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Decontamination of the large-scale facility
BURDA, Radek
The theoretical part of the thesis is divided in the five main chapters. First chapter generally defines the term ?contamination?, which is closely related to the decontamination. Second chapter is focused on the microbial contamination, defines infectious agents, the mechanisms of its spread and points out microbes most likely to be misused as a biological weapon. Third chapter of the theoretical part addresses the decontamination itself. It defines the decontamination in general aiming for microbial decontamination, i.e. the biological agent surface and environment removal. Two basic groups of decontamination e.g. physical and chemical procedures are mentioned in this chapter. Fourth chapter presents valid standards describing various types of examinations for the desinfecting preparations efficacy evaluation. The last chapter of the theoretical part covers large-scale facilities, defines them and describes experimental facilities at the Department of large-scale testing in the National institute for nuclear, chemical and biological protection in Kamenna. The aim of the thesis is to create own methodology of the disinfactant efficiency testing with emphasis on the real condition simulation. The decontamination was carried out by means of following methods: aerosol generator method, decontamination by evaporation, manual sprayer and engine sprayer respectively. For the analysis we chose these disinfactants: Chirosan? Plus, Incidin OxyDes, Kohrsolin? FF, Korsolex? basic, Persteril? 36 and Sanosil? Super 25 Ag. The disinfectant efficiency was tested in 30, 60 and 120 m3 facilities. The tested B-agents were: Bacillus anthracis, Bacillus atrophaeus, Escherichia coli, Staphylococcus aureus and Streptococcus pneumoniae. For the decontamination efficiency verification we used a modified surface assay, a modified suspension assay and an agarose assay. The following issues were of concern: We found out that, based on the analysis of different techniques of decontamination, the large-scale facility decontamination is feasible. The most effective method of decontamination appears the manual and engine spraying with the latter more convenient for spaces over the 30 m3 in the matter of time. The decontamination by evaporation was inefficient for the large-scale facility decontamination. Experiments revealed that method based on aerosol distribution is convenient for large-scale facility decontamination. However, the nature of decontaminating preparation has to be considered. All disinfactants tested have shown good bactericidal activity. Nevertheless, only Korsolex? basic a Persteril? 36 have shown sufficient sporicidal activity with equal effect. The disadvantage of the preparation Persteril? 36 is its significant corrosive activity, on the other hand is inexpensive. The preparation Korsolex? basic contains anticorrosive compounds, but is more expensive than Persteril? 36. Our results demonstrated that the most convenient method for the decomtamination of facilities up to the 30 m3 is the manual aerosol sprayer. For facilities of larger volume the engine sprayer decontamination is more convenient. From the preparations tested, only Korsolex? basic a Persteril? 36 are, with respect to advantages and disadvantages, usable in the Department of large-scale testing in the National institute for nuclear, chemical and biological protection in Kamenna.
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Novel Approaches in Electrochemical Determination of Xenobiotic Compounds and in Study of Their Interaction with DNA
Hájková, Andrea
Presented Ph.D. Thesis is focused on the development of analytical methods applicable for determination of selected xenobiotic compounds and for monitoring DNA damage they can induce. The main attention has been paid to the development and testing of non-toxic electrode materials for preparation of miniaturized electrochemical devices and novel electrochemical DNA biosensors. 2-Aminofluoren-9-one (2-AFN) was selected as a model environmental pollutant, which belongs to the group of hazardous genotoxic substances. Its carcinogenic and mutagenic effects may represent a risk to living and working environment. 2-AFN has one oxo group, where the cathodic reduction occurs, and one amino group, where the anodic oxidation occurs. The voltammetric behavior of 2-AFN in the negative potential region was investigated at a mercury meniscus modified silver solid amalgam electrode (m-AgSAE) representing a non-toxic and more mechanically robust alternative to mercury electrodes. This working electrode was subsequently used for the development of a newly designed miniaturized electrode system (MES), which has many benefits as the possibility of simple field measurements, easy portability, and the measurement in sample volume 100 µL. Moreover, a glassy carbon electrode (GCE) was used for further investigation of...
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Units of Physical Quantities and Slightly Mentally Disabled Pupils
Petříková, Tereza ; Dvořák, Petr (advisor) ; Kaslová, Michaela (referee)
The dissertation involves conversion of physical units of such physical quantities as length, weigth, volume, and time. The study is aimed at practical primary schools which means that most pupils are slightly mentally handicapped. The physical quantities are characterized in first chapters. It says why it is difficult for mentally handicapped children to convert physical units and how important it is for their future professions. Next part of this dissertation describes a research which has been carried out to check the pupils' knowledge both theoretical and practical to convert physical units. In the conclusion there is suggested to include this problem more widely into the educational system of practical primary schools.
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Fenomén tůně: úvod do morfologie, hydrologie a limnologie
Pithart, David ; Pechar, Libor ; Hrbáček, Jaroslav
Small size of pools result in large interaction with the surrounding terrestrial ecosystem. 1.Wind protection caused by banks and vegetation results in stable water column. 2. Shading of vegetation restrict the amount of light, reaching the water surface. 3. Inflows of underground water may influence the water chemistry substantially. 4. Bottom processes influence the whole water volume in larger extent compared to large lakes.
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Period copies of Mozart's operas in the music collection of the Premonstratensian monastery in Prague in Strahov
Jonášová, Milada ; Volek, Tomislav (advisor) ; Ottlová, Marta (referee) ; Sehnal, Jiří (referee)
The present dissertation thesis is the result of a several years lasting research of Bohemical Mozart sources, which had been started after my discovery of a hitherto unknown set of scores of Mozart's works in the musical collection of the Premonstratensian monastery at Strahov in 2001. As this set of sources is of a surprising scope and processing them all in detail is going to require some more years of work, this dissertation thesis focuses on one of the key groups of sources, namely those to Mozart's operas. The examination of these sources had to be set into the current context of the international Mozart research. To do this, it was necessary to compare the Strahov copies page-by-page both with the autographs and other relevant period copies. The comparisons were made with autographs deposited in Berlin, Krakow and Paris as well as period copies in Berlin, Dresden, Munich, Karlsruhe, Vienna, Salzburg, Zurich, Brno and Prague. The outcome of this comparative work was then confronted with the latest critical edition, Neue Mozart Ausgabe as well as separately published critical commentaries to the relevant volumes. The first chapter summarizes the known facts about the Strahov Monastery of the Premonstratensian Order as a cultural centre and its musical tradition. A description of the musical collection...
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Huehuetlatolli - moral lessons not only of the old Aztecs
Deylová, Iva ; Křížová, Markéta (advisor) ; Korecká, Zuzana (referee)
The diploma thesis "Huehuetlatolli - moral lessons not only of the old Aztecs" examines this rather unknown genre of Nahua literature from various viewpoints. The original form of these probably only orally transmitted traditions is nowadays known due to the records made by two Spanish Franciscan missionaries Andrés de Olmos and Bernardino de Sahagún. Both of them started showing interest in the indigenous cultures and traditions immediately after arriving in the area of the present Mexico at the beginning of the colonization period. The analysis presented in this work is based on the Volume VI of the book Historia general de las cosas de Nueva Espaa written by Bernardino de Sahagún. The introductory chapters offer a rather general description of Nahua culture and its relationship to the most famous pre-Columbian ethnic group - the Aztecs. This information was largely obtained through the study of texts of both Mexican and foreign scholars such as Ángel María Garibay, Miguel León-Portilla, Markéta Křížová, and others. The text also mentions how the missionaries Andrés de Olmos and Bernardino de Sahagún collected the texts. I also provide arguments why I chose texts edited by Bernardino de Sahagún as a source of this thesis. Additionally, it was also important to classify and mention the main characteristics...
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Current approchaes in the intraday futures trading
Čuraj, Jan ; Málek, Jiří (advisor) ; Fičura, Milan (referee)
This tesis deals with modern approchaes in the intraday futures trading. My focus is on the euro-dollar market. I test and analyse three strategies with using current methods of technical analyses. Thanks to results I explain how to use these strategies in practice to the profitable swing trading and respond on the emerging market on a daily basis.
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Housing Price Determinants in the Czech Republic
Markvartová, Lenka ; Hlaváček, Michal (advisor) ; Šolc, Jan (referee)
Housing market was considered stable until housing prices in many countries after global crises rapidly decreased. Still the development of housing prices and its determinants are unique for every country. The goal of this work is to find the factors that affect the housing prices in the Czech Republic using endogenous models. This type of econometric models enables to explore both short and long term relationships. The theoretical part of the work focuses on housing prices monitoring issues and the specific price development in the Czech Republic in comparison to other countries. Furthermore different level and development of housing prices in Prague are discussed. Due to this fact two types of tests are used - for the Czech Republic as a whole and for Prague separately. The variables housing loans included are based on previous results of Czech as well as foreign studies. Thanks to detected long term relationships an endogenous VECM model can be applied. It confirms bidirectional influence of housing prices and housing loan volume. This relationship could not have been analyzed sofar due to relatively short time series which limit the choice of particular models. My results suggest that growth in housing loan volume causes an increase in housing prices with more than one year delay. This effect...
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Insect cold tolerance and seasonal changes in composition of sterols and tocopherols
URBAN, Tomáš
Presented work focused on researching cold resistance in insects (Pyrrhocoris apterus), especially in qualitative and quantitative composition and seasonal changes in the content of tocopherols and sterols in membranes. Collection of samples, tissue extraction, separation, sample preparation and the final analysis was included. There were detected three different sterols (animal cholesterol, plant sterols: campesterol and sitosterol). Also were detected ?-tocopherol and ?-tocopherol. Volume of cholesterol was in the range of 0.2 to 0.8 mol%, for phytosterols was the range from 1.2 to 15.2 mol%, and for tocopherols was the range from 0.1 to 31.3 mol%. Seasonal fluctuations of sterols and tocopherols were evaluated with a minimum in summer and maximum in winter.
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Residential Building - Lesná
Řeháková, Karolína ; Drobníček, Ladislav (referee) ; Menšíková, Naděžda (advisor) ; Krupicová, Jana (advisor)
The residential building is located in Brno,local part Lesná. The building is designed on a corner place between the streets Dřínová and Tomečkova. The proposal consists of two buildings. Their height arrangement is designed so as to cast a shadow each other or the surrounding buildings. North object exceeds the southern. Mass is based on the efforts to achieve the greatest sunlit room. It is also designed so as to obtain the greatest intimacy on balconies. Balconies also highlight the horizontal breakdown of volume. Residential complex contains thirty different flats floor space and type. The flats are arranged on the axis of north - south, with all habitable rooms are located to the south. In this solution, care was taken and the sunscreening system. It is an outdoor blinds, whose appearance gives the shape of a house. When the proposal was based on the objective of linking objects with the nature of what was to maximize greening space. This helps the inner block, which is used for rest and relaxation. There is also a small cafe designed with outdoor seating. At the same time the grass roof of the smaller objects, when viewed from the upper floors blend into their environment.
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