strains. The theoretical part deals with evolutionary engineering and polyhydroxyalkanoates predominantly. The bacterial strain Cupriavidus necator H16 was used for evolutionary experiments. Levulinic acid and levulinic acid in the presence of the MMS mutagen were applied to prepare adapted strains. Selection of mutants was evaluated on the basis of growth potential and PHA content in biomass. Polymers produced by five obtained PHA-producing mutants and control were characterized using GC-FID, SEC-MALS, DSC and FT-IR. It was found that a higher content of 3HV in the copolymer led to a lower crystallinity and hence to a lower melting point, nevertheless, only the copolymer of the M0151 strain did not fit this trend. In addition to the characteristics of the polymers, the strains themselves were evaluated from the biochemical point of view by determining the activities of selected enzymes of the citrate, glyoxalate and 2-methylcitrate cycle, selected enzymes generating NADPH, levulic acid catabolism enzyme and PHA biosynthesis enzymes. On the basis of the obtained data, the possible adaptation strategies were discussed, when the E0575 strain was most differentiated from original culture. Values of specific enzyme activities were subjected to AHC and PCA statistical analysis methods.
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