National Repository of Grey Literature 223 records found  1 - 10nextend  jump to record: Search took 0.01 seconds. 
Wnt/beta-catenin and mTOR signaling in regulation of T-cell phenotype and cytotoxic activity for adoptive cellular immunotherapy of cancer
Stakheev, Dmitry ; Smrž, Daniel (advisor) ; Černý, Jan (referee) ; Říhová, Blanka (referee)
1. Abstract (EN) The adoptive cellular immunotherapy (ACI) based on ex vivo produced T cells is a modern treatment modality of cancer. However, the ex vivo production of T cells with high therapeutic efficacy is far to be well established. Wnt/β-catenin and mTOR signaling have been shown to affect both cancer cells and immune cells. Therefore, the modulation of these pathways seems to be perspective for the production of T cells with superior therapeutic efficacy. The aim of our project was to investigate, how interventions into Wnt/β-catenin and mTOR signaling during the ex vivo production of tumor-associated antigen-specific T cells could improve the production of T cells with a desired and controlled phenotype that would best fit for use in ACI of cancer. In the first part of our study, we investigated the role of Wnt/β-catenin inhibition by XAV939 on cancer cell elimination by lymphocytes from patients with localized biochemically recurrent prostate cancer (BRPCa). We found that preconditioning BRPCa lymphocytes with 5 µM XAV939 accelerated the elimination of LNCaP and PC3 cells during the coculturing. However, during subsequent re-coculturing with fresh LNCaP cells, BRPCa lymphocytes were no longer able to eliminate cancer cells unless coculturing and re-coculturing were performed in the presence of...
The Secularization of the Prague Working Class, Smichow and Its Vicinity in the Second Half of the 19th Century.
Černý, Jan Karel ; Štaif, Jiří (advisor) ; Pavlíček, Tomáš (referee)
(in English) A thesis deals with the secularization of a Prague, especially Smichow working class in the second half of the 19th century. The working class is usually considered the most secular stratum and the aim of the thesis is to verify the proposition locally and to examine why the process of secularization occurred in the working class so powerfully. The working class is drafted as a social stratum in which the process acts as well as the stratum actively practices it. The process of secularization is then divided into three categories; religion - spirituality - church and the process is conceptualized as "de-catholization" as well as the spiritual transformation. In the process of the transformation, traditional religion (Catholicism), its institutions (church) and religiosity lose their socio-cultural power and are replaced by modern ideologies, state (bureaucratic and rational) structures and implicit spirituality, resp., "un- religious faith". In the process of secularization, there is particularly stressed the role of urbanization, industrialization, socialism, church and its social teaching. In the thesis, there are combined theoretical approaches of the sociology of religion (the sacred canopy) and religious studies (dimensions of the Sacred) and are applied to the secondary and...
The Secularization of the Prague Working Class, Smichow and Its Vicinity in the Second Half of the 19th Century.
Černý, Jan Karel ; Štaif, Jiří (advisor) ; Pavlíček, Tomáš (referee)
(in English) A thesis deals with the secularization of a Prague, especially Smichow working class in the second half of the 19th century. The working class is usually considered the most secular stratum and the aim of the thesis is to verify the proposition locally and to examine why the process of secularization occurred in the working class so powerfully. The working class is drafted as a social stratum in which the process acts as well as the stratum actively practices it. The process of secularization is then divided into three categories; religion - spirituality - church and the process is conceptualized as "de-catholization" as well as the spiritual transformation. In the process of the transformation, traditional religion (Catholicism), its institutions (church) and religiosity lose their socio-cultural power and are replaced by modern ideologies, state (bureaucratic and rational) structures and implicit spirituality, resp., "un- religious faith". In the process of secularization, there is particularly stressed the role of urbanization, industrialization, socialism, church and its social teaching. In the thesis, there are combined theoretical approaches of the sociology of religion (the sacred canopy) and religious studies (dimensions of the Sacred) and are applied to the secondary and...
Maternal allergy status has no impact on neonatal immune responses to allergen stimuli
Lohonková, Adéla ; Hrdý, Jiří (advisor) ; Černý, Jan (referee)
The alarming increasing incidence of allergic diseases leads to the effort to identify the group of markers with prognostic potential pointing reliably to an allergy development at a later age. Identification of such marker or a group of markers pointing to a higher risk of developing an allergy would allow early implementation of preventive measures to block further development of the allergic disease. Umbilical cord blood appears to be the ideal biological material for the search for prognostic markers that are suitable for further study. The ability of umbilical cord blood cells to respond to stimulation by common allergens by producing cytokines according to a pattern dependent on the allergic status of the mother could be the predictive feature sought. The presented study focuses on the determination of cytokines typical for Th1- : interferon gamma (IFN-γ), Th2-: interleukin 5, interleukin 13 (IL-5, IL-13) a regulatory T cells (Tregs): interleukin 10 (IL-10) immunity responses at the level of gene expression by quantitative real-time polymerase chain reaction (qRT-PCR) followed by determination of the protein production itself in the cell culture supernatant. Newborns were divided into two groups according to the allergic status of their mothers: children of healthy mothers (newborns with a...
Establishment and characterization of mouse tumor cell line with irreversible downregulation of MHC class I molecules
Lhotáková, Karolína ; Poláková, Ingrid (advisor) ; Černý, Jan (referee)
Malignant diseases are one of the major causes of death worldwide and the most common reason is the ability of tumor cells to escape the immune system. One of the main mechanisms used by tumors to avoid the immune response is downregulation of MHCI expression. In this thesis, the preparation of tumor cell line with irreversible downregulation of B2m is described. Inactivation of B2m was confirmed by flow cytometry. However, MHCI heavy chain H2-Db were expressed at the cell surface in a small amount after stimulation with IFNγ. In vitro proliferation of this clone was decreased. The tumor formation was delayed, and this effect was mediated by NK cells that played the major role in antitumor immunity. Metastatic activity of these cells was not affected after inactivation of B2m. TC-1/dB2m cells are not sensitive to DNA immunization. In combined immunotherapy experiments with DNA vaccinaton and ODN1826 adjuvant, a weak deceleration of tumor growth was achieved repeatedly. TC-1/dB2m induced tumors contained more CD4+ T lymphocytes, Treg, γδ T lymfocytes and plasmacytoid dendritic cells, and fewer NK cells and macrophages than tumors induced by original TC-1 cells. Moreover, combined immunotherapy did not increase the infiltration of the TC-1/dB2m tumors with immune cells. The TC-1/dB2m tumor cell line...
The role of ORMDL3 in the IgE receptor signaling
Vávrová, Petra ; Bugajev, Viktor (advisor) ; Černý, Jan (referee)
ORMDL proteins are regulators of serine palmitoyltransferase (SPT) enzyme, which catalyzes the first step of sphingolipid biosynthesis. Human and murine ORMDL family consists of three members, ORMDL1, ORMDL2 and ORMDL3. Human and murine ORMDLs exhibit high similarity between their amino acid sequences. ORMDL3 expression has been linked to several diseases, such as childhood-onset asthma, Crohn's disease, rheumatoid arthritis, type 1 diabetes, and primary biliary cirrhosis. High expression of ORMDL3 has been found in macrophages, T cells, eosinophils, epithelial cells and mast cells. ORMDL3 is a negative regulator of the high affinity IgE receptor I (FcɛRI)-mediated signaling in mast cells. Mast cells have an important role in the acute phase of allergic reactions and are involved in eradication of multicellular parasites. In the first part of this thesis we determined the expression of ORMDL family in peritoneal- derived mast cells (PDMCs) from Ormdl3 knock out (KO) and wild type (WT) mice. Next we determined the roles of ORMD3 in FcɛRI-mediated signaling in these PDMCs. Furthermore, we analyzed the relationship between expression of ORMDL family and SPT complex in bone marrow-derived mast cells (BMMCs) and bone marrow-derived mast cell line (BMMCL). We transduced BMMCL with vector coding SPTLC1...
Wnt/beta-catenin and mTOR signaling in regulation of T-cell phenotype and cytotoxic activity for adoptive cellular immunotherapy of cancer.
Stakheev, Dmitry ; Smrž, Daniel (advisor) ; Černý, Jan (referee) ; Říhová, Blanka (referee)
1. Abstract (EN) The adoptive cellular immunotherapy (ACI) based on ex vivo produced T cells is a modern treatment modality of cancer. However, the ex vivo production of T cells with high therapeutic efficacy is far to be well established. Wnt/β-catenin and mTOR signaling have been shown to affect both cancer cells and immune cells. Therefore, the modulation of these pathways seems to be perspective for the production of T cells with superior therapeutic efficacy. The aim of our project was to investigate, how interventions into Wnt/β-catenin and mTOR signaling during the ex vivo production of tumor-associated antigen-specific T cells could improve the production of T cells with a desired and controlled phenotype that would best fit for use in ACI of cancer. In the first part of our study, we investigated the role of Wnt/β-catenin inhibition by XAV939 on cancer cell elimination by lymphocytes from patients with localized biochemically recurrent prostate cancer (BRPCa). We found that preconditioning BRPCa lymphocytes with 5 µM XAV939 accelerated the elimination of LNCaP and PC3 cells during the coculturing. However, during subsequent re-coculturing with fresh LNCaP cells, BRPCa lymphocytes were no longer able to eliminate cancer cells unless coculturing and re-coculturing were performed in the presence of...
Noncanonical functions of IL-1α
Novák, Josef ; Pospíšek, Martin (advisor) ; Černý, Jan (referee) ; Brdička, Tomáš (referee)
1α (IL 1α) is a multifunctional cytokine 1α is 1α independent on the receptor sig 1α is responsible for 1α to the plasma membrane. 1α activates express κB, binds to 1α 1α 1α to the plasma membrane 1α to signal 1α is required for membrane 1α exter 1α anchoring 1α 1α 1α with tumor suppressor p53 following genotoxic stress is further described in human cell 1α coloca
AIRE-expressing cells in immune tolerance in health and disease
Vobořil, Matouš ; Filipp, Dominik (advisor) ; Černý, Jan (referee) ; Ehrlich, Lauren Ilyse Richie (referee)
The process of self-nonself discrimination by the immune system is a fundamental attribute of healthy organisms. Since T-cell receptors (TCRs) are generated by the random process of somatic recombination without regard to its targets, the newly developed T-cell clones could recognize either self or nonself antigens. The mechanisms of central tolerance robustly limit the self-reactive repertoire within the T-cell population via deletion of clones that express self-reactive TCRs or their deviation into the regulatory T-cells (Tregs). These processes occur mainly in the thymic medulla where the TCR reactivity to self-antigens is tested by various types of antigen-presenting cells, mainly medullary thymic epithelial cells (mTECs), dendritic cells (DCs), and B-cells. The cooperation between these cell-types has been shown to be essential for the establishment of thymic tolerance. A key molecule regulating the production of self-antigens is the autoimmune regulator (AIRE), which is thought to be expressed primarily by mTECs and its mutations are associated with the development of severe autoimmune disorders. In this context, the presented thesis describes the novel regulatory pathways important for the development of a functional and "harmless" repertoire of T-cells and for enforcement of tolerance....
Continual double-helicoidal mixer
Černý, Jan ; Malach, František (referee) ; Malášek, Jiří (advisor)
This work focuses on the process of mixing particulate materials and the design of the screw mixer for this activity. The theoretical part describes the mixing and properties of particulate matter. Subsequently, individual devices serving for different types of mixing are analyzed. The practical part contains calculations of the main dimensions and the required power consumption. The choice of suitable drive, bearings and gear is followed. Finally, the analysis of the blade shaft load and the strength analysis of the mixer blade using Autodesk Inventor are performed. Part of this work is enclosed drawing documentation of equipment assembly and selected partial components.

National Repository of Grey Literature : 223 records found   1 - 10nextend  jump to record:
See also: similar author names
54 ČERNÝ, Jan
4 ČERNÝ, Jaroslav
27 ČERNÝ, Jiří
3 ČERNÝ, Josef
36 Černý, Jakub
1 Černý, Jan Bc.
3 Černý, Jan Karel
2 Černý, Jan,
1 Černý, Jaromír
4 Černý, Jaroslav
3 Černý, Jindřich
27 Černý, Jiří
3 Černý, Josef
2 Černý, Jáchym
54 Černý, Ján
Interested in being notified about new results for this query?
Subscribe to the RSS feed.