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Vliv délky a způsobu transportu jiker na jejich líhnivost u hlavatky obecné (Hucho hucho)
BERÁNEK, Lukáš
The success of transporting both unfertilized and fertilized artificially stripped ova of huchen from the farm for up to 5 days was tested. A mixture of ova from 8 females was divided into two parts. The first part was inseminated with artificially stripped sperm (a mixture from several males) on-site (1 hour after stripping), and after rinsing the fertilized ova with water, it was placed in water in a plastic sealable container. The second part of the ova, along with the ovarian fluid, was not inseminated with sperm and was placed in another identical container. Both containers were stored in a thermal insulation box for the following 5 days, including transportation from the site of artificial stripping to the incubation site. The mixture of collected sperm from 5 males was stored in syringes in a separate isothermal box at a temperature of +4°C. Both thermal insulation boxes were placed in the luggage compartment of a passenger car for 5 days. For the ova transported in water, the water was exchanged twice a day. Throughout the first day, both the ova and sperm were transported from the site of artificial stripping to a distance of 350 km to the incubation site. In the following days, the car was driven three times a day for at least 0.5 hour each time to simulate vibrations occurring during transportation. The ambient temperature for the ova during transportation was maintained at 10°C. Samples of both fertilized and unfertilized ova were taken at one-day intervals from the artificial stripping, and after counting and fertilization of the ova with transported sperm, they were rinsed and placed in small incubation dishes with separate water inflows and perforations allowing water flow and preventing the escape of subsequently hatched embryos. Thirty ova were always placed in each variant, with 3 repetitions in all variants. The incubation dishes were placed on the bottom of a shallow flow-through channel supplied with water from a recirculating system with cold-water fish farming. The average water temperature during incubation was 12.8°C. Mortality of the ova was monitored during incubation, white unfertilized or dead ova were removed, and hatching of the embryos was recorded. In the first variant, after stripping the fertilized ova transported in water for one, two, or three days, a hatchability of 32.2 ? 21.1%, 27.8 ? 10.3%, and 33.3 ? 32.1%, respectively, was achieved. After simulated transportation for four and five days, the hatchability decreased to 21.1 ? 1.57% and 15.6 ? 8.3%, respectively. The differences between all the aforementioned parameters are not statistically significant. In the second variant, with unfertilized ova transported in ovarian fluid, a comparable hatchability (34.4 ? 11%) was achieved after one day of transportation, as with fertilized ova transported in water for one to three days. Unfertilized ova transported in ovarian fluid for a longer period (two and three days) showed a statistically significant decrease in hatchability to 7.8 ? 8.8% and 2.2 ? 1.6%, respectively. A 100% mortality rate was found for ova transported in unfertilized ovarian fluid for four and five days. No differences were found in the length of the incubation period due to different methods or lengths of ova transportation. The experiment demonstrated the feasibility of using transportation of fertilized ova in water for several days, with transportation of fertilized ova in the aquatic environment being preferable.
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Vliv teploty na schopnost oplození a líhnivosti při krátkodobém skladování neoplozených jiker síha peledě (Coregonus peled)
RYTÍŘ, Jan
The northern whitefish Coregonus peled (Gmelin, 1788), originally from Russia, was introduced to the Czech Republic in 1970 for its tasty meat and good growth ability. Breeding of this species has been very popular in the past. Currently, thanks to the fish-eating predators, traditional breeding of this species is on decline. The aim of this M. Sc. Thesis was to sumarize the avaible information in the field of peled biology, artificial propagation and also information about effect of temperature on the ability of fertilization and hatching for short-term of unfertilized eggs in other salmonid species. In the practical part, the influence of temperature and length of storage of stripped, unfertilized eggs of peled on fertilization, survival to eyed eggs and hatching were observed. Unfertilized eggs were divided into five bowls and deposited in thermo boxes, which were tempered to 2.5, 5, 7.5, 10 and 12.5 °C. After time intervals of 1, 4, 12, 24, 36, 48, 60 and 72 hours, approximately 100 - 200 eggs were taken from each thermobox, which were fertilized with fresh sperm (collected from several males) and water from the hatchery. The fertilized and purified eggs were moved to the incubators with continuously inflow of fresh water. Dead eggs were removed and recorded. Subsequently, fertilization, eyed eggs and hatching eggs were determined. The resulting values of these parameters were expressed as a percentage of the total number of used eggs. High levels of fertilization and survival to eyed eggs were achieved when stored within 1 day from eggs stripping at all temperatures except the highest temperature of 12.5 ° C. As the interval gradually lengthened, the fertilization and survival parameters also decreased, most notably at 7.5 and 10 ° C. To obtain the largest possible amount of fry in fishing practice, it is recommended to store eggs at 2.5 and 5 ° C, up to 48 hours after ova stripping. At higher temperatures, the effective storage time is reduced to 12 hours. Storing eggs for longer than 48 hours, in practical terms has no meaning. At the same time, it was found that at the above-mentioned optimal temperatures for storing eggs (2.5 and 5 ° C), the best hatching results were obtained when storing stripped eggs for 12 hours. Not only for longer, but also for shorter storage lengths, the values of this parameter were lower.
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The awareness of high school students about sexually transmitted diseases
Roubalová, Michaela ; Matulová, Jana (advisor) ; Vachková, Eva (referee)
64 ANNOTATION Author: Michaela Roubalová Institution: Institute of Social Medicine, Charles University, Faculty of Medicine in Hradec Kralove, Department of Nursing The name of the work: The awareness of high school students about sexually transmitted diseases Supervisor: Mgr. Jana Matulová The number of pages: 76 The number of attachments: 3 Defended in: 2022 Key words: sexually transmitted diseases, awareness of high school students, vaccination, prevention This bachelor thesis deals with the awareness of high school students about sexually transmitted diseases. It is divided into theoretical and empirical parts. In the theoretical part is contained the history, diagnosis, prevention against sexually transmitted diseases and examples of sexually transmitted diseases. The thesis also deals with male anticonception, adolescence, and sex education. In the empirical part, we focus on the range of knowledge of high school students about sexually transmitted diseases.
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Progress in Edition of Small Magazines
Podzimek, Jan ; Syřiště, Ivo (advisor) ; Mojžíšová, Jarmila (referee)
TITLE: Progress in Edition of Small Magazines AUTHOR: Bc. Jan Podzimek DEPARTMENT: Department of Education SUPERVISOR: Doc. PhDr. Ivo Sy išt , Ph.D. ABSTRACT: Small magazines published by a volunteers are a phenomenon. They rise and fall without much control and regulation. This thesis continues on the bachelor thesis "Small Magazines" and maps the design and development of various small magazines after five and twenty years. In this thesis are little magazines put into the context of the Scout educational method. On results of competition "sCOOL web 2015" is estimated percentage representation of school magazines in schools. KEYWORDS: small magazine, school magazine, group magazine, media education, progress
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Bordetella Adenylate Cyclase: Molecular Mechanism of Action and Its Use for Antigen Delivery
Kamanová, Jana
Dissertation title: Bordetella Adenylate Cyclase: Molecular Mechanism of Action and Its Use for Antigen Delivery Author: Jana Kamanová The first part of this PhD. thesis deals with molecular mechanism of action of the adenylate cyclase toxin (CyaA), a key virulence factor of the whooping cough agent Bordetella pertussis. CyaA belongs to the family of RTX (Repeat-in-ToXin) proteins secreted by Gram-negative bacteria and primarily targets myeloid phagocytes, expressing the CD11b/CD18 integrin receptor (also known as αMβ2, CR3 or Mac-1). Upon binding, CyaA permeabilizes cell membranes by forming small cation-selective pores, and subverts cellular signaling by delivering into host cells an adenylate cyclase (AC) enzyme that converts ATP to cAMP. Elevation of the cytosolic cAMP levels by CyaA then knocks down bactericidal functions of host innate immunity. CyaA is unique among other enzymatically active toxins in its capacity to penetrate cells directly from cell surface across the cytoplasmic membrane, without the need for endocytosis. Penetrating activity of CyaA depends on plasma membrane potential and on an intact, acylated and calcium-loaded RTX cytolysin moiety. By examining a set of 18 CyaA constructs that bear overlapping deletions within AC domain and a CD8+ OVA T-cell epitope tag, we showed that the...
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Bordetella Adenylate Cyclase: Molecular mechanism of Action and Its Use for Antigen Delivery
Kamanová, Jana ; Šebo, Peter (advisor) ; Dráber, Petr (referee) ; Černý, Jan (referee)
(English) 4 SUMMARY (English) The first part of this PhD. thesis deals with molecular mechanism of action of the adenylate cyclase toxin (CyaA), a key virulence factor of the whooping cough agent Bordetella pertussis. CyaA belongs to the family of RTX (Repeat-in-ToXin) proteins secreted by Gram-negative bacteria and primarily targets myeloid phagocytes, expressing the CD11b/CD18 integrin receptor (also known as αMβ2, CR3 or Mac-1). Upon binding, CyaA permeabilizes cell membranes by forming small cation-selective pores, and subverts cellular signaling by delivering into host cells an adenylate cyclase (AC) enzyme that converts ATP to cAMP. Elevation of the cytosolic cAMP levels by CyaA then knocks down bactericidal functions of host innate immunity. CyaA is unique among other enzymatically active toxins in its capacity to penetrate cells directly from cell surface across the cytoplasmic membrane, without the need for endocytosis. Penetrating activity of CyaA depends on plasma membrane potential and on an intact, acylated and calcium-loaded RTX cytolysin moiety. By examining a set of 18 CyaA constructs that bear overlapping deletions within AC domain and a CD8+ OVA T-cell epitope tag, we showed that the first 371 amino-terminal residues are dispensable for the CyaA capacity to deliver a passenger OVA...
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Gender analysis of the documents intended to eliminate social exclusion
Popelková, Veronika ; Sokolová, Věra (advisor) ; Jiroutová Kynčlová, Tereza (referee)
Submitted thesis is addressing the phenomenon of social exclusion from the gender perspective. Local issues of social exclusion is trying to resolve Central Bohemian town Kralupy nad Vltavou by producing written documents as an instrument to deal with this phenomenon. Documents look into the current situation and possibilities for change in order to achieve gradual elimination of negative effects related to social exclusion in all domains of citizens live, male or female, should they be affected by social exclusion. By using the methodology of critical gender qualitative content analysis Im to find out the way the concerned documents include the subject of gender and what role the gender stereotypes play. In the theoretical section I focus on the field of social work, which is as a discipline, heavily affected by stereotyped gender views and since its origins, faces constant misconceptions of being predominantly female profession. Key words: social exclusion, gender intersection, homelessness, institution, social integration, ethnicity, threat, gender, family, female, male
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Progress in Edition of Small Magazines
Podzimek, Jan ; Syřiště, Ivo (advisor) ; Mojžíšová, Jarmila (referee)
TITLE: Progress in Edition of Small Magazines AUTHOR: Bc. Jan Podzimek DEPARTMENT: Department of Education SUPERVISOR: Doc. PhDr. Ivo Sy išt , Ph.D. ABSTRACT: Small magazines published by a volunteers are a phenomenon. They rise and fall without much control and regulation. This thesis continues on the bachelor thesis "Small Magazines" and maps the design and development of various small magazines after five and twenty years. In this thesis are little magazines put into the context of the Scout educational method. On results of competition "sCOOL web 2015" is estimated percentage representation of school magazines in schools. KEYWORDS: small magazine, school magazine, group magazine, media education, progress
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Bordetella Adenylate Cyclase: Molecular Mechanism of Action and Its Use for Antigen Delivery
Kamanová, Jana
Dissertation title: Bordetella Adenylate Cyclase: Molecular Mechanism of Action and Its Use for Antigen Delivery Author: Jana Kamanová The first part of this PhD. thesis deals with molecular mechanism of action of the adenylate cyclase toxin (CyaA), a key virulence factor of the whooping cough agent Bordetella pertussis. CyaA belongs to the family of RTX (Repeat-in-ToXin) proteins secreted by Gram-negative bacteria and primarily targets myeloid phagocytes, expressing the CD11b/CD18 integrin receptor (also known as αMβ2, CR3 or Mac-1). Upon binding, CyaA permeabilizes cell membranes by forming small cation-selective pores, and subverts cellular signaling by delivering into host cells an adenylate cyclase (AC) enzyme that converts ATP to cAMP. Elevation of the cytosolic cAMP levels by CyaA then knocks down bactericidal functions of host innate immunity. CyaA is unique among other enzymatically active toxins in its capacity to penetrate cells directly from cell surface across the cytoplasmic membrane, without the need for endocytosis. Penetrating activity of CyaA depends on plasma membrane potential and on an intact, acylated and calcium-loaded RTX cytolysin moiety. By examining a set of 18 CyaA constructs that bear overlapping deletions within AC domain and a CD8+ OVA T-cell epitope tag, we showed that the...
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