National Repository of Grey Literature 139 records found  1 - 10nextend  jump to record: Search took 0.04 seconds. 
Patterning of excitable cells on multi-electrode arrays
Slavík, Jan ; doc. RNDr. Petr. Skládal, CSc. (referee) ; Průcha,, Jaroslav (referee) ; Hubálek, Jaromír (advisor)
Práce se zabývá zarovnáváním excitabilních buněk na multielektrodových polích. Nejprve bylo analyzováno zarovnávání excitabilních buněk. Byly použity embryonální neurony z hippocampusu potkanů a HL-1 buňky, které jsou odvozeny z AT-1 linie nádorových myších atriálních kardiomyocytů. Zarovnávání bylo testováno na drážkovaných površích a na površích s materiály s různou buněčnou afinitou. Bylo prokázáno, že na drážkových površích se ve směru drážek zarovnávají neurony i HL-1 buňky, ale na površích s různou chemickou affinitou se zarovnávají pouze neurony. Dále byly vyrobeny vlastní multielektrodová pole, na těchto multielektrodových polích byly kultivovány HL-1 buňky a byl změřen a analyzován akčních potenciál HL-1 buněk. Cílem bylo prokázat, že je možné měřit akční potenciál na vyrobených multielektrodových polích. Pro zarovnání buněk na multielektrodovém poli bylo vyrobeno speciální multieletrodové pole s uniformním povrchem. Toto multielektrodové pole je nazýváno planární multielektrodové pole. Planární multielektrodové pole bylo vyrobeno speciálním vyrobním procesem. Vrstvy planárního multielektrodového pole byly deponovány na pomocný substrát v opačném pořadí. Pomocný substrátem pro depozici byla křemíková deska, na který byla nadeponována další pomocná vrstva zlata. Horní izolační vrstva planárního multielektrodové pole byla deponována jako první a nejspodnější vrstva substrátu byla nadeponována jako poslední. Planární multielektrodové pole i s pomocnou zlatou vrstvou bylo strhnuto s křemíku díky nízké adhezi zlata ke křemíku a planární multielektrodové pole se otočilo vzhůru nohama. Pomocná zlatá vrstva byla odstraněna mokrým leptadlem a tím bylo planární multielektrodové pole dokončeno. Na planárním multielektrodovém poli byly zarovnány HL-1 buňky do pruhů chemickou metodou pomocí kombinace otisku adhezní látky a následným potažení neotisklých ploch anti-adhezní látkou. Elektrofyziologické vlastnosti zarovnaných HL-1 buněk byly změřeny pomocí planárního multielektrodového pole. Tímto experimentem byla představena výrobní technologie pro výrobu planárních multielektrodových polí a toto planární multielektrodové pole bylo úspěšně testováno pro zarovnání HL-1 buněk na jeho povrchu kombinací otisku adhezní látky a potahování antiadhezivním činidlem.
Analysis of the pension funds by means of time series
Heikenwälder, Jakub ; Slavík, Jan (referee) ; Kropáč, Jiří (advisor)
This bachelor thesis disserts on analysis of the pension funds in Czech Republic. It deals with theory of time series and regression analysis. It analyses evaluation of each fund and possibilities of future evaluation's prognosis.
Design of the CSR Concept in the Company Management System
Macháčová, Zuzana ; Slavík, Jan (referee) ; Putnová, Anna (advisor)
The diploma thesis deals with the design of the CSR concept in a selected company. On the basis of theoretical knowledge together with a suitably chosen methodology, the main part of the work follows, where the level of corporate social responsibility in the economic, social and environmental areas is evaluated. The results are followed by a design part, which contains ideas and measures leading to the creation of the company's concept.
Proposal of the Company's Financial Plan
Bednarovičová, Andrea ; Slavík, Jan (referee) ; Žižlavský, Ondřej (advisor)
This master’s thesis deals about topic of financial planning of the company. At first there are defined theoretical ways out of financial planning. The next part analyses contemporary state of the company. Subsequently there is worked out the financial plan for the years 2012-2014 in in realistic, optimistic and pessimistic variant. The end of the thesis forms evaluation of created financial plans.
Assessment of the Impact of Planning on Land Prices
Slavík, Jan ; Šestáková, Romana (referee) ; Doležalová, Monika (advisor)
This diploma thesis describes methodology background for price evaluation of real properties. The case study presents the evaluation of selected land in cadastral area Podolí u Brna, town Podolí in different conditions of town and country planning. The administrative price and market price are obtained from evaluation in three different steps of country planning. The obtained results are interpreted and changes in prices are outlined with an effect of town and country planning changes.
Life cycle assessment of municipal waste
Vlach, Josef ; Slavík, Jan (referee) ; Jícha, Jaroslav (advisor)
The aim of this study is to present methodology life cycle (LCA) and Life Cycle Assessment of disposal the municipal solid waste chain. There is described the procedure of generation of the LCA studies according to the standards ČSN EN ISO 14 040 and ČSN EN ISO 14 044 in the first and second part. In the third part of this diploma there are described LCA analyses that have already been created. The software GEMIS (Global Emission Model for Integrated System) is described in the fourth part. The computer model GEMIS has been used as a calculation tool in this study. The fifth part of this study includes waste management and different kinds of disposal municipal solid waste. In this part is describe basic data about landfilling and incieration. There is shown LCA analysis of disposal municipial solid waste in the sixth part. Data processing was carried out by means of the software GEMIS 4.3. The results were interpreted for incineration and landfilling. Finally there is mentioned results for 1 TJ different elektrical energize to.
Electrochemical and mathematical study of interactions of selenite with biologically active thiols
Slavík, Jan ; Hynek, David (referee) ; Adam, Vojtěch (advisor)
Proteins with thiol groups interact with metal ions in the human body. They maintain their homeostasis, participate in cell signaling, protect the cell against the effects of toxic metals and detoxify them. This work is focused on proteins with thiol groups glutathione and metallothionein and their effects on selenium. The method of study is electrochemical.
Analytical methods on a chip
Slavík, Jan ; Bendová, Mária (referee) ; Hubálek, Jaromír (advisor)
This work deals with fabrication and test of microfluidic chip for separating substances. For separation of substances is used electrophoresis and detection is by integrated electrodes.
Evalution of Transfection Methods for Transfection of COS-7 Cells
Slavík, Jan ; Hošek, Jan (referee) ; Rychtárik, Milan (advisor)
The aim of this work is summarized potentialities of cells transfection in their practise, which offers the Department of biomedical engineering. On COS-7 cell line are compared to those transfection reagents: calcium phosphate, Fugen 6 and Fugen HD.
Spatial perception and its artistic transcription in the professional curriculum program RVP 36-47-M/01 in the field of construction
Koubek Michaličková, Magdalena ; Kafková, Helena (advisor) ; Stempel, Ján (referee) ; Slavík, Jan (referee)
The dissertation delas with the curriculum, its modeling and its research. The designed protocols provide feedback in the case studies of multiple case studies of the implemented curriculum in the Architectural drawing subject. The main tasks were to provide SPŠS students with basic knowledge of three- dimensional drawing, which provided them with the current basics for sketching, including the acquisition of linear perspective. Furthermore, incorporate into the teaching of the current and applicable content of the subject matter from the still valid high school textbook of Professional Drawing. To purposefully develop spatial imagination using tasks where the position of the observer is deliberately changed. To support the creative aspect of the subject in tasks in which students get to know creative techniques. To offer such a wide range of tasks that each of the students, according to their typological inclinations, can find the area in which they can improve further. Key words 3D drawing; architectural drawing; architectural sketching; linear perspective, spatial expression

National Repository of Grey Literature : 139 records found   1 - 10nextend  jump to record:
See also: similar author names
13 SLAVÍK, Jiří
1 Slavík, J.
5 Slavík, Jakub
1 Slavík, Jaroslav
13 Slavík, Jiří
2 Slavík, Josef
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