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Isolation and Characterization of Protoplasts and their Utilization for Model Membrane
Nováková, Kateřina ; Navrátil, Tomáš ; Šestáková, Ivana ; Langmaier, Jan ; Heyrovský, Michael ; Zámečníková, B. ; Vodičková, H.
Protoplasts are prepared form plant cells by removing their cell walls, which are usually destroyed mechanically or by enzymes. Protoplasts exhibit the spherical shape after removing of their cell walls. They can be isolated from a range of plant tissues: leaves, stems, roots, anthers, and even pollen. Plasmatic membranes surrounding protoplasts can be used as a source of model membranes. Therefore, some parts of plasmatic membranes or subcellular organelle membranes, which contain specific transport systems, can be isolated and integrated to model phospholipid membranes. State of such model membranes can be characterized by electrochemical impedance spectrometry (EIS) and the transported species can be determined with the use of voltammetric methods.
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Gemmotherapy as a method of regeneration (development of a tutorial for students of the Department Health Education)
KOPTÍKOVÁ, Dana
The present Bachelor Thesis deals with gemmotherapy as an alternative method of therapy and regeneration of human organism. The aim of the Thesis is creation of a tutorial and its verification by students of the Health Education course of the Faculty of Education, University of South Bohemia in České Budějovice. The theoretical part includes a detailed systematic review of available Czech as well as foreign specialised literature in view of an effective and sufficient presentation of basic theory and philosophy of gemmotherapy to teachers. The introductory part makes readers familiar with history and use of natural resources for regeneration of the organism; it describes the current situation and presents the facts concerning the arising of gemmotherapy. This is followed by basic principles which are used by gemmotherapy and by distribution of products. A comparison of Czech and world (especially French) gemmotherapy and particular specifics are mentioned as well. An important part is formed of a short chapter from the field of phytosociology of trees. The practical part describes creation of the tutorial - teaching plan and teaching materials. This part is followed by the methodology of research, preparation and course of a questionnaire survey, its results and summary. It is clear from the data acquired that the tutorial created is effective and that it can be used for the teaching of the new regeneration method - gemmotherapy.
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Sensor based on Förster resonance energy transfer with quantum dot
Lišková, Marcela ; Datinská, Vladimíra ; Klepárník, Karel ; Foret, František
Förster resonance energy transfers (FRET) are based on the nonradiative transfer of energy between a donor and acceptor dye in the distance 1-10 nm. With respect to this, quantum dots (QDs) with a high absorbance can serve as suitable donors of energy in FRET. Here, we present architectures of two sensors based on QD conjugates useful in imaging and diagnostics of cancer. One sensor, based on an oligonucleotide conjugate, can be used for the detection of complementary oligonucletide chains. The other was designed for the detection of enzymes inside cells.
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Cells III
Berger, Josef
The book contains 12 full papers and 74 abstracts on cell biology following the review.
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