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Antioxidant enzymes response to abiotic stress. Impact of decreased cytokinin level.
Lubovská, Zuzana ; Wilhelmová, Naďa (advisor) ; Prášil, Ilja (referee) ; Kočová, Marie (referee)
Charles University in Prague, Faculty of Science Department of Experimetnal Plant Biology Ph.D. study program: Plant physiology and anatomy Abstract Antioxidant enzymes response to abiotic stress. Impact of decreased cytokinin level. Zuzana Lubovská Supervisor: RNDr. Naďa Wilhelmová, CSc. Supervisor-consultant: RNDr. Helena Štorchová, CSc. Praha, 2015 The response of the antioxidant enzyme system to drought, heat and a combination of these stresses was followed in tobacco plants overexpressing cytokinin oxidase/dehydrogenase1 (CKX1) under the root-specific WRKY6 promoter (W6:CKX1 plants) and under the constitutive 35S promoter (35S:CKX1 plants) and in the corresponding wild type (WT). CKX1 over-producing lines developed a bigger root system, which contributed to their stress tolerance. The high stress tolerance of 35S:CKX1 plants was also associated with a dwarf shoot phenotype and changed leaf morphology. The genes for chloroplastic antioxidant enzymes, stroma ascorbate peroxidase (sAPX), thylakoid ascorbate peroxidase (tAPX) and chloroplastic superoxide dismutase (FeSOD), which are responsible for scavenging of reactive oxygen species produced via electron transfer during photosynthesis, were all strongly transcribed in control conditions. All the tested stresses down-regulated expression of these genes...
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Pupil`s preconceptions about heat and temperature
Schamberger, Jiří ; Mandíková, Dana (advisor) ; Kolářová, Růžena (referee)
Title: Pupil's preconceptions about heat and temperature Author: Jiri Schamberger Department: Department of Physics Education Supervisor: RNDr. Dana Mandikova, CSc., Department of Physics Education Advisor: Mgr. Petr Kacovsky Abstract: This thesis deals with the preconceptions of students in the field of heat and temperature. First, here is an overview of textbooks for elementary and secondary schools with topics concerning heat and temperature, and an overview of czech and foreign literature on the subject. The thesis also contains a survey conducted among primary school pupils and their corresponding grades of grammar schools and overview of misconceptions obtained on the basis of this survey. The final part contains two worksheets created to better understand the subject matter of the heat and temperature. Keywords: Preconceptions, misconceptions, heat, temperature, primary and grammar school pupils
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Influence of Bottom Preheating in Rework of Assembled PCB
Čechák, Ondřej ; Bžoněk, Tomáš (referee) ; Starý, Jiří (advisor)
This bachelor's thesis introduces the problematics of physical mechanisms of heat transfer and the correlative mathematical formulas and the use of mentioned problematics in the technology of bottom preheating in the rework of assembled printed circuit boards. It explains the importance and reasons of use of this technology, not merely in the professional production. It focuses on expert analysis of the appliances based on their properties and clarifies the differences in basic types of the devices. The emphasis is then laid on a practical testing of the technical parameters of the preheating devices as well as on an experimental design and a practical testing of a local bottom printed circuit board preheater.
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System Proposal for the Use of Low-potential Heat
Kučera, Roman ; Bartoň, Jan (referee) ; Mastný, Petr (advisor)
This bachelor thesis deals with the problematics of a low-potential heat from the energetics point of view. It also deals with the sources and subsequent use of the low-potential heat. First of all, the thesis mentions the origin of these sources and also describes where they can be found. Also the technologies, that are able to utilize this heat, like heat pumps and solar collectors, are mentioned in this part of the thesis. In its next part, the bachelor thesis deals with use of the waste heat for heating and production of electrical energy. In the last part, the thesis presents the project that introduces a specific concept of the system for object heating from the waste heat.
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Energy supply of the complex Technicka 2
Buráň, Petr ; Lisý, Martin (referee) ; Pospíšil, Jiří (advisor)
The theme of this master thesis is „Energy supply to the site Technická 2“. This thesis is divided into several parts. First we will get in touch with concept of combined heat and power and also with principle of photovoltaic electricity production. We will continue with description of site before and after reconstruction. Then we will follow with analyze the effect of reconstruction on energy consumption. In the end we will make some suggestions for using the local source of heat and electricity in Faculty of mechanical engineering, Brno university of technology. All suggestions will be analyzed from energy and economic point of view.
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Design of industrial boiler with cogeneration
Mička, Radek ; Tvarůžek, Martin (referee) ; Křenek, Ladislav (advisor)
The diploma thesis deals with the design of the industrial boiler for biomass, Which deals with the issue of the energy future of combustion of fuels using current power generation - microcogeneration, designed for larger houses or smaller com- panies. The shape of the device is the interconnection of individual functional and technological parts of the boiler, a view of a new product that re ects its function. It uses modern and timeless materials, color and control technology to achieve overall comfort and time savings and service.
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Creation of laboratory tasks for Selected topics from renewable energy sources and energy storage
Vaněček, Lukáš ; Vaněk, Jiří (referee) ; Bača, Petr (advisor)
This master‘s thesis contains a comprehensive laboratory task about saving a heat energy with a use of changing a state of matter. The thesis is written for the object Chosen passages of renewable resources and saving a heat energy. Part one presents a theoretical part of the topic, terminology and relations needed to make this laboratory work. It also contains a created procedure of partial tasks necessary to take laboratory measurements. The second part of the thesis is about measuring and evaluation of acquired values according to procedure described in the theoretical part of the thesis. Different variations considered during the creation of measuring procedure are also named here. The final part of the thesis describes a laboratory equipment necessary to complete the task and an example of filled protocol is attached.
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Temperature as Source Sphere of Conceptual Metaphors
Hýsková, Tereza ; Vaňková, Irena (advisor) ; Pacovská, Jasňa (referee)
This thesis is focused on a research of the source domain of temperature and related concepts. It is based on the approaches of American and Polish Cognitive Linguistics. The first part is regarded to the relationship between the concepts of WARMTH / COLD and other basic concepts. The crucial part of the thesis deals with temperature as the source domain in conceptualization of emotions, emotional states and human behaviour. This part includes the description of general phenomenon in the process of mapping from the source domain of temperature to the target domain of emotions. The next chapters are focused on the Czech expressions related to separate emotions which are viewed as the target domains. There are marked the source domains of WARMTH, COLD, HEAT and FIRE in the thesis. These source domains are considered to have different roles and the human corporealness is assumed to be the main reason for the differences.
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Impact of temperature and drought on gliadins and glutenins contents in four varieties of wheat
Tomasz, Teresa ; Vítová, Eva (referee) ; Hrstka, Miroslav (advisor)
This diploma thesis deals with an influence of high temperature and water shortage on the protein content of gliadin and glutenin fractions in four varieties of winter wheat: Bohemia, Tobak, Pannonia and var. Syria with designation S46 (IG142780). The crop was grown at 26, 29, 32, 35, 38 and 41 °C during anthesis under control irrigation treatment (with soil moisture higher than 70 %) or under drought stress (with soil moisture lower than 30 %). To separate gliadins, the A-PAGE method was used, and glutenins were separated by SDS-PAGE method. Proteins were quantified by computer densitometry. Significant influence of genotype on the gluten proteins was found. Variety Pannonia has high content of -, 5-gliadins, LMW and HMW glutenins, but low content of other gliadin fractions. It was the opposite in the other varieties. Due to temperature, as well as drought, there was an increase in the content of all gluten fractions, especially of HMW glutenins, 1,2-gliadins and total gliadins. The largest increase in the gluten fractions due to drought was observed in Syria variety. In other varieties simultaneous exposure to drought and heat caused decrease in gliadin content, but increase in glutenin content. Drought at high temperatures reduced gliadin-to-glutenin ratio, mostly in Bohemia variety. This ratio has increased due to the temperature, especially in Tobak variety. For Syria variety, no effect of stress conditions was found on gliadin-to-glutenin ratio.
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