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Monitoring imidazolů v potravinách
Le Qui, Khanh Linh
This bachelor thesis deals with the formation, occurrence, and analysis of imidazoles in food. It is mainly devoted to caramel colors and imidazoles contained within. It also describes caramel as a colorant including the process of caramelization and further division of these colors into classes and their usage. Introduces the previously described effect of these substances on human health. The fact that some imidazoles have carcinogenic properties is almost confirmed. In conclusion, there is a prediction done, which way will fact above develop the technology of food production. Also briefly mentions that imidazoles also arise when cooking meat is done at home, this is not just a problem of industrial production. Briefly I describe some types of foods that contain imidazoles. One part is directly deals with the existing methods of analysis.
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Assassement of the Active Ingredient Delta-9 THC in Biological Marerial and Hemp Drugs
VONDRÁŠKOVÁ, Eliška
This bachelor's thesis deals with the matter of abusing hemp drugs and its products in the past as well as at present day. The thesis focuses on possible contributions to well-being of using hemp but also health risks, which come with the abuse of hemp and hemp drugs. The aim of this thesis is to partly chart the data about THC abuse from three South Bohemian hospitals. The hospitals are: the hospital of České Budějovice, the hospital of Písek and the hospital of Strakonice. The data involve the number of tests done in hospital facilities in order to confirm or to confute the presence of THC in biological material, most commonly in urine. The thesis describes preanalytical and analytical phase of sample treating where mistakes must be avoided. The bachelor's thesis is than occupied with the principles of the assessment of THC in biological material in both qualitative and quantitative ways of assessment of active ingredients. It contains findings gained during practice in a form of personal assistance and data from which is then made the conclusion of this thesis. The aim of this bachelor's thesis also involves charting of hemp abuse in a part of South Bohemian Region during past few years. This bachelor's thesis can serve as a base for those, who are interested in the discussed topic. Another contribution can be an extension of general knowledge amongst students and public, considering the curative benefits and the effect of hemp abuse at the same time. In the conclusion the bachelor's thesis concerns with how many tests were positive and how many were negative during the years charted in a certain hospital but also compares the hospitals mutually. It involves the development of hemp drug abuse through time in each region.
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Příprava mutantního serpinu z klíštěte \kur{Ixodes ricinus}
EDEROVÁ, Monika
Point mutation altering arginin for tryptophan amino acid residue in P1 site of tick salivary serpin Iripin-1 was created using specific primers. Recombinant protein with this mutation in nucleotide sequence was then expressed in chemically competent Escherichia coli cells, extracted from them and purified by affinity and size-exclusion chromatography. To see the impact of the mutation on inhibitory function of Iripin-1, its ability to bind trypsin and form covalent complexes was evaluated.
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Determination of glycohemoglobin by HPLC method
KUNÍKOVÁ, Martina
This bachelor thesis deals with the determination of the glycated hemoglobin by the HPLC method at two analyzers D-10 from the company Bio-Rad. The sample values for the internal control quality were measured and evaluated for two devices in the period from November 2019 to March 2020 in this bachelor thesis. The regular verification of the method was realized for both analyzers. The SEKK samples were measured in the planned survey of external quality assesment SEKK Compensation of diabetes KD 1/20. The regular comparation of both analyzers was performed by the measurement of laboratory samples once in the month. Overall 50 samples were measured in this period. The results of this bachelor thesis are part of a monitoring of the laboratory determination quality control and they are the base for the improving of the work quality in the laboratory and the base for the increase of the effectiveness of the laboratory's quality management system. Key words
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Determination of methanol derivatives in drying oils
Horká, Hana ; Hraníček, Jakub (advisor) ; Janoš, Pavel (referee)
The formation of metal carboxylates is a major problem for historical and contemporary paintings, and therefore understanding the mechanism of their formation is a crucial step for developing appropriate conservation strategies and preserving the world's cultural heritage. The determination of reactive components in drying oils is an important step towards understanding the reaction mechanism. This work is aimed at developing a simple and rapid methodology for HPLC-DAD determination of low molecular weight degradation products of traditional drying oils, namely methanol, formaldehyde and formic acid. In this work, QuEChERS conditions for the extraction of methanol, formaldehyde and formic acid from various oil samples (olive, walnut, poppy and linseed oils) were optimized followed by purification of the samples from the oil matrix using a commercial d-SPE sorbent (Z-Sep/C18) and two nanostructured sorbents, CeO2 and ZrO2.
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Determination of non-esterified fatty acids in plasma
Lacinová, Petra ; Pacáková, Věra (advisor) ; Staňková, Barbora (referee)
The determination of non-esterified fatty acids (NEFA) in plasma is possible by many routine biochemical methods. For more detailed metabolic studies it is required to analyse the fatty acid (FA) profile. The NEFA profile determination is usually performed by the capillary gas chromatography (GC), but the sample preparation is not uniform. NEFA may be isolated by the extraction process or by the preparative thin-layer chromatography (TLC). The aim of this study was to compare these two separation procedures. The samples of pooled plasma from volunteer donors were analyzed by capillary GC after previous separation procedure by the TLC and the liquid-liquid extraction. The results were compared by the t-test for both the absolute concentration of individual FA provided by the internal standard (margaric acid) method and the relative abundance (Rel%). The reproducibility of the results was significantly better for the liquid-liquid extraction method than the TLC. The relative standard deviations (RSD) of the FA groups (unsaturated, monosaturated and polysaturated) were from 4.3 to 11.3 % vs. 8.6 to 33.8 % for the relative abundance and from 16.5 to 25.5% vs. 15.4 to 47.4% for the absolute concentration. For each FA, which were represented by more than 0.1 Rel %, RSD ranged from 4.5 to 47.8% vs. 9.5 to...
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Optimization of microextraction technique for analysis of water by chromatographic methods
Otrubová, Lucie ; Čabala, Radomír (advisor) ; Sobotníková, Jana (referee)
New liquid phase microextraction (LPME) has been optimized. Plastic adapter with tapered tip was used as extraction device. Concentration of analytes was determined by GC-MS. Face centered design was used to optimize LPME and the results were evaluated by Minitab 16 programme. Volume of sample, volume of extraction solvent, stirring rate, extraction time, ionic strength and time after microextraction were optimized. Response of system was defined as the sum of the peak relative areas. Toluene, tetrachlorethylene, ethylbenzene, xylenes, mesitylene and naphthalene were the studied analytes. Methylhexadecanoate was used as an internal standard. Optimal system conditions were as follows: 20 mL sample volume, 300 μL extraction solvent volume, 20 minutes, stirring speed 700 rpm, no addition of salt, and time after microextraction 3.5 minutes. Determination coefficient was 0.9700 and the lack-of-fit was insignificant which indicated good agreement of the model with the experimental data. The preconcentration factor ranged from 26 (tetrachlorethylene) to 39 (xylenes). The microextraction yield was varied from 39.5% (tetrachlorethylene) to 59.1% (p-xylene and m-xylene). The new method was tested by analyzing real samples of tap water, river water and water from the sewage treatment plant. The samples were...
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Methods Activating Students in Education of Chemistry
Kesnerová Řádková, Olga ; Bílek, Martin (advisor) ; Klečková, Marta (referee) ; Dobrovská, Dana (referee)
Topic: Methods of Activating Students in Education of Chemistry Author: Olga Kesnerová Řádková Abstract This dissertation work aims at teaching methods in the subject of chemistry and it concentrates mainly on methods with quite a high proportion of activation of pupils. The benefits and disadvantages of activating teaching methods, possible criteria for their choice, conditions for their putting into practice and similar, are described. Later, the work focuses on four chosen activating methods, namely brainstorming methods, project method, didactic games and work with a fictional and another type of a text which is applied here into natural science teaching, namely into chemistry teaching. A story with a chemical experiment can serve as an example. The research part contains a case study of putting project teaching methods into practice which includes a record and analysis of the condition and of the problem connected with its implementation, as well as the initiation of the project with the help of brainstorming methods. Participation observing, questionnaires, mini polls and discussions were used in our case study. Altogether 184 second year students for the teaching profession of the Pedagogical Faculty of Hradec Králové University took part in the research. On the basis of the results of the research...
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