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Characterization of germline alterations affecting genes influencing development and prognosis of specific adult cancers
Jelínková, Sandra ; Kleibl, Zdeněk (advisor) ; Hlaváč, Viktor (referee) ; Hájková, Nikola (referee)
In my dissertation, I studied the genetic predisposition of selected types of cancer that have not been systematically studied in the Czech Republic. We used next-generation panel sequencing to identify germline pathogenic variants. Analysis of 1333 patients with ovarian cancer, 527 patients with endometrial cancer, and 334 patients with hepatocellular carcinoma included sequencing using the CZECANCA panel. A specific CZMELAC panel was prepared for the analysis of 264 melanoma patients. We focused on the identification of pathogenic variants in known predisposition genes. We also evaluated candidate genes and phenotypic characteristics in carriers of pathogenic variants. Analysis of high-risk melanoma patients revealed pathogenic variants in melanoma associated genes in 9/264 (3.4%) patients, and an additional 22 (8.3%) patients carried a pathogenic variant in one of the other predisposition genes. The odds of carrying a pathogenic variant were increased in probands with multiple melanomas and in the presence of melanoma in relatives. The incidence of germline pathogenic variants was highest in ovarian cancer, where pathogenic variants were found in 427/1332 (32.0%) patients, with a predominance of mutations in BRCA1, BRCA2, followed by alterations in other ovarian predisposition genes. Breast and...
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Proposal of the Company's Marketing Mix
Jelínková, Soňa ; Schüller, David (referee) ; Kaňovská, Lucie (advisor)
This diploma thesis deals with a marketing mix of a specific company. In the first part, there is a theoretical base needed for understanding the process. The second part analyses the current situation in the overall business environment. As a result of the research, there is a third part of the diploma thesis suggesting improvements in specific parts of the marketing mix.
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Evaluation of Business Performance Through Financial Analysis Tools
Jelínková, Soňa ; Janíčková, Nikola (referee) ; Ptáček, Roman (advisor)
This bachelor's thesis deals with the evaluation of the efficiency of ZD Výčapy, družstvo for the period 2014-2018 using financial analysis tools. The theoretical part explains the various methods of financial analysis and indicators that are used in this work. In the practical part, selected indicators are applied and with their help, a financial analysis is made. The last part of the thesis focuses on suggestions that could help the collective farm to improve its current situation.
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Time-lapse monitoring of cell mechanical properties
Přibyl, J. ; Rotrekl, V. ; Pešl, M. ; Jelínková, Š. ; Kratochvílová, Irena
Atomic force microscopy (AFM) is a highly sensitive non-invasive surface method able to provide insight into cells' mechanical parameters. Membrane and sub-membrane development, as well as internal cellular properties, can be monitored. The stiffness of cells is a fundamental phenomenon that reflects changes in cell physiology. More importantly, changes in cell mechanical properties are also often found to be closely associated with various disease conditions. Cell mechanics are mainly dependent on cytoskeletal architecture. The development of cryopreserved cells' mechanical properties (stiffness) after thawing was studied using AFM.\n
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Proposal of the Company's Marketing Mix
Jelínková, Soňa ; Schüller, David (referee) ; Kaňovská, Lucie (advisor)
This diploma thesis deals with a marketing mix of a specific company. In the first part, there is a theoretical base needed for understanding the process. The second part analyses the current situation in the overall business environment. As a result of the research, there is a third part of the diploma thesis suggesting improvements in specific parts of the marketing mix.
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Evaluation of Business Performance Through Financial Analysis Tools
Jelínková, Soňa ; Janíčková, Nikola (referee) ; Ptáček, Roman (advisor)
This bachelor's thesis deals with the evaluation of the efficiency of ZD Výčapy, družstvo for the period 2014-2018 using financial analysis tools. The theoretical part explains the various methods of financial analysis and indicators that are used in this work. In the practical part, selected indicators are applied and with their help, a financial analysis is made. The last part of the thesis focuses on suggestions that could help the collective farm to improve its current situation.
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Organisation and composition of outdoor programme for the open air schools
Jelínková, Simona ; Janák, Vladimír (advisor) ; Psohlavec, Lukáš (referee)
Title: Organization and composition of outdoor program for the open air schools Objectives: Main objective of this thesis is to compose suitable outdoor program for open air schools for children groups within age 6 to 10. The goal of this new program is more efficient use of time during open air schools with focus on development of abilities, skills, physical and psychical strength, communication among children and social interaction. Methods: In this thesis will be used quantitative research method, written questioning method. Acquired data analysis will be used as background for development of outdoor program for open air schools for children groups within age 6 to 10. Outcome: Benefit of this thesis will be development of new outdoor program for open air schools for children groups within age 6 to 10 which will be passed on to travel agency offering this kind of programs. Key words: game, child, open air school, nature, teacher, instructor
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The effect of vandetanib, lenvatinib and ellipticine on the expression of rat cytochromes P450 1A and 3A
Jelínková, Sandra ; Dračínská, Helena (advisor) ; Žáková, Lenka (referee)
In recent years, the inhibiition of tyrosine kinases,which may incorrectly regulate some singaling pathway has been used to treat cancer as so-called biological therapy. An example of such inhibitors are vandetanib and lenvatinib. These two substances are used to treat thyroid gland tumors because they affect vascular growth factor receptor or endothelial growth factor receptor that can regulate tumor growth and metastasis. Ellipticine, which has anti-tumor effects on lots of tumor disease, has been investigated in this study together with vandetanib and lenvatinib. In this diploma thesis, the effect of mentioned tyrosine kinase inhibitors, ellipticine and their combinations on gene and protein expression of CYP1A1, 1A2, 3A1 and 3A2 in rat liver in vivo was determined. Protein expression was studied using Western blot method with imunodetection. Gene expression was assessed by quantitative PCR. Moreover, the effect of tested substances and their combinations on CYP1A activity (measured as 7-ethoxyresorufin O-deethylation), CYP1A2 activity (measured as 7-methoxyresorufin O-demethylation), CYP1A1 activity (measured as Sudan I oxidation), CYP3A specific activity (measured as testosteron 6β-hydroxylation) and ellipticine, vandetanib, lenvatinib metabolism was determined. It has been confirmed that...
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Management of the sports organization Bohemians Praha 1905
Jelínková, Simona ; Voráček, Josef (advisor) ; Štědroň, Bohumír (referee)
Title: Management of Boheminas Praha 1905 sports club. Objectives: Main objective of this bachelor thesis is to create suggestions on how to increase efficiency of sport oriented joint-stock company management. Suggestions will be based on current state of management analysis and SWOT analysis. Methods: assisted interview, document analysis, SWOT analysis Outcome: Outcome is consisted of suggestions on how to improve efficiency management. Key words: managerial functions, management, joint-stock company, football club
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