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Auxin Transport in Arabidopsis thaliana: From the whole plant to suspension cultured cells
Seifertová, Daniela ; Zažímalová, Eva (advisor) ; Opatrný, Zdeněk (referee) ; Hejátko, Jan (referee)
in English Plants with their sessile life-style are exposed to many stimuli from environment. They have developed mechanisms how to coordinate their growth and development, which allows them to survive sometimes in very difficult conditions. Plant hormones are one of the most important regulators of this signal transduction. Auxins, as the oldest known group of plant hormones, play important role in many physiological processes in plants. To allow perceiving the information in every single cell, auxin molecule is transported by cell-to-cell manner. Auxin molecules enter the cell by passive diffusion or by active uptake by auxin influx carriers. To reach the next cell, they are transported actively out of the cell by auxin efflux carriers. Athough active auxin transport has been studied for almost four decades, past two decades contributed to the identification and characterization of particular auxin carriers remarkably. This thesis contributes to the knowledge on the auxin efflux and influx carriers and their involvement in the processes occurring from a single cell level to the level of a whole plant. Firstly, it brings detailed description of auxin transport characteristics in Arabidopsis thaliana cell suspension cells (Ath cells). Secondly, it shows that the overproduction of PIN1 auxin efflux...
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Fundamental Analysis of Selected Stocks
Stejskalová, Lenka ; Procházka, Roman (referee) ; Sojka, Zdeněk (advisor)
Diploma thesis deals with the determination of the intrinsic value of Philip Morris ČR a.s. shares using methods of fundamental stock analysis. The theoretical part is focused on clarifying the basic concepts and methods that relate to this issue and will be used in the processing of the following parts. The analytical part contains fundamental stock analysis compiled on macroeconomic, branch and corporate level. The proposal part focuses on the application of selected methods to quantify the intrinsic value of shares, including the subsequent recommendation for investors.
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The measurement of total alkaloids in tobacco
Pipková, Renata ; Vránová, Dana (referee) ; Omelková, Jiřina (advisor)
The aim of this diploma thesis is the assay of total alkaloids in different tobacco types. To measure the content of total alkaloids are utilized types of tobacco used for preparation of tobacco blends and consecutive production of cigarettes. The samples of analyzed tobacco include various countries of cultivation and different crop years. There is described tobacco as a plant, in it contained alkaloids and the utilization of tobacco in the theoretical part. In more detail it deals with the production and construction of cigarettes which is closely related to the content monitoring of total alkaloids in tobacco. The contents of total alkaloids are determined by the continuous flow analyzer- SKALAR. On this spectrophotometer are set the contens of reducing sugars as well. The comparison of total alkaloids contents and reducing sugars from the perspective of single tobacco types, countries of cultivation and crop years is clearly summarized in the experimental part of the thesis that was performed in the company Philip Morris ČR, a.s.
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Electronic cigarettes and public view
Škaryd, Jan ; Zmrzlá, Petra (referee) ; Mihai, Hana (advisor)
This work is focused on both smokers of electronic and conventional cigarettes. The purpose of this work is to introduce and describe an electronic cigarette, its parts and compare its harmfulness with conventional cigarettes, especially in the current situation when smoking is not so fashionable in public. Based on the findings, it is noticeable that electronic cigarettes are trying to consolidate their position on the market on the one hand for a relatively short time, but on the other, successfully.
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Význam rostlinných proteinů z podrodiny ABCB pro transport auxinu
Kubeš, Martin ; Zažímalová, Eva (advisor) ; Hejátko, Jan (referee) ; Reinöhl, Vilém (referee)
Polar auxin transport provides essential directional and positional information for many developmental processes in plants. At the cellular level, it is realized by both passive diffusion and the active transport through the membrane proteins - AUX1/LAXes, PINs and ABCBs. The aim of this thesis was to characterize the role of ABCB1, ABCB4 and ABCB19 proteins in polar auxin transport using transformed tobacco BY-2 cell lines. It was shown that the plasma membrane (PM) localization of the ABCB1, 4 and 19 is not polar. The ABCB4 was also more stable on PM after the treatment with auxin influx inhibitors; making use of ABCB4-cell line helped to uncover new characteristics of markers of endocytosis - the FM- dyes. The induction of ABCB19 has led to a decrease in 3 H-NAA accumulation with characteristic auxin starvation phenotype, similar to PIN7 overexpressing cell line, that could be rescued in case of PIN7 cell line by application of the auxin efflux inhibitor NPA; however, the accumulation of auxin in ABCB19-overexpressing cell line was less sensitive to NPA and the rescue of the auxin starvation phenotype was ineffective. Importantly, unique property of the ABCB4 was demonstrated: It displayed dual, auxin-concentration-dependent auxin transport activity in Arabidopsis roots, tobacco BY-2 and yeast cells. The...
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Patterns of heated tobacco products usage among women: qualitative research
Prokešová, Miroslava ; Kulhánek, Adam (advisor) ; Kočvarová, Linda (referee)
Introduction: A relatively new trend that has been gaining popularity in recent years is heated tobacco, which has been on the Czech market since 2017. Although the number of users of heated tobacco products in the Czech Republic is increasing, the phenomenon is still relatively under-mapped. There is also very little information on the gender specificities of the use of heated tobacco. Therefore, this study focuses on mapping and describing the patterns of use of heated tobacco products among women who have switched from using conventional cigarettes to heated tobacco products. Aims: The main aim of the research is to map the patterns of use of heated tobacco products among chosen women in the adult population of tobacco users in the Czech Republic. The sub-objectives are to describe the nature of heated tobacco product, find out what motivated female users to switch to heated tobacco products. And what are the typical situations when women use the device. Sample: the research sample was selected by simple intentional (purposive) sampling method, combined with snowball sampling method. The final sample consisted of 7 female respondents. The mean age was 25,7 years (youngest 21 years and oldest 34 years). Methods: The research was conducted using a qualitative method. Data was collected by...
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The impact of advertisement and promotion of alternative tobacco and nicotine products on tobacco users: online survey
Jirásková, Alžběta ; Kulhánek, Adam (advisor) ; Zachová, Kateřina (referee)
Background: In the last few years, a large number of nicotine products have appeared on the Czech market, which are intended, among other things, to serve as alternatives to conventional cigarettes. These alternatives are often visually very distinctive and attractive. They are often perceived by users as less harmful and suitable for substitution for conventional cigarettes. At the same time, these products are not subject to legislation as strict as conventional cigarettes or packaged tobacco. Currently, approximately one quarter of the Czech population are at least occasional smokers, and a significant proportion are either currently using alternative nicotine products or are considering changing their use. Aims: The main objective of this work was to determine how active tobacco users perceive new alternatives on the market, specifically in terms of health harms, marketing presentation and appearance. In particular, another objective is to map preferences in the choice of use form and to compare the above mentioned alternatives with conventional cigarettes. Methods: The data was obtained through quantitative research. It was carried out using an online questionnaire survey in the Survio platform interface and distributed in the social networking environment. Respondents were collected through...
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Tobacco treatment needs analysis among clients and their priority of tobacco cessation intervention in Addiction Treatement Department at Day Care Centre Ondřejov: a questionnaire survey
Hrabovská, Adéla ; Kulhánek, Adam (advisor) ; Svěcená, Kateřina (referee)
Univerzita Karlova v Praze 1. lékařská fakulta Studijní program: Specializace ve zdravotnictví Studijní obor: Adiktologie Bc. Adéla Hrabovská Abstrakt v anglickém jazyce k diplomové práci Mapování potřeb klientů v oblasti léčby závislosti na tabáku na Oddělení léčby závislostí Denního psychoterapeutického sanatoria Ondřejov: dotazníkový výzkum Vedoucí práce: Mgr. Adam Kulhánek, Ph.D., MBA PRAHA 2023 ABSTRACT INTRODUCTION - The prevalence of the use of tobacco and nicotine products is higher in the clinical population of dependence treatment services than in the general population, Interventions for tobacco and nicotine use cessation are hardly ever available for the patients. AIM - The aim of the research is to map the needs of the clients of the Addiction Treatment Department in the Day care Psychotherapeutic Sanatorium Ondřejov (hereinafter "DPS Ondřejov") in the area of their tobacco and nicotine use disorder and its treatment. METHODS - This research is an evaluation study, specifically an analysis of the needs among the clients in DPS Ondřejov. It is a questionnaire survey. Data were obtained using a questionnaire consisting of a non-standardized part and the Fagerström Nicotine Dependence Test. The data were transcribed from the printed questionnaire into electronic form. The descriptive statistic...
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The decrease in alcohol consumption and smoking in the Czech Republic between the years 2010 and 2018 in relation to the family environment
Krásná, Anna ; Soukup, Petr (advisor) ; Petrúšek, Ivan (referee)
Since 2010, sociologists have noted that teenagers drink less alcohol and smoke a smaller number of cigarettes each year than in previous years. This paper analyses the association between family background and the decline in alcohol and cigarette use in the Czech Republic between 2010 and 2018. The data are from the international HBSC (Health Behaviour in School-aged Children) study, from the years 2010, 2014, and 2018. The bachelor thesis only discusses cases from the Czech Republic, the data have thousands of cases, so they are robust enough to perform quantitative analyses. The analysis focuses only on alcohol and conventional cigarettes as the two most common addictive substances consumed by adolescents. Family background is examined in terms of parental presence in the household, ease of communication between parent and child, and family wealth. Secondary analysis showed an association of family wealth with the amount of alcohol consumed and cigarettes smoked. Children from wealthier families drink alcohol more often than children from the opposite end of the spectrum, and conversely, children from poorer families smoke more cigarettes. The data does not have the potential to observe causality, only correlations could be observed. The main conclusion of the paper is that the decline in...
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Vybrané sociálně patologické jevy na střední škole
Dudová, Jana
This bachelor thesis deals with the socially pathological phenomena in selected high schools in relation to a given region. Its theoretical part briefly specifies the basic issues of the socially pathological phenomena and the drug addiction among adolescents with a detailed focus on the use of tobacco products, including effects, consequences, causes and preventive measures. The main methods of the theoretical part, namely, study and use of the available literature sources allow to obtain relevant data for characteristic of the observed problem in the high schools environment. The goal of the practical part lies in a realization of a questionnaire survey which is applied to students of the selected high schools in the particular locality. The survey effort is to determine a present state of the tobacco use and a degree of appearance of the eventual addiction to the consumption of that type of the drug. Detailed analysis, confrontation of the results with respect to a gender of the pupils and the high school environment including the processing of ascertained materials into the transparent tables and graphs are seemed to be as a self-evident task. All of that will be utilized for the final summary, discussion and conclusion.
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