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Virtual laboratory for simulation of biological processes on membranes
Beneš, Martin ; Bílý, Tomáš (advisor) ; Hoksza, David (referee)
The purpose of this thesis is the creation of a virtual computer laboratory, where it will be possible to simulate and analyze the behavior on the biological cell membrane. The membrane and its surroundings are described as a hex-net with other chemicals and components that interact with each other. Depending on this distribution, it is possible to observe well-known membrane phenomena such as the amplification of the action potential. The simulation itself should show, that for the basic membrane-activity modeling, you only need a few basic laws of physics and chemistry.
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Financing of Business Activities
Beneš, Martin ; Fojtů, Kateřina (referee) ; Ptáček, Roman (advisor)
The bachelor’s thesis deals with appropriate choice of financing business activities. In my work, I focusing on financing a car in the company and I have different options of financing. The first part concentrates on theoretical division and description of individual possibilities of financing. The second part analyze the best of the chosen possibilities of financing.
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Determination of dissociation constants and limiting mobilities of some ampholytes
Maliňáková, Lucie ; Riesová, Martina (advisor) ; Beneš, Martin (referee)
Capillary zone electrophoresis is a simple and automated method which enables simultaneous determination of the dissociation constants and limiting ionic mobilities of the analyte from one series of experiments. The small consumption of the sample is main advantage of this method. The dependence of the effective mobilities of 7 selected ampholytes on pH in the range of 1.6 to 6.8 was measured. A set of simple buffers with constant ionic strength and satisfactory buffering capacity was designed using computer program PeakMaster. The ampholytes were detected by contactless conductivity detector. Dynamic capillary coating was used to minimize interactions of analytes with silanol groups on the inner wall of the capillary. Experimentally obtained effective mobilities were fitted with the suitable function. Actual mobilities and the mixed dissociation constants corresponding to the actual ionic strength were parameters of the regression function. Thermodynamic dissociation constants and limiting ionic mobility of ampholytes were calculated using the extended Debye-Hückel law with the linear member and the Onsager law. Key words: capillary zone electrophoresis, dissociation constant, limiting mobility, ampholytes
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Signal transduction in olfactory sensory neurons of vertebrates and tools for the computer simulation
Beneš, Martin ; Zápotocký, Martin (advisor) ; Hejnová, Lucie (referee)
The purpose of this thesis is summing up the information about olfactory transduction of vertebrates. This review is divided into four parts, each part focuses on a different aspect of olfactory transduction. First there is an overview of basic electrophysiological methods used for transduction research, followed by a description of a complete transduction on a molecular level. Next is a summary of model types and their use in olfactory transduction simulation, including a detailed description of two models: One of them describes the beginning of olfactory transduction, from the odorant binding on the receptor to the cAMP production, the other deals with the negative feedback of Ca2+. Finally there is an overview of software products designed to create and analyze the models from the preceding section.
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Personality and work of Karel Richter,author of the non-fiction literature in the medial and socio-political metamorphoses of time
Beneš, Martin ; Halada, Jan (advisor) ; Čeňková, Jana (referee)
The Thesis entitled "The Personality and Work of the Author of the Non fiction literature in the Media and the Socio-Political Metamorphoses of the Time" describes the influence of the various aspects of the communication process and its development (changes of the socio-political situation and the non fiction literature itself) in relation to the publicist, writer, historian and translator Karel Richter. He lived through the 1960s, the period of "normalisation" and the 1990s, when the market economy witnessed a rapid development - including the publishing sector. The work describes the metamorphosis of the communication process in retrieving information, the pursuit of political communication in relation to the non fiction literature and other aspects of this genre of the author's works. It focuses on the political and economic influences, which affected Richter's work, and the influence of his work in the media back on him. The Thesis is divided into a theoretical and a practical part. The theoretical part sums up scientific works written about the non fiction literature genre. In his own analyses Richter, too, tries to characterize the genre and its basic problem, which rests in non-established and diverse definitions often contradicting each other. This chapter also sums up the development of...
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Determination of stability constants of charged cyclodextrine complexes by capillary electrophoresis
Beneš, Martin ; Dubský, Pavel (referee) ; Zusková, Iva (advisor)
Martin Beneš Determination of stability constants of charged cyclodextrin comlexes by capillary electrophoresis Abstract Stability constant characterizes binding interaction between an analyte and complexation agent. These interactions play very important role in separation processes of, in other way undistinguishable, compounds, e.g. enantiomers. The most widely used complexation agents are cyclodextrins. Affinity capillary electrophoresis (ACE) belongs to methods suitable for the determination of stability constants. The stability constant is determined from the dependence of the effective mobility of analyte on the increasing concentration of complexation agent in background electrolyte (BGE). If charged CDs are used, the attention must be paid not only to viscosity of the BGE and to the influence of Joule heating on the temperature in the capillary but also to the increasing ionic strength. The thermodynamic stability constants of R,R- and S,S-hydrobenzoin and R- and S-(3-brom- 2-methyl-1-propanol) with cationic modified β-cyclodextrin: 6-monodeoxy-6-mono(3- hydroxy)propylamino-β-cyclodextrin hydrochlorid (PABCD) were determined by affinity capillary electrophoresis. The average temperature (25řC) of the BGE in the capillary was kept constant. This was achieved by decreasing of the cassette temperature...
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Epistemologic limits of selected approaches to language
Beneš, Martin ; Lehečková, Eva (referee) ; Macurová, Alena (advisor)
The aim of this text is to comment and critically evaluate the epistemology of selected approaches to language: namely European structuralism (Saussure, Hjelmslev, Prague School), generative grammar, the linguistics of la parole and text linguistics and cognitive approaches to language. In particular, the aim is to comment epistemological limits of these approaches with regards to the two basic facts. Firstly, with regard to the fact that what these approaches define as their object field is in fact only a partial section of the whole subject field of linguistics (looked upon through the purposes of this work as a specifically structured communication model). Secondly, this text will investigate the relations between these approaches and an important claim of the philosopher of science T. S. Kuhn. Kuhn in his widely known book The Structure of Scientific Revolutions says: "There is, I think, no theory-independent way to reconstruct phrases like ‚really there'." (Kuhn, 1970, p. 206). That means whether claims based on epistemology of particular approaches describe objective reality or only give us a description of their own theoretical constructs.
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