|
|
Artephyletics in group work in free time
Semecká, Hana ; Hanková, Zdeňka (advisor) ; Blažková, Veronika (referee)
This bachelor thesis examines the topic of artephiletics in use during leisure activities, aimed at children's perception of the natural environment. The source of perception is trees, which provide inspiration for spontaneous and organised creative activities. Children's insights and perceptions into the world of trees create space for shared experiences, knowledge, stimulate emotions and lasting stimuli. In the theoretical part, the first chapters introduce the concepts of education, leisure time, and pedagogy of leisure time as used within the pedagogical field of leisure. The chapter on art describes the relationship between nature and education as an educational tool. The chapters on children's drawing and art education define the activities in the context of the Framework Curriculum in school and during leisure time on the basis of J. Slavik's concept of art education. The theoretical part concludes with a chapter on artephiletics with an emphasis on the importance of pedagogical work. The aim of my bachelor's thesis is to design and test the Tree Stories artephiletic leisure project. The project focuses on children's perception of trees by experiencing them during art activities. The practical part characterises a group of children with whom the project was carried out. It describes the activities...
|
|
|
Spectres and Illusions
Arendárik, Ján ; Stratil, Václav (referee) ; Artamonov, Vasil (advisor)
The subject of my practical diploma thesis is a painting reaction to the photographs from years 1915 to 1930. I see them as a " photographs of dead(lost) world. The photographs of people whose dont live in present but the photographs are evidence of their existence in the past.
|
|
|
Nucleotide density for finding CpG islands
Sikorová, Eva ; Provazník, Ivo (referee) ; Maděránková, Denisa (advisor)
This bachelor thesis deals with searching for CpG islands in the DNA sequences using the nucleotide density vectors. The first part includes view of the DNA structure, the genetic information expression, more detailed analysis of CpG islands and primarily their detection methods. In the practical part the algorithm for graphical representation of nucleotides on the basis of their densities and detections of CpG islands of artificially created and real DNA sequences was realized in the MATLAB program. The thesis includes the analysis of twenty sequences with the expected content of CpGIs and the comparison of results between the created program and two search engines.
|
|
|
Vliv expozice těžkých kovů na expresi mRNA isoforem metalothioneinu u adherentních buněčných linií
Jordanová, Lucie
Metallothionein is a small cysteine-rich protein that plays an important role in metal homeostasis and protection against heavy metal toxicity, DNA damage and oxidative stress. This bachelor thesis was conducted to investigate the effect of heavy metal exposure on the mRNA expression of metallothionein isoforms in adherent cell lines. The effect of zinc exposure in the form of ZnSO4 on the mRNA expression of MT-1A, MT-1X and MT-2A isoforms in adherent breast cancer cell lines MCF-7, MDA-MB-231 and T-47D was investigated. Using MTT viability assay, the cell viability changes at different ZnSO4 concentrations and RNA integrity is verified using denaturing agarose gel. Polymerase chain reaction combined with reverse transcription qRT-PCR is used to study mRNA levels.
|
|
| |
|
| |
|
|
Art group 12/15 and its place in the second half of the 20th century Czech art
Mayerová, Miroslava ; Bláha, Jaroslav (advisor) ; Kornatovský, Jiří (referee)
Miroslava Mayerová Title of the Bachelor's Thesis: Art group 12/15 and its place in the second half of the 20th century Czech art Abstract This thesis focuses on the evolution and influence of art group 12/15 on socio-cultural development of society in the second half of the 20th century. The theoretical part encompasses the socio-cultural environment and historical situation before and during the formation of the group, describes its formation, and presents the work of individual group members until their second joint exhibition. The didactic part teaches students the group's activities by utilising concepts used by 12/15. The practical part of the work carries out didactic task and by form of self-assess evaluates its feasibility and usefulness. The work includes historical documents and articles about group.
|
|
| |
|
|
Aesthetic Factor in John Dewey's Conception of an Experience Aesthetic Relevance demonstrated by Example of Snowbording
Vyhnánková, Klára ; Kaplický, Martin (advisor) ; Dadejík, Ondřej (referee)
This diploma thesis examines John Dewey's concept of experience, focusing especially on his notion that an aesthetic factor is present in every complete and consummatory experience. It deals with the differences between a common experience and "an experience" and examines how an aesthetic factor works in them. The indispensable role of the aesthetic factor is intentionally demonstrated on the non-artistic example of experiences of snowboarding. The most important findings are developed through the approach of Richard Shusterman's somaesthetics. The theories of these two philosophers support the main idea of this thesis, namely that dynamic movements of the body can bring aesthetic experiences to their actor. Thus, some of the reasons that explain the attractiveness of this modern sport come to light that are not apparent at first glance.
|
|
|
Preparation of the 14-3-3 Protein Binding Partners for Structural Studies.
Kopecká, Miroslava ; Obšil, Tomáš (advisor) ; Teisinger, Jan (referee)
Tyrosine hydroxylase belongs to the group of hydroxylases of aromatic acids and catalyzes a key step in the biosynthesis of catecholamine neurotransmitters. The tyrosine hydroxylase possesses the homotetrameric structure and contains three structural domains: the N-terminal regulatory domain, the catalytic domain and the C-terminal tetramerization domain. The activity of tyrosine hydroxylase is regulated by phosphorylation and through the regulation of its expression. Phosphorylation at Ser-19 induces binding of the 14-3-3 protein, which affects the structure of the regulatory domain and protects it against both dephosphorylation and degradation. Since the structure of the regulatory domain is still unknown, we decided to perform its structural characterization using NMR techniques. First, the expression and purification protocol of the regulatory domain of tyrosine hydroxylase was optimized. The protein was expressed as a His-tag fusion protein and its purification is composed from two steps: the chelating chromatography and the size-exclusion chromatography. The dynamic light scattering and the 1 H nuclear magnetic resonance were used to verify its monodispersity, and hence its suitability for further experiments.
|
guest ::
