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The Visual Style of Canoe Races in Týn nad Vltavou
Šafářová, Gabriela ; Marešová, Věra (referee) ; Macháček, Mikuláš (advisor)
I focused on design of information and promotional materials of Canoe Marathon World in Tyn nad Vltavou. I concentrated mainly on the failures of the current visual style and proposed new solutions. The key is to design a new logo and its modifications, which will be put to use in a slight variation in the coming years. Art themes based on specific objects, materials and phenomena (lamina structure, paddles, movement) are simplified into almost abstract forms.
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Penetration of B-lactam antibiotics into cerebrospinal fluid and its relationship to inflammatory markers during invasive bacterial infection
Prášil, Petr ; Buchta, Vladimír (advisor) ; Marešová, Vilma (referee) ; Boštík, Pavel (referee)
Penetration of β-lactam antibiotics into cerebrospinal fluid and its relationship to inflammatory markers during invasive bacterial infection Aim: The penetration of β-lactam antibiotics (ceftriaxone, crystalic penicillin G - potassium salt and cefotaxime) into the cerebrospinal fluid (CSF) was estabilished and the correlation between the penetration and laboratory markers of inflammation was evaluated. Materials and methods: Levels of β-lactam antibiotics in serum and CSF were determined in 24 patients (76 samples). In all patients (n = 24) the CSF/serum ratio for antibiotics was calculated and it was correlated with laboratory markers of inflammation (C-reactive protein, fibrinogen, and CSF numbers of neutrophils). Besides, CSF was examined by a modified bactericidal test in patients (n = 13) with positive bacterial culture. In 14 patients (28 samples) levels of serum antibiotic concentration, both before and after its administration, were measured. Results: Serum levels of ceftriaxone before and after administration (31.2 mg/l ± SD 12.29 and 300.0 mg/l ± SD 125.9, respectively) differed with a statistical significance (p = 0.000156). Further, a significant difference between value of inflammatory markers and value of CSF/serum ratio (Kl/s) of ceftriaxone was observed. Patients with ratio values...
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Inspection quality of social services
Marešová, Veronika ; Angelovská, Olga (advisor) ; Vlčková, Kamila (referee)
Diploma thesis "Inspection of the quality of social services" deals with low quality of social services with measurement of the quality control of social services. Its main objective is to assess the position of the actors (inspectors and providers of social services), social services inspection process and make recommendations for its improvement. The work is characterized by a history of social services, whereon relates social services inspection. The work is based on the principle of subsidiarity, the theory of trust, the theory of government and market failures, as well as information asymmetry theory and the theory of bureaucracy. The diploma thesis also includes quality standards of social services, mark quality and management in social services. Work also includes the very process of inspection of social services. The following chapter is a quality framework for social services. Another part is devoted to the research methods used. At the end of thesis are the recommended areas to alteration which leading to increase efficiency and effectiveness of the process of inspection of social services.
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Interactions of resveratrol derivatives with PXR receptor
Marešová, Veronika ; Pávek, Petr (advisor) ; Trejtnar, František (referee)
Charles University in Prague Department of Pharmacology & Toxicology Student: Supervisor: Prof Title of diploma thesis: Interactions of resveratrol derivatives with PXR receptor Derivatives of resveratrol belong between natural substances contained mainly in red wine and grapefruit. These compounds with stilbene-like structure show antioxidant activity. Pregnane X receptor (PXR) regulates expression of the most important biotransformation enzymes in human liver and intestine including cytochrome P450 3A4 (CYP3A4). Interaction of resveratrol and PXR have already been studied, but published data are contradictory. In my diploma thesis, I studied interaction of resveratrol and its derivatives with PXR. For this purpose, gene reporter assay with quantitative real-time polymerase chain reaction (qRT-PCR) was introduced. It was not possible to conduct experiments in standard way because both resveratrol and its derivatives inhibit lusiferase activities. I studied also effects of resveratrol and its derivatives on expression of CYP3A4 mRNA on differentiated HepaRG cell line. New method gene reporter method with qRT-PCR detection of luciferase was established on hepatoma cell line HepG2 transfected with CYP3A4 reporter construct. Concertation dependent effects of resveratrol and its derivatives were...
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Prognostic indicators of risk of death and the consequences of purulent meningitis
Džupová, Olga ; Janovská, Daniela (advisor) ; Křížová, Pavla (referee) ; Marešová, Vilma (referee) ; Dostál, Václav (referee)
Purulent meningitis is a life-threatening acute infectious disease of the central nervous system. In preantibiotics period was almost one hundred percent fatality rate of disease, with the discovery of antibiotics and their introduction into medical practice in 30th-50th of the last century has decreased dramatically. In recent decades, despite the use of highly effective antibiotics and intensive care procedures, there was no significant reduction in mortality rate and consequences of the disease. Lethal course of the disease affects 15-30% of patients in adulthood. Although not a common infection diagnosis among the top ten infectious causes of death worldwide. The study was based on evaluation of their own group of patients to determine prognostic factors observed history, course of illness and laboratory characteristics of the clinical outcome of disease in adults.
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Adverse reactions after childhood vaccination in the Czech Republic
Kocourková, Aneta ; Dáňová, Jana (advisor) ; Marešová, Vilma (referee) ; Částková, Jitka (referee)
1 ABSTRACT In the Czech Republic a complex study of adverse reactions after vaccination in children has not been presented yet. Even though reporting of serious adverse reactions to the State Institute for Drug Control (SUKL) is mandatory by law, it is seldom performed. Moreover, there is no analysis correlating adverse reactions that occurred at doctors` surgeries with actual reported adverse reactions to SUKL. The main focus of presented work was to analyze and map adverse reactions appearing after vaccinations from the children's vaccination schedule in the Czech Republic. The study was conducted in the form of retrospective-prospective questionnaires concentrating on adverse reactions, their occurrence, character, seriousness, duration and frequency. Furthermore, the questionnaires also focused on socio-demographic data such as gender, age, residency, parents' education, birth weight. Also, the refusal of the compulsory vaccination was assessed. Occurrence of adverse reactions after vaccination in the Czech Republic was analyzed by questionnaires in pediatricians' surgeries (active surveillance). Obtained data were correlated with data available at SUKL (passive surveillance). The study data showed higher number of collected adverse reactions that number of adverse reactions reported to national program...
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Selected aspects of immunopathogenesis of HIV infection
Bartovská, Zofia ; Holub, Michal (advisor) ; Boštík, Pavel (referee) ; Marešová, Vilma (referee)
Introduction: Virus-specific CD8+ T cells are crucial to suppress the viral replication in HIV infection. Their functional status is important as well. Also, the chronic nonspecific immune activation of T and B lymphocytes plays an important role in the immunopathogenesis of HIV infection. Aim of the study: To analyze the frequency and functional status of HIV-specific CD8+ T cells and the expression of nonspecific activation markers on B and T cells in HIV+ patients and to assess the effect of combined antiretroviral therapy (cART) on these parameters. Patients and methods: Our cohort included 80 HIV+ patients: 36 HIV+ patients on cART, 18 patients without therapy, in whom cART was introduced during our study, 9 patients without therapy, 10 patients with primary HIV infection, 3 long-term non-progressors and 4 patients initially on cART, in whom the therapy was discontinued. Control group consisted of 34 HIV- healthy individuals. We examined CD4+ a CD8+ T cell counts, viral load, expression of nonspecific activation markers on T cells and the frequency of HIV-specific CD8+ T cells by ELISpot method and flow cytometry using MHC tetramers and intracellular cytokine detection. Results: No significant differences in HIV-specific CD8+ T cells were found between treated and untreated HIV+ patients. The frequency...
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Diagnostics of neuroinfection caused by human herpesviruses using nucleic acid amplification methods
Labská, Klára ; Marešová, Vilma (advisor) ; Rožnovský, Luděk (referee) ; Krbková, Lenka (referee)
of thesis Diagnostics of neuroinfection caused by human herpesviruses using nucleic acid amplification methods author: MUDr. Klára Labská supervisor: doc. MUDr. Vilma Marešová, CSc. In recent years, the diagnosis of neuroinfections has undergone a shift towards molecular biology methods. Our research focused on the predictive value of the capture of herpesvirus (HV) DNA in cerebrospinal fluid. In the first study, we examined the presence of DNA neurotropic herpes viruses (HSV1, HSV2, VZV and HHV6) in cerebrospinal fluid in immunocompetent patients with laboratory-confirmed tick-borne meningoencephalitis and enterovirus meningitis and meningoencephalitis. The control group consisted of patients with proven absence of an inflammation in the cerebrospinal fluid. Patients were followed for 6 months. The course of the disease and its consequences, including laboratory tests, were compared between groups of patients with and without the presence of HV DNA. In the second study, we tried to demonstrate the presence of HSV1 DNA in cerebrospinal fluid during its symptomatic reactivation in patients with purulent meningitis. In our group of immunocompetent patients with non-purulent inflammation in the cerebrospinal fluid, the proportion of HV DNA positive patients reached 7.5% (13 out of 173), we also...
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