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Analysis of the meteorological dates
Votavová, Barbora ; Čížek, Martin (referee) ; Rozman, Jiří (advisor)
The aim of this study is to recognize structure of data from the meteorological station, choose useful programme for its analysis and make a theoretical analysis and graphics. The work describes the collection of data from meteorological stations, data stucture, meteorological elements, their measurement and selection of appropriate statistical program. Practical part includes charts with commentary. Conclusion summarizes the results of the analysis.
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Membrane potential measurement with voltage sensitive dyes
Votavová, Barbora ; Janoušek, Oto (referee) ; Čmiel, Vratislav (advisor)
The aim of this work is to realize measurements of membrane potential with voltagesensitive dye Di-4-ANEPPS and the data processed and analyzed. The work includes theoretical basis in the form of electrophysiology animal cells, explains fluorescence and describes the fluorescence microscope. The document is largely devoted to the characterization and distribution of voltage-sensitive dyes (VSD). The practical part deals with the various components necessary to perform the experiment as a pulse generator, high-speed camera and camera’s acquisition and describes experiment. The conclusion will be compared with results from theoretical assumptions.
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The assembly of perinuclear actin stress fibers and their role in cell movement
Votavová, Barbora ; Vomastek, Tomáš (advisor) ; Cvačková, Zuzana (referee)
Nucleus is the largest cellular organelle in animal cells. Due to its bulky nature and the stiffness of nuclear lamina the nucleus constitutes the substantial problem for migrating cells where nucleus has to move. The actomyosin generated forces and LINC (Linker of Nucleoskeleton and Cytoskeleton) complex, that is composed of SUN and nesprin proteins, play key role in nuclear movement. LINC complex mechanically couples nuclear lamina to the cytoskeleton and allows the forces exerted by the cytoskeleton to move the nucleus. Perinuclear actin fibers, also termed actin cap, mechanically link focal adhesions with nucleus and they may generate forces that position the nucleus in a way that is optimal for cellular movement. However, molecular mechanism of how perinuclear actin fibers and LINC complex orchestrate the nuclear movement and functional significance of this process remain poorly understood. The specific aim was to determine the mechanisms by which perinuclear actin fibers are formed and how are these mechanisms employed to facilitate cell migration. The role of LPA-RhoA signaling axis and LINC complex in the formation of perinuclear actin fibers was also examined. It was confirmed that LPA is essencial stimulus during actin cap formation. On the other hand, FAK kinase was found necessary for...
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Activation of the initiation caspases and regulation of their activity
Votavová, Barbora ; Anděra, Ladislav (advisor) ; Černý, Jan (referee)
Caspases are the key proteins participating in both activation and execution of apoptosis. Extrinsic or intrinsic apoptotic signaling leads to sequential activation of the initiation and execution caspases. Activation of initiator caspases is mediated by their processing in multiprotein complexes and activated initiator caspases then specifically cleave and thus activate the effector caspases. These then cleave a number of structural and functional proteins, which consequently leads to cellular selfdestruction and its breakdown to apoptotic bodies. Considering the fundamental significance of the initiation of apoptosis, the activation as well as the activity of initiator (but also effector) caspases is strictly regulated at several levels. Primary the intensity and character of the recieved signal is crucial for the effective formation of the caspase activation complex. Then the concentration of intracellular ionts, nucleotides and various proteins (proteins from the Bcl-2 family, inhibitors of apoptosis (IAPs), heat shock proteins,…) can also highly influence individual steps of caspase activation. Caspases themself can be posttranslationally modified (phosphorylated, ubiquitylated,…) and their activity can be either suppressed or also enhanced. All these processes form complex regulatory network serving...
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Citizen photojournalism - amateurs as photoreporters
Votavová, Barbora ; Láb, Filip (advisor) ; Turek, Pavel (referee)
The bachelor thesis Citizen Photojournalism - Amateurs as Photoreporters deals with increasing citizen participation in creating visual media content which has seen big growth over the last decade thanks to development of communications and information technology. The thesis is divided into three separate parts. The first part focuses on theoretical definition of citizen journalism and photojournalism phenomena. It shows how big impact the new technologies have on journalism amateurisation and also remarks on relationship between amateurs interfering with media and professionals. Second part of the bachelor thesis describes social networks and internet services that contributed to expansion of visual journalism by enabling photo sharing and publishing. Some examples of these include Flickr, Twitter or photography agency Demotix. Third part looks into concrete examples of amateur photos making their way into media as a full-fledged news material - for example London 2005 terrorist attacks or Arab Spring in 2011. All three parts emphasise print and electronic media.
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University studies without a title - drop-out phenomenon in the context of massification
Votavová, Barbora ; Šanderová, Jadwiga (advisor) ; Tuček, Milan (referee)
Barbora Votavová ISS FSV UK Diploma thesis abstract University career without a degree - drop-out phenomenon in the context of university education massification The thesis focuses on the phenomenon of not finishing university in the context of masification of tertiary education and changign character of labour market. Unlike educational reproduction and mobility, that is often studied in Czech context, only a couple of texts focus on the phenomenon of not finishing. This text therefore tries to open this topic by analyzing experiences and narration of those who leave university before getting a degree. Based on interviews with drop-outs, it searches for the specific aspects of their educational trajectories. It attempts to reconstruct the meanings that they ascribe various parts, moments and characteristics of their own educational trajectories. The thesis searches answers to basic questions about the significance and meaning of education, trajectories and aspects influencing the specific shape of the trajectory and identify areas that are relevant for the drop-out topic. Original data are analyzed in the broader context in which the trajectories take place - the context of dynamic and changing labour market and changing character of universities, and interpreted in the context of existing drop-out...
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Analysis of the meteorological dates
Votavová, Barbora ; Čížek, Martin (referee) ; Rozman, Jiří (advisor)
The aim of this study is to recognize structure of data from the meteorological station, choose useful programme for its analysis and make a theoretical analysis and graphics. The work describes the collection of data from meteorological stations, data stucture, meteorological elements, their measurement and selection of appropriate statistical program. Practical part includes charts with commentary. Conclusion summarizes the results of the analysis.
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Membrane potential measurement with voltage sensitive dyes
Votavová, Barbora ; Janoušek, Oto (referee) ; Čmiel, Vratislav (advisor)
The aim of this work is to realize measurements of membrane potential with voltagesensitive dye Di-4-ANEPPS and the data processed and analyzed. The work includes theoretical basis in the form of electrophysiology animal cells, explains fluorescence and describes the fluorescence microscope. The document is largely devoted to the characterization and distribution of voltage-sensitive dyes (VSD). The practical part deals with the various components necessary to perform the experiment as a pulse generator, high-speed camera and camera’s acquisition and describes experiment. The conclusion will be compared with results from theoretical assumptions.
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