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ECG based atrial fibrillation detection
Prokopová, Ivona ; Kolářová, Jana (referee) ; Ronzhina, Marina (advisor)
Atrial fibrillation is one of the most common cardiac rhythm disorders characterized by ever-increasing prevalence and incidence in the Czech Republic and abroad. The incidence of atrial fibrillation is reported at 2-4 % of the population, but due to the often asymptomatic course, the real prevalence is even higher. The aim of this work is to design an algorithm for automatic detection of atrial fibrillation in the ECG record. In the practical part of this work, an algorithm for the detection of atrial fibrillation is proposed. For the detection itself, the k-nearest neighbor method, the support vector method and the multilayer neural network were used to classify ECG signals using features indicating the variability of RR intervals and the presence of the P wave in the ECG recordings. The best detection was achieved by a model using a multilayer neural network classification with two hidden layers. Results of success indicators: Sensitivity 91.23 %, Specificity 99.20 %, PPV 91.23 %, F-measure 91.23 % and Accuracy 98.53 %.
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Inhibice histon-deacatyláz a mitochondriální pohyb v neuronech
Prokopová, Iva ; Pávek, Petr (advisor) ; Červený, Lukáš (referee)
1 Abstract Histone Deacetylase Inhibition and Mitochondrial Trafficking in Living Neurons Mitochondrial trafficking is necessary for proper neuronal function and its impairment leads to neurodegeneration. We have found significant differences in mitochondrial movement between cortical and striatal neurons derived from rat brain. In striatum, mitochondria move with lower average speeds and decreased overal mitochondrial dynamics than those in cortex, exhibiting the same mitochondrial fractional occupancy in neuronal processes. How this could contribute to high striatal vulnerability in neurodegeneration is discussed. We used two different methods of trafficking analysis: manual and semi-automatic. These analyses determine average movement speeds of single mitochondria, as well as overal mitochondrial dynamics in particular field. Only neurons with established synaptic connections were analyzed, mitochondria travelling in both directions were found to move with equivalent average speeds, thus not further distinguished. Our preliminary results also reveal that histone deacetylase inhibition with trichostatin A increases mitochondrial trafficking in striatal neurons, independently of mitochondrial fractional occupancy and Ca2+ levels in neuronal processes. We suggest that possible mechanism of this effect is...
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Neuropharmacology of spatial navigation, cognitive coordination and flexibility tests in animal models
Prokopová, Iva ; Stuchlík, Aleš (advisor) ; Vyklický, Ladislav (referee) ; Blahoš, Jaroslav (referee)
Spatial navigation, cognitive coordination and behavioral flexibility belong amongst cognitive functions, which play a role in many neuropsychiatric disorders. Behavioral tasks have proved to be useful paradigms to test these functions in pharmacological or genetic animal models. First aim was to determine a potential interaction between β-adrenergic and α1-adrenergic or D2-dopaminergic systems. Spatial navigation and coordination were impaired in both studies during co-aplication of subthreshold doses of drugs. Used substances belong to group of widely prescribed drugs, thus our results could be implicated in clinical practice. Another study examined an acute effect of MK-801 (animal model of schizophrenia) on behavioral flexibility in Carousel maze and the Morris water maze (MWM). Carousel maze showed higher sensitivity with impairments from 0.08 mg.kg-1 compared to 0.10 mg.kg- 1 in MWM. The final experiment aimed at testing the effect of reduced expression of Nogo-A protein on spatial navigation and behavioral flexibility of rats. A battery of tests in the Carousel maze revealed impairment in cognitive functions, MWM showed unaffected working memory of rats. Our results support the hypothesis linking Nogo-A knock-down rats with neuropsychiatric symptoms and cognitive disorders. Key words:...
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ECG based atrial fibrillation detection
Prokopová, Ivona ; Kolářová, Jana (referee) ; Ronzhina, Marina (advisor)
Atrial fibrillation is one of the most common cardiac rhythm disorders characterized by ever-increasing prevalence and incidence in the Czech Republic and abroad. The incidence of atrial fibrillation is reported at 2-4 % of the population, but due to the often asymptomatic course, the real prevalence is even higher. The aim of this work is to design an algorithm for automatic detection of atrial fibrillation in the ECG record. In the practical part of this work, an algorithm for the detection of atrial fibrillation is proposed. For the detection itself, the k-nearest neighbor method, the support vector method and the multilayer neural network were used to classify ECG signals using features indicating the variability of RR intervals and the presence of the P wave in the ECG recordings. The best detection was achieved by a model using a multilayer neural network classification with two hidden layers. Results of success indicators: Sensitivity 91.23 %, Specificity 99.20 %, PPV 91.23 %, F-measure 91.23 % and Accuracy 98.53 %.
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Use of the Internet of Things in Supply Chain Management
Prokopová, Iva ; Basl, Josef (advisor) ; Fortinová, Jana (referee)
The thesis deals with the description of possibilities of using the Internet of Things in supply chain management and explores the use of the Internet of Things in the supply chain management among Czech companies. The theoretical part deals with the possibilities of using the Internet of Tings in the supply chain management as well as the developing standards and technologies, which make it possible to use these possibilities. The theoretical part further includes the challenges and risks of the Internet of Things that need to be dealt with when implementing it and the further anticipated development in this area. A survey based on the findings of the theoretical part of the thesis revealed the awareness of Czech businesses about the Internet of Things and how they plan to use it. The practical part of the thesis contains the evaluation of the hypotheses formulated on the basis of the results of this survey and discussion of the facts.
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Neuropharmacology of spatial navigation, cognitive coordination and flexibility tests in animal models
Prokopová, Iva ; Stuchlík, Aleš (advisor) ; Vyklický, Ladislav (referee) ; Blahoš, Jaroslav (referee)
Spatial navigation, cognitive coordination and behavioral flexibility belong amongst cognitive functions, which play a role in many neuropsychiatric disorders. Behavioral tasks have proved to be useful paradigms to test these functions in pharmacological or genetic animal models. First aim was to determine a potential interaction between β-adrenergic and α1-adrenergic or D2-dopaminergic systems. Spatial navigation and coordination were impaired in both studies during co-aplication of subthreshold doses of drugs. Used substances belong to group of widely prescribed drugs, thus our results could be implicated in clinical practice. Another study examined an acute effect of MK-801 (animal model of schizophrenia) on behavioral flexibility in Carousel maze and the Morris water maze (MWM). Carousel maze showed higher sensitivity with impairments from 0.08 mg.kg-1 compared to 0.10 mg.kg- 1 in MWM. The final experiment aimed at testing the effect of reduced expression of Nogo-A protein on spatial navigation and behavioral flexibility of rats. A battery of tests in the Carousel maze revealed impairment in cognitive functions, MWM showed unaffected working memory of rats. Our results support the hypothesis linking Nogo-A knock-down rats with neuropsychiatric symptoms and cognitive disorders. Key words:...
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Systemic Coapplication of α1- a β-adrenergic Antagonists and Their Effects on Rat Cognition
Prokopová, Iva ; Šlamberová, Romana (referee) ; Pávek, Petr (advisor)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Candidate: Mgr. Iva Prokopová Supervisors: RNDr. Aleš Stuchlík, PhD (FgÚ AV ČR, v.v.i.) Doc. PharmDr. Petr Pávek, PhD (FaF, UK) Title of rigorous thesis: Systemic coapplication of α1- a β-adrenergic antagonists and their effects on rat cognition Spatial learning is a widely-studied type of animal behavior, often considered as a model of human higher cognitive functions. Noradrenergic receptors play a modulatory role in vigilance, attention, reward, learning and memory. The present study aimed at studying the effects of separate or combined systemic administration of α1-adrenergic antagonist prazosin (1 and 2 mg/kg) and β-blocker propranolol (5 and 20 mg/kg) on hippocampus-dependent learning of the active allothetic place avoidance (AAPA) task. Both centrally-active drugs impaired spatial learning when administered together, exerting no effect after separate applications. Locomotion was impaired only by combined application of higher doses of both drugs (2 mg/kg prazosin and 20 mg/kg propranolol). These results suggest an in vivo interaction between these two types of receptors in regulation of spatial navigation. Since there are several combined adrenoceptor blockers (antagonizing both α-...
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Inhibice histon-deacatyláz a mitochondriální pohyb v neuronech
Prokopová, Iva ; Pávek, Petr (advisor) ; Červený, Lukáš (referee)
1 Abstract Histone Deacetylase Inhibition and Mitochondrial Trafficking in Living Neurons Mitochondrial trafficking is necessary for proper neuronal function and its impairment leads to neurodegeneration. We have found significant differences in mitochondrial movement between cortical and striatal neurons derived from rat brain. In striatum, mitochondria move with lower average speeds and decreased overal mitochondrial dynamics than those in cortex, exhibiting the same mitochondrial fractional occupancy in neuronal processes. How this could contribute to high striatal vulnerability in neurodegeneration is discussed. We used two different methods of trafficking analysis: manual and semi-automatic. These analyses determine average movement speeds of single mitochondria, as well as overal mitochondrial dynamics in particular field. Only neurons with established synaptic connections were analyzed, mitochondria travelling in both directions were found to move with equivalent average speeds, thus not further distinguished. Our preliminary results also reveal that histone deacetylase inhibition with trichostatin A increases mitochondrial trafficking in striatal neurons, independently of mitochondrial fractional occupancy and Ca2+ levels in neuronal processes. We suggest that possible mechanism of this effect is...
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Internet marketing focused on language institute
Prokopová, Iva ; Smutný, Zdeněk (advisor) ; Matuštík, Ondřej (referee)
This bachelor thesis deals with internet marketing trends for market in the Czech Republic with an accent on a low-cost approach. The bachelor thesis consists of two parts, theoretical and practical. The theoretical part introduces and analyses components, tools and methods of internet marketing, used in the practical part. The practical part contains design, realization and evaluation of an internet marketing campaign for the language institute Mediterin. Particular chapters deal with market analysis, target group definition, search engine optimization, pay-per-click campaigns and web analysis.
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