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Gene Detection in DNA Sequences
Bahurek, Tomáš ; Burgetová, Ivana (referee) ; Martínek, Tomáš (advisor)
This work is about gene detection in DNA sequencies. It explains how the information in DNA is stored, shows preview of various gene prediction methods, describes picked methods and results of testing those methods on real data (genome of bacteria E. Coli K12), compares efficiency of methods with various paramaters and efficiency between methods.
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Estimating the flow capacity of river
Lorenc, Václav ; Dráb, Aleš (referee) ; Duchan, David (advisor)
The bachelor thesis is dealing with the watercourse`s capacity assessment of the river Jihlava on the kilometers 136,5 to 139,5 in the village Maly Beranov. In the chosen locality was made a model of the surface water flow during the flood flow rate Q1, Q5, Q20 and Q100. Based on the gathered information about the area`s utilization, errand and following calculation of the flow, the areas for a potential flood protection were identified. In the problematic locations was suggested to dredge the bottom. In selected sections was made a draft of protective earth dam and concrete wall. According to the draft of flood protection, the model of surface water flow was adapted and the efficiency of flood protection was verified by calculations. All the results are published in clear maps.
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Charge transport in DNA
Drevený, Lukáš ; Mravec, Filip (referee) ; Vala, Martin (advisor)
In this bachelor thesis were investigated charge transport (CT) through a duplexes of DNA by stationary fluorescence spectroscopy, with a fluorescent analogue of adenine - 2-aminopurine (Ap), as fluorescent probe. The aim of study was an influence of changes in the structure of duplexes DNA:DNA or DNA:RNA and effect of the presence of Hg(II) in double-stranded DNA:DNA containing a mutation thymine-thymine in the chain, in the efficiency of charge transfer along the DNA strand. Firstly, in the experimental part was investigated a efficiency of incorporation of Ap into the DNA strand from an excitation spectra and secondly, from the integrals of the emission spectras the quenched factor was calculated. It was found that the Ap can be better incorporated into the chain of DNA:RNA, which is not as rigid as a string of DNA:DNA and thus may be better accepted by the nearby bases in the chain; furthermore, it was found that the photo-induced charge transfer (PET) through the DNA strand sharply drops after exceeding a temperature corresponding with melting point of DNA chain, at which the string of DNA is unraveling. It was also found that the presence of Hg(II) in the chain of DNA:DNA does not adversely affect a charge transfer, but on the contrary, the string is after integration of Hg(II) into the chain more stable and the melting point is shifted to higher temperatures. In this thesis it was proved that photoinduced charge transfer is very sensitive process and could be used for detection of even tiny changes in the structure of nucleic acids.
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The potential of deep seabed polymetallic nodules
Tormová, Lucia ; Dvořák, Antonín (advisor) ; Hadrabová, Alena (referee)
The deep seabed polymetallic nodules are one the major types of deep-sea ore resources. Oceanic deposits of these raw materials represent a potential source of numerous metals that are in short supply on the land-base deposits at the nearest future. The process of geological survey to the deep ocean floor is considered to be an onset of a quantitatively new stage in the process of mineral resource extraction. Continuation of the on-going work on polymetallic nodule deposits within the Clarion-Clipperton Zone is regarded as a serious task to be undertaken over a period until the year 2025, with a due consideration to advance in designing of an efficient mining unit and application of modern processing technologies for extraction of major metals : manganese, nickel, cobalt and copper. A prerequisite of a rational management of oceanic resources is the preservation of natural oceanic environment. Considered is the extraction's profitability of a concrete deposit within the Clarion-Clipperton Zone, which is the exploration area of the IOM, with annual production scale of 2.2 Mt of wet nodules for the year 2025.
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Mechanism of tumor development and its influencing by ellipticine
Parisová, Martina ; Stiborová, Marie (advisor) ; Moserová, Michaela (referee)
Ellipticine (5.11-dimethyl-6H-pyridate [4,3-b] carbazole) is a powerful anti-cancer agent, exhibiting multiple mechanisms of action. This work describes the causes of cancer processes and summarizes the main pharmacological mechanisms and cytotoxic effects of ellipticine together with the results found in our laboratory indicating, a new mechanism of ellipticine action. Cytotoxic and mutagenic activity of ellipticine is attributed to its two mechanisms of activity ellipticine intercalation into DNA and its effectivity to inhibit topoisomerase II. Ellipticine also forms covalent DNA adducts after its oxidation with cytochromes P450 and peroxidases. Cytochromes P450 oxidize ellipticine up to five metabolites, of which 13- hydroxyellipticin, 12-hydroxyellipticin and N(2)-oxide of ellipticine are responsible for formation of two major DNA adducts. In the case of peroxidases, ellipticine is oxidized to a radical producing the ellipticine dimer and a minor ellipticine metabolite, the N(2)-oxide of ellipticine. Because of the high efficiency of ellipticine and its derivatives against various types of cancer, this coumpound is studied in detail. Its utilization for drug tangeting is a challenge for further study.
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Laboratory options in diagnostics of bacterial meningitis
Fárová, Marcela ; Nyč, Otakar (advisor) ; Hobstová, Jiřina (referee)
The mutual companson of the methods used for the diagnostics of bacterial meningitides was the aim of this work. The bacterial meningitis is a very serious disease and its laboratory diagnostics should include such a spectrum of the examinations, which guarantees the highest posibility of discovering the pathogens. The efficiency of latex agglutination, classic bacterial cultivation and peR of Neisseria meningitidis, Haemophilus influenzae and Streptococcus pneumoniae was compared. The peR enables the direct prove of DNA for the pathogens mentioned above. The results confirmed the contribution of peR that is rapid, specific and sensitive. It was proved in 5 cases, where the peR was positive, while the other tests were false negative. Powered by TCPDF (www.tcpdf.org)
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Phylogenetic analysis of Rieske dioxygenases large subunits genes in soil contaminated with jet fuel
Ptáček, Jakub ; Bořek Dohalská, Lucie (advisor) ; Poljaková, Jitka (referee)
The former military air-base Hradcany is among the most contaminated with organic pollutants localities in Czech Republic. Main cleanup strategy in the area is the bioremediation taking advantage on the natural potential of the autochthonous soil microorganisms to evolve catabolic pathways for in situ degradation of the pollutant. The diversity and abundance of the pathways, as well as the specificity and activity of the encoded enzymes are priority biotic factors determining the bioremediation efficiency. Main task of this work was to analyze the bacterial diversity in jet fuel contaminated soils based on key catabolic genes encoding the Rieske non-haem iron dioxygenases of the toluene/ biphenyl oxygenase branch. High molecular soil DNA was extracted and the sequences encoding catabolic genes were selectively enriched by hybridization to biotinylated oligonucleotides on magnetic microbeads with covalently bound streptavidin. Fragments of the genes for the -subunits of Rieske non-haem iron oxygenases were amplified and analyzed by restriction analysis, cloning and sequencing. Their evolutionary histories were inferred using the Neighbour-Joining and the maximum likelihood methods. The catabolic genes diversity in the actively bioremediated and highly polluted soil HRB was compared with the diversity in the...
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Effect of jumping training on the postural functions of the spine
KEPKOVÁ, Jana
For my bachelor thesis I chose the theme ?The Effect of Jumping Exercise on Postural Functions of the Spine.? Jumping is safe and gentle aerobic exercise on mini trampolines, which has beneficial effect on virtually all systems of the human body. Speaking about the deep stabilization system, we mean muscle interplay mm.multifidi, diaphragm, pelvic floor and abdominal muscles, which together regulate intra-abdominal pressure. This muscular interplay which ensures stabilization of the spine is activated throughout all movements. Its dysfunction is one of the most significant factors causing back pain. The jumping exercise strengthens these important muscles and improves the overall stability of the body. The objective of the research was to assess the deep stabilization system with respect to kinesiology and kinesiotherapy and to find out whether and how the jumping exercise influences the postural functions of the spine. The data for the thesis were collected by means of qualitative research, by the method of case study and the technique of personal case study. The examination of probands includes a brief history, palpation and visual examination, the examination of deep stabilizing system by functional tests and then also the testing by L.A.S.A.R posture device. Based on the comparison of the initial examination and the final examination, which followed after 10 weeks of jumping exercise, a previously set research question was answered. The thesis involves the theoretical part, which describes the jumping exercise, anatomy, examination of the deep stabilization system and its importance for our body. The practical part involves objectives of the thesis, research questions, the description of methodology, discussions with the authors from which I drew information and primarily, processed results in the form of case reports. The research results show that there were clear positive effects of the deep stabilization system and other structures in all probands. On the jumping lessons the probands were regularly forced to activate the muscles of the deep stabilization system, which resulted in reduction of back pains, in reduction of knee pain in one case and the overall stability of the body improved. The results of the thesis could be used in therapeutic procedures of physiotherapists, for the purposes of further research, in teaching and also in the implementation of more efficient jumping lesson, etc.
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efficiency of the bottom elements on the supercritical flow energy dissipation
Knoflíček, Jakub ; Haluza,, Miloslav (referee) ; Šulc, Jan (advisor)
The thesis is dealing with comparing dissipation of the kinetic energy water flow using various bottom elements based on model research. There is missing information about efficiency of different kinds of bottom elements. This absence does not allow to compare different modification of the bottom of stilling basins. Within experimental research were measured depths in different variants. Based on the results of model tests are evaluated and tabulated sorted variants of the combat efficiency of the kinetic energy water flow. The results will be used for the efficient and economic design of additional alterations of stilling basins.
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