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Water and sediment chemistry of fluvial lakes of the Elbe River
Chalupová, Dagmar ; Janský, Bohumír (advisor) ; Jelínek, Emil (referee) ; Benešová, Libuše (referee)
The presented paper aims at the problems of fluvial lakes in middle course of the Elbe River which has been influenced by the human activity since the Middle Ages. The oxbow lakes which are extremely precious ecosystems contributing to the stability of the river ecosystem show not only the changes of the course of the Elbe River. With regard to sedimenting a great amount of pollutants, these lakes also tell about the industrial pollution to which the river was exposed mainly in the second half of the 20th century due to insufficient precautions on industrial sources causing contamination. 5 cut lakes between Hradec Králové and Mělník have been studied within this research. These lakes differ in the intensity of communication with the river, in the distance from significant sources of industrial pollution and in the use of nearby land. The research included morphometric and bathymetric measures, observation of hydrological regime, seasonal measures in the water column and regular analysis of surface water samples. Profiles of sediments in the length that the used technology allowed were sampled, so that older anthropogenic load could be found out. The amount of organic carbon and concentration of selected metal and arsenic (Ag, Cd, Cr, Cu, Fe, Hg, Mn, Pb and Zn) were stated apart from grain size...
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Determination of heparine by SIA with spectrofluorimetric detection
Bár, Ladislav ; Rychlovský, Petr (advisor) ; Nesměrák, Karel (referee)
This thesis was focused on a determination of heparin using sequential injection analysis with spectrofluorimetric and spectrophotometric detection. The principle of determination was based on the interaction of heparin with phenothiazine dye. A decrease of fluorescence intensity of dye in its emission maximum was detected. In the case of spectrophotometric detection a decrease of the absorbance of dye was measured. Azure A, azure B and methylene blue were used as representantive selection of phenothiazine dyes. The determination was performed on a laboratory made SIA apparatus, for which a control software in LabVIEW 7.1 graphical programming was created. Two types of flow configuration for spectrofluorimetric detection were implemented. Type 1: For deionized water as a carrier stream with a injection of heparin and dye zones there were found the following optimal conditions: cdye = 1×10-5 mol dm-3 ; vflow = 2500 µl min-1 ; reaction coil length of 0 cm; injected volume of dye 150 µl and injected volume of sample 150 µl. Dynamic range of calibration curves with an exponential course for the individual dyes in the range of LOQ - 1200, eventually up 1500 IU dm-3 were detected. Limits of detection between 7.6 - 39.1 and the limits of quantification between 58.8 - 124.5 IU dm-3 were found. Type 2: For...
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The leg ulcer
Rohanová, Veronika ; Fröhlich, Filip (advisor)
A crural ulcer is defined as a disorder of skin cover continuity of lower extremities, different morphologies and ethiopathogeneses which cannot be healed within 4 - 8 weeks despite the appropriate therapy. Thus it is not a single diagnostical unit but a complex of symptoms. The healing of chronic ulcerations is a complex and dynamic action whose aim is to renew the eroded skin cover integrity. In general, the course of the healing can be divided into three phases - the inflammation phase (exsudative, cleaning), the proliferation phase (granulation) and the epithelization phase which penetrate each other. Good knowledge of the course and specifications of the particular healing phases is the key for the right choice of the medical therapy and the proper cover material which fully respects the wound needs. The crural ulcer therapy is a medical branch with a wide interdisciplinary field of activity, and the doctor involved should be able to view the patient's crural ulcer as a surgeon, a dermatologist, an internist, a diabetologist, a microbiologist, an orthotist, a physiotherapist, an ergotherapist and a nutrition therapist at the same time. The medical procedure has to be complex, professional and economical enough to make the patient's life as good as possible. A complex approach to the crural ulcer...
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Efficient Gathering of Performance Information on Multicore Systems
Tůma, Tomáš ; Yaghob, Jakub (referee) ; Tůma, Petr (advisor)
Modern multicore processors provide performance counters that export information on various essential aspects of software execution, from instruction decoding to cache utilization. Typically, a processor is capable of counting a small subset from hundreds of different event types, the events themselves can occur almost every processor clock tick. This yields a significant amount of data which is difficult to collect without disrupting the execution itself. The goal of the thesis is to apply compressive sampling - a special method of sampling signals that allows to reconstruct sparse signal from a small number of samples - to the performance counter data.
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Elder abuse and neglect
Haluzíková, Lenka ; Slaměník, Ivan (referee) ; Šulová, Lenka (advisor)
This thesis deals with the subject of elder abuse and neglect (EAN). The author in her theoretical part presents all sorts of aspects of the EAN phenomenon which is broadly interdisciplinary and whose definition and prevalence were not assessed yet. It describes exposures of single types of EAN and symptoms, whose knowledge is necessary for effective detection of particular cases of EAN. Hitherto most of the cases stay concealed because the victims tend to hide the abuse and simultaneously neither the community of specialists nor non-specialists have sufficient awareness about the problem in order to perceive it. In this course an education of the public is crucial but also the author deems necessary to change the attitude of all-society towards the elder people which at the present time seems quite discriminatory. Farther the author attends to a victim and an offender of EAN and their interaction, in this respect she presents risk factors and searches for potential causations of abuse. Detailed knowledge of these causations is crucial for suitable aiming of prevention. For a complex intervention of EAN would be ideal to create multidisciplinary teams of different specialists. But such teams are rather exception worldwide. Among these specialists a psychologist should be present as he can play an important...
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Biological diversity of breast carcinoma cells in relation to the sensitivity on the antitumor treatment
Netíková, Irena ; Veselý, Pavel (advisor) ; Květina, Jaroslav (referee) ; Abrahámová, Jitka (referee)
Biological diversity of cancer cells isolated from breast carcinoma samples has been studied by in vitro exposure to cytotoxic agents. Primary cells from individual primary breast tumours and malignant effusions were in the first phase of the study used for experimental assessment of chemosensitivity/resistance using the standard MTT assay. Cytotoxic effects after short-term cultivation with antineoplastic drugs were evaluated. In several approachable cases relation to the clinical course of the disease was followed; in 4 out of 7 cases good correlation was found. The effect of combination of two cytotoxic drugs on cell viability was studied on EM-G3 cells, showing interesting results with some drug combinations (e.g. combination of paclitaxel and cisplatin resulted in a lower effect than cisplatin alone). Possibility of cell chemosensitisation by tamoxifen pretreatment was tested. Very low chemosensitisation effects of tamoxifen were observed. As a model cell population for diversification development study a new clonal cell line, EM-G3, was established. The EM-G3 line was derived from a primary lesion of human infiltrating ductal breast carcinoma. The EM-G3 represents a unique, spontaneously immortalized clonal cell line, evidently derived from premalignant progenitors of breast carcinoma. The cells are...
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Physiotherapy and It's Role in Impingement Syndrome
Mašek, Jiří ; Vacek, Jan (advisor)
Subacromial impingement syndrome is among the most frequent cases of shoulder pain and dysfunction in adults. It's reffered to as shoulder pain is third only to headache and back pain in freqency seen in the practitioner's office. It's known, SIS is a heterogenous entity, which can be caused by a lot of ethiological factors. These factors can have bad influence of architecture of the subacromial space. The tendons of the rotator cuff are passing trough this space and so that structures are most often affected by degenerative disorders. Clinical history and physical examination play a key role in diagnostics. Provocative manoeuvres (Neer's sign, Hawkin's test, Yocum manoeuvre) seem to be useful for identifying impingement syndrome. If there is a reasonable doubt in diagnostics we can use imaging methods. We should start with the minimally invasive and the most economic method. Commonly used course of action include x-ray, ultrasound and MRI. Well-timed and the most accurate diagnostics enable us to start suitable treatment. The treatment commonly starts with pain-relief drugs (NSAID, subacromial injection of mixture of corticosteroid and local anestethic). The combination of physiotherapy and manual therapy seems to be an effective treatment method. This therapeutic combination is good for improving function...
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The effects of different agro-technologies on the soil physical quality in selected localities in the South Moravian Region
Bažantová, Adéla ; Doležal, Petr (referee) ; Kameníčková, Ivana (advisor)
Farmland is being handled by various the tillage of soil especially for the correct course of soil processes, plant growth and development and of course profit. There are two types of the tillage of soil are their conventional (classical) which involves plowing and minimization tillage, where is excluded plowing. The last few decades, the development the tillage of soil focuses on minimization tillage. This includes loosing up small depth, soil conservation tillage and direct seeding. This thesis is aimed at impact of minimization tillage on selected physical and hydraulic properties of the soil. Sampling undisturbed soil samples was performed on the experimental plot Kozlany by Kopecky rollers (V = 100 cm3) from depth of 0 - 10 cm, during the year 2016. On this plot was used minimization tillage in the form loosing up small depth and direct seeding.
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HPLC separation of p-aminophenol, paracetamol, caffeine and propyphenazone on polar stacionary phase.
Maroušková, Alena ; Sklenářová, Hana (referee) ; Šatínský, Dalibor (advisor)
This thesis deals with finding optimal chromatographic conditions for a separation of p- aminophenol, paracetamol, caffeine and propyphenazon by HPLC. These analytes have different polarity and acidobazic properties. The application of traditional octadecylsilane stationary phases for their simultaneous determination is problematical. The PEG column have proved to be suitable for an efficient separation of the analytes with different polarities, i.e. with different chromatographic behavior. The resulting method brings shortening of analysis time, which is important for analysis of big series . Within the optimization of chromatographic conditions: a wide range of mobile phases has been tested (various organic solvents, various ratio of organic components to water phase components) a various level of acidity of the mobile phase has been tested ranging from 4 to 7 pH units various buffers have been tested behavior of the analytes has been tested under an enhanced temperature two PEG columns with different lengths have been tested Optimal chromatographic conditions for a separation of the analytes mentioned above, as well as the fulfillment of requirements for simplicity, analysis time and high degree of reliability have been found. The final chromatographic conditions: Stationary phase:...
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