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Comparative study: Economies of Obazine and Plasy Monasteries in the Middle -ages (12.-13. century)
Müllerová, Gabriela ; Charvátová, Kateřina (advisor) ; Těšínská Lomičková, Radka (referee)
The thesis focuses on the economy of the Cistercian Order, from its inception until the end of the 13th century. There are two parallel analysis of monasteries Obazine (France) and Plasy (Bohemia), which are compared in the final part of the research. First the study present foundation of monasteries in the historical context of the country and the region. Then he focuses on the process of acquiring property and attempts to reveal common and different features in the economic development of the courts and farming. The work may present the real situation only to the extent that it allows the historical sources. The main historical source is cartulary of Obazine and documents of Plasy from Codex diplomaticus et epistolaris regni Bohemiae. In addition, the study draws information from various foreign and czech literature.
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The function of Pmp3p protein
Belovičová, Martina ; Palková, Zdena (advisor) ; Pichová, Iva (referee)
During development of Saccharomyces cerevisiae colonies on solid substrate, metabolic transitions occur, which are reflected by pH changes around the colony and also by changes in cell membrane potential. Analyses of transmemebrane potential oscillations revealed a function for Plasma Membrane Proteolipid 3 (Pmp3p) in this process. The thesis discusses possible function of Pmp3p protein mainly on the basis of direct observation of Pmp3p localization in vivo under variety of cultivation conditions. Yeast strains with different variants of Pmp3p protein fused with different protein tags that allow monitoring of Pmp3p localization and concentration in cells were prepared by homologous recombination. Localization of Pmp3p in the plasma membrane and in lipid particles was found. The Pmp3p level in cells was stable during development of colonies growing on either respirative or fermentative carbon source medium. High concentration of extracellular sodium chloride did not evoke increase in Pmp3p-GFP concentration. Key words: Pmp3p, proteolipids, UPF0057 (PMP3) family, Saccharomyces cerevisiae, long term survival, lipid particles
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Pre-clinical model of acute promyelocytic leukemia:/study of the anti-leukemic effect induced by ATRA and DNA vaccination
Pokorná, Kateřina ; Holáň, Vladimír (advisor) ; Stöckbauer, Petr (referee) ; Degos, Laurent (referee)
DOCTORAL THESIS 2012 POKORNA Abstract We have used a well characterized transplantable transgenic mouse model which mimics human acute promyelocytic leukemia (APL), both in its biological characteristics and its response to conventional therapeutic drugs. The aim of our study was to better characterize the efficacy of the combined treatment and to determine molecular markers of clinical outcome. We established a minimal residual disease monitoring based on the high sensitivity of detection of PML-RAR transcripts by polymerase chain reaction (PCR) technology in APL mice. We showed that oncogene-specific PCR-based assays allow, like in patients, the diagnosis, follow-up and prediction of disease evolution. Furthermore, PCR assay was used to assess various tissues and organs for the presence of PML-RAR-positive cells in minimal residual disease free long-term survivors. As expected, majority of mice had no measurable tissue level of PML-RAR demonstrating the efficacy of immunotherapy. However, tracking the oncogene-positive cells reveals for the first time that extramedullary PML-RAR-positive cell reservoirs such as the brain may persist and be involved in the leukemia relapse. We aimed at investigating the immune responses involved in the anti-leukemic effect of the combined immutherapy. To evaluate the...
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Study of differential potential of spermatogonial stem cells via transplantation in vertebrates
Kodedová, Barbora ; Krylov, Vladimír (advisor) ; Pšenička, Martin (referee)
Spermatogonia, or spermatogonial stem cells are necessary to maintain male fertility. In the complex process of ongoing spermatogenesis in the testes these pluripotent stem cells proliferate and differentiate into sperm cells. In 1994 the first spermatogonial transplantation technique was described in rodents to allow the study of male germ cells. The following series of studies of intra- and inter-species transmission of testicular tissue revealed the regenerative capacity of transplanted spermatogonial stem cells and their possible usage. Recently, spermatogonia transplantation systems are developed in many vertebrates making it possible to study the development of sperm as well as artificial production of male and female gametes derived from germ cell donors. The differentiation potential of spermatogonial stem cells enabled the creation of transgenic organisms by genetic manipulation of isolated spermatogonia and subsequent transplantation into a suitable recipient. Spermatogonial transplantation in the future may find their application in regenerative medicine, the treatment of disorders of spermatogenesis, or serve to preservation of genetic stock of endangered species.
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Preparation of expression constructs of phosducine binding partners.
Koláčková, Kateřina ; Obšil, Tomáš (advisor) ; Vaněk, Ondřej (referee)
Key words: SUG1, phosducin, G protein signaling, expression, pGEX-4T-1, pST39 Phosducin is an acidic protein found in cytosol and is involved in G protein signaling within rods of photoreceptors. Phosducin also influences stress-dependent hypertension. One of phosducin's important binding partners is SUG1, a 26S proteasome subunit, which belongs to a family of proteins that have an ATPase activity. This bachelor thesis takes part in a broader research, which tries to clarify physiological functions of the interaction of SUG1 with phosducin. Main goal of this study was to prepare constructs of SUG1 (human isoform 1) suitable for the recombinant protein production in an expression system E. coli BL21 (Rosetta). Gene that encodes SUG1 was inserted into two different plasmid DNAs that allow expression of SUG1 as two different fusion proteins in a prokaryotic expression system. Nucleotide sequences of these constructs were verified using sequencing. Next, expression tests revealed sufficient production of both recombinant fusion proteins. The main result of this bachelor thesis is the successful expression of human SUG1 in E. coli BL21 (Rosetta) cells. This result will enable the development of the purification protocol for the large scale expression of both recombinant fusion proteins as well as the...
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Anxiety and estrangement in Juan Carlos Onetti's works
Krupková, Tereza ; Housková, Anna (advisor) ; Vydrová, Hedvika (referee)
Anxiety and estrangement in Juan Carlos Onetti's works Key words: Onetti, The Well, novel, Eladio Linacero, estrangement, anxiety, loneliness, dreams, Tizón This work focuses on the literary conception of characters in Onetti's works. Specifically, it converges on anxiety, loneliness and estrangement, which are the central components of the author's prose. Onetti's first-fruit, The Well, serves as a starting point. This novel revealed new possible ways of the novel-writing and influenced many other authors. It carries the key issues of Onetti's future work. The novel is sketchy; the images often do not follow each other and they are directed by hidden associations. There appears a new character: urban anti-hero, who escapes from the hostile reality to the fantasy life. The present work features the protagonist of The Well with other Onetti's characters. The part dedicated to the style focuses on the relation between form and content. Onetti's books are about misunderstanding which is reflected in the structure and language. In the final chapter of this work is confronted The Well with The Beauty of the World by Héctor Tizón. Different and in some components similar processes allow to recognize and define Onetti's style and uniqueness better.
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The historical development and present regulation of a child benefit
Boldys, Zbyněk ; Štefko, Martin (advisor) ; Koldinská, Kristina (referee)
Historical development and current regulation of the child allowance in the Czech Republic This thesis describes the development of monetary social allowance paid by state to dependent children. The work revealed that the child allowance is relatively new legal institution first recorded in 1922 in the act that increased incomes of government employees and their family members implicitly. This fact has appeared as a disadvantage of the thesis as it is difficult to define it in comparison to other traditional legal institutions determined, adapted and accepted from Roman law jurisprudence. The thesis deals with the child allowance defined in current legislation as well as with a former education allowances that were of the same form and the current allowances are based on this type. It is abstracted from the chronological outline of the first part as well as the content of the rights and obligations of entities associated with these allowances as well as the common body - the monetary support from the state used for dependent children. An identical object and the rights and obligations arising from it can be found in the content of similar allowances, but due to the extent of the theses there were no possibility to include these in the text. The thesis is divided in two parts. The first part...
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Molecular diagnostics of giant liver fluke (Fascioloides magna)
Siegelová, Veronika ; Špakulová, Marta (referee) ; Kašný, Martin (advisor)
Fascioloides magna (giant liver fluke) is veterinary important endoparasitic helminth parasitizing in a number of vertebrate species (primarily in ruminants), causing them severe health problems, often leading to death. The F. magna adults are mostly localized in the liver tissue of the definitive hosts, where they survive in pseudocysts for a long time and produce eggs. The eggs are released from the pseudocysts via the bile ducts into the gut and then leave the host's body together with faeces. Up to now, the diagnostics of fascioloidosis is primarily based on the dissection of the host's liver. The direct and indirect intravital diagnostic methods could represent an appropriate alternative. Using the intravital diagnostic methods, the parasitosis can be revealed either directly (microscopically) - by discovering the eggs in the host's faeces or indirectly (molecularly) - by isolation of the parasite's DNA and the subsequent PCR. The molecular diagnostic methods also allow the reliable differentiation of F. magna infection from the other eventual trematodosis (caused by e.g. Fasciola hepatica, Dicrocoelium dendriticum, Paramphistomum cervi).
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Methods of molecular and cell biology in the frame of secondary school education
Blaha, Milan ; Ehler, Edvard (advisor) ; Pavlasová, Lenka (referee)
Teaching of cell and molecular biology is often restricted by financial and material limitations as well as a lack of expertise. These problems may be overcome by cooperation with scientific institutions and universities. Some scientists teach select courses under the auspices of high school. Others allow students to fulfill internships at their workplaces. The first aim of this thesis is to describe the characteristics of summer school, wherein the participants are integral for providing feedback on what matters most to them. The second goal of the study is to perform four molecular biology lab lessons in the advanced placement genetics course. The results of a qualitative research conducted among summer school participants have shown the benefits present due to contact that occurs between people of the same genuine interest. Student evaluation of molecular biology lessons performed in the advanced placement course revealed that the lab lessons are feasible and positively accepted by students. The thesis also highlights the importance of cooperation between high school and academia. Key words: molecular biology, DNA, secondary schools, gifted students
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The development of feminist criticism of Charlotte Brontë's novel Jane Eyre: the unusual romance by the Parson's daughter that sparked the literary rebellion
Ondrušková, Andrea ; Beran, Zdeněk (referee) ; Nováková, Soňa (advisor)
In 1847, when Charlotte Brontë was writing Jane Eyre in Haworth parsonage and secretly dreaming of her literary career, not even in her wildest dreams could she have imagined what a life and what a variety of meanings female critics would once give to her first novel, and that one day she would be even studied as one of the female writers who helped to spark the women's literary rebellion and her Jane Eyre would be celebrated as a feminist classic. After all, this is what the fascinating story of this unusual romance has turned out to be. As my thesis has revealed, ever since its publication Jane Eyre has always drawn the attention of female critics. The first woman whose opinion about the novel was publicly heard was the Victorian reviewer Elisabeth Rigby. Interestingly, unlike female critics who came after her, she was not delighted by the appearance of this novel but on the contrary, she felt fully alarmed by it. She condemned it on both moral and religious grounds, disliking Jane's rebellion against the established order, as well as her sympathy towards the poor and the oppressed. Her opinion was nothing unusual concerning the fact that she was both a member of the upper class and a conservative. Yet, what was rather astounding was that as a woman allowed to write, she questioned the propriety of female...
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