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Supervision with Visual Art Expression
Hanzlová, Andrea ; Havrdová, Zuzana (advisor) ; Slavík, Jan (referee)
Title: Supervision with Visual Art Expression Author: Bc. Andrea Hanzlová ABSTRACT Although art expression has been widely used in both art therapy and supervision in the Czech Republic, its distinctive features, added value and possible drawbacks in supervision have been researched less extensively than in art therapy. Here I explore what art expression brings into supervision, what new perspectives it can offer and what obstacles may dissuade supervisors from employing certain approaches. A qualitative content analysis of semi- structured interviews on the sample of 12 respondents revealed four thematic areas: Definition of creative art process, phases and content; Drawbacks and Benefits of working with art expression; The role of education in used methods and approaches. The results show an unexpectedly wide range of methods that Czech supervisors and supervisees employ when dealing with art expression in supervision, as well as the extent or absence of some approaches. One possible reason could be the lack of legislative anchoring compared to countries such as the UK and USA. Key words: expression, visual creative process, artefact, expressive therapy, art therapy, supervision, supervision of art therapies
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Characteristics of expression vectors for Corynebacterium glutamicum and their use for studies of sigma factors of RNA polymerase
Dvořáková, Pavla ; Pátek, Miroslav (advisor) ; Konopásek, Ivo (referee)
The aim of the thesis was to characterize chosen expression vectors used in biotechnologically important bacterial species, Corynebacterium glutamicum, and to test their use in studies of promoter activity control by sigma factors of RNA polymerase. Different properties of these vectors (level of expression of the cloned gene, leaky expression without inducer, dependence of expression level on inducer concentration and cell population homogeneity) were found by determination of expression level of the model gfpuv gene by fluorescence intensity assay of the produced protein and by gfpuv-expressing C. glutamicum cell population analysis using flow cytometry. The vector pEC-XT99A was chosen for testing the bi-plasmid system for assignment of a sigma factor to the chosen promoter. Although the level of expression provided by pEC-XT99A was not high, the vector showed no leaky expression, expression from the vector was comparable for a wide range of IPTG concentrations and the cell population was homogenous concerning the gene expression. Using pEC-XT99A from which individual stress sig genes were expressed, the σD factor was clearly assigned to the up-to-now unknown Pcg0420 promoter. Another vector for isolation and purification of C. glutamicum proteins was used to express the C. glutamicum sigM gene and to...
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John Dewey's Aesthetics and interactive art
Rubášová, Veronika ; Kaplický, Martin (advisor) ; Dadejík, Ondřej (referee)
This bachelor thesis is especially focused on John Dewey's theory. The first and the main part of the bachelor thesis deals with Dewey's conception of experience, with analysis of this process of experience and with aesthetic level of experience. Significant part of bachelor thesis is dedicated to aesthetic experience with art as well, which is discussed in the context of every day experiences. Continuity between both is highlighted. The second part of the bachelor thesis is an addendum to the interpretation of Dewey's theory. This part of the thesis deals with interactive art. Interactive art is interpreted using Dewey's theory. Interactive art is in this paper understood as artistic type which can emphasize continuity between art and everyday experience so as Dewey does.
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The effect of selected endocrine disruptors on the cytochromes P450 1A1 and 2C
Klusoňová, Zuzana ; Bořek Dohalská, Lucie (advisor) ; Linhartová, Lucie (referee)
Many currently produced chemicals reveal specific properties which allow them to be referred to as endocrine disruptors (ED). These substances exhibit an exogenic hormone activity and usually act as antagonists or agonists of endogenic hormones. The exogenic EDs studied in this work were 17α-ethinylestradiol (EE2) and benzoapyrene (BaP). 17β-estradiol (E2), a typical endogenic hormone, was also included to the study. In the presented work, the effect of these EDs and their combinations on the expression and specific activities of cytochromes P450 (CYP) 1A1 and 2C was determined. First, the microsomal fraction (MF) of liver, kidney and lung of rats premedicated with these compounds or without premedication was isolated. CYP expression was assessed by the Western blot analyses in these MF samples. Moreover, CYP1A1 and CYP2C specific activities were evaluated. It was found that premedication of rats with BaP increased CYP1A1 expression in all above mentioned organs. Whereas BaP strongly induced rat CYP1A1, EE2 and E2 were almost without this effect. But, when these disruptors were administered to rats with BaP, they supported its potency to induce CYP1A1. Further, CYP2C11 expression and its specific activity were gently increased by premedication of rat with EE2 and its combination with BaP....
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Art group 12/15 and its place in the second half of the 20th century Czech art
Mayerová, Miroslava ; Bláha, Jaroslav (advisor) ; Kornatovský, Jiří (referee)
Miroslava Mayerová Title of the Bachelor's Thesis: Art group 12/15 and its place in the second half of the 20th century Czech art Abstract This thesis focuses on the evolution and influence of art group 12/15 on socio-cultural development of society in the second half of the 20th century. The theoretical part encompasses the socio-cultural environment and historical situation before and during the formation of the group, describes its formation, and presents the work of individual group members until their second joint exhibition. The didactic part teaches students the group's activities by utilising concepts used by 12/15. The practical part of the work carries out didactic task and by form of self-assess evaluates its feasibility and usefulness. The work includes historical documents and articles about group.
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Study of effect of flavonoids on biotransformation enzymes expression
Koucká, Kamila ; Moserová, Michaela (advisor) ; Mrízová, Iveta (referee)
Flavonoids belong to the group of phenolic compounds included in the secondary metabolites of plants, which are represented mainly in fruits and vegetables. These compounds have antioxidant and anti-carcinogenic effects. Some of them can affect biotransformation enzymes, which include the cytochrome P450 and can interfere with the metabolism of xenobiotics. In the present work we studied effect of dihydromyricetine on the gene expression of cytochrome P450 1A1, 1A2 and cytochrome b5 in livers, lungs, kidneys, colon and small intestine of laboratory rats. The small intestine was divided into three parts namely a proximal, middle and distal. At first, isolation of total RNA was made using commercial reagents and isolated RNA was converted to cDNA by reverse transcription. Finally, using polymerase chain reaction in real time (RT-PCR), the relatively gene expression of genes observed in the organs of laboratory rats pretreated dihydromyricetine and control laboratory rats (untreated) to a reference gene β-actin was determined. It was found that dihydromyricetine did not significantly affect the gene expression of studied genes in most organs. However, a significant decrease of gene expression of CYP1A1 and CYP1A2 was observed in the lungs. On the contrary, an increase of gene expression of CYP1A2 was...
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Theatre as a Window to the World of Mental Disability
Macková, Anna ; Chrz, Vladimír (advisor) ; Onder, Jakub (referee)
The thesis provides a qualitative analysis of an autobiographical play based on journals written by a mentally challenged woman. The analysis is founded on authentic personal experience, the script of the play and a video recording of its performance. The empirical section is preceded by a theoretical introduction focused on mental disability in relation to theatre. The empirical section begins with the question: What can we learn about a mentally disabled person through theatre? The analytical process consists of three levels: a content analysis of the performance, an analysis of the author's perspective and the viewpoint of an implied spectator. The analysis shows that the story contains common autobiographical topics and the author barely reflects her disability. The creative perspective consists of the process of adapting the story into a play. Theatre also plays a therapeutic role and raises awareness. There is an imaginary window on the boundary between the stage and the audience, one that the author opens from within and the viewer from without. During the course of the play, the implied spectator becomes confronted with their prejudice and an accumulation of ambivalent feelings. This process ends in a catharsis and a genuine encounter with the actor's world and her spontaneity. KEY WORDS...
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The use virus derived vectors for the expression of proteins in plants
Kratochvílová, Kateřina ; Moravec, Tomáš (advisor) ; Holá, Dana (referee)
The tobacco mosaic virus (TMV) is one of the most studied virus. Its properties (simplicity, self assembly of viral capsid, the ability to proliferate in a large variety of hosts) offers a lot of applications in various scientific or biological fields. Gene vectors based on the tobacco mosaic virus represent a very good way for expressing proteins in plants. Because the tobacco mosaic virus is well researched and a large number of his properties have already been discovered, it is used for nanotechnologic sectors in this period. Not only for the formation of nanoparticles, but also for example for a potential production of vaccines in plants. In this paper I summarize the known information about the process of self assembly of tobacco mosaic virus and the possibility of using this virus in the emerging fields of biology. Key words Tobacco mosaic virus, self assembly, viral vector, nanotechnology
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Viral vectors based on tobacco mosaic virus for the expression of recombinant proteins in plants.
Kroupa, Daniel ; Moravec, Tomáš (advisor) ; Plchová, Helena (referee)
(eng): Over the past few decades, plant viruses and vectors derived from them have become not only an integral part of the work in laboratories, but also began to be used commercially for the production of vaccines, drugs, enzymes and other proteins / peptides. One of the most commonly used viruses for the expression of heterologous proteins is tobacco mosaic virus. In this work, I would like to focus on plant viruses and vectors derived therefrom. Compare it with other expression systems used in plants and describe how their benefits could be further used and combined with modern methods of cloning. Key words: viral vector, expression, Tobacco mosaic virus, Cowpea mosaic virus, Golden gate cloning
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