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Study of the relations between the structure and function of C - terminal vanilloid receptor TRPV1
Gryčová, Lenka ; Obšil, Tomáš (advisor) ; Heřman, Petr (referee) ; Urbánková, Eva (referee) ; Krůšek, Jan (referee)
Transient receptor potential channel vanilloid receptor subunit 1 (TRPV1) is a thermosensitive cation channel activated by noxious heat as well as a wide range of chemical stimuli. Although ATP by itself does not directly activate TRPV1, it was shown that intracellular ATP increases its activity by directly interacting with the Walker A motif residing on the C-terminus of TRPV1. In order to identify the amino acid residues that are essential for the binding of ATP to the TRPV1 channel, we performed the following point mutations of the Walker A motif: P732A, D733A, G734A, K735A, D736A, and D737A. Employing bulk fluorescence measurements, namely a TNP-ATP competition assay and FITC labeling and quenching experiments, we identified the key role of the K735 residue in the binding of the nucleotide. Experimental data was interpreted according to our molecular modelling simulations. Calmodulin (CaM) is known to play an important role in the regulation of TRP channels activity. Although it has been reported that CaM binds to the Cterminus of TRPV1 (TRPV1-CT), no classic CaM-binding motif was found in this region. In this work, we explored this unusual TRPV1 CaM-binding motif in detail and found that five residues from a putative CaM-binding motif are important for TRPV1-CT's binding to CaM, with arginine R785...
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The History of the Sokol Movement in Lysé nad Labem
Herman, Petr ; Kašpar, Ladislav (advisor) ; Zachariášová, Jana (referee)
Sokolská organizace má za sebou bohatou minulost a je navždy zapsána do historie tohoto státu. Z jejích řad vyšlo mnoho obránců naší republiky, ať už jako legionáři nebo bojovníci za svobodu na bojištích druhé světové války. Sokol z nich vychoval lidi s vynikající morálkou i fyzickou kondicí. Lyský Sokol vznikl jen pár let po "průkopnickém" Sokole v Praze a hned byl velký zájem o členství v jednotě. Bohužel tragickou nehodou ve městě, která vyvolala krizi, byla činnost jednoty záhy na 2 desetiletí neúmyslně pozastavena. Naštěstí obyvatelé nezapomněli a Sokol znovu ožil a dále sílil. Tentokrát organizaci do cesty vstoupila válka, a tak činnost ochabla. Po válce se vzchopil a zažíval svá nej lepší léta. S druhou světovou válkou byla činnost Sokola opět zastavena a tentokrát ani po skončení neměl příliš prostoru k rozletu, komunistická diktatura ho též shledala nepřípustným. Přesto se Sokolové nevzdali a přes mnoho nepravostí působil v ilegalitě. Revoluční rok 1989 mu opět otevřel brány. Tak jak byl Sokol v rozkvětu za první republiky, si však dnes můžeme nechat zdát, ačkoliv dle mého názoru jsou jeho myšlenky stále aktuální. Bohužel dnešní společnost, jak se zdá, upřednostňuje zřejmě jiné cíle a hodnoty.
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Robot Localization Using Camera
Heřman, Petr ; Španěl, Michal (referee) ; Beran, Vítězslav (advisor)
The objective of this work is to design a simple localization method and its implementation in robot operating system ROS. This method uses a monocular camera as the only sensor and estimates the position in a known map. In experiments with prototypes are tested key points of type SURF, SIFT and ORB.
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Robot Controlled by PIC Microprocessor Unit
Heřman, Petr ; Luža, Radim (referee) ; Rozman, Jaroslav (advisor)
This thesis describes design of a cheap robot. It includes implementation of firmware of low level control unit based on microcontroller PIC. The firmware drives motors, gains sensors data and communicates with the high level control unit. Furthermore the thesis presents realisation of connection to the robotic operation system ROS and its standard structures allowing usage of existing packages for the robot teleoperation and displaying sensor data on the remote computer. The thesis finally reports experiments with the robot. The constructed prototype is the model of the robotic lawn mower, however the whole solution has universal usage.
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The Protection of Consumer in Travel Insurance
Rasochová, Jitka ; Horová, Olga (advisor) ; Herman, Petr (referee)
This Bachelor's Thesis deals deals with protection of consumer within the general protection of interested party in travel insurance, policy holders or insured person, in relation to their position in relationship with the insurance company. The work is divided into theoretical and practical part. The theoretical part explains the legislation protecting consumer rights, basic principles of insurance and description of the travel insurance. The aim of this Bachelor'sThesis is to analyze, wheter the legal requirements on the czech insurance market are able to protect the consumer. The practical part deals with the most frequent potential risks in insurance protection when traveling abroad by identifying problem areas, particularly controling the quality of distribution, providing information on insurance broker and clarity of policy conditions and dealing with complaints.
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