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Original title:
Transgenerational effect in \kur{Taraxacum brevicorniculatum}: test of a novel method of experimental plant DNA demethylation and its practical application in exploring the impact of maternal competition on progeny phenotype
Authors: DVOŘÁKOVÁ, Hana
Document type: Master’s theses
Year: 2016
Language: eng
Abstract: Spray application of 5azacytidine on established plant seedlings was tested for its demethylating efficiency, as it represents a novel method for plant experimental demethylation with a potentially lower negative impact on plant development compared to the traditional application of the demethytaling agent through germination of seeds in its solution. Further, the 5azacytidine spray application was used in practice to erase the epigenetic memory in offspring of Taraxacum brevicorniculatum plants from different competitive conditions. The impact of parental competition on the juvenile phenotype was estimated by measuring growth related traits, while the experimental demethylation allowed for evaluating the significance of DNA methylation marks in bioticaly induced transgenerational effects in T. brevirorniculatum.
Keywords: 5-azacytidine; competition; experimental DNA demethylation; seed mass; transgenerational effect
Citation: DVOŘÁKOVÁ, Hana. Transgenerational effect in \kur{Taraxacum brevicorniculatum}: test of a novel method of experimental plant DNA demethylation and its practical application in exploring the impact of maternal competition on progeny phenotype. České Budějovice, 2016. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/44454
Permalink: http://www.nusl.cz/ntk/nusl-262680
The record appears in these collections:
Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Master’s theses
Authors: DVOŘÁKOVÁ, Hana
Document type: Master’s theses
Year: 2016
Language: eng
Abstract: Spray application of 5azacytidine on established plant seedlings was tested for its demethylating efficiency, as it represents a novel method for plant experimental demethylation with a potentially lower negative impact on plant development compared to the traditional application of the demethytaling agent through germination of seeds in its solution. Further, the 5azacytidine spray application was used in practice to erase the epigenetic memory in offspring of Taraxacum brevicorniculatum plants from different competitive conditions. The impact of parental competition on the juvenile phenotype was estimated by measuring growth related traits, while the experimental demethylation allowed for evaluating the significance of DNA methylation marks in bioticaly induced transgenerational effects in T. brevirorniculatum.
Keywords: 5-azacytidine; competition; experimental DNA demethylation; seed mass; transgenerational effect
Citation: DVOŘÁKOVÁ, Hana. Transgenerational effect in \kur{Taraxacum brevicorniculatum}: test of a novel method of experimental plant DNA demethylation and its practical application in exploring the impact of maternal competition on progeny phenotype. České Budějovice, 2016. diplomová práce (Mgr.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta
Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/44454
Permalink: http://www.nusl.cz/ntk/nusl-262680
The record appears in these collections:
Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Master’s theses
Record created 2017-01-20, last modified 2023-01-15