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ELECTRON BEAM REMELTING OF PLASMA SPRAYED ALUMINA COATINGS
Matějíček, Jiří ; Veverka, J. ; Čížek, J. ; Kouřil, J.
Plasma sprayed alumina coatings find numerous applications in various fields, where they enhance the properties of the base material. Examples include thermal barriers, wear resistance, electrical insulation, and diffusion and corrosion barriers. A typical structure of plasma sprayed coatings, containing a multitude of voids and imperfectly bonded interfaces, gives them unique properties - particularly low thermal conductivity, high strain tolerance, etc. However, for certain applications such as permeation barriers or wear resistance, these voids may be detrimental.\nThis paper reports on the first experiments with remelting of plasma sprayed alumina coatings by electron beam technology, with the purpose of densifying the coatings and thereby eliminating the voids. Throughout the study, several parameters of the e-beam device were varied - beam current, traverse velocity and number of passes. The treated coatings were observed by light and electron microscopy and the thickness, structure and surface morphology of the remelted layer were determined and correlated with the process parameters. Based on the first series of experiments, the e-beam settings leading to dense and smooth remelted layer of sufficient thickness were obtained. In this layer, a change of phase composition and a marked increase in hardness were observed.\n
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The Role of a Nurse in Care of a Patient with Mononucleosis
LAŽANSKÁ, Soňa
Theoretical background Infectious mononucleosis belongs to relatively widespread viral infectious diseases in the Czech Republic. Epstein-Baar virus (EBV) participates in occurrence of the infection by 80 %, infectious mononucleosis may more rarely be caused by cytomegalovirus. Each individual factually suffers from infection caused by EBV during his/her life, however its course is unapparent in most cases. An affected individual or an individual not affected, who is a virus carrier may be considered the source of the infection. Infectious mononucleosis usually affects children and teenagers, in older individuals the infection is quite rare. The disease might start rapidly or gradually with prodromes like headache, sore throat, nausea, fatigue and perspiration. Infectious mononucleosis is often confused with other diseases like e.g. tonsillitis, or more serious progress of flue, particularly for the symptoms accompanying the disease. Fever, lymphadenopathy and hepatosplenomegaly are the most frequent symptoms. The disease may also cause numerous unpleasant complications, particularly hematologic, neurologic, cardio-pulmonary and autoimmunity complications. A nurse plays a very substantial role in client care. It is very important for a nurse to have sufficient knowledge and to know the right procedure of all interventions he/she meets within diagnostics and treatment of infectious mononucleosis. These are particularly biological material collection, per os administration or intravenous administration of medicines. A nurse should also be familiar with the Priessnitz compress application technique. In non-self-sufficient clients a nurse is also responsible for client's hygiene. A nurse has an important role in client education providing information on dietetic measures. A nurse has to lead a client to adherence to the prescribed diet and body calm. Everything depends on client's current condition. A client should also avoid mental strain during convalescence. We should note that not all forms of infectious mononucleosis require hospitalization. The aim of the thesis The aim of the thesis was to find out the role of nurses in the care of patients with infectious mononucleosis and also to find how satisfied patients with the nursing care and education are after going through mononucleosis. This was why the following research questions were chosen: What is the role of a nurse in care of a patient with mononucleosis? How satisfied are patients with the nursing care after going through mononucleosis? How satisfied are patients with education in diet regime adherence after going through mononucleosis? Applied Methods Qualitative research was chosen for finding out the required information. The information was gained by means of deep interviews with nurses working at the infectious department of České Budějovice Hospital and with clients after going through infectious mononucleosis. All the interviews were recorded on a voice recorder and then transcribed. The transcribed interviews were subject to detailed analysis by the paper and pencil method with open coding. Results Six categories were set upon analysis of the interviews with nurses: Awareness of the infectious mononucleosis disease among nurses, Nursing care of a client with infectious mononucleosis, Regime measures, Information on dietetic measures, Recommendations after infectious mononucleosis and Education of a client with infectious mononucleosis. Five categories were set upon detailed analysis of the interviews with the clients: Recommended treatment, Nursing care, Recommended measures, Methods of information communication and Information sources for clients. Subcategories, in which the key data are coded, are assigned to the individual categories.
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Infection risks of nursers at the inpatient wards
KUČÍRKOVÁ, Alena
Theoretical backround Working as a nurse is one the most human jobs, by serving to people, taking care and preserve health of a human, which is considered to be the highest value of human life for centuries. The aim of nurses is to take care of a human, to mitigate his or her symptoms and to comfort the life till its end if there is no possibility for full recovery. While they do previously described most of them are not aware of a need to take into account also their own health and especially protection against all dangerous microbes. In my bachelor thesis I analyse infection diseases sometimes also called occupational infections which are the most dangerous for nurses. All occupational infections are stated here together with their descriptions. In following parts of this thesis there are hygiene requirements on the workspace and finally in the last section hygienic-epidemic actions in a health care system are described. Objectives of this thesis Objective 1 Determine whether nurses comply with preventive actions against risks of infections. Objective 2 Determine whether nurses know risks of infections at patients departments. Objective 3 Determine whether nurses know how to defend their spread. Research questions Research Question 1: How do nurses protect themselves against risks of infections? Research Question 2: What information do nurses have about risks of infections in health care system? Research Question 3: In which way do nurses prevent the spread of infection to a de- partment? Methodology The bachelor thesis presents a qualitative research. Methods and techniques of a data collection, which we used in the work were participant observation and semi-structured interviews. The survey was carried out with 6 respondents in anesthesiology and resuscitation departments, surgical and internal intensive care units and intermediate care unit at Nemocnice České Budějvice a.s. The research method of an active observation was used during the survey. We observed certain facts that were recorded during the observation to a observation sheet (Appendix 4) and then transcribed and analysed. Data were collected using semi-structured interviews. The interview was conducted with the same respondents who were observed and recorded in a written form and then overwritten (Appendix 6). Results The results of the observations in the research study showed that nurses unfortunately do not comply with barrier nursing techniques as much as they should. The only thing that they fully comply with the overall finish, wear prescribed clothing and collection of biological material according to hygienic principles. Worse situation was in the using of protective equipment, disinfection and hand washing and eating food, where it did not comply 100%. In the next part of the research we focused on interviews with the observed respondents, the purpose was to find things that could not be identified by the only observing. The results showed that nurses do not even have much theoretical knowledge as regards to a occupational infection, or an injury treatment procedure when infected by an object. Conclusion The survey showed that while most nurses have a clue about good practices and knowledge of the occupational infections and hygienic-epidemiological measures ,but unfortunately theory is different from practice in which these things too oblivious. It emerged that unfortunately practice is different than the theory that we learn at universities and colleges.
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FAUST AND JOB´S
Slezáková, Kateřina ; ULLRICHOVÁ, Daria (advisor) ; SÍLOVÁ, Zuzana (referee)
This thesis examins the nature of Faust’s inner ambivalence in the context of modern times. It tries to identify the basic traits leading to the feeling of emptiness. The authoress uses comparing the dramatic character of Faust with his biblical prototype of Job and one of the most successful entrepreneurs today Steve Jobs. Based on the comparison she contemplates the validity or mutation of the drawing theses nowadays. In the second part the authoress reflects the production of the project of Faust. Due to her specialization in the field of dramaturgy she pursues especially the process of the selection of particular dramatic situations, adjustments of dramatic text and the choice of suitable staging means.
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Marketing plan
Nosálová, Lucie ; Štůsek, Jaromír (advisor) ; Ladislav , Ladislav (referee)
The goal of this thesis is to detect errors in the management of small family business, which basically does not use marketing. Following this finding should be to build an effective marketing plan. The diploma thesis is divided into theoretical part, which forms the basis for the analytical part. In the analytical part is described the company and there are also detect errors in the management that are caused by faulty marketing. To achieve results it is important to use several different types of analysis on which it is necessary to set new marketing goals, strategies, changes and projects. The aim is to provide business information and convince him that use of good marketing communication should lead to improve management efficiency and ultimatem increase profitability of the company, without the need to break away from the corporate goal of maximum efforts to build a pro-customer oriented service.
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Managerial skills
Červinková, Barbora ; Kříž, Josef (advisor) ; Zdeněk, Zdeněk (referee)
The theoretical basis of this thesis explains the terms closely associated with the management and managerial skills. Introductory part relates to approach the concept itself and the executive management, we are further broken down managerial functions, which are continuously illuminates to describe other skills, such as communication, goal setting, skills and coaching. The main point is to approach the concepts of soft and hard skills that managers should possess, or which should in the course of his practice to learn. Only by careful control of both of these groups of skills can lead to good organization. For a clearer explanation of the managerial skills included graphic view, which approximates the distribution of skills. In the second part, a practical part, described the situation with the existing skills of managers in the selected company. Thanks to the questionnaire, it is possible to measure the current level of acquired skills of managers at three levels and can be evaluated, what is the interest of improving individual skills, or whether managers feel sufficiently qualified for their job positions.
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Freezing technology of bull sperm in relation to its survivability and fertilization ability
Doležalová, Martina ; Stádník, Luděk (advisor) ; Jiří, Jiří (referee)
The aim of optimalization the insemination doses production is to provide the highest fertilization ability of spermatozoa during the demanding proces of processing fresh semen and its subsequent cryopreservation. Temperature changes causes spermatozoa damage during the cooling and freezing. Spermatozoa is exposed to cold shock and many others limiting factors, which leads to cell death and therefore to decline of fertilization ability of thawed insemination doses. For increasing spermatozoa resistance, exactly the plasma membrane resistance against cold shock was fraction of egg yolk LDL cholesterol (low density lipoprotein) at various concentrations into the comercially produced diluents added. It is believed that LDL acts possitively to plasma membrane and helps to maintain the fertilization ability of spermatozoa after thawing. Following step in the proces of insemination doses production is slow cooling of diluted semen and equilibration, when the straws are store at cooling box for 30 minutes to 240 hours. This period is necessary to penetrate of certain diluent components into the spermatazoa also maintain the balance between their intracellular and extracellular concentration. Also important is subsequent freezing temperature gradient of insemination doses. The most suitable freezing method is based on computer controlled temperature decline in freezing chamber which allows the precise control of ice crystals formation that could tear and kill the cell.
During 2012 to 2016 was repeatedly collected semen from the group of breeding bulls (n = 27, Holstein and Czech Fleckvieh breed) at AI centre. Semen which fulfill the standard entrance conditions in first step was evenly into several parts divided. For dilution the three types of comercially diluents AndroMed, Bioxcell and Triladyl with and without LDL addition were used. Into the diluents AndroMed and Bioxcell the concentration of LDL 4 %, 6 % and 8% into the dilent Triladyl 6 %, 8 % and 10 % was added. Diluted semen was filled into the glass capillares with volume 0,1 ml and temperature +4 °C. Subsequently the sample was placed to cold bath (0°C) for 10 minutes. Then the volume of capillare with physiological solution (37 °C) was mixed and for next 120 minutes was incubate. The effect of cold shock to proportion of live spermatozoa was evaluated by using Eosin and Nigrosine staining technique during heat test of spermatozoa survivability after spermatozoa heating and after 120 minutes of incubation.
The more suitable semen diluents which provide the higher spermatozoa resistance against cold shock were AndroMed and Bioxcell. Together the possitive effect of LDL addition into the diluents to lower decrease of proportion of live spermatozoa during heat test was found (P<0.05). The most suitable LDL concentration which had a favorable influence at spermatozoa resistance against cold shock was 6 % in diluent Bioxcell. Values of the proportion of live sperm were higher at the beginning of the heat test (+1.31% to + 3.2%) and after 120 minute incubation (+5.82% to +8.41%) compared to other diluents with and without addition of LDL.
In the next step the process of equilibration was optimized, is an important part of insemination doses production. The effect of the length of equilibration for subsequent fertilization ability of spermatozoa was evaluated using spermatozoa motility based of CASA and proportion of live spermatozoa after thawing and during heat survival test lasting 120 minutes (37 ° C). Suitable semen was diluted by comercially used diluent AndroMed based on soya lecithin, filled into the straws (0.25 ml), cooled and equilibrated in cooling box for 30, 120 and 240 minutes and freezed in programmable freezing box applying four types of freezing curves differing in temperature rate decline. There was used standard and by producer recommended 3. phase freezing curve, then 2. phase freezing curve, and 3. phase freezing curve with slower as well as rapid decline of temperature rate in freezing chamber, compared with standard freezing curve. The highest spermatozoa motility was found using 240 minutes of equilibration by +2.72% and +4.58% compared to other lengths of equilibration (P <0.05 to 0.01). The highest proportion of live spermatozoa was found using 120 minutes of equilibration (+6.87 % and +8.68 %). The highest average spermatozoa motility during heat test after thawing was achieved by using 2. phase freezing curve (from +2.97% to +10.37%, P <0.05), also in the proportion of live spermatozoa (from + 4.37% to +8.82%, P <0.01). When evaluating interaction between the length of equilibration and freezing curve (standard 3. phase and 2 . phase freezing curve), the highest average spermatozoa motility and proportion of live spermatozoa using 240 minutes of equilibration by both freezing curves was reached, there was no statistically significant differences. As well as, in all evaluated parts of this study the individual differences between ejaculate of bulls and within semen from one bull (P <0.05) as secondary effect were found.
To maintain good fertilization ability of semen during cryopreservation is necessary to increase the spermatozoa resistance against cold shock using addition of correct concentration of LDL into the commercially used diluents AndroMed and Bioxcell. Subsequently the fertilization ability of insemination dose is influenced by cooling, the length of equilibration and freezing. The length of equilibration 120 minutes and more as well as gentle way of freezing according to freezing curve, which ensures a gradual decrease of temperature in freezing chamber provided the higher average spermatozoa motility and proportion of live spermatozoa.
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Influence of meadow management on the migration behavior of selected species of butterflies
Bubová, Terezie ; Langrová, Iva (advisor) ; Farkač, Jan (referee)
Migrations are the key process and one of the basic biological features, which allow survival of endangered species in fragmented landscape. In the last decades, agriculture intensification, industry and urbanization caused that number of natural butterfly habitats has significantly decreased. The localities originally suitable for butterflies are currnetly converted to farmland or building plots. This PhD thesis aimed to find a compromise solution of meadow management, which should allow a commercial use and concurrently do not jeopardize the continued existence of endangered or threatened species. The main idea of this study was in the intentional emigration causing in the middle of flight period, which should lead into resettlement of the population to another (potentially more suitable) locality. Possibility of aimed manipulation with butterfly emigrations would be considered as a major breakthrough in their protection.
The investigated species Phengaris nausithous (Bergsträsser, 1779) and Phengaris teleius (Bergsträsser, 1779) are both considered to be flagship species for protection of European butterflies at open meadows. This study is based on research, which was carried out in 2013-2015 at 17 patch in localities Dolní Labe, Czech Republic. In above mentioned years, one selected meadow was mowed in the middle of flight season and the emigrations were then monitored using mark-release-recapture method. Results of population and migration characteristics were calculated in MARK 8.1 software. Subsequently, the effect of mowing on emigration (comparison of emigrations from the meadow before and after mowing) was evaluated using Statistica 13.
The hypotheses of this study were based on the assumption, that the loss of resources after well-timed mowing should increase the activity of adult butterflies. During the monitoring, number of emigrations was increased only in flight season 2015. Unfortunately, the statistical evaluation did not show significant effect of mowing on migrations. On the other hand, the hypothesis dealing with colonization of nearest meadows was confirmed.
Effect of intentional emigration caused by management interventions was unfortunately not proved. However, we found out, that even though the population at one mowed meadow decreased clearly, the metapopulation size at the locality remained more or less stable.
At investigated (mowed) meadow, there was observed a sharp decline of P. nausithous abundance. On the other hand, P. teleius population was only slightly reduced. It is therefore conceivable, that P. teleius are not as susceptible to innapropriate term of mowing. The results obtained in our research conclusively displayed no possible positive effect of mowing in the middle of flight season and a necessity of suitable management maintenance at localities inhabited by Phengaris butterflies.
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Antioxidant, anti-proliferative and immunomodulatory effect of fruits, vegetables and mushrooms in vitro
Doskočil, Ivo ; Havlík, Jaroslav (advisor) ; Orsák, Matyáš (referee)
Recently there has been an increasing interest in discovering of new species of plants and mushrooms which have antioxidant or anti proliferative activity. The interest is caused by the fact that these species have medicinal and food utilization. These properties of plants and mushrooms can be used when dealing with many diseases which may be connected with oxidative stress (inflammatory bowel disease, cardiovascular disease, hypertension, and tumour disease, etc.).
The thesis propounds characteristics of in vitro antioxidant and anti proliferative activity of 19 types of juices and 28 methanol extracts of fruits and vegetables, which are common parts of a diet. In the thesis there are also characteristics of 13 ethanol extracts of edible mushrooms of the order of Polypore (Popyporales). Antioxidant activity was quantifie by 2, 2 diphenyl 1 picrylhydrazyl (DPPH), oxygen radical absorbance capacity (ORAC), and inhibition of nitric oxide (NO) production. Cytotoxicity was measured by MTT (3 (4,5 dimethylthiazol 2 yl) 2,5 diphenyltetrazolium bromide. Immunomodulatory activity was determined by an assay of phagocytic activity of human neutrophil granulocytes. Further a total phenolic content (TPC) and total beta glucan content were investigated; these are considered to be the potentially active constituents participating in mentioned activities.
From the obtained results it is evident that juices (TPC = 1603.2 mg GAE/L; ORAC = 438.5 umol TE/g)
and a bilberry (Vaccinium myrtillus) fruit extract (ORAC 836.6 umol TE/g; DPPH = 404.6 umol TE/g) showed
the highest antioxidant activity of all tested samples of fruits and vegetables. Capsicum (Capsicum L.)
juices (TPC = 642.1 mg GAE/L; ORAC = 127.9 umol TE/g) and a radish (Raphanus sativus L.) extract
(ORAC 724.5 umol TE/g; DPPH = 52.2 umol TE/g) also proved to have the high values of antioxidant activity. From the tested fruits and vegetables the following inhibited to produce a nitric oxide: onion (Allium cepa L) juices (lowered the NO production by 57%), tangerine juices (Citrus reticulata Blanco)(by 52%), broccoli (Brassica oleracea var. botrytis italic) extract (by 21%), and orange (Citrus sinensis Pers.) extract (by 10%). Concerning edible
mushrooms, Lentinus tigrinus (Bull.) Fr. (TPC = 216.2 umol GAE/g of extract), Ganoderma lucidum (Curtis) P. Kras (TPC = 257.9 umol GAE/g of extract), and Royoporus badius (Pers.) A.B. De (TPC = 257.8 umol GAE/g of extract) presented the highest phenolic content. Sparassis crispa (Wulfen) Fr. (117.4 mg/g of extract) had the highest content of beta glucan. Substantial effect on phagocytic activity of granulocytes was noticed in connection with Neolentinus lepideus (Fr.) Redhead & Ginns, Polyporus squamosus (Huds.) Fr., and S. crispa. In connection with the latter of mentioned mushrooms, a moderate inhibitory effect towards HT 29 human colorectal adenocarcinoma cell line
(IC50 = 107 ug/ml of extract) was noticed.
The results indicate that the tested plants and mushrooms can be beneficial when reducing the negative effects of oxidative stress. The oxidative stress has been recognized as a contributing factor to a whole range of diseases and the reduction of the oxidative stress may lead to the decrease in the possibility of the diseases´ progression. The results show the possible favourable effects on the human health. When evaluating the results, the following fact should be taken into consideration; the in vitro tests and screenings are considered to be the first phase of systematic research of the effects and serve for the choice of respondents for further detailed studies.
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Total contents and speciation of arsenic and selenium in plants growing in soils with different physico-chemical properties
Tremlová, Jana ; Száková, Jiřina (advisor) ; Vymazal, Jan (referee)
This dissertation thesis deals with uptake, accumulation and transformation of arsenic and selenium in plants growing on soils with different chemico-physical soil properties. The contents of arsenic were investigated in 99 wild plant species, coming from 27 different families and 6 different vegetables growing in arsenic contaminated soils. The results suggest that there are species-specific mechanisms which protects certain plants from the excessive uptake of arsenic from the soil. On the other hand, there are plants using a strategy of accumulation, which theoretically may increase the risk of arsenic entry into the food chain, whether through grazing cattle and wildlife or through consumption of vegetables growing on arsenic contaminated soils. The most prevalent arsenic compounds are AsV and AsIII. Other arsenic compounds occure as minor species. An important finding was detection of arsenobetaine in Plantago lanceolata L. and Carex praecox Schreb ..
The contents of selenium were examined in 73 species of wild plants, coming from 29 different families. The results suggest that naturally low levels of selenium in the soil of selected locations along with a low capability of selenium uptake via collected plant species lead to the low content of selenium in plant biomass which may contribute to selenium deficient throughout the food chain. Foliar application of selenate on wild plant communities and on some types of vegetables in our case Brassica oleracea var. italica can have a positive impact on increasing the selenium content in the aboveground biomass of these plants and by extension, increase the selenium content in the human diet. Dominant selenium compounds in the aboveground biomass of the analyzed plants were SeVI and selenomethionine, with variable proportion of other commonly occurring organic selenium compounds, which is mainly affected by plant species.
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